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The map label for this gene is rfaC [H]

Identifier: 75675259

GI number: 75675259

Start: 1166681

End: 1167625

Strand: Direct

Name: rfaC [H]

Synonym: Nwi_1066

Alternate gene names: 75675259

Gene position: 1166681-1167625 (Clockwise)

Preceding gene: 75675255

Following gene: 75675263

Centisome position: 34.29

GC content: 66.67

Gene sequence:

>945_bases
ATGACCGACGTCCTCATCATCAAGACATCCTCGCTCGGCGACGTCGTGCATCAGATGCCGGCGATCGTCGATGCGGCGCG
CGCATGTCCGCGGTTGCGGCTGACGTGGCTGGTCGAAGAGGCGTTCGCGCCGCTGGTGCGGCTGCATCCGTCGGTCGCTG
ATGTCATTCCTGTCGCGACGCGGCGCTGGCGTTCGCAACTCGGAGCCATCGCCACGTGGCGCGAGATCGGCGCGTTCCGG
GCGCGGCTGCGCCGGCAGGCGTTCGAAAAGGTGATCGATACCCAAGGTCTTATCCGCTCCGCGATCATGGCCCGCGTCTC
ACGCGGCGCGCGCCACGGCTACGATGCGCGCAGCATTCGCGAACCGTTGGCCGCGCGTTTCTACGATGTCCGGCACACGG
TTGGTCGCGACCTTCACGCCGTGACGCGCAACCGGCTGCTGACAGGTCTCTCGCTCGGGTACGATCCAACCGGGGATGTC
GATTATGGTCTCCGTTCTCCGGCGCGAACGGAGGGTCAGCGCTATGCCGTATTGCTTCACGGCACCTCCCGGCTCTCGAA
AACGTGGCGCGCGGAGGATTGGATCGAAACCGGCCGCTGGCTACAGGCGAACGGATTGCAGACCGTGCTGCCCTGGGGCA
CCGAGGCCGAGCGCCTGTTATCCGAGCGGCTGTCAGGAGAAATCGCGGGCAGTCGCGTCCAGCCGCGCCAGAGCCTTGAT
CTGACCGCGCGCATGATCGGCAATGCGGCGTTGGTGATCGGCGTCGACACCGGGTTGATGCATCTCGCCGCGGCCTATCG
GGTGCCGCTCATCGGCATCTATGTCAGTACCGATCCCGGCCTGACGGGGCCGATGGGCGCCGGACGCATGGCGGTTCTTG
GCGGCAAGAGCGGTCCGCCTCCCGCGGGGCAGGTGATCGATGCCGCGGAGCGATTGCTTGCTTAG

Upstream 100 bases:

>100_bases
ACCGTGATCGGCCGGATTTCGTCTGTCTGACCTCGGGATTCGACATTATGGTTGCCAAACTGTAACGGGAACCGGTTCTC
CCCTGGCCCTGCGGCAGCCG

Downstream 100 bases:

>100_bases
ATACGTGGTGTGATCACATCGGATATCTCCGCAAGCACCCGCGCGACGAATGCGTCGAGGTCGCCGCCGGGAAACAGGAA
TCCCGGAATTCCCGCGCCGG

Product: lipopolysaccharide heptosyltransferase I

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 313

Protein sequence:

>314_residues
MTDVLIIKTSSLGDVVHQMPAIVDAARACPRLRLTWLVEEAFAPLVRLHPSVADVIPVATRRWRSQLGAIATWREIGAFR
ARLRRQAFEKVIDTQGLIRSAIMARVSRGARHGYDARSIREPLAARFYDVRHTVGRDLHAVTRNRLLTGLSLGYDPTGDV
DYGLRSPARTEGQRYAVLLHGTSRLSKTWRAEDWIETGRWLQANGLQTVLPWGTEAERLLSERLSGEIAGSRVQPRQSLD
LTARMIGNAALVIGVDTGLMHLAAAYRVPLIGIYVSTDPGLTGPMGAGRMAVLGGKSGPPPAGQVIDAAERLLA

Sequences:

>Translated_314_residues
MTDVLIIKTSSLGDVVHQMPAIVDAARACPRLRLTWLVEEAFAPLVRLHPSVADVIPVATRRWRSQLGAIATWREIGAFR
ARLRRQAFEKVIDTQGLIRSAIMARVSRGARHGYDARSIREPLAARFYDVRHTVGRDLHAVTRNRLLTGLSLGYDPTGDV
DYGLRSPARTEGQRYAVLLHGTSRLSKTWRAEDWIETGRWLQANGLQTVLPWGTEAERLLSERLSGEIAGSRVQPRQSLD
LTARMIGNAALVIGVDTGLMHLAAAYRVPLIGIYVSTDPGLTGPMGAGRMAVLGGKSGPPPAGQVIDAAERLLA
>Mature_313_residues
TDVLIIKTSSLGDVVHQMPAIVDAARACPRLRLTWLVEEAFAPLVRLHPSVADVIPVATRRWRSQLGAIATWREIGAFRA
RLRRQAFEKVIDTQGLIRSAIMARVSRGARHGYDARSIREPLAARFYDVRHTVGRDLHAVTRNRLLTGLSLGYDPTGDVD
YGLRSPARTEGQRYAVLLHGTSRLSKTWRAEDWIETGRWLQANGLQTVLPWGTEAERLLSERLSGEIAGSRVQPRQSLDL
TARMIGNAALVIGVDTGLMHLAAAYRVPLIGIYVSTDPGLTGPMGAGRMAVLGGKSGPPPAGQVIDAAERLLA

Specific function: Heptose transfer to the lipopolysaccharide core. It transfers the innnermost heptose to [4'-P](3-deoxy-D-manno- octulosonic acid)2-IVA [H]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790051, Length=293, Percent_Identity=42.320819112628, Blast_Score=222, Evalue=3e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011908 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 34315; Mature: 34184

Theoretical pI: Translated: 11.03; Mature: 11.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDVLIIKTSSLGDVVHQMPAIVDAARACPRLRLTWLVEEAFAPLVRLHPSVADVIPVAT
CCCEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
RRWRSQLGAIATWREIGAFRARLRRQAFEKVIDTQGLIRSAIMARVSRGARHGYDARSIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
EPLAARFYDVRHTVGRDLHAVTRNRLLTGLSLGYDPTGDVDYGLRSPARTEGQRYAVLLH
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEE
GTSRLSKTWRAEDWIETGRWLQANGLQTVLPWGTEAERLLSERLSGEIAGSRVQPRQSLD
CCHHHHHHHHHHHHHHCCCEEECCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHHH
LTARMIGNAALVIGVDTGLMHLAAAYRVPLIGIYVSTDPGLTGPMGAGRMAVLGGKSGPP
HHHHHHCCEEEEEEECHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCEEEECCCCCCC
PAGQVIDAAERLLA
CCHHHHHHHHHHCC
>Mature Secondary Structure 
TDVLIIKTSSLGDVVHQMPAIVDAARACPRLRLTWLVEEAFAPLVRLHPSVADVIPVAT
CCEEEEECCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
RRWRSQLGAIATWREIGAFRARLRRQAFEKVIDTQGLIRSAIMARVSRGARHGYDARSIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
EPLAARFYDVRHTVGRDLHAVTRNRLLTGLSLGYDPTGDVDYGLRSPARTEGQRYAVLLH
HHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEE
GTSRLSKTWRAEDWIETGRWLQANGLQTVLPWGTEAERLLSERLSGEIAGSRVQPRQSLD
CCHHHHHHHHHHHHHHCCCEEECCCCEEECCCCCHHHHHHHHHHCCCCCCCCCCCHHHHH
LTARMIGNAALVIGVDTGLMHLAAAYRVPLIGIYVSTDPGLTGPMGAGRMAVLGGKSGPP
HHHHHHCCEEEEEEECHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCEEEECCCCCCC
PAGQVIDAAERLLA
CCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8478319; 8041620; 9278503; 1624462 [H]