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The map label for this gene is fliM [H]

Identifier: 75675317

GI number: 75675317

Start: 1233586

End: 1234791

Strand: Direct

Name: fliM [H]

Synonym: Nwi_1124

Alternate gene names: 75675317

Gene position: 1233586-1234791 (Clockwise)

Preceding gene: 75675316

Following gene: 75675318

Centisome position: 36.26

GC content: 61.94

Gene sequence:

>1206_bases
ATGGCGAACGACAGCGATCAGATCGATCAGGATGCAATCGCGGCCCAGTGGGAAGCCTCGCTCGACTCCGGGGATCCGGA
CGAAGCCGCGAAGTCCGCTGCCGCCAATGAACTCACGGGGGCGATGGCGGAGCAATGGGCCGCCATGGTCGACGACGGCG
GCCGTGATTTCGGCAACGGCAAGAACAGCGGCGAGCGGGTTCTGTCACAGGAGGAGATCGACAGCCTGCTCGGCTTCAGC
GTCGGCGAGATCAATCTCGACGACCATTCCGGTATCCGGGCGATCATCGACTCCGCGATGGTGTCTTATGAACGCCTGCC
GATGTTGGAGATCGTGTTCGACCGCCTGGTGCGGCTCATGACGACCTCCTTGCGCAATTTCACTTCCGACAACGTCGAGG
TCTCCCTTGACCGCATCACCTCGGTCCGCTTCGGCGACTATTTGAACTCGATACCCCTGCCGGCGGTGCTCAGCGTGTTC
AAGGCCGAGGAGTGGGAGAATTTCGGACTGGCGATGGTGGATTCCAGTCTGATCTACTCCATGATCGACGTCCTGCTGGG
CGGTCGTCGCGGTCAGTCATCGCTCCGCATCGAAGGCAGGCCCTATACGACGATCGAAACCAATCTGGTGAAGCGGCTGG
TGGAGGTCGTGCTGGCCGACGCCGAGCAGGCGTTCCGTCCGCTTTCGCCGGTGTCGTTCTCGATCGACCGCCTTGAAACC
AATCCGCGGTTCGCGGCGATCACGCGCCCGGCCAATGCCGCTATTCTGGTCCGGCTGCGGATCGATATGGAAGATCGCGG
CGGCACCATCGAACTGCTGCTGCCCTATGCCACCATTGAGCCGATCCGCAACGTCCTGTTGCAGATGTTCATGGGTGAAA
AGTTCGGCCGGGATCCGATCTGGGAAAGCCACCTCGCCACCGAGATCGCGCAGGCGCAGATCGCCGTCGACGCCGTTTTG
TACGAGGCCGAAATTCCGCTCAGGCAACTCATGACCCTGAAGGTCGGCGATACGCTCCCGCTTGAAATGCGTCCCGATGC
ATCGGTGGCGGTCCGCTGCGGCAGCGTTACGCTCACCGAGGGCCGCATGGGACGGGTCGGCGACCGCGTCGCCATTCGCG
TATCGCGGCATTTGCGCAAACCGAGCACGACATTCGCCATGTTTGAAAGAGCGGACGAGCAGACCAAGATGATGGAGGCC
CAATGA

Upstream 100 bases:

>100_bases
AAGTGGAGGCCGGTTTTCGGAGAAGACATGCTCGATCAAAAGTTAAGGCTGGAGCCTGATTCAACGCAGTTGAATCACAC
TCTTGCGCCGGAATGGGATC

Downstream 100 bases:

>100_bases
ACCACTCTTTCGGACTAGTGATCGAGAGTCTTGTCGCGGTTCTTCTCGTACTGACCATCGGTTACTGCATGCTGCTGAAC
AAGCGGCTGAAGCGGCTGAG

Product: flagellar motor switch protein FliM

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 401; Mature: 400

Protein sequence:

>401_residues
MANDSDQIDQDAIAAQWEASLDSGDPDEAAKSAAANELTGAMAEQWAAMVDDGGRDFGNGKNSGERVLSQEEIDSLLGFS
VGEINLDDHSGIRAIIDSAMVSYERLPMLEIVFDRLVRLMTTSLRNFTSDNVEVSLDRITSVRFGDYLNSIPLPAVLSVF
KAEEWENFGLAMVDSSLIYSMIDVLLGGRRGQSSLRIEGRPYTTIETNLVKRLVEVVLADAEQAFRPLSPVSFSIDRLET
NPRFAAITRPANAAILVRLRIDMEDRGGTIELLLPYATIEPIRNVLLQMFMGEKFGRDPIWESHLATEIAQAQIAVDAVL
YEAEIPLRQLMTLKVGDTLPLEMRPDASVAVRCGSVTLTEGRMGRVGDRVAIRVSRHLRKPSTTFAMFERADEQTKMMEA
Q

Sequences:

>Translated_401_residues
MANDSDQIDQDAIAAQWEASLDSGDPDEAAKSAAANELTGAMAEQWAAMVDDGGRDFGNGKNSGERVLSQEEIDSLLGFS
VGEINLDDHSGIRAIIDSAMVSYERLPMLEIVFDRLVRLMTTSLRNFTSDNVEVSLDRITSVRFGDYLNSIPLPAVLSVF
KAEEWENFGLAMVDSSLIYSMIDVLLGGRRGQSSLRIEGRPYTTIETNLVKRLVEVVLADAEQAFRPLSPVSFSIDRLET
NPRFAAITRPANAAILVRLRIDMEDRGGTIELLLPYATIEPIRNVLLQMFMGEKFGRDPIWESHLATEIAQAQIAVDAVL
YEAEIPLRQLMTLKVGDTLPLEMRPDASVAVRCGSVTLTEGRMGRVGDRVAIRVSRHLRKPSTTFAMFERADEQTKMMEA
Q
>Mature_400_residues
ANDSDQIDQDAIAAQWEASLDSGDPDEAAKSAAANELTGAMAEQWAAMVDDGGRDFGNGKNSGERVLSQEEIDSLLGFSV
GEINLDDHSGIRAIIDSAMVSYERLPMLEIVFDRLVRLMTTSLRNFTSDNVEVSLDRITSVRFGDYLNSIPLPAVLSVFK
AEEWENFGLAMVDSSLIYSMIDVLLGGRRGQSSLRIEGRPYTTIETNLVKRLVEVVLADAEQAFRPLSPVSFSIDRLETN
PRFAAITRPANAAILVRLRIDMEDRGGTIELLLPYATIEPIRNVLLQMFMGEKFGRDPIWESHLATEIAQAQIAVDAVLY
EAEIPLRQLMTLKVGDTLPLEMRPDASVAVRCGSVTLTEGRMGRVGDRVAIRVSRHLRKPSTTFAMFERADEQTKMMEAQ

Specific function: FliM is one of three proteins (FliG, FliN, FliM) that form the rotor-mounted switch complex (C ring), located at the base of the basal body. This complex interacts with the CheY and CheZ chemotaxis proteins, in addition to contacting components of the mot

COG id: COG1868

COG function: function code N; Flagellar motor switch protein

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein. Bacterial flagellum basal body [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FliM family [H]

Homologues:

Organism=Escherichia coli, GI1788255, Length=329, Percent_Identity=25.531914893617, Blast_Score=115, Evalue=4e-27,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001689
- InterPro:   IPR001543 [H]

Pfam domain/function: PF02154 FliM; PF01052 SpoA [H]

EC number: NA

Molecular weight: Translated: 44375; Mature: 44244

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANDSDQIDQDAIAAQWEASLDSGDPDEAAKSAAANELTGAMAEQWAAMVDDGGRDFGNG
CCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
KNSGERVLSQEEIDSLLGFSVGEINLDDHSGIRAIIDSAMVSYERLPMLEIVFDRLVRLM
CCHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
TTSLRNFTSDNVEVSLDRITSVRFGDYLNSIPLPAVLSVFKAEEWENFGLAMVDSSLIYS
HHHHHHCCCCCCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
MIDVLLGGRRGQSSLRIEGRPYTTIETNLVKRLVEVVLADAEQAFRPLSPVSFSIDRLET
HHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHCCC
NPRFAAITRPANAAILVRLRIDMEDRGGTIELLLPYATIEPIRNVLLQMFMGEKFGRDPI
CCCEEEEECCCCCEEEEEEEEEECCCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCC
WESHLATEIAQAQIAVDAVLYEAEIPLRQLMTLKVGDTLPLEMRPDASVAVRCGSVTLTE
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEECCCCCCCEECCCCCEEEEECCEEEEC
GRMGRVGDRVAIRVSRHLRKPSTTFAMFERADEQTKMMEAQ
CCCCCCCCHHHHEEHHHCCCCCHHHHHHHHCCHHHHHHCCC
>Mature Secondary Structure 
ANDSDQIDQDAIAAQWEASLDSGDPDEAAKSAAANELTGAMAEQWAAMVDDGGRDFGNG
CCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
KNSGERVLSQEEIDSLLGFSVGEINLDDHSGIRAIIDSAMVSYERLPMLEIVFDRLVRLM
CCHHHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
TTSLRNFTSDNVEVSLDRITSVRFGDYLNSIPLPAVLSVFKAEEWENFGLAMVDSSLIYS
HHHHHHCCCCCCEEEHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHH
MIDVLLGGRRGQSSLRIEGRPYTTIETNLVKRLVEVVLADAEQAFRPLSPVSFSIDRLET
HHHHHHCCCCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEHHHCCC
NPRFAAITRPANAAILVRLRIDMEDRGGTIELLLPYATIEPIRNVLLQMFMGEKFGRDPI
CCCEEEEECCCCCEEEEEEEEEECCCCCEEEEEECHHHHHHHHHHHHHHHHHHHCCCCCC
WESHLATEIAQAQIAVDAVLYEAEIPLRQLMTLKVGDTLPLEMRPDASVAVRCGSVTLTE
HHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHEEECCCCCCCEECCCCCEEEEECCEEEEC
GRMGRVGDRVAIRVSRHLRKPSTTFAMFERADEQTKMMEAQ
CCCCCCCCHHHHEEHHHCCCCCHHHHHHHHCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1315735 [H]