BacMap

Definition	Nitrobacter winogradskyi Nb-255, complete genome.
Accession	NC_007406
Length	3,402,093

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The map label for this gene is yciK [H]

Identifier: 75676209

GI number: 75676209

Start: 2209690

End: 2210442

Strand: Direct

Name: yciK [H]

Synonym: Nwi_2019

Alternate gene names: 75676209

Gene position: 2209690-2210442 (Clockwise)

Preceding gene: 75676207

Following gene: 75676216

Centisome position: 64.95

GC content: 65.6

Gene sequence:

>753_bases
ATGACAAAACCGCTCACTTCCCGCGTCGCGCTCGTGACCGGAGCTTCGCGCGGAATCGGCTACGCCACGGCGCGCGCGCT
TGCAAAAGCCGGCGCGCATGTGATCGCCGTGGCTCGCACGCAGGGCGGACTCGAAGAGCTTGACGACGACATCCGCGACG
GCGGCGGCAGCGCGACGCTGGTGCCGCTCAATCTCTCCGACTTCGACGGGATCGCCCGGCTCGGCGCCGCATTGAACGAG
CGCCACGGCAAGCTCGATATTCTCGTCGGCAACGCCGGTATCGCCGGTCCGTCCTCGCCGCTCGGCCACATCGAGATGAA
ACCATGGCAGGACGTGATGGCGATCAATGTCACCGCGAATTTTCAGTTGATCCGCTGCATGGAGCCGTTGCTGCGGCAGT
CGGACGCCGGACGCGCGGTGTTCGTCACCTCCGGCGCCGCCGGCAAGGCCAACGCCTATCGCGGGCCCTACGCGGCCTCG
AAGGCCGCGCTGGATACGCTGGTGCGATGCTGGGCGAGCGAAACCGCGACGACCGGAATGCGGGTCAACCTGTTCGATCC
CGGGCCGGTCCGGACCCGGCTGCGCGCCATCGTGTTTCCGGGCGAGGATCCCATGACGCTGGACACACCCGAACAAATTG
CCGAACTCATCATCCCGATGTGCCTGCCCTCCTGGAGCGAGACCGGAAAACTGTACGACTACAAGAGCCGAAGACTGACA
GACTCTCGCGACCGACCGGTTTCGAACCTCTAA

Upstream 100 bases:

>100_bases
TGACGACATCGGCCGCCATTGGCCTTTGGCCTCGGCGGATAATTCAGGCATAAGGCGCAAACCTCCGTTCTCGTCACCCC
TAGCACGAAGCACCCCTGTC

Downstream 100 bases:

>100_bases
CAAGAGTTCGGGAACCTTCGCGGAGATATTATCGTTTCCGAGTGATGTGGATACCGGCGCGCGCGAAAAACGCGTCGAAA
CAACATCTGGAGTTCGCTGT

Product: short-chain dehydrogenase/reductase SDR

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 250; Mature: 249

Protein sequence:

>250_residues
MTKPLTSRVALVTGASRGIGYATARALAKAGAHVIAVARTQGGLEELDDDIRDGGGSATLVPLNLSDFDGIARLGAALNE
RHGKLDILVGNAGIAGPSSPLGHIEMKPWQDVMAINVTANFQLIRCMEPLLRQSDAGRAVFVTSGAAGKANAYRGPYAAS
KAALDTLVRCWASETATTGMRVNLFDPGPVRTRLRAIVFPGEDPMTLDTPEQIAELIIPMCLPSWSETGKLYDYKSRRLT
DSRDRPVSNL

Sequences:

>Translated_250_residues
MTKPLTSRVALVTGASRGIGYATARALAKAGAHVIAVARTQGGLEELDDDIRDGGGSATLVPLNLSDFDGIARLGAALNE
RHGKLDILVGNAGIAGPSSPLGHIEMKPWQDVMAINVTANFQLIRCMEPLLRQSDAGRAVFVTSGAAGKANAYRGPYAAS
KAALDTLVRCWASETATTGMRVNLFDPGPVRTRLRAIVFPGEDPMTLDTPEQIAELIIPMCLPSWSETGKLYDYKSRRLT
DSRDRPVSNL
>Mature_249_residues
TKPLTSRVALVTGASRGIGYATARALAKAGAHVIAVARTQGGLEELDDDIRDGGGSATLVPLNLSDFDGIARLGAALNER
HGKLDILVGNAGIAGPSSPLGHIEMKPWQDVMAINVTANFQLIRCMEPLLRQSDAGRAVFVTSGAAGKANAYRGPYAASK
AALDTLVRCWASETATTGMRVNLFDPGPVRTRLRAIVFPGEDPMTLDTPEQIAELIIPMCLPSWSETGKLYDYKSRRLTD
SRDRPVSNL

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=228, Percent_Identity=30.7017543859649, Blast_Score=98, Evalue=7e-21,
Organism=Homo sapiens, GI109715829, Length=230, Percent_Identity=32.1739130434783, Blast_Score=92, Evalue=6e-19,
Organism=Homo sapiens, GI126723191, Length=182, Percent_Identity=33.5164835164835, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI33667109, Length=206, Percent_Identity=33.0097087378641, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI5031737, Length=210, Percent_Identity=32.3809523809524, Blast_Score=85, Evalue=7e-17,
Organism=Homo sapiens, GI7657478, Length=212, Percent_Identity=30.188679245283, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI4504505, Length=248, Percent_Identity=27.4193548387097, Blast_Score=77, Evalue=1e-14,
Organism=Homo sapiens, GI126723750, Length=199, Percent_Identity=30.1507537688442, Blast_Score=76, Evalue=2e-14,
Organism=Homo sapiens, GI20149619, Length=213, Percent_Identity=27.6995305164319, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI7705925, Length=192, Percent_Identity=32.2916666666667, Blast_Score=72, Evalue=4e-13,
Organism=Homo sapiens, GI32455239, Length=181, Percent_Identity=32.0441988950276, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI5031765, Length=181, Percent_Identity=32.0441988950276, Blast_Score=71, Evalue=8e-13,
Organism=Homo sapiens, GI7706318, Length=208, Percent_Identity=28.3653846153846, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI19923817, Length=195, Percent_Identity=26.6666666666667, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI59889578, Length=172, Percent_Identity=26.7441860465116, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787526, Length=238, Percent_Identity=35.7142857142857, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1787335, Length=187, Percent_Identity=32.620320855615, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI2367175, Length=209, Percent_Identity=33.4928229665072, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1790717, Length=190, Percent_Identity=29.4736842105263, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1789378, Length=200, Percent_Identity=36, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI87082100, Length=218, Percent_Identity=30.2752293577982, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787905, Length=232, Percent_Identity=31.0344827586207, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI1786812, Length=196, Percent_Identity=33.6734693877551, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87082160, Length=192, Percent_Identity=27.6041666666667, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1787820, Length=218, Percent_Identity=26.605504587156, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786701, Length=195, Percent_Identity=29.7435897435897, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1789208, Length=198, Percent_Identity=29.7979797979798, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788459, Length=225, Percent_Identity=25.3333333333333, Blast_Score=62, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17560676, Length=205, Percent_Identity=33.1707317073171, Blast_Score=93, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI17562910, Length=206, Percent_Identity=33.9805825242718, Blast_Score=92, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17551412, Length=203, Percent_Identity=33.9901477832512, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17538480, Length=199, Percent_Identity=34.1708542713568, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17538486, Length=229, Percent_Identity=31.0043668122271, Blast_Score=85, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17561402, Length=195, Percent_Identity=27.6923076923077, Blast_Score=84, Evalue=5e-17,
Organism=Caenorhabditis elegans, GI72000259, Length=229, Percent_Identity=32.7510917030568, Blast_Score=84, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI17563726, Length=237, Percent_Identity=30.8016877637131, Blast_Score=82, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17560332, Length=200, Percent_Identity=32, Blast_Score=81, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17562908, Length=202, Percent_Identity=35.6435643564356, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17568967, Length=266, Percent_Identity=24.812030075188, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI115534694, Length=191, Percent_Identity=32.9842931937173, Blast_Score=78, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17531453, Length=197, Percent_Identity=36.0406091370558, Blast_Score=77, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17562906, Length=201, Percent_Identity=35.3233830845771, Blast_Score=76, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17562904, Length=195, Percent_Identity=32.8205128205128, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17555706, Length=220, Percent_Identity=28.6363636363636, Blast_Score=74, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI17560150, Length=196, Percent_Identity=31.6326530612245, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17565030, Length=199, Percent_Identity=34.6733668341709, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI17508895, Length=196, Percent_Identity=30.1020408163265, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17562990, Length=198, Percent_Identity=31.8181818181818, Blast_Score=70, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI71994604, Length=211, Percent_Identity=29.3838862559242, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI17560220, Length=205, Percent_Identity=32.6829268292683, Blast_Score=70, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI193204405, Length=206, Percent_Identity=33.0097087378641, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17559104, Length=199, Percent_Identity=34.1708542713568, Blast_Score=69, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71994600, Length=201, Percent_Identity=29.3532338308458, Blast_Score=67, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17564282, Length=159, Percent_Identity=29.559748427673, Blast_Score=67, Evalue=9e-12,
Organism=Caenorhabditis elegans, GI17555612, Length=216, Percent_Identity=29.1666666666667, Blast_Score=64, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17538182, Length=237, Percent_Identity=25.7383966244726, Blast_Score=64, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17536025, Length=223, Percent_Identity=30.9417040358744, Blast_Score=64, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6322227, Length=195, Percent_Identity=31.7948717948718, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323882, Length=204, Percent_Identity=30.8823529411765, Blast_Score=76, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6322226, Length=169, Percent_Identity=31.3609467455621, Blast_Score=72, Evalue=6e-14,
Organism=Saccharomyces cerevisiae, GI6322861, Length=226, Percent_Identity=28.3185840707965, Blast_Score=70, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21355319, Length=225, Percent_Identity=30.6666666666667, Blast_Score=98, Evalue=6e-21,
Organism=Drosophila melanogaster, GI21357041, Length=192, Percent_Identity=35.4166666666667, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28571526, Length=189, Percent_Identity=33.3333333333333, Blast_Score=86, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21358495, Length=234, Percent_Identity=27.7777777777778, Blast_Score=84, Evalue=8e-17,
Organism=Drosophila melanogaster, GI23397609, Length=192, Percent_Identity=34.375, Blast_Score=83, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24644339, Length=195, Percent_Identity=32.3076923076923, Blast_Score=74, Evalue=7e-14,
Organism=Drosophila melanogaster, GI24639444, Length=233, Percent_Identity=30.0429184549356, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24584752, Length=190, Percent_Identity=30.5263157894737, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 26629; Mature: 26498

Theoretical pI: Translated: 8.47; Mature: 8.47

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKPLTSRVALVTGASRGIGYATARALAKAGAHVIAVARTQGGLEELDDDIRDGGGSATL
CCCCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCCCEEE
VPLNLSDFDGIARLGAALNERHGKLDILVGNAGIAGPSSPLGHIEMKPWQDVMAINVTAN
EEECCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCEECCCCCCEEEEEEECC
FQLIRCMEPLLRQSDAGRAVFVTSGAAGKANAYRGPYAASKAALDTLVRCWASETATTGM
CHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCE
RVNLFDPGPVRTRLRAIVFPGEDPMTLDTPEQIAELIIPMCLPSWSETGKLYDYKSRRLT
EEEEECCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCCC
DSRDRPVSNL
CCCCCCCCCC
>Mature Secondary Structure 
TKPLTSRVALVTGASRGIGYATARALAKAGAHVIAVARTQGGLEELDDDIRDGGGSATL
CCCCCCCEEEEECCCCCCHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHCCCCCEEE
VPLNLSDFDGIARLGAALNERHGKLDILVGNAGIAGPSSPLGHIEMKPWQDVMAINVTAN
EEECCCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCEECCCCCCEEEEEEECC
FQLIRCMEPLLRQSDAGRAVFVTSGAAGKANAYRGPYAASKAALDTLVRCWASETATTGM
CHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCE
RVNLFDPGPVRTRLRAIVFPGEDPMTLDTPEQIAELIIPMCLPSWSETGKLYDYKSRRLT
EEEEECCCCCCEEEEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCCC
DSRDRPVSNL
CCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Oxidoreductases; Acting on the CH-OH group of donors; With NAD or NADP as acceptor [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1885549 [H]