BacMap

Definition	Nitrobacter winogradskyi Nb-255, complete genome.
Accession	NC_007406
Length	3,402,093

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The map label for this gene is braF [H]

Identifier: 75676396

GI number: 75676396

Start: 2403978

End: 2404814

Strand: Direct

Name: braF [H]

Synonym: Nwi_2211

Alternate gene names: 75676396

Gene position: 2403978-2404814 (Clockwise)

Preceding gene: 75676395

Following gene: 75676397

Centisome position: 70.66

GC content: 66.67

Gene sequence:

>837_bases
ATGACGACGCCTGCTCCCATTCTCGACGTCGATGGACTGACGATGCGGTTCGGAGGCATTCTCGCCATCAACGAGCTGTC
GTTCACCGCGCAGCGCCGCCACATCACCGCGCTGATCGGGCCGAACGGCGCGGGCAAGACAACGGTGTTCAACTGCGTCA
CCGGGTTCTACCGGCCCTCGCGAGGCGCCATGCGCCTGGTGCACGATGACGGCGCGACCTTTCGGCTCGATCGCCTGCGC
GACTTCCGCATCTGCAAATACGCCAAGGTGGCGCGTACCTTCCAGAACATCCGCCTGTTTCCCGGCATGACGGCGCTGGA
GAACCTGATGGTCGCGCAGCACAATGCGTTGATGCGCGCGTCCGGTTTCAGCCTGCTCGGCCTGATCGGCGCCCCGTCGT
TCCGGGCCGCGGAGAAGCAGGCAATCGATTCCGCGCGCGACTGGCTCGACCGCATCGGCCTGCTGCAACGCGCCGACGAC
GCCGCGGGCAACCTCCCCTACGGCGACCAGCGCCGCCTGGAGATCGCGCGCGCAATGTGCGCGCAGCCCGCGCTGCTCTG
CCTCGATGAGCCGGCCGCGGGGCTCAACGCCAACGAGAGCGCGGGCTTGAGCGAGCTGTTGCTGTCGATCCGCAGCGACT
TCGGCACGTCTATCCTTCTGATCGAGCACGACATGAGCGTGGTGATGGAAATCTCCGACCACATCATTGTGCTCGACCAC
GGCGTCAAGATCGCCGACGGCACGCCACGCGCGGTGCGCGACGATCCGAAAGTCGTCGCTGCCTATCTCGGCGCCGACGA
GGACACCGCCGCCGCCGTGATGGAGGGCGACGTATGA

Upstream 100 bases:

>100_bases
TGATCTGGCGGCCGCGCGGTCTCGTCAGCCATCGCGCTCCCACCGTGTTCCTGCAAAAACCGACCGAGATATCATCGTCG
CTGGTCAAGGAAGGCCGCGG

Downstream 100 bases:

>100_bases
ACGCCGGCGCAACGCCCCTGCTCGCCATTCGCGGGCTGCGCGCCGCCTATGGCAAGATCGAAGCCCTGAAAGGCATCGAT
CTCGAAATCCAGGCCGGCGA

Product: ABC transporter, ATPase subunit

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 278; Mature: 277

Protein sequence:

>278_residues
MTTPAPILDVDGLTMRFGGILAINELSFTAQRRHITALIGPNGAGKTTVFNCVTGFYRPSRGAMRLVHDDGATFRLDRLR
DFRICKYAKVARTFQNIRLFPGMTALENLMVAQHNALMRASGFSLLGLIGAPSFRAAEKQAIDSARDWLDRIGLLQRADD
AAGNLPYGDQRRLEIARAMCAQPALLCLDEPAAGLNANESAGLSELLLSIRSDFGTSILLIEHDMSVVMEISDHIIVLDH
GVKIADGTPRAVRDDPKVVAAYLGADEDTAAAVMEGDV

Sequences:

>Translated_278_residues
MTTPAPILDVDGLTMRFGGILAINELSFTAQRRHITALIGPNGAGKTTVFNCVTGFYRPSRGAMRLVHDDGATFRLDRLR
DFRICKYAKVARTFQNIRLFPGMTALENLMVAQHNALMRASGFSLLGLIGAPSFRAAEKQAIDSARDWLDRIGLLQRADD
AAGNLPYGDQRRLEIARAMCAQPALLCLDEPAAGLNANESAGLSELLLSIRSDFGTSILLIEHDMSVVMEISDHIIVLDH
GVKIADGTPRAVRDDPKVVAAYLGADEDTAAAVMEGDV
>Mature_277_residues
TTPAPILDVDGLTMRFGGILAINELSFTAQRRHITALIGPNGAGKTTVFNCVTGFYRPSRGAMRLVHDDGATFRLDRLRD
FRICKYAKVARTFQNIRLFPGMTALENLMVAQHNALMRASGFSLLGLIGAPSFRAAEKQAIDSARDWLDRIGLLQRADDA
AGNLPYGDQRRLEIARAMCAQPALLCLDEPAAGLNANESAGLSELLLSIRSDFGTSILLIEHDMSVVMEISDHIIVLDHG
VKIADGTPRAVRDDPKVVAAYLGADEDTAAAVMEGDV

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG0411

COG function: function code E; ABC-type branched-chain amino acid transport systems, ATPase component

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transporter domain [H]

Homologues:

Organism=Homo sapiens, GI9665248, Length=251, Percent_Identity=27.4900398406374, Blast_Score=82, Evalue=7e-16,
Organism=Homo sapiens, GI31657092, Length=252, Percent_Identity=26.5873015873016, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI27881501, Length=238, Percent_Identity=26.0504201680672, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI30795238, Length=238, Percent_Identity=26.0504201680672, Blast_Score=76, Evalue=3e-14,
Organism=Homo sapiens, GI105990541, Length=233, Percent_Identity=27.0386266094421, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1789864, Length=259, Percent_Identity=53.2818532818533, Blast_Score=270, Evalue=6e-74,
Organism=Escherichia coli, GI87081709, Length=265, Percent_Identity=28.3018867924528, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1787029, Length=253, Percent_Identity=24.901185770751, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1786872, Length=254, Percent_Identity=25.5905511811024, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1787370, Length=273, Percent_Identity=27.1062271062271, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1789672, Length=253, Percent_Identity=22.9249011857708, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1788225, Length=257, Percent_Identity=26.4591439688716, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1788210, Length=266, Percent_Identity=27.0676691729323, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI1786563, Length=268, Percent_Identity=25.7462686567164, Blast_Score=88, Evalue=5e-19,
Organism=Escherichia coli, GI1786398, Length=238, Percent_Identity=28.1512605042017, Blast_Score=88, Evalue=6e-19,
Organism=Escherichia coli, GI87082268, Length=263, Percent_Identity=28.8973384030418, Blast_Score=87, Evalue=8e-19,
Organism=Escherichia coli, GI1790525, Length=251, Percent_Identity=24.3027888446215, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788761, Length=260, Percent_Identity=25.3846153846154, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI1788450, Length=267, Percent_Identity=23.9700374531835, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI87081791, Length=268, Percent_Identity=24.6268656716418, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI48995001, Length=267, Percent_Identity=26.9662921348315, Blast_Score=86, Evalue=4e-18,
Organism=Escherichia coli, GI1789032, Length=250, Percent_Identity=26.8, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1789593, Length=264, Percent_Identity=26.5151515151515, Blast_Score=85, Evalue=6e-18,
Organism=Escherichia coli, GI1788644, Length=258, Percent_Identity=25.968992248062, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790190, Length=252, Percent_Identity=24.6031746031746, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788165, Length=261, Percent_Identity=25.6704980842912, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1787712, Length=259, Percent_Identity=24.3243243243243, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI48994997, Length=259, Percent_Identity=24.3243243243243, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787759, Length=266, Percent_Identity=28.9473684210526, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789991, Length=242, Percent_Identity=26.8595041322314, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787089, Length=243, Percent_Identity=23.4567901234568, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI87081782, Length=262, Percent_Identity=27.4809160305344, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1786703, Length=208, Percent_Identity=25.9615384615385, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI87082267, Length=250, Percent_Identity=26, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1790162, Length=241, Percent_Identity=25.7261410788382, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1790544, Length=246, Percent_Identity=25.2032520325203, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1788897, Length=247, Percent_Identity=25.9109311740891, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI145693107, Length=263, Percent_Identity=25.8555133079848, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1789963, Length=261, Percent_Identity=25.2873563218391, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787164, Length=236, Percent_Identity=25.4237288135593, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1788472, Length=269, Percent_Identity=25.2788104089219, Blast_Score=72, Evalue=6e-14,
Organism=Escherichia coli, GI1789873, Length=229, Percent_Identity=27.0742358078603, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI48994943, Length=240, Percent_Identity=25.8333333333333, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1790467, Length=260, Percent_Identity=21.9230769230769, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87081834, Length=239, Percent_Identity=24.2677824267782, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787105, Length=211, Percent_Identity=27.4881516587678, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1787548, Length=263, Percent_Identity=25.4752851711027, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1789962, Length=245, Percent_Identity=24.0816326530612, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1789891, Length=248, Percent_Identity=26.6129032258064, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787758, Length=248, Percent_Identity=24.1935483870968, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1786345, Length=240, Percent_Identity=25.8333333333333, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787792, Length=277, Percent_Identity=23.826714801444, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789586, Length=254, Percent_Identity=25.1968503937008, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI48994883, Length=270, Percent_Identity=25.9259259259259, Blast_Score=65, Evalue=6e-12,
Organism=Escherichia coli, GI1790534, Length=215, Percent_Identity=26.9767441860465, Blast_Score=62, Evalue=5e-11,
Organism=Caenorhabditis elegans, GI17510237, Length=243, Percent_Identity=26.7489711934156, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI71983523, Length=237, Percent_Identity=26.5822784810127, Blast_Score=72, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71983510, Length=237, Percent_Identity=26.5822784810127, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI71983516, Length=237, Percent_Identity=26.5822784810127, Blast_Score=71, Evalue=5e-13,
Organism=Caenorhabditis elegans, GI115533608, Length=230, Percent_Identity=29.1304347826087, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6319856, Length=260, Percent_Identity=24.6153846153846, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24666092, Length=262, Percent_Identity=27.0992366412214, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI221512771, Length=236, Percent_Identity=28.3898305084746, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24648314, Length=247, Percent_Identity=24.6963562753036, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24643674, Length=242, Percent_Identity=25.6198347107438, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19920532, Length=218, Percent_Identity=24.3119266055046, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI24580930, Length=218, Percent_Identity=24.3119266055046, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28574533, Length=219, Percent_Identity=26.027397260274, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI28574150, Length=238, Percent_Identity=26.0504201680672, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI116007328, Length=238, Percent_Identity=26.0504201680672, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24581191, Length=223, Percent_Identity=25.1121076233184, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24581189, Length=223, Percent_Identity=25.1121076233184, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24581193, Length=223, Percent_Identity=25.1121076233184, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI24581195, Length=223, Percent_Identity=25.1121076233184, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI161076682, Length=214, Percent_Identity=26.1682242990654, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI161076684, Length=214, Percent_Identity=26.1682242990654, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24581617, Length=214, Percent_Identity=26.1682242990654, Blast_Score=65, Evalue=5e-11,
Organism=Drosophila melanogaster, GI24581615, Length=214, Percent_Identity=26.1682242990654, Blast_Score=65, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003439
- InterPro:   IPR017871
- InterPro:   IPR003593 [H]

Pfam domain/function: PF00005 ABC_tran [H]

EC number: NA

Molecular weight: Translated: 30079; Mature: 29948

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: PS50893 ABC_TRANSPORTER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPAPILDVDGLTMRFGGILAINELSFTAQRRHITALIGPNGAGKTTVFNCVTGFYRPS
CCCCCCEEECCCCEEECCCEEEEECCHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCC
RGAMRLVHDDGATFRLDRLRDFRICKYAKVARTFQNIRLFPGMTALENLMVAQHNALMRA
CCCEEEEECCCCEEEHHHCCCHHHHHHHHHHHHHHHCEECCCHHHHHHHHHHHHHHHHHC
SGFSLLGLIGAPSFRAAEKQAIDSARDWLDRIGLLQRADDAAGNLPYGDQRRLEIARAMC
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
AQPALLCLDEPAAGLNANESAGLSELLLSIRSDFGTSILLIEHDMSVVMEISDHIIVLDH
CCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCEEEEEECCCEEEEEC
GVKIADGTPRAVRDDPKVVAAYLGADEDTAAAVMEGDV
CEEEECCCCCCCCCCHHEEEEEECCCCCCHHHEECCCC
>Mature Secondary Structure 
TTPAPILDVDGLTMRFGGILAINELSFTAQRRHITALIGPNGAGKTTVFNCVTGFYRPS
CCCCCEEECCCCEEECCCEEEEECCHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCC
RGAMRLVHDDGATFRLDRLRDFRICKYAKVARTFQNIRLFPGMTALENLMVAQHNALMRA
CCCEEEEECCCCEEEHHHCCCHHHHHHHHHHHHHHHCEECCCHHHHHHHHHHHHHHHHHC
SGFSLLGLIGAPSFRAAEKQAIDSARDWLDRIGLLQRADDAAGNLPYGDQRRLEIARAMC
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
AQPALLCLDEPAAGLNANESAGLSELLLSIRSDFGTSILLIEHDMSVVMEISDHIIVLDH
CCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCEEEEEECCCEEEEEC
GVKIADGTPRAVRDDPKVVAAYLGADEDTAAAVMEGDV
CEEEECCCCCCCCCCHHEEEEEECCCCCCHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]