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The map label for this gene is lipB

Identifier: 75675923

GI number: 75675923

Start: 1890841

End: 1891536

Strand: Direct

Name: lipB

Synonym: Nwi_1731

Alternate gene names: 75675923

Gene position: 1890841-1891536 (Clockwise)

Preceding gene: 75675921

Following gene: 75675930

Centisome position: 55.58

GC content: 65.95

Gene sequence:

>696_bases
ATGATTAACAGTCGCCAGACCCTCGATTTGAAGCTGTTCGCGGCCTCGCCCGGCGAGCCCGTGGACTGGCGGCGGTCGGA
TCAACCGGTGCCGTATCCGGGAGCGGTCGACGCCATGGAAAGGCGGGCCGCGGCGATCGCCTCTAGCGAGGCCGCGGAGC
AGGTCTGGCTGCTGGAGCACCCGCCGCTTTACACCTGCGGCACCAGCGGACGCGAAGCCGATCTGGTGAAGGCCCGCTTT
CCGCTATTTTCGACCGGCCGTGGCGGCCAGATCACCTATCATGGCCCCGGCCAGCGCATCGCCTACGTCATGCTGGATTT
GAAGCGCCGCCGTCCCGACGTGCGGGAGTTCATTGCCTGCCTGGAGGAATGGATCATCCGCACGCTCGACGCCTTCGGCG
TCAAGGGAGAACGGCGCGAGGATCGGGTCGGCGTCTGGGTGGCGCGACCGGACAAGGGGCCGGGCCATGAGGACAAGATC
GCGGCGATCGGCGTGCGGCTGCGCCGCTGGGTATCGTTCCACGGCGTCTCGCTCAATGTCGATCCCGATCTCAGCCATTT
CGCCGACATCGTGCCCTGCGGCGTCTCCGACCCGCGTTACGGCGTGACGAGCCTCGCGGACCTTGGTTTGCGTGTCAGGC
TGACCGATATCGATCAGGCATTGAAGCGCACCTTCAGCGAAGTGTTCGGTTCCTGA

Upstream 100 bases:

>100_bases
CCATGAGATCGACCGATACTTTGTCGAGCGTCGCCACGAAATTCAGACCCGGAGCCATGCCAATTGCTACGGTTCCACAC
CACCACATGATGGTTAGCCA

Downstream 100 bases:

>100_bases
GAATCCCGCGTTTATTTGACGGGATCGATGATATTGATCCCGGTAGCGGCAATCAAAGCACGTGTCTAAAACCTCGCCCG
CGCGCTCAGCAGGAAATTAC

Product: lipoyltransferase

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MINSRQTLDLKLFAASPGEPVDWRRSDQPVPYPGAVDAMERRAAAIASSEAAEQVWLLEHPPLYTCGTSGREADLVKARF
PLFSTGRGGQITYHGPGQRIAYVMLDLKRRRPDVREFIACLEEWIIRTLDAFGVKGERREDRVGVWVARPDKGPGHEDKI
AAIGVRLRRWVSFHGVSLNVDPDLSHFADIVPCGVSDPRYGVTSLADLGLRVRLTDIDQALKRTFSEVFGS

Sequences:

>Translated_231_residues
MINSRQTLDLKLFAASPGEPVDWRRSDQPVPYPGAVDAMERRAAAIASSEAAEQVWLLEHPPLYTCGTSGREADLVKARF
PLFSTGRGGQITYHGPGQRIAYVMLDLKRRRPDVREFIACLEEWIIRTLDAFGVKGERREDRVGVWVARPDKGPGHEDKI
AAIGVRLRRWVSFHGVSLNVDPDLSHFADIVPCGVSDPRYGVTSLADLGLRVRLTDIDQALKRTFSEVFGS
>Mature_231_residues
MINSRQTLDLKLFAASPGEPVDWRRSDQPVPYPGAVDAMERRAAAIASSEAAEQVWLLEHPPLYTCGTSGREADLVKARF
PLFSTGRGGQITYHGPGQRIAYVMLDLKRRRPDVREFIACLEEWIIRTLDAFGVKGERREDRVGVWVARPDKGPGHEDKI
AAIGVRLRRWVSFHGVSLNVDPDLSHFADIVPCGVSDPRYGVTSLADLGLRVRLTDIDQALKRTFSEVFGS

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=193, Percent_Identity=33.160621761658, Blast_Score=75, Evalue=4e-14,
Organism=Escherichia coli, GI87081767, Length=145, Percent_Identity=44.1379310344828, Blast_Score=135, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6323268, Length=127, Percent_Identity=34.6456692913386, Blast_Score=81, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21355483, Length=180, Percent_Identity=35.5555555555556, Blast_Score=105, Evalue=4e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_NITWN (Q3SRU9)

Other databases:

- EMBL:   CP000115
- RefSeq:   YP_318344.1
- ProteinModelPortal:   Q3SRU9
- SMR:   Q3SRU9
- STRING:   Q3SRU9
- GeneID:   3675136
- GenomeReviews:   CP000115_GR
- KEGG:   nwi:Nwi_1731
- NMPDR:   fig|323098.3.peg.1479
- eggNOG:   COG0321
- HOGENOM:   HBG716160
- OMA:   DLKRRRP
- PhylomeDB:   Q3SRU9
- ProtClustDB:   CLSK2516673
- BioCyc:   NWIN323098:NWI_1731-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 25738; Mature: 25738

Theoretical pI: Translated: 8.08; Mature: 8.08

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 193-193

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINSRQTLDLKLFAASPGEPVDWRRSDQPVPYPGAVDAMERRAAAIASSEAAEQVWLLEH
CCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEECC
PPLYTCGTSGREADLVKARFPLFSTGRGGQITYHGPGQRIAYVMLDLKRRRPDVREFIAC
CCEEECCCCCCCCCHHHCCCCEEECCCCCEEEECCCCCEEHHHHHHHHHCCCCHHHHHHH
LEEWIIRTLDAFGVKGERREDRVGVWVARPDKGPGHEDKIAAIGVRLRRWVSFHGVSLNV
HHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEC
DPDLSHFADIVPCGVSDPRYGVTSLADLGLRVRLTDIDQALKRTFSEVFGS
CCCHHHHHHHCCCCCCCCCCCHHHHHHCCEEEEEHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MINSRQTLDLKLFAASPGEPVDWRRSDQPVPYPGAVDAMERRAAAIASSEAAEQVWLLEH
CCCCCCEEEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHEEEEECC
PPLYTCGTSGREADLVKARFPLFSTGRGGQITYHGPGQRIAYVMLDLKRRRPDVREFIAC
CCEEECCCCCCCCCHHHCCCCEEECCCCCEEEECCCCCEEHHHHHHHHHCCCCHHHHHHH
LEEWIIRTLDAFGVKGERREDRVGVWVARPDKGPGHEDKIAAIGVRLRRWVSFHGVSLNV
HHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEC
DPDLSHFADIVPCGVSDPRYGVTSLADLGLRVRLTDIDQALKRTFSEVFGS
CCCHHHHHHHCCCCCCCCCCCHHHHHHCCEEEEEHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA