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The map label for this gene is glnA [H]

Identifier: 75676095

GI number: 75676095

Start: 2089515

End: 2090924

Strand: Direct

Name: glnA [H]

Synonym: Nwi_1904

Alternate gene names: 75676095

Gene position: 2089515-2090924 (Clockwise)

Preceding gene: 75676094

Following gene: 75676096

Centisome position: 61.42

GC content: 59.93

Gene sequence:

>1410_bases
ATGAAGACCGCCAATGACGTCCTGAAATCGATCAAGGACAACGACGTAAAATACGTCGATCTGCGTTTCACCGATCCGCG
CGGCAAGTGGCAGCATGTCACCTTCGACATCAGCATGATCGACGAAGAGTTTTTCGCCGAGGGTACGATGTTCGACGGCT
CATCGATTGCCGGCTGGAAGGTGATCAACGAGTCCGACATGACGCTGATGCCCGATCCGGCGACGGCGACAATCGATCCG
TTCTTCGCGGAAACCACCATGGTGCTCACCTGCGACATCCTGGAGCCGTCCACGGGCGAGCCCTATAACCGTGACCCTCG
CGGGATCGCCAAGAAGGCCGAGGCGATGGTGAAGTCGATGGGCATCGGCGATACGGTCTATGTCGGCCCGGAAGCCGAAT
TCTTCGTGTTCGACGACGTGCGCTTTCAGACCACGCCTTACAACACCGGCTTCAAGCTCGATTCCTCCGAGCTGCCGACC
AACTCCGATACCGAGTATGAGGGCGGCAATCTCGGTCATCGCATCCGCACCAAGGGCGGCTACTTCCCGGTTCCACCACA
GGACTCGATCCAGGACATGCGTTCCGAGATGCTCGGCGCCATGGCCCGCATGGGCGTGAAGGTCGAGAAGCATCATCACG
AGGTGGCGTCCGCCCAGCACGAACTCGGCATGAAGTTCGATACGCTGACCTACATGGCCGACCAGATGCAGGTATACAAA
TACTGCATTCACCAGGTCGCGCACATCTACGGCAAGACCGCCACCTTCATGCCGAAGCCGGTCTACGGCGACAACGGCTC
GGGCATGCACGTTCACCAATCGATCTGGAAGGACGGCAAGCCGACCTTCGCCGGCAGCAAATACGCCGACCTGTCGGAAA
CCTGCCTGTACTACATCGGCGGCATCATCAAGCACGCCAAGGCCATCAACGCCTTCACCAACCCGTCCACCAACTCCTAC
AAGCGGCTGGTGCCGGGATACGAGGCGCCGGTGCTGCTCGCCTATTCGGCGCGCAACCGCTCCGCCTCCTGCCGCATCCC
CTACACCACCTCGCCGAAGGCCAAGCGCGTCGAGGTTCGCTTCCCCGACCCGATGGCCAATCCGTATCTCGCCTTCGCGG
CGATGCTGATGGCGGGCCTGGACGGGATCAAGAACAAGATCGATCCCGGCCCCGCCATGGACAAGGACCTCTATGACCTG
CCGAAGGAAGAACTGAAGCAGATTCCCACGGTCTGCGGTTCATTGCGCGAGGCGCTGGAAAGCCTCGATAAGGATCGCGC
CTTCCTCAAGGCCGGCGGCGTGTTCGACGACGACTTCATCAACGCCTTCATCGAATTGAAGATGATCGAGGTGGAGCGCT
TCGAGATGACGCCGCACCCGGTCGAATTCGATATGTATTACTCGCTGTAG

Upstream 100 bases:

>100_bases
CGCCATCTAAATCCTTTGAGAACGGACATCACTCATTAAGCCATCCCGACGGCCGCTTCGCGCCGGCGATTACGACGACC
AGCAGAGACAAGGGGTGTTT

Downstream 100 bases:

>100_bases
GCTGCGACCCGCGCTGCCTCTCCACAGCTTTCGAGAATCTGAAGGGCGCCCATTACAAGGCGCCCTTTTTGTTGATGGTC
GTGGTCCGCAAGGGTCGCGG

Product: glutamine synthetase type I

Products: NA

Alternate protein names: Glutamate--ammonia ligase I; Glutamine synthetase I; GSI [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MKTANDVLKSIKDNDVKYVDLRFTDPRGKWQHVTFDISMIDEEFFAEGTMFDGSSIAGWKVINESDMTLMPDPATATIDP
FFAETTMVLTCDILEPSTGEPYNRDPRGIAKKAEAMVKSMGIGDTVYVGPEAEFFVFDDVRFQTTPYNTGFKLDSSELPT
NSDTEYEGGNLGHRIRTKGGYFPVPPQDSIQDMRSEMLGAMARMGVKVEKHHHEVASAQHELGMKFDTLTYMADQMQVYK
YCIHQVAHIYGKTATFMPKPVYGDNGSGMHVHQSIWKDGKPTFAGSKYADLSETCLYYIGGIIKHAKAINAFTNPSTNSY
KRLVPGYEAPVLLAYSARNRSASCRIPYTTSPKAKRVEVRFPDPMANPYLAFAAMLMAGLDGIKNKIDPGPAMDKDLYDL
PKEELKQIPTVCGSLREALESLDKDRAFLKAGGVFDDDFINAFIELKMIEVERFEMTPHPVEFDMYYSL

Sequences:

>Translated_469_residues
MKTANDVLKSIKDNDVKYVDLRFTDPRGKWQHVTFDISMIDEEFFAEGTMFDGSSIAGWKVINESDMTLMPDPATATIDP
FFAETTMVLTCDILEPSTGEPYNRDPRGIAKKAEAMVKSMGIGDTVYVGPEAEFFVFDDVRFQTTPYNTGFKLDSSELPT
NSDTEYEGGNLGHRIRTKGGYFPVPPQDSIQDMRSEMLGAMARMGVKVEKHHHEVASAQHELGMKFDTLTYMADQMQVYK
YCIHQVAHIYGKTATFMPKPVYGDNGSGMHVHQSIWKDGKPTFAGSKYADLSETCLYYIGGIIKHAKAINAFTNPSTNSY
KRLVPGYEAPVLLAYSARNRSASCRIPYTTSPKAKRVEVRFPDPMANPYLAFAAMLMAGLDGIKNKIDPGPAMDKDLYDL
PKEELKQIPTVCGSLREALESLDKDRAFLKAGGVFDDDFINAFIELKMIEVERFEMTPHPVEFDMYYSL
>Mature_469_residues
MKTANDVLKSIKDNDVKYVDLRFTDPRGKWQHVTFDISMIDEEFFAEGTMFDGSSIAGWKVINESDMTLMPDPATATIDP
FFAETTMVLTCDILEPSTGEPYNRDPRGIAKKAEAMVKSMGIGDTVYVGPEAEFFVFDDVRFQTTPYNTGFKLDSSELPT
NSDTEYEGGNLGHRIRTKGGYFPVPPQDSIQDMRSEMLGAMARMGVKVEKHHHEVASAQHELGMKFDTLTYMADQMQVYK
YCIHQVAHIYGKTATFMPKPVYGDNGSGMHVHQSIWKDGKPTFAGSKYADLSETCLYYIGGIIKHAKAINAFTNPSTNSY
KRLVPGYEAPVLLAYSARNRSASCRIPYTTSPKAKRVEVRFPDPMANPYLAFAAMLMAGLDGIKNKIDPGPAMDKDLYDL
PKEELKQIPTVCGSLREALESLDKDRAFLKAGGVFDDDFINAFIELKMIEVERFEMTPHPVEFDMYYSL

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=416, Percent_Identity=24.5192307692308, Blast_Score=87, Evalue=3e-17,
Organism=Escherichia coli, GI1790301, Length=468, Percent_Identity=65.1709401709402, Blast_Score=641, Evalue=0.0,
Organism=Escherichia coli, GI87081870, Length=396, Percent_Identity=24.7474747474747, Blast_Score=86, Evalue=6e-18,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809
- InterPro:   IPR001637 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 52590; Mature: 52590

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP ; PS00182 GLNA_ADENYLATION

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
5.1 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTANDVLKSIKDNDVKYVDLRFTDPRGKWQHVTFDISMIDEEFFAEGTMFDGSSIAGWK
CCCHHHHHHHHCCCCEEEEEEEEECCCCCEEEEEEEEEEHHHHHHCCCCEECCCCCCCEE
VINESDMTLMPDPATATIDPFFAETTMVLTCDILEPSTGEPYNRDPRGIAKKAEAMVKSM
EECCCCCEECCCCCCCCCCCHHHCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHC
GIGDTVYVGPEAEFFVFDDVRFQTTPYNTGFKLDSSELPTNSDTEYEGGNLGHRIRTKGG
CCCCEEEECCCCCEEEEECEEEEECCCCCCCEECCCCCCCCCCCCCCCCCCCCEEECCCC
YFPVPPQDSIQDMRSEMLGAMARMGVKVEKHHHEVASAQHELGMKFDTLTYMADQMQVYK
CCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
YCIHQVAHIYGKTATFMPKPVYGDNGSGMHVHQSIWKDGKPTFAGSKYADLSETCLYYIG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
GIIKHAKAINAFTNPSTNSYKRLVPGYEAPVLLAYSARNRSASCRIPYTTSPKAKRVEVR
HHHHHHHHHHCCCCCCCCCHHHCCCCCCCCEEEEEECCCCCCEEECCCCCCCCCCEEEEE
FPDPMANPYLAFAAMLMAGLDGIKNKIDPGPAMDKDLYDLPKEELKQIPTVCGSLREALE
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHH
SLDKDRAFLKAGGVFDDDFINAFIELKMIEVERFEMTPHPVEFDMYYSL
HHHHHHHHHHCCCCCCHHHHHHHHEEEEEEEEEEECCCCCEEEEEEECC
>Mature Secondary Structure
MKTANDVLKSIKDNDVKYVDLRFTDPRGKWQHVTFDISMIDEEFFAEGTMFDGSSIAGWK
CCCHHHHHHHHCCCCEEEEEEEEECCCCCEEEEEEEEEEHHHHHHCCCCEECCCCCCCEE
VINESDMTLMPDPATATIDPFFAETTMVLTCDILEPSTGEPYNRDPRGIAKKAEAMVKSM
EECCCCCEECCCCCCCCCCCHHHCEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHHHC
GIGDTVYVGPEAEFFVFDDVRFQTTPYNTGFKLDSSELPTNSDTEYEGGNLGHRIRTKGG
CCCCEEEECCCCCEEEEECEEEEECCCCCCCEECCCCCCCCCCCCCCCCCCCCEEECCCC
YFPVPPQDSIQDMRSEMLGAMARMGVKVEKHHHEVASAQHELGMKFDTLTYMADQMQVYK
CCCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
YCIHQVAHIYGKTATFMPKPVYGDNGSGMHVHQSIWKDGKPTFAGSKYADLSETCLYYIG
HHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
GIIKHAKAINAFTNPSTNSYKRLVPGYEAPVLLAYSARNRSASCRIPYTTSPKAKRVEVR
HHHHHHHHHHCCCCCCCCCHHHCCCCCCCCEEEEEECCCCCCEEECCCCCCCCCCEEEEE
FPDPMANPYLAFAAMLMAGLDGIKNKIDPGPAMDKDLYDLPKEELKQIPTVCGSLREALE
CCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHH
SLDKDRAFLKAGGVFDDDFINAFIELKMIEVERFEMTPHPVEFDMYYSL
HHHHHHHHHHCCCCCCHHHHHHHHEEEEEEEEEEECCCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275; 2859270; 2793830 [H]