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The map label for this gene is ksgA

Identifier: 75675874

GI number: 75675874

Start: 1835075

End: 1835938

Strand: Direct

Name: ksgA

Synonym: Nwi_1682

Alternate gene names: 75675874

Gene position: 1835075-1835938 (Clockwise)

Preceding gene: 75675873

Following gene: 75675881

Centisome position: 53.94

GC content: 63.31

Gene sequence:

>864_bases
ATGAGCATGATCGATGACCTTCCGCCGCTGCGCGACGTCATCAAGCGTCACGCTTTGTCGGCGCGAAAATCGTTGGGACA
GAATTTTCTGCTCGATCTGAATCTCACATCCAGGATCGCGCGCGCCGCCGGTCCGCTTCAGGATGCGACAGTCGTCGAGA
TCGGTCCCGGCCCCGGCGGATTGACACGAGCGTTGCTTGCGGTCGGCGCGAAGCATGTTATCGCCATTGAGCGTGACGAA
CGCGCGCTCGGCGCGCTGGAAGAAATCTCGGATCGCTATCCCGGCCGTCTCACGATCATCAACGCAGATGCGACGGACTT
CGATCCGCGGCCCCTGCTCGGCACGACGCGCGCAAAGATCGTCGCCAACCTGCCTTACAACATCGCCACCGCGCTTTTGA
TCCGATGGCTCAGCATCGAGCCTTGGCCACCCTGGTACGATGTCATGGTGCTGATGTTTCAGCGCGAGGTGGCCGAGCGG
ATCGTTGCCCGTGAAAACGAAGACGCCTATGGCCGTCTCGGCGTTCTGTCGAACTGGCGGGCCGAGACCAAAATCCTCTT
CGACATCTCGCCCGCCGCCTTCGTTCCGCAACCCCAGGTCACGTCTTCCGTGGTGCGGCTGATACCGCGCTACAACCCCG
AGCCCTGCGACCGCCGCAGCCTGGAGCAAGTCGCTGCCGCCGCGTTCGGGCATCGCCGGAAAATGCTGCGGCAGAGTCTC
AAATCGTTACCCGCCGACCCGGCGTCGCTCGCGGCCGCGGCCGGCATCGATCCCACGCGGCGCGCGGAGACGGTTTCGAT
ATCCGGCTTTGCGGCCATGGCGCGTGAGTTGGCGAGCATCGTTGGAAGCGGCAAAAGACTCTAA

Upstream 100 bases:

>100_bases
GCACCGCGTTCGACATAGCCGGCACGGGGCGCGCCAGTCCATCGAGTCTGATCGCCGCGCTGCGGCTCGCCGCGCGGATG
GCAGGTCCGACGCCTTCGTC

Downstream 100 bases:

>100_bases
GTTTCGAGTTGCCGCTGTAGTTCTCGTACGAAAGCGGTCAATCCGGTCCGGCGTTCGCGCTTGAGCCGCTCAGCCTTCAG
GATAGACTGCACCTCGGCGA

Product: dimethyladenosine transferase

Products: NA

Alternate protein names: 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))-dimethyltransferase; 16S rRNA dimethyladenosine transferase; 16S rRNA dimethylase; S-adenosylmethionine-6-N', N'-adenosyl(rRNA) dimethyltransferase

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MSMIDDLPPLRDVIKRHALSARKSLGQNFLLDLNLTSRIARAAGPLQDATVVEIGPGPGGLTRALLAVGAKHVIAIERDE
RALGALEEISDRYPGRLTIINADATDFDPRPLLGTTRAKIVANLPYNIATALLIRWLSIEPWPPWYDVMVLMFQREVAER
IVARENEDAYGRLGVLSNWRAETKILFDISPAAFVPQPQVTSSVVRLIPRYNPEPCDRRSLEQVAAAAFGHRRKMLRQSL
KSLPADPASLAAAAGIDPTRRAETVSISGFAAMARELASIVGSGKRL

Sequences:

>Translated_287_residues
MSMIDDLPPLRDVIKRHALSARKSLGQNFLLDLNLTSRIARAAGPLQDATVVEIGPGPGGLTRALLAVGAKHVIAIERDE
RALGALEEISDRYPGRLTIINADATDFDPRPLLGTTRAKIVANLPYNIATALLIRWLSIEPWPPWYDVMVLMFQREVAER
IVARENEDAYGRLGVLSNWRAETKILFDISPAAFVPQPQVTSSVVRLIPRYNPEPCDRRSLEQVAAAAFGHRRKMLRQSL
KSLPADPASLAAAAGIDPTRRAETVSISGFAAMARELASIVGSGKRL
>Mature_286_residues
SMIDDLPPLRDVIKRHALSARKSLGQNFLLDLNLTSRIARAAGPLQDATVVEIGPGPGGLTRALLAVGAKHVIAIERDER
ALGALEEISDRYPGRLTIINADATDFDPRPLLGTTRAKIVANLPYNIATALLIRWLSIEPWPPWYDVMVLMFQREVAERI
VARENEDAYGRLGVLSNWRAETKILFDISPAAFVPQPQVTSSVVRLIPRYNPEPCDRRSLEQVAAAAFGHRRKMLRQSLK
SLPADPASLAAAAGIDPTRRAETVSISGFAAMARELASIVGSGKRL

Specific function: Specifically dimethylates two adjacent adenosines (A1518 and A1519) in the loop of a conserved hairpin near the 3'-end of 16S rRNA in the 30S particle. May play a critical role in biogenesis of 30S subunits

COG id: COG0030

COG function: function code J; Dimethyladenosine transferase (rRNA methylation)

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. rRNA adenine N(6)-methyltransferase family. RsmA subfamily

Homologues:

Organism=Homo sapiens, GI156415992, Length=292, Percent_Identity=35.6164383561644, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI7657198, Length=228, Percent_Identity=32.4561403508772, Blast_Score=95, Evalue=6e-20,
Organism=Escherichia coli, GI1786236, Length=265, Percent_Identity=36.9811320754717, Blast_Score=140, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI25141369, Length=295, Percent_Identity=33.5593220338983, Blast_Score=154, Evalue=7e-38,
Organism=Caenorhabditis elegans, GI25146882, Length=216, Percent_Identity=32.8703703703704, Blast_Score=95, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6324989, Length=212, Percent_Identity=33.0188679245283, Blast_Score=78, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21357273, Length=259, Percent_Identity=35.1351351351351, Blast_Score=125, Evalue=2e-29,
Organism=Drosophila melanogaster, GI21358017, Length=231, Percent_Identity=32.034632034632, Blast_Score=96, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RSMA_NITWN (Q3SRZ8)

Other databases:

- EMBL:   CP000115
- RefSeq:   YP_318295.1
- ProteinModelPortal:   Q3SRZ8
- STRING:   Q3SRZ8
- GeneID:   3674714
- GenomeReviews:   CP000115_GR
- KEGG:   nwi:Nwi_1682
- NMPDR:   fig|323098.3.peg.1303
- eggNOG:   COG0030
- HOGENOM:   HBG319664
- OMA:   GVLCGWR
- PhylomeDB:   Q3SRZ8
- ProtClustDB:   PRK00274
- BioCyc:   NWIN323098:NWI_1682-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00607
- InterPro:   IPR023165
- InterPro:   IPR020596
- InterPro:   IPR001737
- InterPro:   IPR020598
- InterPro:   IPR011530
- Gene3D:   G3DSA:1.10.8.100
- PANTHER:   PTHR11727
- SMART:   SM00650
- TIGRFAMs:   TIGR00755

Pfam domain/function: PF00398 RrnaAD

EC number: =2.1.1.182

Molecular weight: Translated: 31398; Mature: 31267

Theoretical pI: Translated: 10.12; Mature: 10.12

Prosite motif: PS01131 RRNA_A_DIMETH

Important sites: BINDING 28-28 BINDING 30-30 BINDING 55-55 BINDING 77-77 BINDING 103-103 BINDING 123-123

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSMIDDLPPLRDVIKRHALSARKSLGQNFLLDLNLTSRIARAAGPLQDATVVEIGPGPGG
CCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHCCCCCCCEEEEECCCCCH
LTRALLAVGAKHVIAIERDERALGALEEISDRYPGRLTIINADATDFDPRPLLGTTRAKI
HHHHHHHHCCHHEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHE
VANLPYNIATALLIRWLSIEPWPPWYDVMVLMFQREVAERIVARENEDAYGRLGVLSNWR
EECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCC
AETKILFDISPAAFVPQPQVTSSVVRLIPRYNPEPCDRRSLEQVAAAAFGHRRKMLRQSL
CCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KSLPADPASLAAAAGIDPTRRAETVSISGFAAMARELASIVGSGKRL
HHCCCCHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SMIDDLPPLRDVIKRHALSARKSLGQNFLLDLNLTSRIARAAGPLQDATVVEIGPGPGG
CCCCCCCHHHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHCCCCCCCEEEEECCCCCH
LTRALLAVGAKHVIAIERDERALGALEEISDRYPGRLTIINADATDFDPRPLLGTTRAKI
HHHHHHHHCCHHEEEEECCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCHHHE
VANLPYNIATALLIRWLSIEPWPPWYDVMVLMFQREVAERIVARENEDAYGRLGVLSNWR
EECCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCC
AETKILFDISPAAFVPQPQVTSSVVRLIPRYNPEPCDRRSLEQVAAAAFGHRRKMLRQSL
CCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KSLPADPASLAAAAGIDPTRRAETVSISGFAAMARELASIVGSGKRL
HHCCCCHHHHHHHCCCCCCCCCCEEEEHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA