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The map label for this gene is lexA

Identifier: 75676032

GI number: 75676032

Start: 2015400

End: 2016101

Strand: Direct

Name: lexA

Synonym: Nwi_1841

Alternate gene names: 75676032

Gene position: 2015400-2016101 (Clockwise)

Preceding gene: 75676031

Following gene: 75676035

Centisome position: 59.24

GC content: 65.1

Gene sequence:

>702_bases
ATGCTCACGCGCAAACAGTACGAACTCCTGCGTTTTATCAACGAACGGCTGAAGGAGTCCGGTGTGCCGCCTTCGTTCGA
TGAAATGAAGGACGCGCTCGACCTGCGCTCGAAGTCCGGCATCCATCGCCTGATCACCGCCCTGGAGGAACGGGGCTTCA
TCCGCCGCCTGCCCAACCGCGCACGCGCCATTGAAGTCATCAAGCTGCCGGAACTGGCCGCAAGCGGCGGCGGACGTCGC
GGTTTCACACCGAGCGTCATAGAAGGCAATCTCGGCAAGGTTCGCCGCGGCGGCGGTGGCGGCGCTGACGAGGCCGAGCG
TCCGGTCGCCGTTCCGGTGATGGGACGGATCGCAGCCGGCACGCCGATCGAGGCGTTGCAGACCTGCAGTCACACAATCA
GCCTGCCGCCCGACATGCTCGGCGCCGGCGAGCACTACGCGCTCGAGGTGCGCGGCGATTCCATGGTCGATGCCGGCATC
CTCGATGGCGATATGGCGTTGATCCAGCGTAATCCGACCGCTGACACCGGCGACATCGTCGTCGCCCTGATCGACGACGA
GGAAGCGACGTTGAAACGCTTCCGCCGCCGCGGCGCTTCGATCGCGCTGGAGCCGGCGAACGCGGCCTATGAAGTTCGCA
TTCTTCCGCCCAACCGCGTCCAGATCCAGGGCCGGCTGGTCGGCATTTACCGCAAGTACTAG

Upstream 100 bases:

>100_bases
TTTGTTTCGAGTTCTCGTGCCGCGGAGTTGGCGCATAGGGCTGTAAGGCCCGATGTCGTCCGTCATCGGCAGGAACCATC
AACTAGGGATTGGGTCGGGA

Downstream 100 bases:

>100_bases
GAATCGTTCAGGTGAATAGCGGACAACATGTCGGTGATGAACCGGCCATCAAGGAAGCAAAAGAGATTTACGAGAGGTTC
CGCCGCCGCATTGTAGAGGA

Product: LexA repressor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MLTRKQYELLRFINERLKESGVPPSFDEMKDALDLRSKSGIHRLITALEERGFIRRLPNRARAIEVIKLPELAASGGGRR
GFTPSVIEGNLGKVRRGGGGGADEAERPVAVPVMGRIAAGTPIEALQTCSHTISLPPDMLGAGEHYALEVRGDSMVDAGI
LDGDMALIQRNPTADTGDIVVALIDDEEATLKRFRRRGASIALEPANAAYEVRILPPNRVQIQGRLVGIYRKY

Sequences:

>Translated_233_residues
MLTRKQYELLRFINERLKESGVPPSFDEMKDALDLRSKSGIHRLITALEERGFIRRLPNRARAIEVIKLPELAASGGGRR
GFTPSVIEGNLGKVRRGGGGGADEAERPVAVPVMGRIAAGTPIEALQTCSHTISLPPDMLGAGEHYALEVRGDSMVDAGI
LDGDMALIQRNPTADTGDIVVALIDDEEATLKRFRRRGASIALEPANAAYEVRILPPNRVQIQGRLVGIYRKY
>Mature_233_residues
MLTRKQYELLRFINERLKESGVPPSFDEMKDALDLRSKSGIHRLITALEERGFIRRLPNRARAIEVIKLPELAASGGGRR
GFTPSVIEGNLGKVRRGGGGGADEAERPVAVPVMGRIAAGTPIEALQTCSHTISLPPDMLGAGEHYALEVRGDSMVDAGI
LDGDMALIQRNPTADTGDIVVALIDDEEATLKRFRRRGASIALEPANAAYEVRILPPNRVQIQGRLVGIYRKY

Specific function: Represses a number of genes involved in the response to DNA damage (SOS response), including recA and lexA. In the presence of single-stranded DNA, recA interacts with lexA causing an autocatalytic cleavage which disrupts the DNA-binding part of lexA, lea

COG id: COG1974

COG function: function code KT; SOS-response transcriptional repressors (RecA-mediated autopeptidases)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S24 family

Homologues:

Organism=Escherichia coli, GI1790476, Length=233, Percent_Identity=26.6094420600858, Blast_Score=87, Evalue=7e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LEXA_NITWN (Q3SRJ0)

Other databases:

- EMBL:   CP000115
- RefSeq:   YP_318453.1
- ProteinModelPortal:   Q3SRJ0
- SMR:   Q3SRJ0
- STRING:   Q3SRJ0
- MEROPS:   S24.001
- GeneID:   3674766
- GenomeReviews:   CP000115_GR
- KEGG:   nwi:Nwi_1841
- NMPDR:   fig|323098.3.peg.1895
- eggNOG:   COG1974
- HOGENOM:   HBG679610
- OMA:   LMFIHER
- PhylomeDB:   Q3SRJ0
- ProtClustDB:   PRK00215
- BioCyc:   NWIN323098:NWI_1841-MONOMER
- GO:   GO:0006508
- GO:   GO:0006350
- HAMAP:   MF_00015
- InterPro:   IPR006199
- InterPro:   IPR006200
- InterPro:   IPR006197
- InterPro:   IPR019759
- InterPro:   IPR015927
- InterPro:   IPR011056
- InterPro:   IPR011991
- Gene3D:   G3DSA:2.10.109.10
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00726
- TIGRFAMs:   TIGR00498

Pfam domain/function: PF01726 LexA_DNA_bind; PF00717 Peptidase_S24; SSF51306 Pept_S24_S26_C

EC number: =3.4.21.88

Molecular weight: Translated: 25404; Mature: 25404

Theoretical pI: Translated: 9.43; Mature: 9.43

Prosite motif: NA

Important sites: ACT_SITE 154-154 ACT_SITE 192-192

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTRKQYELLRFINERLKESGVPPSFDEMKDALDLRSKSGIHRLITALEERGFIRRLPNR
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHCCCH
ARAIEVIKLPELAASGGGRRGFTPSVIEGNLGKVRRGGGGGADEAERPVAVPVMGRIAAG
HHEEEEEECCHHHHCCCCCCCCCCHHHCCCCCCEECCCCCCCCCCCCCEECCCCCHHCCC
TPIEALQTCSHTISLPPDMLGAGEHYALEVRGDSMVDAGILDGDMALIQRNPTADTGDIV
CCHHHHHHHHHCCCCCHHHHCCCCEEEEEECCCCEEECCCCCCCEEEEECCCCCCCCCEE
VALIDDEEATLKRFRRRGASIALEPANAAYEVRILPPNRVQIQGRLVGIYRKY
EEEECCCHHHHHHHHHCCCCEEEECCCCEEEEEEECCCCEEEEEEEEEEEECC
>Mature Secondary Structure
MLTRKQYELLRFINERLKESGVPPSFDEMKDALDLRSKSGIHRLITALEERGFIRRLPNR
CCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHCCCH
ARAIEVIKLPELAASGGGRRGFTPSVIEGNLGKVRRGGGGGADEAERPVAVPVMGRIAAG
HHEEEEEECCHHHHCCCCCCCCCCHHHCCCCCCEECCCCCCCCCCCCCEECCCCCHHCCC
TPIEALQTCSHTISLPPDMLGAGEHYALEVRGDSMVDAGILDGDMALIQRNPTADTGDIV
CCHHHHHHHHHCCCCCHHHHCCCCEEEEEECCCCEEECCCCCCCEEEEECCCCCCCCCEE
VALIDDEEATLKRFRRRGASIALEPANAAYEVRILPPNRVQIQGRLVGIYRKY
EEEECCCHHHHHHHHHCCCCEEEECCCCEEEEEEECCCCEEEEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA