Click here to switch to the map view.

The map label for this gene is dnaB [H]

Identifier: 75676203

GI number: 75676203

Start: 2202451

End: 2203941

Strand: Direct

Name: dnaB [H]

Synonym: Nwi_2013

Alternate gene names: 75676203

Gene position: 2202451-2203941 (Clockwise)

Preceding gene: 75676202

Following gene: 75676204

Centisome position: 64.74

GC content: 62.51

Gene sequence:

>1491_bases
ATGGCTGATTCGAACATCCTGAAGCTTGCGCCTGATGCGACGACGCCAGCCTATCGCACCCCGCCGCATAATCTCGAAGC
GGAGCGCGACCTTCTCGGCGCCATTCTCGTCAACAACGACGCATTCTACAGGGTGTCCGATTTCCTCGAGCCGAAGCACT
TCTTCTACCCGGTGCATCGGACCATCTTCGAGACCGCGGGCAGCCTGATCCGCAGCGGCAAGATCGCGACGCCCGTCACG
GTCAAGACCTTCATTCCCGCTGACACCGACATCGGCGGAATGACCGCAAGCCAGTACCTCGCCCGGCTGGCGGCGGAAGC
CACCACGATCATCAACGCCCTGCACTACGGACGCACGATCTATGAGCAGGCGCTGCGCCGCGACCTGATCCACATCGGCG
AGGACATGGTCAACGTCGCCTATGACGCGCCGGTCGATTTCGCGCCCAAGGCGCAGATTGAGGATGCCGAGCGCCGGCTC
TACGAACTCGCCGAATCCGGCGGCTATGAAGGCGGCTTCCAGAAATTTTCCCAGGCGATGAAGACCGCGCTCGACATGGC
GGCCAACGCCTATCGCCGCGACGGGAAGCTGTCCGGCATCGCGACGGGATTGCGTGACCTCGACTCGCGAATGGGAGGGT
TGCAGCCGTCCGATCTGATCATCGTGGCGGGACGGCCGGGCATGGGCAAGACGGCGCTCGCCACCAACATCGCCTACAAC
ATCGCCAAGGCCTATCAGGCGGAGCTTCAGCCCGACGGCACGATGAAGACCGTCAATGGCGGGATCGTTGGCTTCTTCTC
CTGCGAAATGTCGGCCGAGCAACTGGCGACGCGTATTCTCGCCGAGCAGACCAGCATCGCTTCCAGCAATATCCGACGCG
GCGGCATCACCGAAAGCGACTTCGAGAAGATCCGCGATCACACCATCGAGCTGGAGCACCTGCCGCTCTTCGTCGACGAG
ACCGGCGGCCTGTCGATTTCGCAGTTGACGGCGCGCGCCCGCCGCCTGAAGCGCCAGAAGGGGCTCGACGTGCTGATCGT
CGACTACATCCAGCTTCTGCTGGGCAGCGGCAAGCGCAGCGACAACCGCGTGCAGGAAGTCACCGAGATCACAACCAGCC
TGAAAGCATTGGCGAAAGAACTGAACGTTCCGATCCTCGCGCTGTCGCAACTGTCGCGTCAGGTCGAAAGCCGTGACGAC
AAGCGCCCGCAACTTTCAGACCTTCGCGAATCCGGTTCGATCGAGCAGGACGCCGATGTCGTGATGTTCGTCTTCCGGGA
GGAATACTATCTCCAGAACAAGGAGCCGCGCGCGGGCACGCCGGAGCACGAGAAATGGCAGCTCGATATGTCGCTGGTGC
ACGGCAAGGCCGAAGTCATCATCGGCAAGCAGCGTCACGGGCCGACCGGCACCGTCGAACTGCAGTTCGAGGGACAGTTC
ACGCGCTTCAGCGATCTCGCACATGAAGGCCACCTGCCGGAGCGGATCTAG

Upstream 100 bases:

>100_bases
TTTATGCGGGGCCCATATCCCGCAAATATAGCGGGGCCGTATCCCGCAAAAATAGCGGGGCTCGTATCCCGCAAATGATA
AAGGTCAGAATCCGAAAGCC

Downstream 100 bases:

>100_bases
CTTACAGACCTTCCAGGCAGACCGCTCGCGCGGTTGAACCGCCGTGCAGCCGGGCGTAGCATGTAGCATGAATCTCGTGC
CCGATTCTAAAGTGAGCGGC

Product: replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 496; Mature: 495

Protein sequence:

>496_residues
MADSNILKLAPDATTPAYRTPPHNLEAERDLLGAILVNNDAFYRVSDFLEPKHFFYPVHRTIFETAGSLIRSGKIATPVT
VKTFIPADTDIGGMTASQYLARLAAEATTIINALHYGRTIYEQALRRDLIHIGEDMVNVAYDAPVDFAPKAQIEDAERRL
YELAESGGYEGGFQKFSQAMKTALDMAANAYRRDGKLSGIATGLRDLDSRMGGLQPSDLIIVAGRPGMGKTALATNIAYN
IAKAYQAELQPDGTMKTVNGGIVGFFSCEMSAEQLATRILAEQTSIASSNIRRGGITESDFEKIRDHTIELEHLPLFVDE
TGGLSISQLTARARRLKRQKGLDVLIVDYIQLLLGSGKRSDNRVQEVTEITTSLKALAKELNVPILALSQLSRQVESRDD
KRPQLSDLRESGSIEQDADVVMFVFREEYYLQNKEPRAGTPEHEKWQLDMSLVHGKAEVIIGKQRHGPTGTVELQFEGQF
TRFSDLAHEGHLPERI

Sequences:

