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The map label for this gene is btuB [H]

Identifier: 75676404

GI number: 75676404

Start: 2412121

End: 2414199

Strand: Direct

Name: btuB [H]

Synonym: Nwi_2219

Alternate gene names: 75676404

Gene position: 2412121-2414199 (Clockwise)

Preceding gene: 75676403

Following gene: 75676407

Centisome position: 70.9

GC content: 62.19

Gene sequence:

>2079_bases
ATGTCCGCCGCCTTCGTGTCCGGAGCTTATCGCAGAAGCCTGCTTGCTTCCGCCCTCCTCGCCTCGCTGGGATCGATCGG
CCTGAATACGCCCACAAGCGCGCAGCAATCCGCCTCTCCCAACCTGCTGCCTCCGATCCGGATCGCGCCACCCCCGGATC
GAGCGATGGCGCCCGCACACCGCACGACTTCGCGATCACGCCGTGTCGTCCGCCCCGCTCCCCGGCAAACTCCTGTCGCT
GAGACTCATGTTCCGGGCACGATGGGTTCGACCGTAGTCAGCCCCACGGGAGTGGTGACGCCCGCGGGCCAGCTGGCCAG
CTCCATCACCGTCGTCACCGAGCAGGATATCCAGACCCAGCAACACCGCAGCGTTCCGGATATCCTCAGGACGGCTCCCG
GCCTGAATGTGGTGCAAGCCGGCGGACCTGGCGCCCAGACCTCGATCTTCATGCGAGGCACGAACGCCAACCACACCAAG
GTGATGCTCGACGGAATCGACATCGGCGATCCCGCCAACTCGAATGGCGCGTTCGACTATGCGCACCTTCTCACGGCGGA
CATCCAGCAGATGGAAATCCTCCGGGGCCCGCAGAGCGGACTCTACGGCTCCGACGCGATCGGCGGCGTCATCTCCATCA
TCACGAAAAAGGGCGAAGGTCCGGCGCGGGTCACCGGCTCACTCGAGTCCGGCTCGTTCAAAACCTTCAACCAGACGCTC
GGACTGAGCGGCGCCGAACGGAACTTCAACTACGCGGTCAACGTCGCCCATCTCCATGCCGGAGACGTTCCGGTCACGCC
GCCCGAGCTTCTGCCGCCGGGGCGGCAGGCGATCGGCAACAACTATGACAACATGACTTACTCGACCAAGCTCGGAGTCG
ATCTCAATGAGTATCTGACCGTCAATTCGGTGGTCCGCTATACCGACTCGACGCTGCTGTTCACGGGCGACGGCGGATTT
CCAAGCACTCCCAACGCCAGCCAGAGCACGCACGCCGTCCAGCAACTCTTCAACCGTCAGGAGGCCGTGTGGTCGCTGTT
CGACGGACGCGTCCAGAGCTTCTTCGGTCTCAATTTCACGAACAGCAGAGCCTATGACGTGGGTCCGGGCGACCCGGCCG
CGACGATCACCACGGGAGAACGCCTCAAATTCGACTGGCGCACCGTGACCGAGATTACGCGAGACAACCATCTGATCGTC
GGCGCCGAGCATCAGACCGATCGCATGGACACCGCCGATTTTGCCGCCAGGAACGGCAACAAGGCAGGTTTCGTGCAGTT
GCAATCGGCTTTCGCGGACCGCTTTTTCCTGGTGGCCAATGTCCGCCAGGATTCCAACGACCTGTTCGGCGGCCGCATGA
CTTACCGGATTGCGCCGGCGGTCATTGTTCCCGTTACCGAAACCAAGCTGAAAGCAAGCTACGGCACGGGATTCAAGGCG
CCCTCGCTGAGCCAGTTGTTCCGGGATTATCCGACCTTCAATTTCTTCGCCAATCCCAACCTGCAACCGGAAGACAGCCG
GGGCTTCGACGCCGGTTTCGAGCAACCTTTGTTCAACGACCGCGTGCGTTTCGGCTCGACCTACTTCCGAAACGACATCA
CCAACCTGATCGACTACAATTCCACGTTCACCTCACTGGTGAACGTGAACAGCGCGACGACCGAGGGCACCGAGACTTTC
GTCGCGGCGCAGATCACCGAACGTTTCGGAATCCGCGCGGATTACACCTTCATCCGCGCCGTCAATGCCGCCACCGGCAT
GCAACTGCTGCGCCGGCCCAAGGAGAAATGGAGCGCAACCGCGACCTGGCTTCCGCTTGATGCGTTGACGTTGTCGGCAA
CTTTGGTCCGGGTCAACGACTGGCTCGATGTCACCCGCGACGGGATGGCATCCGGAATCACGGTGCCCGGCTATACGCTG
GTGAACTTGCGCGGCGACTACGCACTCAGCGACCAGGTCAAGGTATTCGGACGGATCGATAATCTCCTGGACTTCCGCTA
CCAGAATCCGACCGGATTCCTCGCGCCGGGCCTCGCGGTTTTCGGCGGCATCCGGGTGGCCAGTTACGGAGTGCGATAG

Upstream 100 bases:

>100_bases
CAGTCGGGGACTACAGACATCCGCTTCATCGGCGCCGCGCAGGCTCCTGTGAAACCTCGTTCGCTGTGACGTGCCACCGA
CGTCCAGCCGAGGTTTTCTT

Downstream 100 bases:

>100_bases
CGCTCGAAGGCCGCTAGAGCATTTTCCGGCGAAGTGGAAACCGGTTCGCGTGAAGAAAACGCGTCAAAACAACAATCTAG
AGCTTCGCTTCTGATTCAAT

Product: TonB-dependent receptor

Products: NA

Alternate protein names: Cobalamin receptor; Outer membrane cobalamin translocator [H]

Number of amino acids: Translated: 692; Mature: 691

Protein sequence:

>692_residues
MSAAFVSGAYRRSLLASALLASLGSIGLNTPTSAQQSASPNLLPPIRIAPPPDRAMAPAHRTTSRSRRVVRPAPRQTPVA
ETHVPGTMGSTVVSPTGVVTPAGQLASSITVVTEQDIQTQQHRSVPDILRTAPGLNVVQAGGPGAQTSIFMRGTNANHTK
VMLDGIDIGDPANSNGAFDYAHLLTADIQQMEILRGPQSGLYGSDAIGGVISIITKKGEGPARVTGSLESGSFKTFNQTL
GLSGAERNFNYAVNVAHLHAGDVPVTPPELLPPGRQAIGNNYDNMTYSTKLGVDLNEYLTVNSVVRYTDSTLLFTGDGGF
PSTPNASQSTHAVQQLFNRQEAVWSLFDGRVQSFFGLNFTNSRAYDVGPGDPAATITTGERLKFDWRTVTEITRDNHLIV
GAEHQTDRMDTADFAARNGNKAGFVQLQSAFADRFFLVANVRQDSNDLFGGRMTYRIAPAVIVPVTETKLKASYGTGFKA
PSLSQLFRDYPTFNFFANPNLQPEDSRGFDAGFEQPLFNDRVRFGSTYFRNDITNLIDYNSTFTSLVNVNSATTEGTETF
VAAQITERFGIRADYTFIRAVNAATGMQLLRRPKEKWSATATWLPLDALTLSATLVRVNDWLDVTRDGMASGITVPGYTL
VNLRGDYALSDQVKVFGRIDNLLDFRYQNPTGFLAPGLAVFGGIRVASYGVR

Sequences:

>Translated_692_residues
MSAAFVSGAYRRSLLASALLASLGSIGLNTPTSAQQSASPNLLPPIRIAPPPDRAMAPAHRTTSRSRRVVRPAPRQTPVA
ETHVPGTMGSTVVSPTGVVTPAGQLASSITVVTEQDIQTQQHRSVPDILRTAPGLNVVQAGGPGAQTSIFMRGTNANHTK
VMLDGIDIGDPANSNGAFDYAHLLTADIQQMEILRGPQSGLYGSDAIGGVISIITKKGEGPARVTGSLESGSFKTFNQTL
GLSGAERNFNYAVNVAHLHAGDVPVTPPELLPPGRQAIGNNYDNMTYSTKLGVDLNEYLTVNSVVRYTDSTLLFTGDGGF
PSTPNASQSTHAVQQLFNRQEAVWSLFDGRVQSFFGLNFTNSRAYDVGPGDPAATITTGERLKFDWRTVTEITRDNHLIV
GAEHQTDRMDTADFAARNGNKAGFVQLQSAFADRFFLVANVRQDSNDLFGGRMTYRIAPAVIVPVTETKLKASYGTGFKA
PSLSQLFRDYPTFNFFANPNLQPEDSRGFDAGFEQPLFNDRVRFGSTYFRNDITNLIDYNSTFTSLVNVNSATTEGTETF
VAAQITERFGIRADYTFIRAVNAATGMQLLRRPKEKWSATATWLPLDALTLSATLVRVNDWLDVTRDGMASGITVPGYTL
VNLRGDYALSDQVKVFGRIDNLLDFRYQNPTGFLAPGLAVFGGIRVASYGVR
>Mature_691_residues
SAAFVSGAYRRSLLASALLASLGSIGLNTPTSAQQSASPNLLPPIRIAPPPDRAMAPAHRTTSRSRRVVRPAPRQTPVAE
THVPGTMGSTVVSPTGVVTPAGQLASSITVVTEQDIQTQQHRSVPDILRTAPGLNVVQAGGPGAQTSIFMRGTNANHTKV
MLDGIDIGDPANSNGAFDYAHLLTADIQQMEILRGPQSGLYGSDAIGGVISIITKKGEGPARVTGSLESGSFKTFNQTLG
LSGAERNFNYAVNVAHLHAGDVPVTPPELLPPGRQAIGNNYDNMTYSTKLGVDLNEYLTVNSVVRYTDSTLLFTGDGGFP
STPNASQSTHAVQQLFNRQEAVWSLFDGRVQSFFGLNFTNSRAYDVGPGDPAATITTGERLKFDWRTVTEITRDNHLIVG
AEHQTDRMDTADFAARNGNKAGFVQLQSAFADRFFLVANVRQDSNDLFGGRMTYRIAPAVIVPVTETKLKASYGTGFKAP
SLSQLFRDYPTFNFFANPNLQPEDSRGFDAGFEQPLFNDRVRFGSTYFRNDITNLIDYNSTFTSLVNVNSATTEGTETFV
AAQITERFGIRADYTFIRAVNAATGMQLLRRPKEKWSATATWLPLDALTLSATLVRVNDWLDVTRDGMASGITVPGYTLV
NLRGDYALSDQVKVFGRIDNLLDFRYQNPTGFLAPGLAVFGGIRVASYGVR

Specific function: Involved in the active translocation of vitamin B12 (cyanocobalamin) across the outer membrane to the periplasmic space. It derives its energy for transport by interacting with the trans-periplasmic membrane protein TonB [H]

COG id: COG4206

COG function: function code H; Outer membrane cobalamin receptor protein

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TonB-dependent receptor family. BtuB (TC 1.B.14.3.1) subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790405, Length=614, Percent_Identity=27.3615635179153, Blast_Score=170, Evalue=2e-43,
Organism=Escherichia coli, GI1788478, Length=128, Percent_Identity=33.59375, Blast_Score=81, Evalue=3e-16,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012910
- InterPro:   IPR000531
- InterPro:   IPR010101
- InterPro:   IPR010917 [H]

Pfam domain/function: PF07715 Plug; PF00593 TonB_dep_Rec [H]

EC number: NA

Molecular weight: Translated: 74792; Mature: 74661

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAFVSGAYRRSLLASALLASLGSIGLNTPTSAQQSASPNLLPPIRIAPPPDRAMAPAH
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCEECCCCCCCCCCCH
RTTSRSRRVVRPAPRQTPVAETHVPGTMGSTVVSPTGVVTPAGQLASSITVVTEQDIQTQ
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHCEEEEEECCHHHHH
QHRSVPDILRTAPGLNVVQAGGPGAQTSIFMRGTNANHTKVMLDGIDIGDPANSNGAFDY
HHCCHHHHHHCCCCCCEEECCCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCHH
AHLLTADIQQMEILRGPQSGLYGSDAIGGVISIITKKGEGPARVTGSLESGSFKTFNQTL
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHEEEEECCCCCEEEEECCCCCCCEEHHHHH
GLSGAERNFNYAVNVAHLHAGDVPVTPPELLPPGRQAIGNNYDNMTYSTKLGVDLNEYLT
CCCCCCCCCCEEEEEEEEECCCCCCCCHHCCCCCHHHHCCCCCCCEEEEEECCCHHHHEE
VNSVVRYTDSTLLFTGDGGFPSTPNASQSTHAVQQLFNRQEAVWSLFDGRVQSFFGLNFT
HHHHEEECCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
NSRAYDVGPGDPAATITTGERLKFDWRTVTEITRDNHLIVGAEHQTDRMDTADFAARNGN
CCEEEECCCCCCCEEEECCCEEEECHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHCCCC
KAGFVQLQSAFADRFFLVANVRQDSNDLFGGRMTYRIAPAVIVPVTETKLKASYGTGFKA
CCEEEEEHHHHHCEEEEEEECCCCCCCCCCCEEEEEECCEEEEEECHHEEEECCCCCCCC
PSLSQLFRDYPTFNFFANPNLQPEDSRGFDAGFEQPLFNDRVRFGSTYFRNDITNLIDYN
CCHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHCCC
STFTSLVNVNSATTEGTETFVAAQITERFGIRADYTFIRAVNAATGMQLLRRPKEKWSAT
CHHHHEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHCCHHHHCCE
ATWLPLDALTLSATLVRVNDWLDVTRDGMASGITVPGYTLVNLRGDYALSDQVKVFGRID
EEEEEHHHHEEEEEEEEEHHHHHHHHCHHHCCCCCCCEEEEEECCCEEECCHHHHHHHHH
NLLDFRYQNPTGFLAPGLAVFGGIRVASYGVR
HHHHEEECCCCCCCCCHHHHHCCCEEEECCCC
>Mature Secondary Structure 
SAAFVSGAYRRSLLASALLASLGSIGLNTPTSAQQSASPNLLPPIRIAPPPDRAMAPAH
CCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHCCCCCCCCCCEECCCCCCCCCCCH
RTTSRSRRVVRPAPRQTPVAETHVPGTMGSTVVSPTGVVTPAGQLASSITVVTEQDIQTQ
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCHHHHCEEEEEECCHHHHH
QHRSVPDILRTAPGLNVVQAGGPGAQTSIFMRGTNANHTKVMLDGIDIGDPANSNGAFDY
HHCCHHHHHHCCCCCCEEECCCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCCCCCCHH
AHLLTADIQQMEILRGPQSGLYGSDAIGGVISIITKKGEGPARVTGSLESGSFKTFNQTL
HHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHEEEEECCCCCEEEEECCCCCCCEEHHHHH
GLSGAERNFNYAVNVAHLHAGDVPVTPPELLPPGRQAIGNNYDNMTYSTKLGVDLNEYLT
CCCCCCCCCCEEEEEEEEECCCCCCCCHHCCCCCHHHHCCCCCCCEEEEEECCCHHHHEE
VNSVVRYTDSTLLFTGDGGFPSTPNASQSTHAVQQLFNRQEAVWSLFDGRVQSFFGLNFT
HHHHEEECCCEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
NSRAYDVGPGDPAATITTGERLKFDWRTVTEITRDNHLIVGAEHQTDRMDTADFAARNGN
CCEEEECCCCCCCEEEECCCEEEECHHHHHHHCCCCEEEEECCCCCCCCCCHHHHHCCCC
KAGFVQLQSAFADRFFLVANVRQDSNDLFGGRMTYRIAPAVIVPVTETKLKASYGTGFKA
CCEEEEEHHHHHCEEEEEEECCCCCCCCCCCEEEEEECCEEEEEECHHEEEECCCCCCCC
PSLSQLFRDYPTFNFFANPNLQPEDSRGFDAGFEQPLFNDRVRFGSTYFRNDITNLIDYN
CCHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHCCCHHHHHHHHHHHCCC
STFTSLVNVNSATTEGTETFVAAQITERFGIRADYTFIRAVNAATGMQLLRRPKEKWSAT
CHHHHEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHCCHHHHCCE
ATWLPLDALTLSATLVRVNDWLDVTRDGMASGITVPGYTLVNLRGDYALSDQVKVFGRID
EEEEEHHHHEEEEEEEEEHHHHHHHHCHHHCCCCCCCEEEEEECCCEEECCHHHHHHHHH
NLLDFRYQNPTGFLAPGLAVFGGIRVASYGVR
HHHHEEECCCCCCCCCHHHHHCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA