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The map label for this gene is hsrA [H]

Identifier: 75676333

GI number: 75676333

Start: 2345363

End: 2346835

Strand: Direct

Name: hsrA [H]

Synonym: Nwi_2148

Alternate gene names: 75676333

Gene position: 2345363-2346835 (Clockwise)

Preceding gene: 75676326

Following gene: 75676334

Centisome position: 68.94

GC content: 63.34

Gene sequence:

>1473_bases
ATGAACAAGGAACGCCTGGTCCCGCTGATCGTCGCCGCCGCCCTGTTCATGGAGAACATGGACTCCACGGTGATCGCGAC
ATCGTTGCCGGCGATCGCCGCCGATATCGGCGCCAGCCCGCTGACGCTGAAGCTCGCGATCACGTCCTACCTGTTGTCGC
TGGCGGTGTTCATACCGGCAAGCGGCTGGACCGCGGACCGGTTCGGGGCGCGCTCCGTCTTCAGCGCGGCCATCGTGGTT
TTCATGATCGGCTCGATCGGCTGCGCGCTATCATCATCGGTGACCGATTTCGTGATAGCGCGGACCCTGCAAGGGGTGGG
CGGCGCGATGATGACGCCGGTCGGCCGTCTGGTGCTGCTGCGTTCGATCGACAAGAGCGCGCTCGTCAACGCGATGGCGT
GGGTGACGGTACCGGCCTTGATCGGACCGGTGGTCGGTCCGCCGCTCGGCGGCTTCATCACCACCTATTTTTCATGGCAC
TGGATTTTTCTGATCAACATTCCGGTCGGCCTGATCGGCATCTTCATGGCGCAGCGCTACATCGACCCGATCCGCAGCGA
CAATCCCGAACGCTTCGACCTCTACGGGATGGTACTGGCTGGCATAGGGGTCGGCGGCATCGCCTTCGGCCTTTCGGTCG
CCGGTCTCAACCTCTTGCCGTGGACGGTGGTGACCGCGCTGATCGGAGCCGGGTCGGTCTCGATGGCGCTTTATGTGATG
CACGCCCGCCGCACCGACCAGCCGGTGCTCGACCTGACGCTGCTGCGCCTGCCGACGTTCCGGGCCAGCATCCATGGCGG
ATTTCTGTTTCGTGTCGGCGTCGGCGCGATGCCGTTCCTGCTGCCGCTTCTGATGCAGGTCGGCTTCGGCCTGTCGCCGT
TCGGCTCCGGGCTCGTGACATTCTCATCGGCCGTCGGCGCCATCGCCATGAAAACGCTGGCGGCCCGCATCATCCGCGCC
TTTGGCTTCCGCAACATCATGACTGTCAACGCGGTGGTGAGTTCGATCTTCCTCGCGGCCTGCGCGCTGTTCACGGTTAC
GACGCCGCTGCTGCTGATCATGATCATTCTTGTTGTCGGCGGCTTCTTTCGCTCGCTCCAGTTCACCGCGATCAACGCCC
TGACCTATGCCGATGTCGAGCCCGAGCGGATGAGCCGTGCGACCACGCTGGCCAGCGTCAATCAGCAACTGGCGATTTCA
GCAGGGGTCGCTCTCGGCGCATTCTCGGTCGAGACCACGATGCTGTTCCGCCACGGCACGGAATTGACAGCCGGCGATTT
CGGCTCGGGATTTCTCGTGGTCTCGCTGCTTTCCGCTATGTCCACTTTCTACTTCATGCGACTGTCGGCCGATGCCGGTC
ATCAGGTTTCCGGCCGGCGCATGGTCATCATTTCCGATCCTACCCGGGAATCCGAGGCTGAGGAGGAAGCGTCCCGCGAG
ACCGTTACGGCGCGGGATCAGCGGATAAGCTAA

Upstream 100 bases:

>100_bases
TCACCAAGGATTTAGGAAGGCTCACGCGCTGCGCAATAGCGCCACGGCCCAAATTGATGCTACAGCCCCGCTCCGCCGTC
CGCTTGCTCCGCGAGCCCGT

Downstream 100 bases:

>100_bases
GCTTTTACCTCAAGATCGCAGGAGGATTCCACATGGAGCCATTCTCGGGAGCGGTGGTGAATACCTACGCTGAAGGCCCA
CCTCGTCAGGTGCCGGGATT

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: High-copy suppressor of rspA [H]

Number of amino acids: Translated: 490; Mature: 490

Protein sequence:

>490_residues
MNKERLVPLIVAAALFMENMDSTVIATSLPAIAADIGASPLTLKLAITSYLLSLAVFIPASGWTADRFGARSVFSAAIVV
FMIGSIGCALSSSVTDFVIARTLQGVGGAMMTPVGRLVLLRSIDKSALVNAMAWVTVPALIGPVVGPPLGGFITTYFSWH
WIFLINIPVGLIGIFMAQRYIDPIRSDNPERFDLYGMVLAGIGVGGIAFGLSVAGLNLLPWTVVTALIGAGSVSMALYVM
HARRTDQPVLDLTLLRLPTFRASIHGGFLFRVGVGAMPFLLPLLMQVGFGLSPFGSGLVTFSSAVGAIAMKTLAARIIRA
FGFRNIMTVNAVVSSIFLAACALFTVTTPLLLIMIILVVGGFFRSLQFTAINALTYADVEPERMSRATTLASVNQQLAIS
AGVALGAFSVETTMLFRHGTELTAGDFGSGFLVVSLLSAMSTFYFMRLSADAGHQVSGRRMVIISDPTRESEAEEEASRE
TVTARDQRIS

Sequences:

>Translated_490_residues
MNKERLVPLIVAAALFMENMDSTVIATSLPAIAADIGASPLTLKLAITSYLLSLAVFIPASGWTADRFGARSVFSAAIVV
FMIGSIGCALSSSVTDFVIARTLQGVGGAMMTPVGRLVLLRSIDKSALVNAMAWVTVPALIGPVVGPPLGGFITTYFSWH
WIFLINIPVGLIGIFMAQRYIDPIRSDNPERFDLYGMVLAGIGVGGIAFGLSVAGLNLLPWTVVTALIGAGSVSMALYVM
HARRTDQPVLDLTLLRLPTFRASIHGGFLFRVGVGAMPFLLPLLMQVGFGLSPFGSGLVTFSSAVGAIAMKTLAARIIRA
FGFRNIMTVNAVVSSIFLAACALFTVTTPLLLIMIILVVGGFFRSLQFTAINALTYADVEPERMSRATTLASVNQQLAIS
AGVALGAFSVETTMLFRHGTELTAGDFGSGFLVVSLLSAMSTFYFMRLSADAGHQVSGRRMVIISDPTRESEAEEEASRE
TVTARDQRIS
>Mature_490_residues
MNKERLVPLIVAAALFMENMDSTVIATSLPAIAADIGASPLTLKLAITSYLLSLAVFIPASGWTADRFGARSVFSAAIVV
FMIGSIGCALSSSVTDFVIARTLQGVGGAMMTPVGRLVLLRSIDKSALVNAMAWVTVPALIGPVVGPPLGGFITTYFSWH
WIFLINIPVGLIGIFMAQRYIDPIRSDNPERFDLYGMVLAGIGVGGIAFGLSVAGLNLLPWTVVTALIGAGSVSMALYVM
HARRTDQPVLDLTLLRLPTFRASIHGGFLFRVGVGAMPFLLPLLMQVGFGLSPFGSGLVTFSSAVGAIAMKTLAARIIRA
FGFRNIMTVNAVVSSIFLAACALFTVTTPLLLIMIILVVGGFFRSLQFTAINALTYADVEPERMSRATTLASVNQQLAIS
AGVALGAFSVETTMLFRHGTELTAGDFGSGFLVVSLLSAMSTFYFMRLSADAGHQVSGRRMVIISDPTRESEAEEEASRE
TVTARDQRIS

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1790195, Length=470, Percent_Identity=37.2340425531915, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI1788392, Length=461, Percent_Identity=37.3101952277657, Blast_Score=268, Evalue=9e-73,
Organism=Escherichia coli, GI1789042, Length=440, Percent_Identity=25.9090909090909, Blast_Score=128, Evalue=8e-31,
Organism=Escherichia coli, GI1788710, Length=409, Percent_Identity=26.4058679706601, Blast_Score=118, Evalue=8e-28,
Organism=Escherichia coli, GI87081983, Length=310, Percent_Identity=28.3870967741935, Blast_Score=109, Evalue=4e-25,
Organism=Escherichia coli, GI1790146, Length=169, Percent_Identity=30.7692307692308, Blast_Score=72, Evalue=8e-14,
Organism=Saccharomyces cerevisiae, GI6325455, Length=232, Percent_Identity=24.1379310344828, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322958, Length=162, Percent_Identity=27.7777777777778, Blast_Score=63, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR001411 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 52024; Mature: 52024

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKERLVPLIVAAALFMENMDSTVIATSLPAIAADIGASPLTLKLAITSYLLSLAVFIPA
CCCCHHHHHHHHHHHHHHCCCCEEEHHHCHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCC
SGWTADRFGARSVFSAAIVVFMIGSIGCALSSSVTDFVIARTLQGVGGAMMTPVGRLVLL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
RSIDKSALVNAMAWVTVPALIGPVVGPPLGGFITTYFSWHWIFLINIPVGLIGIFMAQRY
HHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEEEEECHHHHHHHHHHHHH
IDPIRSDNPERFDLYGMVLAGIGVGGIAFGLSVAGLNLLPWTVVTALIGAGSVSMALYVM
HHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHH
HARRTDQPVLDLTLLRLPTFRASIHGGFLFRVGVGAMPFLLPLLMQVGFGLSPFGSGLVT
HHCCCCCCHHHHHHHHCCHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHH
FSSAVGAIAMKTLAARIIRAFGFRNIMTVNAVVSSIFLAACALFTVTTPLLLIMIILVVG
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFFRSLQFTAINALTYADVEPERMSRATTLASVNQQLAISAGVALGAFSVETTMLFRHGT
HHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCC
ELTAGDFGSGFLVVSLLSAMSTFYFMRLSADAGHQVSGRRMVIISDPTRESEAEEEASRE
CCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHH
TVTARDQRIS
HHHHHHCCCC
>Mature Secondary Structure
MNKERLVPLIVAAALFMENMDSTVIATSLPAIAADIGASPLTLKLAITSYLLSLAVFIPA
CCCCHHHHHHHHHHHHHHCCCCEEEHHHCHHHHHHCCCCCEEEHHHHHHHHHHHHHHHCC
SGWTADRFGARSVFSAAIVVFMIGSIGCALSSSVTDFVIARTLQGVGGAMMTPVGRLVLL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
RSIDKSALVNAMAWVTVPALIGPVVGPPLGGFITTYFSWHWIFLINIPVGLIGIFMAQRY
HHCCHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHEEEEEEECHHHHHHHHHHHHH
IDPIRSDNPERFDLYGMVLAGIGVGGIAFGLSVAGLNLLPWTVVTALIGAGSVSMALYVM
HHHHCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCHHHHHHHHHHHCCCHHHHHHHHH
HARRTDQPVLDLTLLRLPTFRASIHGGFLFRVGVGAMPFLLPLLMQVGFGLSPFGSGLVT
HHCCCCCCHHHHHHHHCCHHHHHHCCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCHHHH
FSSAVGAIAMKTLAARIIRAFGFRNIMTVNAVVSSIFLAACALFTVTTPLLLIMIILVVG
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GFFRSLQFTAINALTYADVEPERMSRATTLASVNQQLAISAGVALGAFSVETTMLFRHGT
HHHHHHHHHHHHEEEECCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCC
ELTAGDFGSGFLVVSLLSAMSTFYFMRLSADAGHQVSGRRMVIISDPTRESEAEEEASRE
CCCCCCCCCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCEEEEEECCCCCCHHHHHHHHH
TVTARDQRIS
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7686882; 9278503 [H]