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The map label for this gene is cfa [H]

Identifier: 75676202

GI number: 75676202

Start: 2200927

End: 2202171

Strand: Direct

Name: cfa [H]

Synonym: Nwi_2012

Alternate gene names: 75676202

Gene position: 2200927-2202171 (Clockwise)

Preceding gene: 75676200

Following gene: 75676203

Centisome position: 64.69

GC content: 59.68

Gene sequence:

>1245_bases
ATGGACCGTGTATTACGGTTTTTTCTGAACCAGTTTGTCCGGCGCGGCTCGATCACATTCGTAACCGCGAGCGGCCTGAC
CTTTACCTGCGGCGACGGCACCGGCGAACCTGTTCGGGTGCGGCTGACGACGAGCGAAGTCGCGCGCCAGCTTCTGCTCG
ATCCTGAAATGTCCTTCGGCGAGACCTATACCGACGGCACGTTGCTGATCGAACGGGGATCAATCGCGGACCTCCTGACG
GTAATCCTCGACCAGCCCGACGTCATGCCCCGCTGGGGGAAGTTGCAGTGGTGGCTCCGCTATCTCGTCCGCAACATCCA
GCAGTTCAACCCACGCGGGCGGTCAAGGGATAACGTCGCGCGCCATTATGACCTCGACGGCAGGCTTTATTCGCTGTTCC
TCGATGCGGACCAACAATACAGTTGCGCGTATTTTGAAACCCCCGACACGACGCTGGACGACGCCCAGCTTGCAAAGAAA
CGGCATATCGCCGCCAAGCTTCTGCTCGAACGCGATCACAGCGTCCTTGATATAGGTTCGGGCTGGGGCGGCCTCGGCCT
GTATCTCGCTGAAATGACCGGGGCCAACCTCACCGGCATTACGCTATCAACTGAACAGTTTCAGGTGTCCAACGCCCGCG
CGGTGGAGAAAAAGCTGACGCGGTCGGCGAAGTTTCTGCTCCAGGATTATCGCGACATTCCCGGACCGTTCGATCGCATC
GTATCCGTCGGAATGTTCGAGCACGTCGGCGTCGACCACTACGATACCTTTTTCAAGCGCTGCGCCGGGCTGCTCGAACC
TGACGGCGTCATGCTGCTGCATTCGATCGGCCGTTCGGAAGGTCCGGGCATCACCAATCCCTGGATCGCGAAATACATCT
TCCCCGGCGGCTACATCCCCGCGCTGTCGGAAGTCCTGCCGGCCATCGAAGGCGCCGGACTGCTGGTCTGCGACATCGAA
GTCCTGCGTCTGCACTATGCGGAAACGCTGAAGGCATGGCGGGAGCGCTTCATGGCGCGACGCGAGGAAGCGGTGCGGCT
ATATGACGAGCGGTTCGCGCGGATGTGGGAATTTTATCTGGCGGCATCGGAAATGTCGTTCCGGAAGCAGAATCTGATGA
ATTTCCAGATTCAGATCACGAAACGGCAAGGCATCGTGCCTGTAACGCGCGACTACATCCAGCGCGGGGAAGCAAAGCTG
CGCGGTCTGGAGCAAAAACACCGGCCCCGGCTTCAAATCGCGTGA

Upstream 100 bases:

>100_bases
CGTCGCTTAACTTTTGTTTGAGCATGATCTCATCCGAAAGCCGGTTTCCACTTTTCGGGATCATGCTCTAGCGCGCGTCC
TCGTGTTGATGGTGGTTTCG

Downstream 100 bases:

>100_bases
GAATGATGCATGATCAACAGCAGCTTATGCTTCCTGCATCGCGGCTCGACAATGAGTCGCGGAGAAGGTTCAGCCGGCAG
TCAAGTTCGTTGCGTTATCG

Product: cyclopropane-fatty-acyl-phospholipid synthase

Products: NA

Alternate protein names: CFA synthase; Cyclopropane fatty acid synthase [H]

Number of amino acids: Translated: 414; Mature: 414

Protein sequence:

>414_residues
MDRVLRFFLNQFVRRGSITFVTASGLTFTCGDGTGEPVRVRLTTSEVARQLLLDPEMSFGETYTDGTLLIERGSIADLLT
VILDQPDVMPRWGKLQWWLRYLVRNIQQFNPRGRSRDNVARHYDLDGRLYSLFLDADQQYSCAYFETPDTTLDDAQLAKK
RHIAAKLLLERDHSVLDIGSGWGGLGLYLAEMTGANLTGITLSTEQFQVSNARAVEKKLTRSAKFLLQDYRDIPGPFDRI
VSVGMFEHVGVDHYDTFFKRCAGLLEPDGVMLLHSIGRSEGPGITNPWIAKYIFPGGYIPALSEVLPAIEGAGLLVCDIE
VLRLHYAETLKAWRERFMARREEAVRLYDERFARMWEFYLAASEMSFRKQNLMNFQIQITKRQGIVPVTRDYIQRGEAKL
RGLEQKHRPRLQIA

Sequences:

