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The map label for this gene is purB [H]

Identifier: 75676352

GI number: 75676352

Start: 2364911

End: 2366218

Strand: Direct

Name: purB [H]

Synonym: Nwi_2167

Alternate gene names: 75676352

Gene position: 2364911-2366218 (Clockwise)

Preceding gene: 75676349

Following gene: 75676353

Centisome position: 69.51

GC content: 63.15

Gene sequence:

>1308_bases
ATGATCCCCCGTTACGCCCGCCCGGAAATGGTTTCGATCTGGGAGCCGCAGACCCGCTTCAAGATCTGGTTCGAGATCGA
GGCTCACGCCGCTGATGCCCAGGCCGAACTCGGTGTGATTCCGAAGGAAGCGGCCGAGATTATCTGGGCAAAGGCGAAAG
ACGCCACTTTCGATATTCAGCGCATTGACGAGATCGAGCGCGAAACCAGGCACGATGTCATCGCCTTCCTCACTCACCTC
GCCGAAATCGTCGGTCCCGAGGCGCGCTTCGTGCATCAGGGCATGACGTCATCGGACGTGCTCGACACCTGCCTCAACGT
ACAGCTCAGCCGGGCCGCCGACATCCTGATCGCCGATATCGACAAGCTGCTCGGGGCGCTGAAGACCCGCGCGTTCGAAC
ACAAGATGACGCCGACCATCGGCCGCTCGCACGGCATTCACGCCGAACCCGTGACCTTCGGCCTCAAGCTCGCTTACGCC
TATGCGGAGTTCTCGCGCGCCAGGGAGCGCCTGACCGCGGCCCGCCGGGAGGTCGCGACCTGCGCGATCTCGGGCGCGGT
CGGCACCTTCGCCCAGATCGATCCGCGCGTGGAAGAGCATGTCGCGGGCGCGATGGGATTGATCCCCGAGCCGGTCTCAA
CCCAGGTGATCCCGCGTGACCGCCACGCGATGTTCTTCGCGACGCTCGGCGTCATCGCGTCGTCGATGGAGCGGCTTGCC
ATCGAAATCCGCCATCTGCAACGCACCGAGGTGCTGGAAGCGGAGGAGTTCTTCTCCGAAGGCCAGAAAGGCTCGTCGGC
GATGCCGCACAAGCGCAACCCGGTTCTGACCGAGAACATCACGGGGCTGGCGCGTATGGTGCGCGCCTATGTGACGCCGG
CTCTGGAGAACGTCGCGCTCTGGCATGAACGTGATATCTCGCACTCCTCGGTCGAACGGATGATCGGCCCGGACGCCACC
GTGACGCTCGACTTCGCGCTCAACCGCCTCGCGGGCGTGATCGACAAGCTTCTGGTCTATCCCGCCAACATGCAAAAGAA
CCTCGACCGTCTCGGAGGACTCGTGCACTCGCAACGCGTGCTGATCGCGCTCACGCAAAAAGGCGCGAGCCGCGAGGACG
CCTACAAGCTGGTTCAGCGGAACGCCATGCCGGTGTGGCGCGGCGAAGGCGACTTCGAAACGCTTCTGAAGAACGATGCC
GACGTGACGAAATATCTCACCGGCGCCGAGATCGCCGAGCAGTTCGACCTCAACTATCACCTCAAGCACGTTGACACGAT
CTTCAAGCGGGTGTTCGGAAGCGCTTGA

Upstream 100 bases:

>100_bases
AGGCCTATGTCCGCTCGACAACCTTGCGTCGTTTGTCTGCTTACGGCGGCTCTGCTAGAGCGTGAGCTTGAAAAGCCTCC
GAAAAGCTCAAGGACCGTTC

Downstream 100 bases:

>100_bases
GCGGGCAGCCATCACCGATTTGGATGTCGACGCCGTTTCTTCACGCGAGCCGCCATCCAATCGGGGGAGGCCCGACGACA
GGCTTTCCTCGAAAACGCTA

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase; Glutamyl--tRNA ligase regulatory factor [H]

Number of amino acids: Translated: 435; Mature: 435

Protein sequence:

>435_residues
MIPRYARPEMVSIWEPQTRFKIWFEIEAHAADAQAELGVIPKEAAEIIWAKAKDATFDIQRIDEIERETRHDVIAFLTHL
AEIVGPEARFVHQGMTSSDVLDTCLNVQLSRAADILIADIDKLLGALKTRAFEHKMTPTIGRSHGIHAEPVTFGLKLAYA
YAEFSRARERLTAARREVATCAISGAVGTFAQIDPRVEEHVAGAMGLIPEPVSTQVIPRDRHAMFFATLGVIASSMERLA
IEIRHLQRTEVLEAEEFFSEGQKGSSAMPHKRNPVLTENITGLARMVRAYVTPALENVALWHERDISHSSVERMIGPDAT
VTLDFALNRLAGVIDKLLVYPANMQKNLDRLGGLVHSQRVLIALTQKGASREDAYKLVQRNAMPVWRGEGDFETLLKNDA
DVTKYLTGAEIAEQFDLNYHLKHVDTIFKRVFGSA

Sequences:

>Translated_435_residues
MIPRYARPEMVSIWEPQTRFKIWFEIEAHAADAQAELGVIPKEAAEIIWAKAKDATFDIQRIDEIERETRHDVIAFLTHL
AEIVGPEARFVHQGMTSSDVLDTCLNVQLSRAADILIADIDKLLGALKTRAFEHKMTPTIGRSHGIHAEPVTFGLKLAYA
YAEFSRARERLTAARREVATCAISGAVGTFAQIDPRVEEHVAGAMGLIPEPVSTQVIPRDRHAMFFATLGVIASSMERLA
IEIRHLQRTEVLEAEEFFSEGQKGSSAMPHKRNPVLTENITGLARMVRAYVTPALENVALWHERDISHSSVERMIGPDAT
VTLDFALNRLAGVIDKLLVYPANMQKNLDRLGGLVHSQRVLIALTQKGASREDAYKLVQRNAMPVWRGEGDFETLLKNDA
DVTKYLTGAEIAEQFDLNYHLKHVDTIFKRVFGSA
>Mature_435_residues
MIPRYARPEMVSIWEPQTRFKIWFEIEAHAADAQAELGVIPKEAAEIIWAKAKDATFDIQRIDEIERETRHDVIAFLTHL
AEIVGPEARFVHQGMTSSDVLDTCLNVQLSRAADILIADIDKLLGALKTRAFEHKMTPTIGRSHGIHAEPVTFGLKLAYA
YAEFSRARERLTAARREVATCAISGAVGTFAQIDPRVEEHVAGAMGLIPEPVSTQVIPRDRHAMFFATLGVIASSMERLA
IEIRHLQRTEVLEAEEFFSEGQKGSSAMPHKRNPVLTENITGLARMVRAYVTPALENVALWHERDISHSSVERMIGPDAT
VTLDFALNRLAGVIDKLLVYPANMQKNLDRLGGLVHSQRVLIALTQKGASREDAYKLVQRNAMPVWRGEGDFETLLKNDA
DVTKYLTGAEIAEQFDLNYHLKHVDTIFKRVFGSA

Specific function: Influences the affinity of glutamyl--tRNA ligase for its substrates and increases its thermostability [H]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Homo sapiens, GI4557269, Length=405, Percent_Identity=26.4197530864197, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI183227688, Length=415, Percent_Identity=25.5421686746988, Blast_Score=139, Evalue=4e-33,
Organism=Escherichia coli, GI1787376, Length=345, Percent_Identity=29.8550724637681, Blast_Score=89, Evalue=6e-19,
Organism=Caenorhabditis elegans, GI17508577, Length=394, Percent_Identity=25.8883248730964, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI32564234, Length=360, Percent_Identity=26.9444444444444, Blast_Score=93, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323391, Length=404, Percent_Identity=27.7227722772277, Blast_Score=138, Evalue=2e-33,
Organism=Drosophila melanogaster, GI24647570, Length=381, Percent_Identity=25.9842519685039, Blast_Score=106, Evalue=3e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019468
- InterPro:   IPR003031
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF10397 ADSL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 48593; Mature: 48593

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIPRYARPEMVSIWEPQTRFKIWFEIEAHAADAQAELGVIPKEAAEIIWAKAKDATFDIQ
CCCCCCCCCCEEECCCCCEEEEEEEEEECCCCCHHHCCCCCHHHHHHHHHCCCCCEEHHH
RIDEIERETRHDVIAFLTHLAEIVGPEARFVHQGMTSSDVLDTCLNVQLSRAADILIADI
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHH
DKLLGALKTRAFEHKMTPTIGRSHGIHAEPVTFGLKLAYAYAEFSRARERLTAARREVAT
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CAISGAVGTFAQIDPRVEEHVAGAMGLIPEPVSTQVIPRDRHAMFFATLGVIASSMERLA
HHHHCCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
IEIRHLQRTEVLEAEEFFSEGQKGSSAMPHKRNPVLTENITGLARMVRAYVTPALENVAL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
WHERDISHSSVERMIGPDATVTLDFALNRLAGVIDKLLVYPANMQKNLDRLGGLVHSQRV
HHHCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEE
LIALTQKGASREDAYKLVQRNAMPVWRGEGDFETLLKNDADVTKYLTGAEIAEQFDLNYH
EEEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCHH
LKHVDTIFKRVFGSA
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MIPRYARPEMVSIWEPQTRFKIWFEIEAHAADAQAELGVIPKEAAEIIWAKAKDATFDIQ
CCCCCCCCCCEEECCCCCEEEEEEEEEECCCCCHHHCCCCCHHHHHHHHHCCCCCEEHHH
RIDEIERETRHDVIAFLTHLAEIVGPEARFVHQGMTSSDVLDTCLNVQLSRAADILIADI
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHH
DKLLGALKTRAFEHKMTPTIGRSHGIHAEPVTFGLKLAYAYAEFSRARERLTAARREVAT
HHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
CAISGAVGTFAQIDPRVEEHVAGAMGLIPEPVSTQVIPRDRHAMFFATLGVIASSMERLA
HHHHCCHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
IEIRHLQRTEVLEAEEFFSEGQKGSSAMPHKRNPVLTENITGLARMVRAYVTPALENVAL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
WHERDISHSSVERMIGPDATVTLDFALNRLAGVIDKLLVYPANMQKNLDRLGGLVHSQRV
HHHCCCCHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCEE
LIALTQKGASREDAYKLVQRNAMPVWRGEGDFETLLKNDADVTKYLTGAEIAEQFDLNYH
EEEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHCCCHH
LKHVDTIFKRVFGSA
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3036807; 9384377; 1608947; 10926519 [H]