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The map label for this gene is dat [H]

Identifier: 75675640

GI number: 75675640

Start: 1578806

End: 1579663

Strand: Direct

Name: dat [H]

Synonym: Nwi_1448

Alternate gene names: 75675640

Gene position: 1578806-1579663 (Clockwise)

Preceding gene: 75675639

Following gene: 75675641

Centisome position: 46.41

GC content: 63.17

Gene sequence:

>858_bases
ATGTCGCGCATCGCTTATGTCAACGGCCGGTATCGCGATCTGCGCGATGCCTGCGTCAACGTCGAGGATCGCGGCTACCA
GTTTGCCGACGGTGTTTACGAGGTTTGCGAGGTGCGCGGCGCGAGGCTGGTTGATTTTCCCCGGCACATGGCCCGGTTGC
AGCGGTCGATGCGCGAATTGCGGATCGCCGAACCGATGCCGATCACGGCGCTCGGCATCATCATGCGCGAGGTCGTGCGG
CGGAATCGCGTGACCTACGGAATCGTGTATCTGCAGGTGACTCGCGGCGTGGCCCGGCGCGATCACGCTTTTCCGCCGAA
CCCGGTCAAGCCGGGCGTCGTCGTCACGGCCCGCGCGCTGGATGACGAGAAAAAGCAGCAAGCCGCCGCCGCCGGCATTT
CTGTCATTACTCTGCCGGAGAATCGTTGGCCGCGGGTGGATATCAAGTCAGTGGCGCTGCTGCCGAATGTTTTGGCAAAG
CAGCATGCGCGGGAAAAGGGAGCCCATGAGGCGTGGTACGTCGATAGTGACGGATTCGTCACCGAAGGCGCGTCGAGCAA
CGCCTGGATCGTGACGAAGGACGGCCGGGCCGTCACCCGGTCAGTGGACCAGGGCATTCTGGCGGGCATCACGCGGGCTG
TTCTCATGGAAGTCCTGAGGGCTTTTCAGATCAGGCTTGAAGAGCGCCCGTTCACGCCGGCGGAAGCCCGCGAGGCGGCC
GAAGCGTTTGTAACCTCGGCTTCTCAGATCGTCATGCCCGTGGTCGCGATCGACGGCCAGGTGGTGGGGGATGGTCGGCC
TGGCGGTATTGCAAGGCGGCTTCGAGAGGAATTCCACCGCTTTTCAGCATTTTCCTGA

Upstream 100 bases:

>100_bases
GCGCTCGGCGTCAACTAGATTGTTGCTCTGACGCATGGTCATCCCGCGAAATCGTATTCATCTCGATCGAGAACAGGTAA
CGCTGAGGTAAACCTGCTGA

Downstream 100 bases:

>100_bases
TGGTTTTTGTGTATCCGGGTAAACCCGCGGACCCGAACCGGCTTGCGTAACATCATACTCTGCCTTCTAATCCGGGGGGC
CGGGTCCATGAGGAGGATCT

Product: D-amino acid aminotransferase

Products: NA

Alternate protein names: D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MSRIAYVNGRYRDLRDACVNVEDRGYQFADGVYEVCEVRGARLVDFPRHMARLQRSMRELRIAEPMPITALGIIMREVVR
RNRVTYGIVYLQVTRGVARRDHAFPPNPVKPGVVVTARALDDEKKQQAAAAGISVITLPENRWPRVDIKSVALLPNVLAK
QHAREKGAHEAWYVDSDGFVTEGASSNAWIVTKDGRAVTRSVDQGILAGITRAVLMEVLRAFQIRLEERPFTPAEAREAA
EAFVTSASQIVMPVVAIDGQVVGDGRPGGIARRLREEFHRFSAFS

Sequences:

>Translated_285_residues
MSRIAYVNGRYRDLRDACVNVEDRGYQFADGVYEVCEVRGARLVDFPRHMARLQRSMRELRIAEPMPITALGIIMREVVR
RNRVTYGIVYLQVTRGVARRDHAFPPNPVKPGVVVTARALDDEKKQQAAAAGISVITLPENRWPRVDIKSVALLPNVLAK
QHAREKGAHEAWYVDSDGFVTEGASSNAWIVTKDGRAVTRSVDQGILAGITRAVLMEVLRAFQIRLEERPFTPAEAREAA
EAFVTSASQIVMPVVAIDGQVVGDGRPGGIARRLREEFHRFSAFS
>Mature_284_residues
SRIAYVNGRYRDLRDACVNVEDRGYQFADGVYEVCEVRGARLVDFPRHMARLQRSMRELRIAEPMPITALGIIMREVVRR
NRVTYGIVYLQVTRGVARRDHAFPPNPVKPGVVVTARALDDEKKQQAAAAGISVITLPENRWPRVDIKSVALLPNVLAKQ
HAREKGAHEAWYVDSDGFVTEGASSNAWIVTKDGRAVTRSVDQGILAGITRAVLMEVLRAFQIRLEERPFTPAEAREAAE
AFVTSASQIVMPVVAIDGQVVGDGRPGGIARRLREEFHRFSAFS

Specific function: Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha- keto acid in

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=294, Percent_Identity=26.1904761904762, Blast_Score=84, Evalue=1e-17,
Organism=Escherichia coli, GI1787338, Length=242, Percent_Identity=27.2727272727273, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005784 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.21 [H]

Molecular weight: Translated: 31762; Mature: 31631

Theoretical pI: Translated: 10.14; Mature: 10.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRIAYVNGRYRDLRDACVNVEDRGYQFADGVYEVCEVRGARLVDFPRHMARLQRSMREL
CCCEEEECCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHH
RIAEPMPITALGIIMREVVRRNRVTYGIVYLQVTRGVARRDHAFPPNPVKPGVVVTARAL
HCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEHHHHHHCCCCCCCCCCCCCEEEEEEHH
DDEKKQQAAAAGISVITLPENRWPRVDIKSVALLPNVLAKQHAREKGAHEAWYVDSDGFV
CHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCE
TEGASSNAWIVTKDGRAVTRSVDQGILAGITRAVLMEVLRAFQIRLEERPFTPAEAREAA
ECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
EAFVTSASQIVMPVVAIDGQVVGDGRPGGIARRLREEFHRFSAFS
HHHHHHHHHHHHEEEEECCEEEECCCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SRIAYVNGRYRDLRDACVNVEDRGYQFADGVYEVCEVRGARLVDFPRHMARLQRSMREL
CCEEEECCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHCCCEEHHHHHHHHHHHHHHHHH
RIAEPMPITALGIIMREVVRRNRVTYGIVYLQVTRGVARRDHAFPPNPVKPGVVVTARAL
HCCCCCCHHHHHHHHHHHHHHCCCEEEEEEEEEHHHHHHCCCCCCCCCCCCCEEEEEEHH
DDEKKQQAAAAGISVITLPENRWPRVDIKSVALLPNVLAKQHAREKGAHEAWYVDSDGFV
CHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCE
TEGASSNAWIVTKDGRAVTRSVDQGILAGITRAVLMEVLRAFQIRLEERPFTPAEAREAA
ECCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHH
EAFVTSASQIVMPVVAIDGQVVGDGRPGGIARRLREEFHRFSAFS
HHHHHHHHHHHHEEEEECCEEEECCCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]