Click here to switch to the map view.

The map label for this gene is ligA

Identifier: 75675255

GI number: 75675255

Start: 1160504

End: 1162654

Strand: Direct

Name: ligA

Synonym: Nwi_1062

Alternate gene names: 75675255

Gene position: 1160504-1162654 (Clockwise)

Preceding gene: 75675254

Following gene: 75675259

Centisome position: 34.11

GC content: 63.27

Gene sequence:

>2151_bases
GTGGCGGCCAAGTCAAAAACGCCTCCGCCCGTCGATTCCCTCACCAGGTCGCAGGCCAGGGTCGAGCACAAGAGGCTGGC
GCTTGAGATCGAGACGCATAACGAGCGCTACTACCAGCAGGACGCGCCAACGGTCTCCGACGCTACCTACGACGCGTTGC
AGCGGCGGCTCGAAGCGATCGAGGCCCGCTTTCCGGACCTCGTCACCGCAAGTTCGCCCACGCAGACGGTCGGCGCCGCG
CCCGCGCGCGGCTTCGCGAAAGTGCAGCATGCGGTGCCGATGCTGTCGCTCGGCAACGCCTTCAGCGACGCTGAGGTTGC
CGAGTTCGTCGAGCGCATCCGGCGTTTTCTGAAACTTGGTGCGGATGACATGCCCGCCATCGTTGCCGAGCCGAAGATCG
ATGGATTGTCGCTGTCGTTGCGTTACGAGAACGGCGACCTGGTGCGCGCGGCGACGCGCGGCGACGGCTTCACCGGCGAG
GACGTGACCGCGAATGTCCGCACCATCGCGGACGTGCCGCACAAACTGAACGGCCACAACATCCCCACCGCCTGCGAGCT
GCGCGGCGAGGTCTATATGCTGAAAGAGGACTTCCTTGCTCTGAATAAAAAGCAGCAAGAGGCTGGCGATACCGTGTTCG
CCAATCCGCGCAATTCGGCGGCCGGTTCTTTGCGCCAGAAGGATGTATCGATCACCGCCTCGCGTCCGTTGAAGTTCTTC
GCCTATGCCTGGGGCGAGATGACTGATCGGCCCGCCGAAACCCAGAACGACATGCTGGAGTGGCTCAGGGACGTTGGCTT
TATCGTCAATCCGCTGATCCAGCTATGTCACAGCGTCGATGAGGTGCTGGCGTTTTATCGCCGCATCGGTGAGCAACGCG
CCACGCTCGGCTACGACATCGACGGCGTGGTCTACAAGGTGGACCGGCTCGACTGGCAGGAACGCCTCGGCTTCGTGTCG
CGAAGCCCGCGCTGGGCGATCGCACACAAGTTCGCCGCCGAACAGGCGATGACGATCCTCAGGGGCATCGACATCCAGGT
CGGCCGGACCGGCGCGATGACGCCGGTGGCGCGGCTCGAACCCGTCACCGTCGGTGGCGTGGTGGTGCAGAATGCCACGC
TGCACAATGAGGATTACATCAGGGGCCTCGGCAACGATGGTCAGCCGATCCGCGACGGTATTGACCTTCGCATCGGCGAT
ACCGTGGTGGTGCAGCGCGCCGGCGATGTGATTCCGCAAGTGGTCAGCGTGATCATCGAAAAACGCCCGGCGAACGCTAA
GCGATATGAGTTTCCCGACAGGTGTCCGATCTGCGGCAGCCACGCCGTGCGCGAGGAGGGCGAGGCGGTGCGCCGTTGCA
CCGGCGCGCTGATCTGCCCGGCGCAGGCGGTGGAGCGGCTCAAGCATTTCGTCTCGCGCCTTGCTTTCGACATCGACGGC
CTCGGCGAAAAGCAGATACAGGAATTCTATGACGACGGCCTTGTCATGCACCCGGTCGATATCTTCACGTTGCAGGAGCG
CGACGCGCGTGCGGAGAACAAGCTGCGCGATCGCGACGGCTATGGCGATGTGTCGGTGCGCAACCTGTTCGCCGCGATCG
ACGCTCGCCGCAGGATCGAACTCAACCGGCTGATCTTCGCGCTCGGCATCCGCCACGTCGGCGAGGGCAATGCCAAGCTG
CTGGCGCGACACTATGGCAGTATCGAAAGTTTCCGCAGCGCCATGACGGAGGCCGCGGCGGCGCAGACGGAAGCAGGCAA
TGATTCCGAGGCCTATGCCGATCTTACCAACATCGGAGGCATCGGCGATATCGTTGCCGACGCGGTGGTCGAGTTCTTTG
CCGAGCCGCGCAACGTCAAGGCGCTGAACGATCTGCTTGAAGAAATCGAAGTGCTGCCGGTCGCGCAGGCCCGCAGCGAC
TCGGCCGTGGCGGGCAAGACCGTGGTGTTCACAGGCTCGCTGGAAAAATTCACGCGCGATGAAGCAAAAGCATCGGCCGA
GCGCGTGGGCGCGAAAACGTCCAGCTCGGTGTCGAAGAAGACCGATCTGGTCGTCGCAGGTCCCGGAGCCGGATCGAAGC
TCAAGGATGCCGAGAAGTTCGGCGTGAAAGTAATCTCCGAGGACGAGTGGCTGAAGCTGATCGAAGGATAG

Upstream 100 bases:

>100_bases
CCGCCGCGAGGAGATCGCGCGGATGCTTGCCGGAGCCGAGATCACCGCGGAGGCCCGCGCCGCCGCGGACCGGCTGTTGA
AAGCGGCGGGGTGAGTAGCT

Downstream 100 bases:

>100_bases
CCGATCTTCCGGAGTGCCGTGTCAAGGACGTTCAGGCCGCCGGCTGGCCGATCGTTATCGTCAGTTTGCCGACGCCGTCG
CAGCCGCATTCGACATGGTC

Product: NAD-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 716; Mature: 715

Protein sequence:

>716_residues
MAAKSKTPPPVDSLTRSQARVEHKRLALEIETHNERYYQQDAPTVSDATYDALQRRLEAIEARFPDLVTASSPTQTVGAA
PARGFAKVQHAVPMLSLGNAFSDAEVAEFVERIRRFLKLGADDMPAIVAEPKIDGLSLSLRYENGDLVRAATRGDGFTGE
DVTANVRTIADVPHKLNGHNIPTACELRGEVYMLKEDFLALNKKQQEAGDTVFANPRNSAAGSLRQKDVSITASRPLKFF
AYAWGEMTDRPAETQNDMLEWLRDVGFIVNPLIQLCHSVDEVLAFYRRIGEQRATLGYDIDGVVYKVDRLDWQERLGFVS
RSPRWAIAHKFAAEQAMTILRGIDIQVGRTGAMTPVARLEPVTVGGVVVQNATLHNEDYIRGLGNDGQPIRDGIDLRIGD
TVVVQRAGDVIPQVVSVIIEKRPANAKRYEFPDRCPICGSHAVREEGEAVRRCTGALICPAQAVERLKHFVSRLAFDIDG
LGEKQIQEFYDDGLVMHPVDIFTLQERDARAENKLRDRDGYGDVSVRNLFAAIDARRRIELNRLIFALGIRHVGEGNAKL
LARHYGSIESFRSAMTEAAAAQTEAGNDSEAYADLTNIGGIGDIVADAVVEFFAEPRNVKALNDLLEEIEVLPVAQARSD
SAVAGKTVVFTGSLEKFTRDEAKASAERVGAKTSSSVSKKTDLVVAGPGAGSKLKDAEKFGVKVISEDEWLKLIEG

Sequences:

>Translated_716_residues
MAAKSKTPPPVDSLTRSQARVEHKRLALEIETHNERYYQQDAPTVSDATYDALQRRLEAIEARFPDLVTASSPTQTVGAA
PARGFAKVQHAVPMLSLGNAFSDAEVAEFVERIRRFLKLGADDMPAIVAEPKIDGLSLSLRYENGDLVRAATRGDGFTGE
DVTANVRTIADVPHKLNGHNIPTACELRGEVYMLKEDFLALNKKQQEAGDTVFANPRNSAAGSLRQKDVSITASRPLKFF
AYAWGEMTDRPAETQNDMLEWLRDVGFIVNPLIQLCHSVDEVLAFYRRIGEQRATLGYDIDGVVYKVDRLDWQERLGFVS
RSPRWAIAHKFAAEQAMTILRGIDIQVGRTGAMTPVARLEPVTVGGVVVQNATLHNEDYIRGLGNDGQPIRDGIDLRIGD
TVVVQRAGDVIPQVVSVIIEKRPANAKRYEFPDRCPICGSHAVREEGEAVRRCTGALICPAQAVERLKHFVSRLAFDIDG
LGEKQIQEFYDDGLVMHPVDIFTLQERDARAENKLRDRDGYGDVSVRNLFAAIDARRRIELNRLIFALGIRHVGEGNAKL
LARHYGSIESFRSAMTEAAAAQTEAGNDSEAYADLTNIGGIGDIVADAVVEFFAEPRNVKALNDLLEEIEVLPVAQARSD
SAVAGKTVVFTGSLEKFTRDEAKASAERVGAKTSSSVSKKTDLVVAGPGAGSKLKDAEKFGVKVISEDEWLKLIEG
>Mature_715_residues
AAKSKTPPPVDSLTRSQARVEHKRLALEIETHNERYYQQDAPTVSDATYDALQRRLEAIEARFPDLVTASSPTQTVGAAP
ARGFAKVQHAVPMLSLGNAFSDAEVAEFVERIRRFLKLGADDMPAIVAEPKIDGLSLSLRYENGDLVRAATRGDGFTGED
VTANVRTIADVPHKLNGHNIPTACELRGEVYMLKEDFLALNKKQQEAGDTVFANPRNSAAGSLRQKDVSITASRPLKFFA
YAWGEMTDRPAETQNDMLEWLRDVGFIVNPLIQLCHSVDEVLAFYRRIGEQRATLGYDIDGVVYKVDRLDWQERLGFVSR
SPRWAIAHKFAAEQAMTILRGIDIQVGRTGAMTPVARLEPVTVGGVVVQNATLHNEDYIRGLGNDGQPIRDGIDLRIGDT
VVVQRAGDVIPQVVSVIIEKRPANAKRYEFPDRCPICGSHAVREEGEAVRRCTGALICPAQAVERLKHFVSRLAFDIDGL
GEKQIQEFYDDGLVMHPVDIFTLQERDARAENKLRDRDGYGDVSVRNLFAAIDARRRIELNRLIFALGIRHVGEGNAKLL
ARHYGSIESFRSAMTEAAAAQTEAGNDSEAYADLTNIGGIGDIVADAVVEFFAEPRNVKALNDLLEEIEVLPVAQARSDS
AVAGKTVVFTGSLEKFTRDEAKASAERVGAKTSSSVSKKTDLVVAGPGAGSKLKDAEKFGVKVISEDEWLKLIEG

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=688, Percent_Identity=47.3837209302326, Blast_Score=596, Evalue=1e-171,
Organism=Escherichia coli, GI87082305, Length=543, Percent_Identity=24.1252302025783, Blast_Score=109, Evalue=6e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_NITWN (Q3STR7)

Other databases:

- EMBL:   CP000115
- RefSeq:   YP_317676.1
- ProteinModelPortal:   Q3STR7
- STRING:   Q3STR7
- GeneID:   3675715
- GenomeReviews:   CP000115_GR
- KEGG:   nwi:Nwi_1062
- NMPDR:   fig|323098.3.peg.1764
- eggNOG:   COG0272
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- PhylomeDB:   Q3STR7
- ProtClustDB:   CLSK589823
- BioCyc:   NWIN323098:NWI_1062-MONOMER
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 78590; Mature: 78459

Theoretical pI: Translated: 5.62; Mature: 5.62

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 132-132 BINDING 130-130 BINDING 153-153 BINDING 190-190 BINDING 306-306 BINDING 330-330

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKSKTPPPVDSLTRSQARVEHKRLALEIETHNERYYQQDAPTVSDATYDALQRRLEAI
CCCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCHHHHCCCCCCCHHHHHHHHHHHHHH
EARFPDLVTASSPTQTVGAAPARGFAKVQHAVPMLSLGNAFSDAEVAEFVERIRRFLKLG
HHCCCCCEECCCCCCCCCCCCCHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHCC
ADDMPAIVAEPKIDGLSLSLRYENGDLVRAATRGDGFTGEDVTANVRTIADVPHKLNGHN
CCCCCCEEECCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHCCCCCCCCC
IPTACELRGEVYMLKEDFLALNKKQQEAGDTVFANPRNSAAGSLRQKDVSITASRPLKFF
CCCCEECCCCEEEEHHHHHHHCCHHHHCCCEEEECCCCCCCCCHHHCCEEEECCCCCEEH
AYAWGEMTDRPAETQNDMLEWLRDVGFIVNPLIQLCHSVDEVLAFYRRIGEQRATLGYDI
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DGVVYKVDRLDWQERLGFVSRSPRWAIAHKFAAEQAMTILRGIDIQVGRTGAMTPVARLE
CCEEEEEHHCCHHHHHCHHCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHCC
PVTVGGVVVQNATLHNEDYIRGLGNDGQPIRDGIDLRIGDTVVVQRAGDVIPQVVSVIIE
CEEECCEEEECCEECCCHHHHCCCCCCCCCCCCCCEEECCEEEEEHHHHHHHHHHHHHHH
KRPANAKRYEFPDRCPICGSHAVREEGEAVRRCTGALICPAQAVERLKHFVSRLAFDIDG
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEECHHHHHHHHHHHHHHHHHCCCC
LGEKQIQEFYDDGLVMHPVDIFTLQERDARAENKLRDRDGYGDVSVRNLFAAIDARRRIE
CCHHHHHHHHHCCCEECCEEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LNRLIFALGIRHVGEGNAKLLARHYGSIESFRSAMTEAAAAQTEAGNDSEAYADLTNIGG
HHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCC
IGDIVADAVVEFFAEPRNVKALNDLLEEIEVLPVAQARSDSAVAGKTVVFTGSLEKFTRD
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCHHHHHHH
EAKASAERVGAKTSSSVSKKTDLVVAGPGAGSKLKDAEKFGVKVISEDEWLKLIEG
HHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHCCEEECCCHHHHHCCC
>Mature Secondary Structure 
AAKSKTPPPVDSLTRSQARVEHKRLALEIETHNERYYQQDAPTVSDATYDALQRRLEAI
CCCCCCCCCHHHHHHHHHHHHHHHEEEEEECCCCHHHHCCCCCCCHHHHHHHHHHHHHH
EARFPDLVTASSPTQTVGAAPARGFAKVQHAVPMLSLGNAFSDAEVAEFVERIRRFLKLG
HHCCCCCEECCCCCCCCCCCCCHHHHHHHHHCCHHHCCCCCCHHHHHHHHHHHHHHHHCC
ADDMPAIVAEPKIDGLSLSLRYENGDLVRAATRGDGFTGEDVTANVRTIADVPHKLNGHN
CCCCCCEEECCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCHHHHHHCCCCCCCCC
IPTACELRGEVYMLKEDFLALNKKQQEAGDTVFANPRNSAAGSLRQKDVSITASRPLKFF
CCCCEECCCCEEEEHHHHHHHCCHHHHCCCEEEECCCCCCCCCHHHCCEEEECCCCCEEH
AYAWGEMTDRPAETQNDMLEWLRDVGFIVNPLIQLCHSVDEVLAFYRRIGEQRATLGYDI
HHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
DGVVYKVDRLDWQERLGFVSRSPRWAIAHKFAAEQAMTILRGIDIQVGRTGAMTPVARLE
CCEEEEEHHCCHHHHHCHHCCCCCHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCHHHCC
PVTVGGVVVQNATLHNEDYIRGLGNDGQPIRDGIDLRIGDTVVVQRAGDVIPQVVSVIIE
CEEECCEEEECCEECCCHHHHCCCCCCCCCCCCCCEEECCEEEEEHHHHHHHHHHHHHHH
KRPANAKRYEFPDRCPICGSHAVREEGEAVRRCTGALICPAQAVERLKHFVSRLAFDIDG
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCEECHHHHHHHHHHHHHHHHHCCCC
LGEKQIQEFYDDGLVMHPVDIFTLQERDARAENKLRDRDGYGDVSVRNLFAAIDARRRIE
CCHHHHHHHHHCCCEECCEEEEEECCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
LNRLIFALGIRHVGEGNAKLLARHYGSIESFRSAMTEAAAAQTEAGNDSEAYADLTNIGG
HHHHHHHHHHHHCCCCCHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCC
IGDIVADAVVEFFAEPRNVKALNDLLEEIEVLPVAQARSDSAVAGKTVVFTGSLEKFTRD
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEECCHHHHHHH
EAKASAERVGAKTSSSVSKKTDLVVAGPGAGSKLKDAEKFGVKVISEDEWLKLIEG
HHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHCCEEECCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA