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The map label for this gene is braE [H]

Identifier: 75676395

GI number: 75676395

Start: 2402611

End: 2403981

Strand: Direct

Name: braE [H]

Synonym: Nwi_2210

Alternate gene names: 75676395

Gene position: 2402611-2403981 (Clockwise)

Preceding gene: 75676394

Following gene: 75676396

Centisome position: 70.62

GC content: 64.19

Gene sequence:

>1371_bases
TTGAGCCAACAAACCGCCACGCGCGATCGTGGTGTCGCGGCCATCATCAAGAACGCGGTCGTGAGCGCGCTGCTGGCGCT
GGTGCTGTTCTCACTCATGATCGGCATCCGCACCGAAACCGGCCCATCCGGACAGTTGACCTACTGGACCCGGTTCGGCG
AACTCGCGGCCGTCGTCGGCGCGGTATTCATCGGCAGCATCATCGTCGAAACAATCAAGCGCTGGACCGGCCCCGTCGGC
AGCGTCGAACTGCCGGATACCGTGCGCCGTGCATTCGCAATGCTGGGCCGCCTGCTGATACCCGCGCTCCTGATTTTCGC
CCTGCTGGTGCCGGTCATCTTCTATAATCAGCGCTACCTGCTCGACCTCGCGATCCTCGTGCTGACCTATGTGATGCTGG
GTTGGGGGCTCAACGTCGTGGTCGGTCTCGCCGGACTTCTCGATCTCGGCTACGTGGCGTTCTATGCCGTCGGCGCGTAC
TCCTACGCGCTGCTGGCGCAGAGCGGGCTGCCGTTTCCGTATTTTATCGAGACCTATCCCTACGTCGCGATCGACTTCCT
GCAACTTGGTTCGTGGGCGTTCTGGGCGCTGTTGCCGGTTTCCGGCCTGCTGGCCGCATTCTGGGGGGTGCTGCTCGGCT
TCCCGGTGTTGCGGCTTCGCGGCGACTATCTCGCGATCGTCACGCTGGCGTTCGGGGAAATCATACGGCTTGTCATCCTC
AACTGGCAGGATCTCACCGGCGGGCCGAACGGGATCAGCGGCATCCCGCGCCCCACCGTGTTCGGCATTCCCCTCACGCG
GGGCGACGACGGCTTCGCGGCGCTGATCGGCGTTCCGTTCTCGCCCACGCATCGCCTCGTCTTCCTGTTCTACATCATCC
TTGCGCTAGCGCTGCTGACGAACTGGGTCACCATCCGCCTGCGGCGGCTGCCGATCGGCCGCGCCTGGGAAGCGATGCGC
GAGGATGAGGTGGCCTGCCGCGCGCTCGGCATCAACATCACCACAACGAAACTCTCGGCCTTCGCGATAGGCGCGATGTT
CGGCGGCTTCGCGGGCGCGTTCTTCGCGACCCGGCAGGGCTTCATCAGCCCTGAGTCCTTCACCTTTCAGGAATCGGCGC
TGGTGCTCGCCATCGTCGTCCTGGGCGGCATGGGCTCGCAACTCGGCGTGGCGCTCGCCGCGATCGCAATGATCGGTGGA
TTCGAGTTGTTCCGCGGCCTTGACCAGTTTCGTATGCTGGTGTTCGGCGGCGTCATGGTGCTGCTGATGATCTGGCGGCC
GCGCGGTCTCGTCAGCCATCGCGCTCCCACCGTGTTCCTGCAAAAACCGACCGAGATATCATCGTCGCTGGTCAAGGAAG
GCCGCGGATGA

Upstream 100 bases:

>100_bases
TTCTCGATCCTGATCATCGTGCTGATCTTCATGCCTACCGGCCTGCTTGGCCGTCCCGAAGTCGAAAAAGTCTGACGGTC
CGGACGTGACAGCGCCCGCT

Downstream 100 bases:

>100_bases
CGACGCCTGCTCCCATTCTCGACGTCGATGGACTGACGATGCGGTTCGGAGGCATTCTCGCCATCAACGAGCTGTCGTTC
ACCGCGCAGCGCCGCCACAT

Product: ABC-type branched-chain amino acid transport system permease component

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 455

Protein sequence:

>456_residues
MSQQTATRDRGVAAIIKNAVVSALLALVLFSLMIGIRTETGPSGQLTYWTRFGELAAVVGAVFIGSIIVETIKRWTGPVG
SVELPDTVRRAFAMLGRLLIPALLIFALLVPVIFYNQRYLLDLAILVLTYVMLGWGLNVVVGLAGLLDLGYVAFYAVGAY
SYALLAQSGLPFPYFIETYPYVAIDFLQLGSWAFWALLPVSGLLAAFWGVLLGFPVLRLRGDYLAIVTLAFGEIIRLVIL
NWQDLTGGPNGISGIPRPTVFGIPLTRGDDGFAALIGVPFSPTHRLVFLFYIILALALLTNWVTIRLRRLPIGRAWEAMR
EDEVACRALGINITTTKLSAFAIGAMFGGFAGAFFATRQGFISPESFTFQESALVLAIVVLGGMGSQLGVALAAIAMIGG
FELFRGLDQFRMLVFGGVMVLLMIWRPRGLVSHRAPTVFLQKPTEISSSLVKEGRG

Sequences:

>Translated_456_residues
MSQQTATRDRGVAAIIKNAVVSALLALVLFSLMIGIRTETGPSGQLTYWTRFGELAAVVGAVFIGSIIVETIKRWTGPVG
SVELPDTVRRAFAMLGRLLIPALLIFALLVPVIFYNQRYLLDLAILVLTYVMLGWGLNVVVGLAGLLDLGYVAFYAVGAY
SYALLAQSGLPFPYFIETYPYVAIDFLQLGSWAFWALLPVSGLLAAFWGVLLGFPVLRLRGDYLAIVTLAFGEIIRLVIL
NWQDLTGGPNGISGIPRPTVFGIPLTRGDDGFAALIGVPFSPTHRLVFLFYIILALALLTNWVTIRLRRLPIGRAWEAMR
EDEVACRALGINITTTKLSAFAIGAMFGGFAGAFFATRQGFISPESFTFQESALVLAIVVLGGMGSQLGVALAAIAMIGG
FELFRGLDQFRMLVFGGVMVLLMIWRPRGLVSHRAPTVFLQKPTEISSSLVKEGRG
>Mature_455_residues
SQQTATRDRGVAAIIKNAVVSALLALVLFSLMIGIRTETGPSGQLTYWTRFGELAAVVGAVFIGSIIVETIKRWTGPVGS
VELPDTVRRAFAMLGRLLIPALLIFALLVPVIFYNQRYLLDLAILVLTYVMLGWGLNVVVGLAGLLDLGYVAFYAVGAYS
YALLAQSGLPFPYFIETYPYVAIDFLQLGSWAFWALLPVSGLLAAFWGVLLGFPVLRLRGDYLAIVTLAFGEIIRLVILN
WQDLTGGPNGISGIPRPTVFGIPLTRGDDGFAALIGVPFSPTHRLVFLFYIILALALLTNWVTIRLRRLPIGRAWEAMRE
DEVACRALGINITTTKLSAFAIGAMFGGFAGAFFATRQGFISPESFTFQESALVLAIVVLGGMGSQLGVALAAIAMIGGF
ELFRGLDQFRMLVFGGVMVLLMIWRPRGLVSHRAPTVFLQKPTEISSSLVKEGRG

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=334, Percent_Identity=53.2934131736527, Blast_Score=320, Evalue=9e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 49450; Mature: 49318

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQTATRDRGVAAIIKNAVVSALLALVLFSLMIGIRTETGPSGQLTYWTRFGELAAVVG
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHH
AVFIGSIIVETIKRWTGPVGSVELPDTVRRAFAMLGRLLIPALLIFALLVPVIFYNQRYL
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LDLAILVLTYVMLGWGLNVVVGLAGLLDLGYVAFYAVGAYSYALLAQSGLPFPYFIETYP
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCC
YVAIDFLQLGSWAFWALLPVSGLLAAFWGVLLGFPVLRLRGDYLAIVTLAFGEIIRLVIL
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHH
NWQDLTGGPNGISGIPRPTVFGIPLTRGDDGFAALIGVPFSPTHRLVFLFYIILALALLT
CCHHCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
NWVTIRLRRLPIGRAWEAMREDEVACRALGINITTTKLSAFAIGAMFGGFAGAFFATRQG
HHHHHHHEECCCCHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCC
FISPESFTFQESALVLAIVVLGGMGSQLGVALAAIAMIGGFELFRGLDQFRMLVFGGVMV
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLMIWRPRGLVSHRAPTVFLQKPTEISSSLVKEGRG
HHHHHCCCCCHHCCCCEEEEECCHHHHHHHHHCCCC
>Mature Secondary Structure 
SQQTATRDRGVAAIIKNAVVSALLALVLFSLMIGIRTETGPSGQLTYWTRFGELAAVVG
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHHHHH
AVFIGSIIVETIKRWTGPVGSVELPDTVRRAFAMLGRLLIPALLIFALLVPVIFYNQRYL
HHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
LDLAILVLTYVMLGWGLNVVVGLAGLLDLGYVAFYAVGAYSYALLAQSGLPFPYFIETYP
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHCCC
YVAIDFLQLGSWAFWALLPVSGLLAAFWGVLLGFPVLRLRGDYLAIVTLAFGEIIRLVIL
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHHH
NWQDLTGGPNGISGIPRPTVFGIPLTRGDDGFAALIGVPFSPTHRLVFLFYIILALALLT
CCHHCCCCCCCCCCCCCCCEEEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHH
NWVTIRLRRLPIGRAWEAMREDEVACRALGINITTTKLSAFAIGAMFGGFAGAFFATRQG
HHHHHHHEECCCCHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHCC
FISPESFTFQESALVLAIVVLGGMGSQLGVALAAIAMIGGFELFRGLDQFRMLVFGGVMV
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLMIWRPRGLVSHRAPTVFLQKPTEISSSLVKEGRG
HHHHHCCCCCHHCCCCEEEEECCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]