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The map label for this gene is gltA [H]

Identifier: 75676036

GI number: 75676036

Start: 2022697

End: 2023998

Strand: Direct

Name: gltA [H]

Synonym: Nwi_1845

Alternate gene names: 75676036

Gene position: 2022697-2023998 (Clockwise)

Preceding gene: 75676035

Following gene: 75676080

Centisome position: 59.45

GC content: 58.14

Gene sequence:

>1302_bases
ATGAACGCAACGAGCACAAAAAACGCAACGTTGACGGTCGATGACAAGAGTTACGAGTTTCCCGTATTGAGCGGCACGGT
CGGGCCTGATGTCGTCGACATCGGCAAGCTCTATGCGCAGACAGGCATGTTCACTTACGATCCTGCCTTCACATCCACCG
CCAGTTGCCAATCCAAGATCACCTATATCGACGGCGACGAAGGCGTGCTCGAATATCGCGGCTACCCGATCGAGCAGCTC
GCGGAGAAAGGCGACTTCATCGAGACCTGCTATCTGCTGCTCTACGGTGAACTGCCCACGAAGTCGCAGAAGCACGATTT
CGAGGAGCGTGTCACGCACCACACCATGGTTCATGAGCAGATGACGCGGTTCTTCCAGAGCTTCCGCCGCGATGCCCATC
CCATGGCGGTCATGGTGGCGGCGGTCGGCGCGCTCGCCGCCTTCTATCACGACTCAACCGACATCAACGATCCCAAGCAG
CGCATGATCGCGTCGATGCGCATGGTCGCGAAAATGCCGACGCTGGCTGCGATGGCTTACAAGTATACGATCGGCCAGCC
GTTCATATATCCGAAGAACTCCCTGAACTTTGCCGAGAACTTCCTTCATATGTGCTTCGCCGTGCCGTGCGAGGACTATG
ACATCAATCCGGTTCTGGCGGACGCGCTCGACAAGATCTTCATTCTGCACGCCGACCACGAACAGAACGCATCGACATCG
ACCGTGCGCATCGCCGGTTCCTCAGGCGCCAATCCTTTCGCGTGCATTGCGGCCGGCATCGCCTGCCTGTGGGGACCCGC
GCACGGCGGCGCCAACGAGGCGGCGCTGAACATGCTTTACGAGATCGGGACGGTAGACAGAATTCCAGAATTCATCGACA
AGGTGAAGGACAAGAACAGCGATGTCCGGCTGATGGGTTTCGGACATCGGGTCTACAAGAACTACGATCCGCGCGCGAAG
ATCATGCAGAACATGTGTCACGCGGTGCTCAACGAGACGGGCCACGGCAATGATCCGATGCTCAAGGTGGCGCTGGAACT
GGAAAAGATCGCTCTGAACGACGAGTATTTCATCAATCGCAAGCTGTATCCCAACGTCGATTTCTATTCGGGCCTCACCC
TGAAAGCGATGGGCTTTCCGACCTCGATGTTCACCGTGCTGTTCGCGGTCGCGCGCACTGTGGGCTGGATCAGCCAGTGG
AGCGAGATGATCGAGGACCCGCAGCAGAAGATCGGCCGTCCCCGGCAGCTTTACACAGGCAAGACCCGGCGCGATTACGT
CGACATGGCTCAGCGGGCGTAG

Upstream 100 bases:

>100_bases
AATACGATACCCCTTTGCGCGGCGCTTTTGAATGACCGGCTGGTTTCTCTCGTCGAGAGAGACCGGGTGATCCGCAACGA
TCTCATCGGGGGGCTTCACG

Downstream 100 bases:

>100_bases
TTTATTCTTGCGAAGCTGGTCGCCTACATTCGCGCCCGCGACTCTCGCATGGCTTCGATCACGATATCGGCTGCCAGCTC
GCTCGGCGAGCGCTGCCCTG

Product: type II citrate synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 433; Mature: 433

Protein sequence:

>433_residues
MNATSTKNATLTVDDKSYEFPVLSGTVGPDVVDIGKLYAQTGMFTYDPAFTSTASCQSKITYIDGDEGVLEYRGYPIEQL
AEKGDFIETCYLLLYGELPTKSQKHDFEERVTHHTMVHEQMTRFFQSFRRDAHPMAVMVAAVGALAAFYHDSTDINDPKQ
RMIASMRMVAKMPTLAAMAYKYTIGQPFIYPKNSLNFAENFLHMCFAVPCEDYDINPVLADALDKIFILHADHEQNASTS
TVRIAGSSGANPFACIAAGIACLWGPAHGGANEAALNMLYEIGTVDRIPEFIDKVKDKNSDVRLMGFGHRVYKNYDPRAK
IMQNMCHAVLNETGHGNDPMLKVALELEKIALNDEYFINRKLYPNVDFYSGLTLKAMGFPTSMFTVLFAVARTVGWISQW
SEMIEDPQQKIGRPRQLYTGKTRRDYVDMAQRA

Sequences:

>Translated_433_residues
MNATSTKNATLTVDDKSYEFPVLSGTVGPDVVDIGKLYAQTGMFTYDPAFTSTASCQSKITYIDGDEGVLEYRGYPIEQL
AEKGDFIETCYLLLYGELPTKSQKHDFEERVTHHTMVHEQMTRFFQSFRRDAHPMAVMVAAVGALAAFYHDSTDINDPKQ
RMIASMRMVAKMPTLAAMAYKYTIGQPFIYPKNSLNFAENFLHMCFAVPCEDYDINPVLADALDKIFILHADHEQNASTS
TVRIAGSSGANPFACIAAGIACLWGPAHGGANEAALNMLYEIGTVDRIPEFIDKVKDKNSDVRLMGFGHRVYKNYDPRAK
IMQNMCHAVLNETGHGNDPMLKVALELEKIALNDEYFINRKLYPNVDFYSGLTLKAMGFPTSMFTVLFAVARTVGWISQW
SEMIEDPQQKIGRPRQLYTGKTRRDYVDMAQRA
>Mature_433_residues
MNATSTKNATLTVDDKSYEFPVLSGTVGPDVVDIGKLYAQTGMFTYDPAFTSTASCQSKITYIDGDEGVLEYRGYPIEQL
AEKGDFIETCYLLLYGELPTKSQKHDFEERVTHHTMVHEQMTRFFQSFRRDAHPMAVMVAAVGALAAFYHDSTDINDPKQ
RMIASMRMVAKMPTLAAMAYKYTIGQPFIYPKNSLNFAENFLHMCFAVPCEDYDINPVLADALDKIFILHADHEQNASTS
TVRIAGSSGANPFACIAAGIACLWGPAHGGANEAALNMLYEIGTVDRIPEFIDKVKDKNSDVRLMGFGHRVYKNYDPRAK
IMQNMCHAVLNETGHGNDPMLKVALELEKIALNDEYFINRKLYPNVDFYSGLTLKAMGFPTSMFTVLFAVARTVGWISQW
SEMIEDPQQKIGRPRQLYTGKTRRDYVDMAQRA

Specific function: Tricarboxylic acid cycle. [C]

COG id: COG0372

COG function: function code C; Citrate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the citrate synthase family [H]

Homologues:

Organism=Homo sapiens, GI38327625, Length=394, Percent_Identity=28.6802030456853, Blast_Score=110, Evalue=3e-24,
Organism=Escherichia coli, GI1786939, Length=425, Percent_Identity=65.1764705882353, Blast_Score=596, Evalue=1e-171,
Organism=Escherichia coli, GI1786527, Length=365, Percent_Identity=30.1369863013699, Blast_Score=157, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI17555174, Length=390, Percent_Identity=28.2051282051282, Blast_Score=117, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6319850, Length=358, Percent_Identity=26.536312849162, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324328, Length=366, Percent_Identity=25.6830601092896, Blast_Score=99, Evalue=2e-21,
Organism=Saccharomyces cerevisiae, GI6325257, Length=441, Percent_Identity=25.1700680272109, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24640126, Length=397, Percent_Identity=27.2040302267003, Blast_Score=111, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24640124, Length=397, Percent_Identity=27.2040302267003, Blast_Score=111, Evalue=9e-25,
Organism=Drosophila melanogaster, GI21356863, Length=394, Percent_Identity=27.9187817258883, Blast_Score=108, Evalue=6e-24,

Paralogues:

None

Copy number: 624 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016142
- InterPro:   IPR016143
- InterPro:   IPR002020
- InterPro:   IPR016141
- InterPro:   IPR019810
- InterPro:   IPR010953 [H]

Pfam domain/function: PF00285 Citrate_synt [H]

EC number: =2.3.3.1 [H]

Molecular weight: Translated: 48582; Mature: 48582

Theoretical pI: Translated: 6.19; Mature: 6.19

Prosite motif: PS00480 CITRATE_SYNTHASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
4.8 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
4.8 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNATSTKNATLTVDDKSYEFPVLSGTVGPDVVDIGKLYAQTGMFTYDPAFTSTASCQSKI
CCCCCCCCCEEEECCCCCCCCEEECCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCEE
TYIDGDEGVLEYRGYPIEQLAEKGDFIETCYLLLYGELPTKSQKHDFEERVTHHTMVHEQ
EEEECCCCEEEECCCCHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
MTRFFQSFRRDAHPMAVMVAAVGALAAFYHDSTDINDPKQRMIASMRMVAKMPTLAAMAY
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHH
KYTIGQPFIYPKNSLNFAENFLHMCFAVPCEDYDINPVLADALDKIFILHADHEQNASTS
HHCCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHCEEEEEECCCCCCCCCC
TVRIAGSSGANPFACIAAGIACLWGPAHGGANEAALNMLYEIGTVDRIPEFIDKVKDKNS
EEEEECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
DVRLMGFGHRVYKNYDPRAKIMQNMCHAVLNETGHGNDPMLKVALELEKIALNDEYFINR
CEEEEECCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHCCCCCEEECC
KLYPNVDFYSGLTLKAMGFPTSMFTVLFAVARTVGWISQWSEMIEDPQQKIGRPRQLYTG
CCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHCC
KTRRDYVDMAQRA
CCHHHHHHHHHCC
>Mature Secondary Structure
MNATSTKNATLTVDDKSYEFPVLSGTVGPDVVDIGKLYAQTGMFTYDPAFTSTASCQSKI
CCCCCCCCCEEEECCCCCCCCEEECCCCCCHHHHHHHHHHCCCEEECCCCCCCCCCCCEE
TYIDGDEGVLEYRGYPIEQLAEKGDFIETCYLLLYGELPTKSQKHDFEERVTHHTMVHEQ
EEEECCCCEEEECCCCHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHH
MTRFFQSFRRDAHPMAVMVAAVGALAAFYHDSTDINDPKQRMIASMRMVAKMPTLAAMAY
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHH
KYTIGQPFIYPKNSLNFAENFLHMCFAVPCEDYDINPVLADALDKIFILHADHEQNASTS
HHCCCCCEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHCEEEEEECCCCCCCCCC
TVRIAGSSGANPFACIAAGIACLWGPAHGGANEAALNMLYEIGTVDRIPEFIDKVKDKNS
EEEEECCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCC
DVRLMGFGHRVYKNYDPRAKIMQNMCHAVLNETGHGNDPMLKVALELEKIALNDEYFINR
CEEEEECCHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHHHCCCCCEEECC
KLYPNVDFYSGLTLKAMGFPTSMFTVLFAVARTVGWISQWSEMIEDPQQKIGRPRQLYTG
CCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHCC
KTRRDYVDMAQRA
CCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]