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The map label for this gene is trmJ [H]

Identifier: 75675487

GI number: 75675487

Start: 1429543

End: 1430754

Strand: Direct

Name: trmJ [H]

Synonym: Nwi_1295

Alternate gene names: 75675487

Gene position: 1429543-1430754 (Clockwise)

Preceding gene: 75675484

Following gene: 75675490

Centisome position: 42.02

GC content: 67.08

Gene sequence:

>1212_bases
ATGTCCGGCACCGACAGGACGAAAACCGGCATCGACGTCGTCGGTCCCACCGTAATTCTGGTCGAGCCGCAGCTTGGCGA
GAATATCGGCATGGCGGCGCGCGCCATGGGCAATTTCGCCCTGACGCGGCTGCGCATCGTTAACCCGCGCGACGGCTGGC
CCAATATCGCGGCGCAGCGCGCGGCGGCGGGCGCCGACCACATCCTCTCCCGAGTTGAACTGTTCGATACCGTGGCCGAG
GCGGTCGCCGACTGCACGCTGCTGTTCGCGACCACCGCGCGCGCCCACGATCAGGCCAAGCCCGTGGTAGCGCCGGAAGC
TGCGGCGGTCGAGGCGACCGCGCAGATCGCAGCAGGCGGCCACGTCGGCATCCTGTTCGGCCGCGAACGCGCCGGCCTTT
TGAACGAGGAGGTGGCGATGGCGGATCGCATCATCACCTATCCGGTCAATCCGGGCTTCGCCTCGCTCAACCTGGCGCAG
GCGGTGCTGCTGATTGGTTACGAATGGTTCAAGCTGGTGACGCGGGGCGAATTGCCGTTCGCGATGCCGGAGCGCTCGGA
GCGGGCCTCCCAGCACCAGATGCAGGCGTTCTTCGACAATCTCGTCGCCGAACTCGACAAGGTGGAATTCCTCCGTCCGC
CGGAAAAGCGCGACACCATGCTGGTCAACCTGCGCAATATCTTCACCCGCATGGAGCCGACCAAGCAGGACATGCACACG
CTTCATGGGGTCGTCATGGCGATCGCGGAGGGGCGCAAGGGGCCGGCCAAGGGCGGCGTGCTCGACGGCGCGCAGGCGAC
TCGGCTGCGGGCGCTTTTGGCCGAGCAGGGGCCGGGAGGAGCTTCCGCCGACAGCAGCAGCACGGTGCGCGGACTGGCGC
GGCTGCTGAGGCGAAATCCCACCGATGCCGAGCGCATCCTGTGGCAGGCGCTGACCCGCGACCGCCGCTTCGCCGGACAG
TTCAAGCGGCAGACACCGGTCGGGCGGCACATCCCGGACTTCGTGTCGTTCGTGCAGCGCGTTGCAATCGAACTTGTCAA
CGCGGACGAGAGCGACACCATCGCACGCGACCGCGCCGCGCGATCGGCATGGCTCGCCGAACGCGGCTATCGCGTGATCG
AGATGGAGGCTGCGGCGGTGGAGGCTGATCTGGCGCACGTGCTTGATGGGTTGGCGGGGAGGATGGAATCTGGAAAAGAC
CCGAAACCGTAA

Upstream 100 bases:

>100_bases
TCATTATTTGACTTGAGAAAAGCTGTGAAAGGCGCGATCGAAGAAGCGGCAGGCCGCCATTGAACCAGAATCTCAGCCTG
AACTGAAAGAACGAGGCAGG

Downstream 100 bases:

>100_bases
AATTATCGCCAAACTTTCATTGAGCAGAGTAAAGTTGAGAATTTTCAATTCACCAATGTGTTCAAACTAGGTGTTCAGTC
CCGGCATTTGATGGATTGGA

Product: RNA methyltransferase TrmH, group 1

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSGTDRTKTGIDVVGPTVILVEPQLGENIGMAARAMGNFALTRLRIVNPRDGWPNIAAQRAAAGADHILSRVELFDTVAE
AVADCTLLFATTARAHDQAKPVVAPEAAAVEATAQIAAGGHVGILFGRERAGLLNEEVAMADRIITYPVNPGFASLNLAQ
AVLLIGYEWFKLVTRGELPFAMPERSERASQHQMQAFFDNLVAELDKVEFLRPPEKRDTMLVNLRNIFTRMEPTKQDMHT
LHGVVMAIAEGRKGPAKGGVLDGAQATRLRALLAEQGPGGASADSSSTVRGLARLLRRNPTDAERILWQALTRDRRFAGQ
FKRQTPVGRHIPDFVSFVQRVAIELVNADESDTIARDRAARSAWLAERGYRVIEMEAAAVEADLAHVLDGLAGRMESGKD
PKP

Sequences:

>Translated_403_residues
MSGTDRTKTGIDVVGPTVILVEPQLGENIGMAARAMGNFALTRLRIVNPRDGWPNIAAQRAAAGADHILSRVELFDTVAE
AVADCTLLFATTARAHDQAKPVVAPEAAAVEATAQIAAGGHVGILFGRERAGLLNEEVAMADRIITYPVNPGFASLNLAQ
AVLLIGYEWFKLVTRGELPFAMPERSERASQHQMQAFFDNLVAELDKVEFLRPPEKRDTMLVNLRNIFTRMEPTKQDMHT
LHGVVMAIAEGRKGPAKGGVLDGAQATRLRALLAEQGPGGASADSSSTVRGLARLLRRNPTDAERILWQALTRDRRFAGQ
FKRQTPVGRHIPDFVSFVQRVAIELVNADESDTIARDRAARSAWLAERGYRVIEMEAAAVEADLAHVLDGLAGRMESGKD
PKP
>Mature_402_residues
SGTDRTKTGIDVVGPTVILVEPQLGENIGMAARAMGNFALTRLRIVNPRDGWPNIAAQRAAAGADHILSRVELFDTVAEA
VADCTLLFATTARAHDQAKPVVAPEAAAVEATAQIAAGGHVGILFGRERAGLLNEEVAMADRIITYPVNPGFASLNLAQA
VLLIGYEWFKLVTRGELPFAMPERSERASQHQMQAFFDNLVAELDKVEFLRPPEKRDTMLVNLRNIFTRMEPTKQDMHTL
HGVVMAIAEGRKGPAKGGVLDGAQATRLRALLAEQGPGGASADSSSTVRGLARLLRRNPTDAERILWQALTRDRRFAGQF
KRQTPVGRHIPDFVSFVQRVAIELVNADESDTIARDRAARSAWLAERGYRVIEMEAAAVEADLAHVLDGLAGRMESGKDP
KP

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=248, Percent_Identity=33.8709677419355, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1790865, Length=182, Percent_Identity=35.1648351648352, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 43762; Mature: 43630

Theoretical pI: Translated: 7.30; Mature: 7.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGTDRTKTGIDVVGPTVILVEPQLGENIGMAARAMGNFALTRLRIVNPRDGWPNIAAQR
CCCCCCCCCCCEECCCEEEEECCCCCCCCCHHHHHHHHHHEEEEEEECCCCCCCCHHHHH
AAAGADHILSRVELFDTVAEAVADCTLLFATTARAHDQAKPVVAPEAAAVEATAQIAAGG
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCC
HVGILFGRERAGLLNEEVAMADRIITYPVNPGFASLNLAQAVLLIGYEWFKLVTRGELPF
CEEEEECCCCCCCHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
AMPERSERASQHQMQAFFDNLVAELDKVEFLRPPEKRDTMLVNLRNIFTRMEPTKQDMHT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHCCCCHHHHHH
LHGVVMAIAEGRKGPAKGGVLDGAQATRLRALLAEQGPGGASADSSSTVRGLARLLRRNP
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC
TDAERILWQALTRDRRFAGQFKRQTPVGRHIPDFVSFVQRVAIELVNADESDTIARDRAA
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
RSAWLAERGYRVIEMEAAAVEADLAHVLDGLAGRMESGKDPKP
HHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SGTDRTKTGIDVVGPTVILVEPQLGENIGMAARAMGNFALTRLRIVNPRDGWPNIAAQR
CCCCCCCCCCEECCCEEEEECCCCCCCCCHHHHHHHHHHEEEEEEECCCCCCCCHHHHH
AAAGADHILSRVELFDTVAEAVADCTLLFATTARAHDQAKPVVAPEAAAVEATAQIAAGG
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCC
HVGILFGRERAGLLNEEVAMADRIITYPVNPGFASLNLAQAVLLIGYEWFKLVTRGELPF
CEEEEECCCCCCCHHHHHHHHCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
AMPERSERASQHQMQAFFDNLVAELDKVEFLRPPEKRDTMLVNLRNIFTRMEPTKQDMHT
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHCCCCHHHHHH
LHGVVMAIAEGRKGPAKGGVLDGAQATRLRALLAEQGPGGASADSSSTVRGLARLLRRNP
HHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCC
TDAERILWQALTRDRRFAGQFKRQTPVGRHIPDFVSFVQRVAIELVNADESDTIARDRAA
CHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
RSAWLAERGYRVIEMEAAAVEADLAHVLDGLAGRMESGKDPKP
HHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA