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The map label for this gene is gltX [H]

Identifier: 75676035

GI number: 75676035

Start: 2021064

End: 2022554

Strand: Direct

Name: gltX [H]

Synonym: Nwi_1844

Alternate gene names: 75676035

Gene position: 2021064-2022554 (Clockwise)

Preceding gene: 75676032

Following gene: 75676036

Centisome position: 59.41

GC content: 62.51

Gene sequence:

>1491_bases
ATGCGAATCCCGGGCGTGATAAGCACCCCCGCCGATACCATCCTCCGAAGTGTCAGATCCGCCATAATGACAACACCCGT
CGTTACACGCTTTGCGCCATCCCCGACCGGCTTTCTTCACATCGGCGGCGGCCGGACCGCGCTTTTCAATTGGCTCTATG
CGCGCAAGCACGGAGGCAGGATGCTGCTGCGGATCGAGGATACCGATCGCGCACGGTCCACGCCGGAGGCGATCGACGCG
ATCCTTGACGGGCTGAAGTGGCTGGGAATCGAGTGGGACGACGACGTCGTGTACCAGTTTTCCCGCGTCGCGCGGCATCG
CGAGATCGCCGAGCAGTTGCTCGCCGAAGGCAAGGCCTACCGATGCTATGCGACTACGGAAGAGCTTTCCGAGATGCGCG
AGAAGGCGCGGGCCGAAGGTCGCGCCAAGCTTTACGACGGCCGCTGGCGCGATCGCGATGCGTCCGAAGCGCCGCAGGGC
GTGAAGCCAACCATCCGCCTCAAGGCCCCGCTGACCGGTGAAACCGTGATCGAGGATCAGGTTCAGGGGCGCATCGTCTG
GCAGAACGAGAACCTCGATGATCTCGTGCTGCTGCGCGGCGACGGCACGCCGACCTACATGCTCGCCGTCGTGGTCGATG
ACCACGACATGGGCGTGACGCACATCATCCGCGGCGATGACCACCTCATCAATGCGGCCCGCCAGAAGCAGATTTACGAT
ACTCTAGGATGGGCGCTGCCGACGATGGCCCACATTCCGCTTATCCATGGACCCGACGGCTCGAAGCTTTCGAAACGTCA
CGGCGCCCTGGGAGTCGATGCCTATCGAGCGATGGGATATCTGCCGGCGGCGCTTCGCAATTATCTGGTTCGGCTCGGCT
GGAGTCATGGCGATCAGGAGATCTTCTCCACGCAGGAAATGATCGACGCGTTCGACCTGCCCGCCATCGGGCGCTCCGCC
GCGCGTTTCGACTTTGCCAAGCTCGAGAACCTCAACGGCCATTATATCCGCCATTGCGACGATGCCGCACTGATGACGCT
GTTCGAGAATGCGCTTGATTTCGTTCCGGGCGGAGCGGACCTCAAGCCAAAACTCAACGATGTCACACGGGCGCAACTCC
TGCGCGCGATGCCGGGCCTCAAGGAGCGGGCCAAAACCCTAATCGAGCTGATCGAGGGCGCGCGATTCATCTTCGCCGAT
CGGCCTTTGCCGATAGAAGCCAAAGCCGCCGCATTGCTGACGCCCGAGACACGCGCGCTGATCGACAGGCTTCGCGCGGC
CCTTGAATCCGTGACGTCATGGAACGCGGAGACGACCGAAGCGGCGATGCGGACTTTTGCGGAACAGAACAATCTCAAAC
TCGGCGCCATCGCGCAGCCGCTTCGAATCGCCCTGACCGGTCGCACGACATCGCCCGGCATATTCGACGTTCTTGCCGCG
CTCGGAAAAGAGACGTGCCTGGCCCGGCTCGGCGATCAAGGCTCACGATAG

Upstream 100 bases:

>100_bases
AGCCTGGAACAGCGCCATTCCAAAATCCCTCATGGAAATAGAAACCGACAAAACAGCCCTTCGCTACATTGACGCCACGA
AGCAGGCCAGCTAGCCGTGC

Downstream 100 bases:

>100_bases
CGACCGCCATCCAGATTTGGGCGATCTTGCGGTGCAAACACCAATACGATACCCCTTTGCGCGGCGCTTTTGAATGACCG
GCTGGTTTCTCTCGTCGAGA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 496; Mature: 496

Protein sequence:

>496_residues
MRIPGVISTPADTILRSVRSAIMTTPVVTRFAPSPTGFLHIGGGRTALFNWLYARKHGGRMLLRIEDTDRARSTPEAIDA
ILDGLKWLGIEWDDDVVYQFSRVARHREIAEQLLAEGKAYRCYATTEELSEMREKARAEGRAKLYDGRWRDRDASEAPQG
VKPTIRLKAPLTGETVIEDQVQGRIVWQNENLDDLVLLRGDGTPTYMLAVVVDDHDMGVTHIIRGDDHLINAARQKQIYD
TLGWALPTMAHIPLIHGPDGSKLSKRHGALGVDAYRAMGYLPAALRNYLVRLGWSHGDQEIFSTQEMIDAFDLPAIGRSA
ARFDFAKLENLNGHYIRHCDDAALMTLFENALDFVPGGADLKPKLNDVTRAQLLRAMPGLKERAKTLIELIEGARFIFAD
RPLPIEAKAAALLTPETRALIDRLRAALESVTSWNAETTEAAMRTFAEQNNLKLGAIAQPLRIALTGRTTSPGIFDVLAA
LGKETCLARLGDQGSR

Sequences:

>Translated_496_residues
MRIPGVISTPADTILRSVRSAIMTTPVVTRFAPSPTGFLHIGGGRTALFNWLYARKHGGRMLLRIEDTDRARSTPEAIDA
ILDGLKWLGIEWDDDVVYQFSRVARHREIAEQLLAEGKAYRCYATTEELSEMREKARAEGRAKLYDGRWRDRDASEAPQG
VKPTIRLKAPLTGETVIEDQVQGRIVWQNENLDDLVLLRGDGTPTYMLAVVVDDHDMGVTHIIRGDDHLINAARQKQIYD
TLGWALPTMAHIPLIHGPDGSKLSKRHGALGVDAYRAMGYLPAALRNYLVRLGWSHGDQEIFSTQEMIDAFDLPAIGRSA
ARFDFAKLENLNGHYIRHCDDAALMTLFENALDFVPGGADLKPKLNDVTRAQLLRAMPGLKERAKTLIELIEGARFIFAD
RPLPIEAKAAALLTPETRALIDRLRAALESVTSWNAETTEAAMRTFAEQNNLKLGAIAQPLRIALTGRTTSPGIFDVLAA
LGKETCLARLGDQGSR
>Mature_496_residues
MRIPGVISTPADTILRSVRSAIMTTPVVTRFAPSPTGFLHIGGGRTALFNWLYARKHGGRMLLRIEDTDRARSTPEAIDA
ILDGLKWLGIEWDDDVVYQFSRVARHREIAEQLLAEGKAYRCYATTEELSEMREKARAEGRAKLYDGRWRDRDASEAPQG
VKPTIRLKAPLTGETVIEDQVQGRIVWQNENLDDLVLLRGDGTPTYMLAVVVDDHDMGVTHIIRGDDHLINAARQKQIYD
TLGWALPTMAHIPLIHGPDGSKLSKRHGALGVDAYRAMGYLPAALRNYLVRLGWSHGDQEIFSTQEMIDAFDLPAIGRSA
ARFDFAKLENLNGHYIRHCDDAALMTLFENALDFVPGGADLKPKLNDVTRAQLLRAMPGLKERAKTLIELIEGARFIFAD
RPLPIEAKAAALLTPETRALIDRLRAALESVTSWNAETTEAAMRTFAEQNNLKLGAIAQPLRIALTGRTTSPGIFDVLAA
LGKETCLARLGDQGSR

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=497, Percent_Identity=31.1871227364185, Blast_Score=215, Evalue=6e-56,
Organism=Homo sapiens, GI4826960, Length=156, Percent_Identity=32.0512820512821, Blast_Score=72, Evalue=2e-12,
Organism=Escherichia coli, GI1788743, Length=468, Percent_Identity=43.5897435897436, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI226510925, Length=247, Percent_Identity=39.6761133603239, Blast_Score=153, Evalue=2e-38,
Organism=Escherichia coli, GI1786895, Length=132, Percent_Identity=31.8181818181818, Blast_Score=75, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI71993394, Length=491, Percent_Identity=30.9572301425662, Blast_Score=197, Evalue=1e-50,
Organism=Caenorhabditis elegans, GI17543332, Length=197, Percent_Identity=31.4720812182741, Blast_Score=70, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6324540, Length=493, Percent_Identity=31.6430020283976, Blast_Score=229, Evalue=7e-61,
Organism=Saccharomyces cerevisiae, GI6321192, Length=212, Percent_Identity=30.188679245283, Blast_Score=88, Evalue=3e-18,
Organism=Saccharomyces cerevisiae, GI6324742, Length=108, Percent_Identity=37.037037037037, Blast_Score=81, Evalue=3e-16,
Organism=Drosophila melanogaster, GI24665321, Length=488, Percent_Identity=31.7622950819672, Blast_Score=241, Evalue=1e-63,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 54959; Mature: 54959

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIPGVISTPADTILRSVRSAIMTTPVVTRFAPSPTGFLHIGGGRTALFNWLYARKHGGR
CCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCEEEECCCHHHHHHHHHHHCCCCE
MLLRIEDTDRARSTPEAIDAILDGLKWLGIEWDDDVVYQFSRVARHREIAEQLLAEGKAY
EEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
RCYATTEELSEMREKARAEGRAKLYDGRWRDRDASEAPQGVKPTIRLKAPLTGETVIEDQ
EEEECHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHC
VQGRIVWQNENLDDLVLLRGDGTPTYMLAVVVDDHDMGVTHIIRGDDHLINAARQKQIYD
CCCEEEEECCCCCEEEEEECCCCCEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHH
TLGWALPTMAHIPLIHGPDGSKLSKRHGALGVDAYRAMGYLPAALRNYLVRLGWSHGDQE
HHHHHHHHHHHCCEEECCCCCHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCHH
IFSTQEMIDAFDLPAIGRSAARFDFAKLENLNGHYIRHCDDAALMTLFENALDFVPGGAD
HHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCC
LKPKLNDVTRAQLLRAMPGLKERAKTLIELIEGARFIFADRPLPIEAKAAALLTPETRAL
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHEEECCHHHHH
IDRLRAALESVTSWNAETTEAAMRTFAEQNNLKLGAIAQPLRIALTGRTTSPGIFDVLAA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCEEEEEECCCCCCCHHHHHHH
LGKETCLARLGDQGSR
HCHHHHHHHHCCCCCC
>Mature Secondary Structure
MRIPGVISTPADTILRSVRSAIMTTPVVTRFAPSPTGFLHIGGGRTALFNWLYARKHGGR
CCCCCCCCCCHHHHHHHHHHHHHHCHHHHHCCCCCCCEEEECCCHHHHHHHHHHHCCCCE
MLLRIEDTDRARSTPEAIDAILDGLKWLGIEWDDDVVYQFSRVARHREIAEQLLAEGKAY
EEEEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCE
RCYATTEELSEMREKARAEGRAKLYDGRWRDRDASEAPQGVKPTIRLKAPLTGETVIEDQ
EEEECHHHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCEEEEECCCCCCHHHHHC
VQGRIVWQNENLDDLVLLRGDGTPTYMLAVVVDDHDMGVTHIIRGDDHLINAARQKQIYD
CCCEEEEECCCCCEEEEEECCCCCEEEEEEEEECCCCCEEEEECCCHHHHHHHHHHHHHH
TLGWALPTMAHIPLIHGPDGSKLSKRHGALGVDAYRAMGYLPAALRNYLVRLGWSHGDQE
HHHHHHHHHHHCCEEECCCCCHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHCCCCCCHH
IFSTQEMIDAFDLPAIGRSAARFDFAKLENLNGHYIRHCDDAALMTLFENALDFVPGGAD
HHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHCCCCCC
LKPKLNDVTRAQLLRAMPGLKERAKTLIELIEGARFIFADRPLPIEAKAAALLTPETRAL
CCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHEEECCHHHHH
IDRLRAALESVTSWNAETTEAAMRTFAEQNNLKLGAIAQPLRIALTGRTTSPGIFDVLAA
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCEEEEECCCEEEEEECCCCCCCHHHHHHH
LGKETCLARLGDQGSR
HCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA