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The map label for this gene is hemB [H]

Identifier: 75676100

GI number: 75676100

Start: 2094384

End: 2095442

Strand: Direct

Name: hemB [H]

Synonym: Nwi_1909

Alternate gene names: 75676100

Gene position: 2094384-2095442 (Clockwise)

Preceding gene: 75676096

Following gene: 75676101

Centisome position: 61.56

GC content: 64.78

Gene sequence:

>1059_bases
ATGGCGATAAAATACGGGCGTCCCATTGAAATGCGGGATGGACCCCGCCGCGATGCCGCAACAGCCGCTCCGCTCGATCT
CGTCGCCCGCCTCCGCCGTAACCGCAAGGCGGAATGGGCGCGCCGCCTGGTCCGCGAAAGCGTTCTGACCACCGACGATC
TCATCTGGCCGCTGTTCGTCGCCGATGGCGCCGACAGGCGGACGCCCGTCCCCTCCATGCCCGGTGTCGACCGTCTCAGC
GTGGACGAATGCGTGCGGGACGCGGAACGGGCGCTCGAGCTGAACATCCCTTGCATCGCGCTTTTCCCCTATACCGATCC
CGCCTTGCGGGACCTCTCCGGCAGCGAAGCCCTCAACGCCAAGAATCTGGTCTGCAAGTCGGTCAGGGCCATCAAGCGGG
AGTTTCCGGACCTCGGCGTACTTTGCGATGTGGCGCTCGATCCCTTCACCAGTCATGGCCATGACGGTCTGGTCGAGGAC
GGCAGGATCCTCAACGACGAGACCGTCGCGGTGCTGGTCCAGCAGGCGCTGGTGCAGGCGGAAGCCGGTTGCGACATCGT
CGCGCCGTCCGACATGATGGACGGCCGCGTCGGTGCAATTCGAAGAGCCCTGGACGAGGCCGGGTTCGTCGATGTGCAGA
TCATGGCCTATGCCGCGAAGTATGCATCCGCGTTTTACGGCCCGTTCCGCGACGCCATCGGCTCGGCCAAGGCGCTCACG
GGAGATAAACGCACATACCAGATGGACAGCGCCAACTCCGATGAAGCTCTGCGCGAAGTCGAACTCGATATCGCGGAGGG
CGCCGACATGGTGCTGGTGAAGCCGGGAATGCCCTATCTCGACATCGTGCGGCGCGTAAAGGACACGTTCGGAATGCCGA
CCTTCGCCTACCAGGTGTCGGGCGAGTACGCGATGATCGCGGCCGCCGCCGGCAACGGCTGGATCGATCGCGACCGGGCG
ATGATGGAAAGCCTCGTCGCCTTCAAGCGCGCCGGCGCCGATGGCGTGCTCACCTATTTTGCGGCGGAGGCGGCGGAGAA
GCTGAAACAGAACCGCTGA

Upstream 100 bases:

>100_bases
ATCAATTGCATTGCGTCGAGGGGCATGAGCGCGGTGACGAAGCCGGTTCCTGGTGCTACATAAAACCGGTTCGGTTCTGG
CCTTCAGGAGTGTCGGCAAC

Downstream 100 bases:

>100_bases
CGTCCCCTCCTCCCCGGCGATCTCCGTCCGCTCCTCTGAGCCTGCCTGGGGGTTGCAACCCCGACAGCACGTCGCCATCT
CGAAGGGCGCTTCATACTTT

Product: delta-aminolevulinic acid dehydratase

Products: NA

Alternate protein names: ALAD; ALADH; Porphobilinogen synthase [H]

Number of amino acids: Translated: 352; Mature: 351

Protein sequence:

>352_residues
MAIKYGRPIEMRDGPRRDAATAAPLDLVARLRRNRKAEWARRLVRESVLTTDDLIWPLFVADGADRRTPVPSMPGVDRLS
VDECVRDAERALELNIPCIALFPYTDPALRDLSGSEALNAKNLVCKSVRAIKREFPDLGVLCDVALDPFTSHGHDGLVED
GRILNDETVAVLVQQALVQAEAGCDIVAPSDMMDGRVGAIRRALDEAGFVDVQIMAYAAKYASAFYGPFRDAIGSAKALT
GDKRTYQMDSANSDEALREVELDIAEGADMVLVKPGMPYLDIVRRVKDTFGMPTFAYQVSGEYAMIAAAAGNGWIDRDRA
MMESLVAFKRAGADGVLTYFAAEAAEKLKQNR

Sequences:

>Translated_352_residues
MAIKYGRPIEMRDGPRRDAATAAPLDLVARLRRNRKAEWARRLVRESVLTTDDLIWPLFVADGADRRTPVPSMPGVDRLS
VDECVRDAERALELNIPCIALFPYTDPALRDLSGSEALNAKNLVCKSVRAIKREFPDLGVLCDVALDPFTSHGHDGLVED
GRILNDETVAVLVQQALVQAEAGCDIVAPSDMMDGRVGAIRRALDEAGFVDVQIMAYAAKYASAFYGPFRDAIGSAKALT
GDKRTYQMDSANSDEALREVELDIAEGADMVLVKPGMPYLDIVRRVKDTFGMPTFAYQVSGEYAMIAAAAGNGWIDRDRA
MMESLVAFKRAGADGVLTYFAAEAAEKLKQNR
>Mature_351_residues
AIKYGRPIEMRDGPRRDAATAAPLDLVARLRRNRKAEWARRLVRESVLTTDDLIWPLFVADGADRRTPVPSMPGVDRLSV
DECVRDAERALELNIPCIALFPYTDPALRDLSGSEALNAKNLVCKSVRAIKREFPDLGVLCDVALDPFTSHGHDGLVEDG
RILNDETVAVLVQQALVQAEAGCDIVAPSDMMDGRVGAIRRALDEAGFVDVQIMAYAAKYASAFYGPFRDAIGSAKALTG
DKRTYQMDSANSDEALREVELDIAEGADMVLVKPGMPYLDIVRRVKDTFGMPTFAYQVSGEYAMIAAAAGNGWIDRDRAM
MESLVAFKRAGADGVLTYFAAEAAEKLKQNR

Specific function: Catalyzes an early step in the biosynthesis of tetrapyrroles. Binds two molecules of 5-aminolevulinate per subunit, each at a distinct site, and catalyzes their condensation to form porphobilinogen. Required for nodule development [H]

COG id: COG0113

COG function: function code H; Delta-aminolevulinic acid dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ALADH family [H]

Homologues:

Organism=Homo sapiens, GI189083849, Length=308, Percent_Identity=43.8311688311688, Blast_Score=247, Evalue=1e-65,
Organism=Escherichia coli, GI87081728, Length=322, Percent_Identity=45.3416149068323, Blast_Score=254, Evalue=6e-69,
Organism=Saccharomyces cerevisiae, GI6321398, Length=332, Percent_Identity=37.3493975903614, Blast_Score=199, Evalue=8e-52,
Organism=Drosophila melanogaster, GI21358291, Length=293, Percent_Identity=41.9795221843003, Blast_Score=245, Evalue=4e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001731
- InterPro:   IPR013785 [H]

Pfam domain/function: PF00490 ALAD [H]

EC number: =4.2.1.24 [H]

Molecular weight: Translated: 38521; Mature: 38390

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: PS00169 D_ALA_DEHYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIKYGRPIEMRDGPRRDAATAAPLDLVARLRRNRKAEWARRLVRESVLTTDDLIWPLFV
CCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHEE
ADGADRRTPVPSMPGVDRLSVDECVRDAERALELNIPCIALFPYTDPALRDLSGSEALNA
ECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHEECCCCEEEEECCCCHHHHCCCCCCCCCH
KNLVCKSVRAIKREFPDLGVLCDVALDPFTSHGHDGLVEDGRILNDETVAVLVQQALVQA
HHHHHHHHHHHHHHCCCCCEEEHHHCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHH
EAGCDIVAPSDMMDGRVGAIRRALDEAGFVDVQIMAYAAKYASAFYGPFRDAIGSAKALT
HCCCCEECCCHHHCCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCHHCC
GDKRTYQMDSANSDEALREVELDIAEGADMVLVKPGMPYLDIVRRVKDTFGMPTFAYQVS
CCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEEC
GEYAMIAAAAGNGWIDRDRAMMESLVAFKRAGADGVLTYFAAEAAEKLKQNR
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AIKYGRPIEMRDGPRRDAATAAPLDLVARLRRNRKAEWARRLVRESVLTTDDLIWPLFV
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCHHHHHHHHEE
ADGADRRTPVPSMPGVDRLSVDECVRDAERALELNIPCIALFPYTDPALRDLSGSEALNA
ECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHEECCCCEEEEECCCCHHHHCCCCCCCCCH
KNLVCKSVRAIKREFPDLGVLCDVALDPFTSHGHDGLVEDGRILNDETVAVLVQQALVQA
HHHHHHHHHHHHHHCCCCCEEEHHHCCCCCCCCCCCCCCCCEEECHHHHHHHHHHHHHHH
EAGCDIVAPSDMMDGRVGAIRRALDEAGFVDVQIMAYAAKYASAFYGPFRDAIGSAKALT
HCCCCEECCCHHHCCHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCHHCC
GDKRTYQMDSANSDEALREVELDIAEGADMVLVKPGMPYLDIVRRVKDTFGMPTFAYQVS
CCCCEEEECCCCCHHHHHHHHHHHHCCCCEEEECCCCCHHHHHHHHHHHHCCCEEEEEEC
GEYAMIAAAAGNGWIDRDRAMMESLVAFKRAGADGVLTYFAAEAAEKLKQNR
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8226669; 12597275 [H]