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The map label for this gene is nadE [H]

Identifier: 75675418

GI number: 75675418

Start: 1347381

End: 1349132

Strand: Direct

Name: nadE [H]

Synonym: Nwi_1225

Alternate gene names: 75675418

Gene position: 1347381-1349132 (Clockwise)

Preceding gene: 75675417

Following gene: 75675421

Centisome position: 39.6

GC content: 65.18

Gene sequence:

>1752_bases
ATGACCGAAACAACCTTCAAGATCACGCTCGGCCAACTAAACCCGACGGTCGGAGACATCGTCGGCAATGCGGCCAAGGC
CCGCGCCGCGCGCGCTCAGGCGAAGGCCGACGGCGCCGCGCTGGTGGCTCTTCCCGAACTGTTCATCGCCGGCTATCCGC
CCGAGGATCTGGTTCTGAGGCCGGCATTTCAGGCTGCCTGCCGCGAGGCGATCGAAGCTCTCGCGCGAGAGACCAGTGAC
GGCGGCCCGGCCATGCTGATCGGCACTCCATGGGTCGATGACGGCAAGCTCTACAATGCCTATGCGTTGCTGGACGAGGG
GCGGGTCGCGGCGCTTCGTTTCAAAGTCAATCTGCCGAACTACGGCGTGTTCGACGAAAAGCGCGTGTTCGCGCGGGGAC
CGGTCGGAGGCCCGTTGAAGATTCGGGGCGTGCGGATCGGCGTGCCGATCTGTGAAGATACCTGGGTTGAGGAATCCGCC
GAGTACGAGAACGTCGTCGAATGCCTCGCTGAAACCGGCGCGGAAATTCTCGTCGTTCCCAATGGGTCGCCCTACGCAAA
GGGCAAGAGCGATATCAGGCTGTCGATCTCGGTCGCGCGCGTGACCGAGAGCGATCTGCCGCTGGTCTATCTCAATCAGG
TCGGCGGCCAGGATGAACTGGTGTTCGACGGCGCTTCTTTCGTGCTGAACGCCGATCGCTCGCTGGCGGCGCAGCTTCCC
TTTTTCGAGGAAAGCGTCTTCACGCTGCAATGGACCAGGTCTGCCGAGGGGTGGCGCTGCGCCGGACCGGTCGCGCCGCA
GATCGACGGCGACAAGGCCGACTATGCCGCCTGCGTGCTGGGGTTGCGCGACTATGTCCGGAAGAACGGGTTTCCCGGCG
TTCTGCTCGGCGTGTCCGGCGGAATCGACTCGGCCTTGTGCGCGGCCATCGCCGTCGACGCTCTCGGGGCCGAGCAGGTT
CGCGGCGTCATGCTGCCGTTCCGTTTTACGGCGCAGGTCTCGCTCGACGATGCGGCAAGGCTCGCGACGGCGCTCGGCAT
TCGCTACGACGTGCTGCCGATCGCGCAGGCGGTGAATGGCTTCGAGGAGATTCTCTCCGGCGTGTTCGCGGGCGCGCCTC
GCGATATTACCGAGGAGAACCTGCAATCCCGCGCTCGCGGCACGCTTCTGATGGCGATGTCCAACAAGCTCGGTGCGATG
GTGGTGACCACCGGCAACAAATCGGAAATGTCGGTGGGGTACGCCACGCTGTACGGCGACATGAACGGCGGCTTCAATCC
GATCAAGGATATCTACAAGACCGAGGTGCTCCGGATGGCTAGCCTGCGCAACGCGTGGAAACCGGACGGCGCGCTTGGTC
CATCGGGCGAGGTGATCCCGCCCGGCGCCATCTCGCGGCCGCCGACGGCCGAGTTGCGCGAAGGCCAGCTGGATCAGGAT
TCGCTGCCGCCCTACGATCTGCTCGACGCGATTCTCGAGCGGTTGGTCGAGCGGGAGGAACCGCTCGCGGCGATCGTCGC
GGAAGGGTTCGACCACGACGTGGTCGTGCGCGTCGATCGCCTGCTGAATGCCGCCGAGTACAAGCGCCGGCAGGCGGCGC
CGGGCGTCAAGGTCACGCGCCGGAATTTCGGCCGCGACCGTCGCTATCCGATCACCAACCGGTTTCGCGATTCCGGCGAA
TCATTGCCGGCGCCGGATGAGACCCTCGTCTCCCGCGGCAGCCGGAGTTCCGCCGAAGCCTTTCAGGAATGA

Upstream 100 bases:

>100_bases
GACGGGCATGTTTTCTTGATCCGCCGCTGTCCATGCCGCTCGAACCCGCTATAACTCCGCCATATTGCAGCAAGGCGAGG
CCGGCGGGTAACAAGCATCC

Downstream 100 bases:

>100_bases
TGCGAGGAATGACCGGCGAATCGGAGGCCTTCCGCTTCTGATGTAAACACAACCGGAAAACTGCTGCTCGCCCTACTGCG
CGGGTATGAACGTGGGTCCC

Product: NAD synthetase

Products: NA

Alternate protein names: NAD(+) synthase [glutamine-hydrolyzing] [H]

Number of amino acids: Translated: 583; Mature: 582

Protein sequence:

>583_residues
MTETTFKITLGQLNPTVGDIVGNAAKARAARAQAKADGAALVALPELFIAGYPPEDLVLRPAFQAACREAIEALARETSD
GGPAMLIGTPWVDDGKLYNAYALLDEGRVAALRFKVNLPNYGVFDEKRVFARGPVGGPLKIRGVRIGVPICEDTWVEESA
EYENVVECLAETGAEILVVPNGSPYAKGKSDIRLSISVARVTESDLPLVYLNQVGGQDELVFDGASFVLNADRSLAAQLP
FFEESVFTLQWTRSAEGWRCAGPVAPQIDGDKADYAACVLGLRDYVRKNGFPGVLLGVSGGIDSALCAAIAVDALGAEQV
RGVMLPFRFTAQVSLDDAARLATALGIRYDVLPIAQAVNGFEEILSGVFAGAPRDITEENLQSRARGTLLMAMSNKLGAM
VVTTGNKSEMSVGYATLYGDMNGGFNPIKDIYKTEVLRMASLRNAWKPDGALGPSGEVIPPGAISRPPTAELREGQLDQD
SLPPYDLLDAILERLVEREEPLAAIVAEGFDHDVVVRVDRLLNAAEYKRRQAAPGVKVTRRNFGRDRRYPITNRFRDSGE
SLPAPDETLVSRGSRSSAEAFQE

Sequences:

>Translated_583_residues
MTETTFKITLGQLNPTVGDIVGNAAKARAARAQAKADGAALVALPELFIAGYPPEDLVLRPAFQAACREAIEALARETSD
GGPAMLIGTPWVDDGKLYNAYALLDEGRVAALRFKVNLPNYGVFDEKRVFARGPVGGPLKIRGVRIGVPICEDTWVEESA
EYENVVECLAETGAEILVVPNGSPYAKGKSDIRLSISVARVTESDLPLVYLNQVGGQDELVFDGASFVLNADRSLAAQLP
FFEESVFTLQWTRSAEGWRCAGPVAPQIDGDKADYAACVLGLRDYVRKNGFPGVLLGVSGGIDSALCAAIAVDALGAEQV
RGVMLPFRFTAQVSLDDAARLATALGIRYDVLPIAQAVNGFEEILSGVFAGAPRDITEENLQSRARGTLLMAMSNKLGAM
VVTTGNKSEMSVGYATLYGDMNGGFNPIKDIYKTEVLRMASLRNAWKPDGALGPSGEVIPPGAISRPPTAELREGQLDQD
SLPPYDLLDAILERLVEREEPLAAIVAEGFDHDVVVRVDRLLNAAEYKRRQAAPGVKVTRRNFGRDRRYPITNRFRDSGE
SLPAPDETLVSRGSRSSAEAFQE
>Mature_582_residues
TETTFKITLGQLNPTVGDIVGNAAKARAARAQAKADGAALVALPELFIAGYPPEDLVLRPAFQAACREAIEALARETSDG
GPAMLIGTPWVDDGKLYNAYALLDEGRVAALRFKVNLPNYGVFDEKRVFARGPVGGPLKIRGVRIGVPICEDTWVEESAE
YENVVECLAETGAEILVVPNGSPYAKGKSDIRLSISVARVTESDLPLVYLNQVGGQDELVFDGASFVLNADRSLAAQLPF
FEESVFTLQWTRSAEGWRCAGPVAPQIDGDKADYAACVLGLRDYVRKNGFPGVLLGVSGGIDSALCAAIAVDALGAEQVR
GVMLPFRFTAQVSLDDAARLATALGIRYDVLPIAQAVNGFEEILSGVFAGAPRDITEENLQSRARGTLLMAMSNKLGAMV
VTTGNKSEMSVGYATLYGDMNGGFNPIKDIYKTEVLRMASLRNAWKPDGALGPSGEVIPPGAISRPPTAELREGQLDQDS
LPPYDLLDAILERLVEREEPLAAIVAEGFDHDVVVRVDRLLNAAEYKRRQAAPGVKVTRRNFGRDRRYPITNRFRDSGES
LPAPDETLVSRGSRSSAEAFQE

Specific function: It is not known if this NAD synthase uses NH(3) or glutamine as the N donor [H]

COG id: COG0171

COG function: function code H; NAD synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014445
- InterPro:   IPR022310
- InterPro:   IPR003694
- InterPro:   IPR003010
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00795 CN_hydrolase; PF02540 NAD_synthase [H]

EC number: =6.3.5.1 [H]

Molecular weight: Translated: 62692; Mature: 62561

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTETTFKITLGQLNPTVGDIVGNAAKARAARAQAKADGAALVALPELFIAGYPPEDLVLR
CCCEEEEEEECCCCCHHHHHHCCHHHHHHHHHHHCCCCEEEEEEHHHHHCCCCCHHCEEC
PAFQAACREAIEALARETSDGGPAMLIGTPWVDDGKLYNAYALLDEGRVAALRFKVNLPN
HHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEEEECCCCEEEEEEEEECCC
YGVFDEKRVFARGPVGGPLKIRGVRIGVPICEDTWVEESAEYENVVECLAETGAEILVVP
CCCCCCCCEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEE
NGSPYAKGKSDIRLSISVARVTESDLPLVYLNQVGGQDELVFDGASFVLNADRSLAAQLP
CCCCCCCCCCCEEEEEEEEEECCCCCCEEEEECCCCCCEEEECCCEEEEECCCHHHHCCC
FFEESVFTLQWTRSAEGWRCAGPVAPQIDGDKADYAACVLGLRDYVRKNGFPGVLLGVSG
CCCCCEEEEEEECCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECC
GIDSALCAAIAVDALGAEQVRGVMLPFRFTAQVSLDDAARLATALGIRYDVLPIAQAVNG
CCHHHHHHHHHHHHCCHHHHCCEEECEEEEEEECHHHHHHHHHHHCCHHHHHHHHHHHHH
FEEILSGVFAGAPRDITEENLQSRARGTLLMAMSNKLGAMVVTTGNKSEMSVGYATLYGD
HHHHHHHHHCCCCCCCCHHHHHHHHCCEEEEEECCCCCEEEEECCCCCCCEEEEEEEEEC
MNGGFNPIKDIYKTEVLRMASLRNAWKPDGALGPSGEVIPPGAISRPPTAELREGQLDQD
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCC
SLPPYDLLDAILERLVEREEPLAAIVAEGFDHDVVVRVDRLLNAAEYKRRQAAPGVKVTR
CCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEHH
RNFGRDRRYPITNRFRDSGESLPAPDETLVSRGSRSSAEAFQE
HHCCCCCCCCCHHHHHCCCCCCCCCCHHHHHCCCCCHHHHHCC
>Mature Secondary Structure 
TETTFKITLGQLNPTVGDIVGNAAKARAARAQAKADGAALVALPELFIAGYPPEDLVLR
CCEEEEEEECCCCCHHHHHHCCHHHHHHHHHHHCCCCEEEEEEHHHHHCCCCCHHCEEC
PAFQAACREAIEALARETSDGGPAMLIGTPWVDDGKLYNAYALLDEGRVAALRFKVNLPN
HHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCEEEEEEEECCCCEEEEEEEEECCC
YGVFDEKRVFARGPVGGPLKIRGVRIGVPICEDTWVEESAEYENVVECLAETGAEILVVP
CCCCCCCCEEECCCCCCCEEEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHCCCEEEEEE
NGSPYAKGKSDIRLSISVARVTESDLPLVYLNQVGGQDELVFDGASFVLNADRSLAAQLP
CCCCCCCCCCCEEEEEEEEEECCCCCCEEEEECCCCCCEEEECCCEEEEECCCHHHHCCC
FFEESVFTLQWTRSAEGWRCAGPVAPQIDGDKADYAACVLGLRDYVRKNGFPGVLLGVSG
CCCCCEEEEEEECCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECC
GIDSALCAAIAVDALGAEQVRGVMLPFRFTAQVSLDDAARLATALGIRYDVLPIAQAVNG
CCHHHHHHHHHHHHCCHHHHCCEEECEEEEEEECHHHHHHHHHHHCCHHHHHHHHHHHHH
FEEILSGVFAGAPRDITEENLQSRARGTLLMAMSNKLGAMVVTTGNKSEMSVGYATLYGD
HHHHHHHHHCCCCCCCCHHHHHHHHCCEEEEEECCCCCEEEEECCCCCCCEEEEEEEEEC
MNGGFNPIKDIYKTEVLRMASLRNAWKPDGALGPSGEVIPPGAISRPPTAELREGQLDQD
CCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCC
SLPPYDLLDAILERLVEREEPLAAIVAEGFDHDVVVRVDRLLNAAEYKRRQAAPGVKVTR
CCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEHH
RNFGRDRRYPITNRFRDSGESLPAPDETLVSRGSRSSAEAFQE
HHCCCCCCCCCHHHHHCCCCCCCCCCHHHHHCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8431308; 8195100 [H]