>Translated_414_residues
MDRVLRFFLNQFVRRGSITFVTASGLTFTCGDGTGEPVRVRLTTSEVARQLLLDPEMSFGETYTDGTLLIERGSIADLLT
VILDQPDVMPRWGKLQWWLRYLVRNIQQFNPRGRSRDNVARHYDLDGRLYSLFLDADQQYSCAYFETPDTTLDDAQLAKK
RHIAAKLLLERDHSVLDIGSGWGGLGLYLAEMTGANLTGITLSTEQFQVSNARAVEKKLTRSAKFLLQDYRDIPGPFDRI
VSVGMFEHVGVDHYDTFFKRCAGLLEPDGVMLLHSIGRSEGPGITNPWIAKYIFPGGYIPALSEVLPAIEGAGLLVCDIE
VLRLHYAETLKAWRERFMARREEAVRLYDERFARMWEFYLAASEMSFRKQNLMNFQIQITKRQGIVPVTRDYIQRGEAKL
RGLEQKHRPRLQIA
>Mature_414_residues
MDRVLRFFLNQFVRRGSITFVTASGLTFTCGDGTGEPVRVRLTTSEVARQLLLDPEMSFGETYTDGTLLIERGSIADLLT
VILDQPDVMPRWGKLQWWLRYLVRNIQQFNPRGRSRDNVARHYDLDGRLYSLFLDADQQYSCAYFETPDTTLDDAQLAKK
RHIAAKLLLERDHSVLDIGSGWGGLGLYLAEMTGANLTGITLSTEQFQVSNARAVEKKLTRSAKFLLQDYRDIPGPFDRI
VSVGMFEHVGVDHYDTFFKRCAGLLEPDGVMLLHSIGRSEGPGITNPWIAKYIFPGGYIPALSEVLPAIEGAGLLVCDIE
VLRLHYAETLKAWRERFMARREEAVRLYDERFARMWEFYLAASEMSFRKQNLMNFQIQITKRQGIVPVTRDYIQRGEAKL
RGLEQKHRPRLQIA

Specific function: Transfers a methylene group from S-adenosyl-L-methionine to the cis double bond of an unsaturated fatty acid chain resulting in the replacement of the double bond with a methylene bridge [H]

COG id: COG2230

COG function: function code M; Cyclopropane fatty acid synthase and related methyltransferases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CFA/CMAS family [H]

Homologues:

Organism=Escherichia coli, GI1787951, Length=347, Percent_Identity=36.3112391930836, Blast_Score=206, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI133904226, Length=371, Percent_Identity=28.5714285714286, Blast_Score=127, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003333 [H]

Pfam domain/function: PF02353 CMAS [H]

EC number: =2.1.1.79 [H]

Molecular weight: Translated: 47355; Mature: 47355

Theoretical pI: Translated: 8.29; Mature: 8.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRVLRFFLNQFVRRGSITFVTASGLTFTCGDGTGEPVRVRLTTSEVARQLLLDPEMSFG
CHHHHHHHHHHHHHCCCEEEEEECCEEEEECCCCCCCEEEEEEHHHHHHHHHCCCCCCCC
ETYTDGTLLIERGSIADLLTVILDQPDVMPRWGKLQWWLRYLVRNIQQFNPRGRSRDNVA
CEECCCEEEEECCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHH
RHYDLDGRLYSLFLDADQQYSCAYFETPDTTLDDAQLAKKRHIAAKLLLERDHSVLDIGS
HHCCCCCEEEEEEECCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECC
GWGGLGLYLAEMTGANLTGITLSTEQFQVSNARAVEKKLTRSAKFLLQDYRDIPGPFDRI
CCCHHHHHHHHHCCCCEEEEEEECHHEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
VSVGMFEHVGVDHYDTFFKRCAGLLEPDGVMLLHSIGRSEGPGITNPWIAKYIFPGGYIP
HHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHEECCCCCCCCCCCCCHHHHHCCCCCCHH
ALSEVLPAIEGAGLLVCDIEVLRLHYAETLKAWRERFMARREEAVRLYDERFARMWEFYL
HHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AASEMSFRKQNLMNFQIQITKRQGIVPVTRDYIQRGEAKLRGLEQKHRPRLQIA
HHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHCCHHHHHCCHHHCCCCCCCC
>Mature Secondary Structure
MDRVLRFFLNQFVRRGSITFVTASGLTFTCGDGTGEPVRVRLTTSEVARQLLLDPEMSFG
CHHHHHHHHHHHHHCCCEEEEEECCEEEEECCCCCCCEEEEEEHHHHHHHHHCCCCCCCC
ETYTDGTLLIERGSIADLLTVILDQPDVMPRWGKLQWWLRYLVRNIQQFNPRGRSRDNVA
CEECCCEEEEECCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCHH
RHYDLDGRLYSLFLDADQQYSCAYFETPDTTLDDAQLAKKRHIAAKLLLERDHSVLDIGS
HHCCCCCEEEEEEECCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECC
GWGGLGLYLAEMTGANLTGITLSTEQFQVSNARAVEKKLTRSAKFLLQDYRDIPGPFDRI
CCCHHHHHHHHHCCCCEEEEEEECHHEECCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHH
VSVGMFEHVGVDHYDTFFKRCAGLLEPDGVMLLHSIGRSEGPGITNPWIAKYIFPGGYIP
HHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHEECCCCCCCCCCCCCHHHHHCCCCCCHH
ALSEVLPAIEGAGLLVCDIEVLRLHYAETLKAWRERFMARREEAVRLYDERFARMWEFYL
HHHHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AASEMSFRKQNLMNFQIQITKRQGIVPVTRDYIQRGEAKLRGLEQKHRPRLQIA
HHHHHHHHHHCCCEEEEEEECCCCCCCHHHHHHHCCHHHHHCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]