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The map label for this gene is gloA [H]

Identifier: 75675803

GI number: 75675803

Start: 1758734

End: 1759183

Strand: Direct

Name: gloA [H]

Synonym: Nwi_1611

Alternate gene names: 75675803

Gene position: 1758734-1759183 (Clockwise)

Preceding gene: 75675795

Following gene: 75675804

Centisome position: 51.7

GC content: 58.89

Gene sequence:

>450_bases
ATGCGCTACCTTCATACCATGCTGCGCGTGCGCAATCTTGAGATCGCGCTGAAATTCTATGTGGACGCGTTGGGATTGAA
GGAGGTCCGGCGCGTCGACAACGACAAGGGGAAATTCACGCTGGTATTCCTGTGCGCCCCCGAGGACGAGGACCTTGTGA
GGCAGTCGAGTGGGCGTGGCGCGCCGCTGGTCGAACTGACGTACAACTGGGTCGAGGAGACCTATGGCGAGGATCGCTTC
TTTGGTCACCTTGCCTATGAGGTGGACGACATCTACGCCACGTGTGAAAGGCTGATGAGGCTCGGGATCACGATCAATCG
CCCTCCCCGCGATGGACAGATGGCGTTCATCCGCTCTCCGGACCTGCACTCGATCGAACTGCTGCAGAAGGGCAAACCTC
TTCCGGCGGCTGAACCGTGGACTTCAATGCCCAACACCGGTCATTGGTGA

Upstream 100 bases:

>100_bases
TCGCGGCCCGGAATCGGAAACCGGATACAAAATCGACCATACCGATCTGATATTGCTATGATTCTATTCACCCTCATCAT
CTCGTCAGGAGACATCACGG

Downstream 100 bases:

>100_bases
GAACCTCCGGCGACGTCGCTTGTGCCTGAATCGCGCGCGTTCGCGATGGGCAACGGGCGCGTCGTTGCGAGGCCACCTCA
GTCCCAGGCAGTTGCTCGTC

Product: glyoxalase/bleomycin resistance protein/dioxygenase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MRYLHTMLRVRNLEIALKFYVDALGLKEVRRVDNDKGKFTLVFLCAPEDEDLVRQSSGRGAPLVELTYNWVEETYGEDRF
FGHLAYEVDDIYATCERLMRLGITINRPPRDGQMAFIRSPDLHSIELLQKGKPLPAAEPWTSMPNTGHW

Sequences:

>Translated_149_residues
MRYLHTMLRVRNLEIALKFYVDALGLKEVRRVDNDKGKFTLVFLCAPEDEDLVRQSSGRGAPLVELTYNWVEETYGEDRF
FGHLAYEVDDIYATCERLMRLGITINRPPRDGQMAFIRSPDLHSIELLQKGKPLPAAEPWTSMPNTGHW
>Mature_149_residues
MRYLHTMLRVRNLEIALKFYVDALGLKEVRRVDNDKGKFTLVFLCAPEDEDLVRQSSGRGAPLVELTYNWVEETYGEDRF
FGHLAYEVDDIYATCERLMRLGITINRPPRDGQMAFIRSPDLHSIELLQKGKPLPAAEPWTSMPNTGHW

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=146, Percent_Identity=33.5616438356164, Blast_Score=80, Evalue=7e-16,
Organism=Escherichia coli, GI1787940, Length=136, Percent_Identity=36.0294117647059, Blast_Score=85, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6323639, Length=142, Percent_Identity=33.0985915492958, Blast_Score=74, Evalue=9e-15,
Organism=Drosophila melanogaster, GI20129743, Length=145, Percent_Identity=33.1034482758621, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 17241; Mature: 17241

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYLHTMLRVRNLEIALKFYVDALGLKEVRRVDNDKGKFTLVFLCAPEDEDLVRQSSGRG
CHHHHHHHHHHHHHHHEEHHHHHHCHHHHHHCCCCCCCEEEEEEECCCCHHHHHHCCCCC
APLVELTYNWVEETYGEDRFFGHLAYEVDDIYATCERLMRLGITINRPPRDGQMAFIRSP
CCEEEEHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHCCCEECCCCCCCCEEEEECC
DLHSIELLQKGKPLPAAEPWTSMPNTGHW
CCCHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRYLHTMLRVRNLEIALKFYVDALGLKEVRRVDNDKGKFTLVFLCAPEDEDLVRQSSGRG
CHHHHHHHHHHHHHHHEEHHHHHHCHHHHHHCCCCCCCEEEEEEECCCCHHHHHHCCCCC
APLVELTYNWVEETYGEDRFFGHLAYEVDDIYATCERLMRLGITINRPPRDGQMAFIRSP
CCEEEEHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHCCCEECCCCCCCCEEEEECC
DLHSIELLQKGKPLPAAEPWTSMPNTGHW
CCCHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]