>Translated_496_residues
MADSNILKLAPDATTPAYRTPPHNLEAERDLLGAILVNNDAFYRVSDFLEPKHFFYPVHRTIFETAGSLIRSGKIATPVT
VKTFIPADTDIGGMTASQYLARLAAEATTIINALHYGRTIYEQALRRDLIHIGEDMVNVAYDAPVDFAPKAQIEDAERRL
YELAESGGYEGGFQKFSQAMKTALDMAANAYRRDGKLSGIATGLRDLDSRMGGLQPSDLIIVAGRPGMGKTALATNIAYN
IAKAYQAELQPDGTMKTVNGGIVGFFSCEMSAEQLATRILAEQTSIASSNIRRGGITESDFEKIRDHTIELEHLPLFVDE
TGGLSISQLTARARRLKRQKGLDVLIVDYIQLLLGSGKRSDNRVQEVTEITTSLKALAKELNVPILALSQLSRQVESRDD
KRPQLSDLRESGSIEQDADVVMFVFREEYYLQNKEPRAGTPEHEKWQLDMSLVHGKAEVIIGKQRHGPTGTVELQFEGQF
TRFSDLAHEGHLPERI
>Mature_495_residues
ADSNILKLAPDATTPAYRTPPHNLEAERDLLGAILVNNDAFYRVSDFLEPKHFFYPVHRTIFETAGSLIRSGKIATPVTV
KTFIPADTDIGGMTASQYLARLAAEATTIINALHYGRTIYEQALRRDLIHIGEDMVNVAYDAPVDFAPKAQIEDAERRLY
ELAESGGYEGGFQKFSQAMKTALDMAANAYRRDGKLSGIATGLRDLDSRMGGLQPSDLIIVAGRPGMGKTALATNIAYNI
AKAYQAELQPDGTMKTVNGGIVGFFSCEMSAEQLATRILAEQTSIASSNIRRGGITESDFEKIRDHTIELEHLPLFVDET
GGLSISQLTARARRLKRQKGLDVLIVDYIQLLLGSGKRSDNRVQEVTEITTSLKALAKELNVPILALSQLSRQVESRDDK
RPQLSDLRESGSIEQDADVVMFVFREEYYLQNKEPRAGTPEHEKWQLDMSLVHGKAEVIIGKQRHGPTGTVELQFEGQFT
RFSDLAHEGHLPERI

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=481, Percent_Identity=35.3430353430353, Blast_Score=284, Evalue=1e-77,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 54908; Mature: 54777

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADSNILKLAPDATTPAYRTPPHNLEAERDLLGAILVNNDAFYRVSDFLEPKHFFYPVHR
CCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHEEEEECCCCCEEHHHHCCCHHHHHHHHH
TIFETAGSLIRSGKIATPVTVKTFIPADTDIGGMTASQYLARLAAEATTIINALHYGRTI
HHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YEQALRRDLIHIGEDMVNVAYDAPVDFAPKAQIEDAERRLYELAESGGYEGGFQKFSQAM
HHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHH
KTALDMAANAYRRDGKLSGIATGLRDLDSRMGGLQPSDLIIVAGRPGMGKTALATNIAYN
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHH
IAKAYQAELQPDGTMKTVNGGIVGFFSCEMSAEQLATRILAEQTSIASSNIRRGGITESD
HHHHHHHHCCCCCCEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
FEKIRDHTIELEHLPLFVDETGGLSISQLTARARRLKRQKGLDVLIVDYIQLLLGSGKRS
HHHHHHHCEEEEECEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCCC
DNRVQEVTEITTSLKALAKELNVPILALSQLSRQVESRDDKRPQLSDLRESGSIEQDADV
CHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCE
VMFVFREEYYLQNKEPRAGTPEHEKWQLDMSLVHGKAEVIIGKQRHGPTGTVELQFEGQF
EEEEEHHHHHHCCCCCCCCCCCCCCEEEEHHHHCCCEEEEEECCCCCCCCEEEEEECCCC
TRFSDLAHEGHLPERI
HHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ADSNILKLAPDATTPAYRTPPHNLEAERDLLGAILVNNDAFYRVSDFLEPKHFFYPVHR
CCCCEEEECCCCCCCCCCCCCCCCCHHHHHHEEEEECCCCCEEHHHHCCCHHHHHHHHH
TIFETAGSLIRSGKIATPVTVKTFIPADTDIGGMTASQYLARLAAEATTIINALHYGRTI
HHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
YEQALRRDLIHIGEDMVNVAYDAPVDFAPKAQIEDAERRLYELAESGGYEGGFQKFSQAM
HHHHHHHHHHHHCHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHH
KTALDMAANAYRRDGKLSGIATGLRDLDSRMGGLQPSDLIIVAGRPGMGKTALATNIAYN
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCHHHHHHHHHH
IAKAYQAELQPDGTMKTVNGGIVGFFSCEMSAEQLATRILAEQTSIASSNIRRGGITESD
HHHHHHHHCCCCCCEEEECCCEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHH
FEKIRDHTIELEHLPLFVDETGGLSISQLTARARRLKRQKGLDVLIVDYIQLLLGSGKRS
HHHHHHHCEEEEECEEEEECCCCCCHHHHHHHHHHHHHHCCCCEEHHHHHHHHHCCCCCC
DNRVQEVTEITTSLKALAKELNVPILALSQLSRQVESRDDKRPQLSDLRESGSIEQDADV
CHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCE
VMFVFREEYYLQNKEPRAGTPEHEKWQLDMSLVHGKAEVIIGKQRHGPTGTVELQFEGQF
EEEEEHHHHHHCCCCCCCCCCCCCCEEEEHHHHCCCEEEEEECCCCCCCCEEEEEECCCC
TRFSDLAHEGHLPERI
HHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA