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The map label for this gene is purF [H]

Identifier: 75676207

GI number: 75676207

Start: 2207564

End: 2209078

Strand: Direct

Name: purF [H]

Synonym: Nwi_2017

Alternate gene names: 75676207

Gene position: 2207564-2209078 (Clockwise)

Preceding gene: 75676206

Following gene: 75676209

Centisome position: 64.89

GC content: 63.04

Gene sequence:

>1515_bases
ATGGATTGTCAGCAGAGCCTTTCCAGCGACGCCTCCCCCAACGATCCCGACACTGATTTTGATCTTGACGGCGACACGCT
GCGCGAGGAGTGCGGCGTGTTCGGCATTTTCGGCCATCCGGAAGCCGCGGCGATCACCGCGCTCGGCCTTCATGCTCTCC
AGCACCGCGGCCAGGAAGCAGCGGGAATCGTGACCTTCGACGGCCACCGCTTCCACTCCGAACGCCGGCTCGGGCTTGTC
GGCGACGCCTTCTCCCGCCGCGAGGTGATTGAACGTCTTCCCGGCAGCGCCGCTGTCGGCCACACCCGTTATTCCACCAC
CGGCGGCACCATCCTGCGCAACGTGCAGCCGCTGTTCGCAGAACTTCACGCCGGCGGCTTCGCCGTCGCCCACAACGGCA
ACCTCACCAACGGACTGACGCTGCGCCGCGAGCTGGTGGGCCGTGGCGCCTTGATGCAATCGACCTCCGATACCGAGGCC
ATCCTTCATCTGGTCGCGCAGTCGAGGCGCGGTCGTTTCATAGAGCGCTATATCGAAGCGCTCCGGGCCATTGAGGGCGC
TTATGCGCTGGTGTCGCTGACCAACAAGAAGCTGGTCGGAGCGCGCGATCCGCGAGGCATCCGTCCGCTCGTGCTCGGCG
AACTCGACGGTTGCCCGATCCTTGCCTCGGAAACCTGTGCGCTCGACATCATCGGCGCCAAGTATGTCCGTGACATCGAG
CCCGGCGAAGTCATCGTGTTTCACCGCAAGGATGACGTCATTCAAACCGAAATTCACAAGCCCTTTCCTCCGATGCCGCC
CAGGCCGTGCATCTTCGAGTATATCTATTTTTCACGGCCGGATTCGATCGTCGCAGGCCGTTCGGTCTATGAAGTCCGCA
AGGCGTTCGGCGCGCAACTGGCCCGCGAAAGCCATGTCGATGTCGATGTCGTCGTGCCGGTGCCCGATTCCGGCGTGCCG
GCCGCCCTTGGCTACAGTCGTTTCTCCGGGGTGCCCTACGAACTAGGCATCATCAGAAATCACTATGTCGGCCGCACATT
CATTCAGCCCGCCCAAAGCGTTCGCGAACTCGGCGTCCGGATGAAACATTCCGCCAACCGCGCCGCGATCGCCGGCAAAC
GCATCATCCTGATCGACGACTCGCTGGTGCGCGGCACGACTTCGAGAAAGATCGTCCGCATGATGCGCGATGCCGGGGCT
ACGGAGGTGCATTTCCGCCTCGCCTCGCCTCCGATCATTCATCCCGATTACTACGGCATCGACCTGCCCGACCGGAACGG
GCTGTTGGCCGCGACCCACAGCCTCGAAGAGATGCGTGAGATGATCGGCGCGGATTCGCTGGCCTTCCTCTCCATCGACG
GCATGTACCGCGCTATGGGCGAACCCGGCCGCGATCCCGCGCATCCGAAATACGCCGACCATTGCTTTACGGGCGCCTAT
CCGACCAGCCTTACCGACCATACCGACGTCGAACCGCAGCCTCATCAGTTGTCGTTGCTCGCGGAAGCCAGCTGA

Upstream 100 bases:

>100_bases
GCCTGCAAAAGCTGATCGACGGCAAACCGGCGGAACATTAGTTGGGAGCGCGGACTTGACGTTCACGTCTTACTCAGGTG
CCGCCGAAAAGCGAGGCGCA

Downstream 100 bases:

>100_bases
GCGGAAACGCTCCACGGACGACCTGCGGTCAAAGCTGCCGGACCATGAACGCCGCCGCTTTGCTGTAACCGCGCAGCTTC
CGCGCGAGCGTGGAATCGGC

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 504; Mature: 504

Protein sequence:

>504_residues
MDCQQSLSSDASPNDPDTDFDLDGDTLREECGVFGIFGHPEAAAITALGLHALQHRGQEAAGIVTFDGHRFHSERRLGLV
GDAFSRREVIERLPGSAAVGHTRYSTTGGTILRNVQPLFAELHAGGFAVAHNGNLTNGLTLRRELVGRGALMQSTSDTEA
ILHLVAQSRRGRFIERYIEALRAIEGAYALVSLTNKKLVGARDPRGIRPLVLGELDGCPILASETCALDIIGAKYVRDIE
PGEVIVFHRKDDVIQTEIHKPFPPMPPRPCIFEYIYFSRPDSIVAGRSVYEVRKAFGAQLARESHVDVDVVVPVPDSGVP
AALGYSRFSGVPYELGIIRNHYVGRTFIQPAQSVRELGVRMKHSANRAAIAGKRIILIDDSLVRGTTSRKIVRMMRDAGA
TEVHFRLASPPIIHPDYYGIDLPDRNGLLAATHSLEEMREMIGADSLAFLSIDGMYRAMGEPGRDPAHPKYADHCFTGAY
PTSLTDHTDVEPQPHQLSLLAEAS

Sequences:

>Translated_504_residues
MDCQQSLSSDASPNDPDTDFDLDGDTLREECGVFGIFGHPEAAAITALGLHALQHRGQEAAGIVTFDGHRFHSERRLGLV
GDAFSRREVIERLPGSAAVGHTRYSTTGGTILRNVQPLFAELHAGGFAVAHNGNLTNGLTLRRELVGRGALMQSTSDTEA
ILHLVAQSRRGRFIERYIEALRAIEGAYALVSLTNKKLVGARDPRGIRPLVLGELDGCPILASETCALDIIGAKYVRDIE
PGEVIVFHRKDDVIQTEIHKPFPPMPPRPCIFEYIYFSRPDSIVAGRSVYEVRKAFGAQLARESHVDVDVVVPVPDSGVP
AALGYSRFSGVPYELGIIRNHYVGRTFIQPAQSVRELGVRMKHSANRAAIAGKRIILIDDSLVRGTTSRKIVRMMRDAGA
TEVHFRLASPPIIHPDYYGIDLPDRNGLLAATHSLEEMREMIGADSLAFLSIDGMYRAMGEPGRDPAHPKYADHCFTGAY
PTSLTDHTDVEPQPHQLSLLAEAS
>Mature_504_residues
MDCQQSLSSDASPNDPDTDFDLDGDTLREECGVFGIFGHPEAAAITALGLHALQHRGQEAAGIVTFDGHRFHSERRLGLV
GDAFSRREVIERLPGSAAVGHTRYSTTGGTILRNVQPLFAELHAGGFAVAHNGNLTNGLTLRRELVGRGALMQSTSDTEA
ILHLVAQSRRGRFIERYIEALRAIEGAYALVSLTNKKLVGARDPRGIRPLVLGELDGCPILASETCALDIIGAKYVRDIE
PGEVIVFHRKDDVIQTEIHKPFPPMPPRPCIFEYIYFSRPDSIVAGRSVYEVRKAFGAQLARESHVDVDVVVPVPDSGVP
AALGYSRFSGVPYELGIIRNHYVGRTFIQPAQSVRELGVRMKHSANRAAIAGKRIILIDDSLVRGTTSRKIVRMMRDAGA
TEVHFRLASPPIIHPDYYGIDLPDRNGLLAATHSLEEMREMIGADSLAFLSIDGMYRAMGEPGRDPAHPKYADHCFTGAY
PTSLTDHTDVEPQPHQLSLLAEAS

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=474, Percent_Identity=38.8185654008439, Blast_Score=325, Evalue=5e-89,
Organism=Escherichia coli, GI1788651, Length=480, Percent_Identity=38.9583333333333, Blast_Score=305, Evalue=5e-84,
Organism=Escherichia coli, GI1790167, Length=223, Percent_Identity=30.0448430493274, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17554892, Length=482, Percent_Identity=36.5145228215768, Blast_Score=294, Evalue=7e-80,
Organism=Saccharomyces cerevisiae, GI6323958, Length=501, Percent_Identity=34.9301397205589, Blast_Score=268, Evalue=2e-72,
Organism=Drosophila melanogaster, GI24659598, Length=498, Percent_Identity=39.7590361445783, Blast_Score=340, Evalue=9e-94,
Organism=Drosophila melanogaster, GI28573187, Length=497, Percent_Identity=39.8390342052314, Blast_Score=337, Evalue=1e-92,
Organism=Drosophila melanogaster, GI24659604, Length=472, Percent_Identity=40.2542372881356, Blast_Score=334, Evalue=1e-91,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 55103; Mature: 55103

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDCQQSLSSDASPNDPDTDFDLDGDTLREECGVFGIFGHPEAAAITALGLHALQHRGQEA
CCCHHHHCCCCCCCCCCCCCCCCCHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHCCCCC
AGIVTFDGHRFHSERRLGLVGDAFSRREVIERLPGSAAVGHTRYSTTGGTILRNVQPLFA
CEEEEECCCEECCCCCCCCCCCHHHHHHHHHHCCCCCEECCCEECCCCCHHHHHHHHHHH
ELHAGGFAVAHNGNLTNGLTLRRELVGRGALMQSTSDTEAILHLVAQSRRGRFIERYIEA
HHCCCCEEEEECCCCCCCHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
LRAIEGAYALVSLTNKKLVGARDPRGIRPLVLGELDGCPILASETCALDIIGAKYVRDIE
HHHHHCCEEEEEECCCCEECCCCCCCCCCEEEECCCCCCEECCCCEEEEEECHHHHCCCC
PGEVIVFHRKDDVIQTEIHKPFPPMPPRPCIFEYIYFSRPDSIVAGRSVYEVRKAFGAQL
CCCEEEEECCCCCHHHHCCCCCCCCCCCCCEEHEEECCCCCCHHCCHHHHHHHHHHHHHH
ARESHVDVDVVVPVPDSGVPAALGYSRFSGVPYELGIIRNHYVGRTFIQPAQSVRELGVR
HHHCCCCEEEEEECCCCCCCHHHCHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
MKHSANRAAIAGKRIILIDDSLVRGTTSRKIVRMMRDAGATEVHFRLASPPIIHPDYYGI
HHCCCCCCEECCCEEEEECCHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCEECCCCCCC
DLPDRNGLLAATHSLEEMREMIGADSLAFLSIDGMYRAMGEPGRDPAHPKYADHCFTGAY
CCCCCCCEEEHHHHHHHHHHHHCCCCEEEEEECHHHHHCCCCCCCCCCCCHHHHCCCCCC
PTSLTDHTDVEPQPHQLSLLAEAS
CCCCCCCCCCCCCCCEEEEEEECC
>Mature Secondary Structure
MDCQQSLSSDASPNDPDTDFDLDGDTLREECGVFGIFGHPEAAAITALGLHALQHRGQEA
CCCHHHHCCCCCCCCCCCCCCCCCHHHHHHCCEEEECCCCCHHHHHHHHHHHHHHCCCCC
AGIVTFDGHRFHSERRLGLVGDAFSRREVIERLPGSAAVGHTRYSTTGGTILRNVQPLFA
CEEEEECCCEECCCCCCCCCCCHHHHHHHHHHCCCCCEECCCEECCCCCHHHHHHHHHHH
ELHAGGFAVAHNGNLTNGLTLRRELVGRGALMQSTSDTEAILHLVAQSRRGRFIERYIEA
HHCCCCEEEEECCCCCCCHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHCCHHHHHHHHH
LRAIEGAYALVSLTNKKLVGARDPRGIRPLVLGELDGCPILASETCALDIIGAKYVRDIE
HHHHHCCEEEEEECCCCEECCCCCCCCCCEEEECCCCCCEECCCCEEEEEECHHHHCCCC
PGEVIVFHRKDDVIQTEIHKPFPPMPPRPCIFEYIYFSRPDSIVAGRSVYEVRKAFGAQL
CCCEEEEECCCCCHHHHCCCCCCCCCCCCCEEHEEECCCCCCHHCCHHHHHHHHHHHHHH
ARESHVDVDVVVPVPDSGVPAALGYSRFSGVPYELGIIRNHYVGRTFIQPAQSVRELGVR
HHHCCCCEEEEEECCCCCCCHHHCHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHH
MKHSANRAAIAGKRIILIDDSLVRGTTSRKIVRMMRDAGATEVHFRLASPPIIHPDYYGI
HHCCCCCCEECCCEEEEECCHHHCCCHHHHHHHHHHHCCCCEEEEEECCCCEECCCCCCC
DLPDRNGLLAATHSLEEMREMIGADSLAFLSIDGMYRAMGEPGRDPAHPKYADHCFTGAY
CCCCCCCEEEHHHHHHHHHHHHCCCCEEEEEECHHHHHCCCCCCCCCCCCHHHHCCCCCC
PTSLTDHTDVEPQPHQLSLLAEAS
CCCCCCCCCCCCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA