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The map label for this gene is pntAA [H]

Identifier: 75675188

GI number: 75675188

Start: 1091776

End: 1092906

Strand: Direct

Name: pntAA [H]

Synonym: Nwi_0995

Alternate gene names: 75675188

Gene position: 1091776-1092906 (Clockwise)

Preceding gene: 75675187

Following gene: 75675189

Centisome position: 32.09

GC content: 63.84

Gene sequence:

>1131_bases
ATGAAGATTGCCATAGCGAAAGAAATCGATGAGTCGGAACCGCGGGTCGCCGCGTCACCCGATACAGTGAAGAAGTTCAA
GGCGCTCGGCGCGGATGTCGCAATCGAGCCGGGCGCCGGAACCAAATCGGGCGTGCCCGATTCGGACTTCACGGCCGCCG
GAGCGCTGGTAAGCCCGGAAGCCGTTAAAGACGCCGATATCATCATCAAGGTGAGGCGGCCCGAGGCATCCGAACTGGCG
CGCTACAAGCGTGGCGCGCTGGTGATCGCGATCATGGATCCTTACGGCAATGAGGCGGCGCTGAAGAAGATCGCGGATGC
CGGCGTATCGGCCTTCGCCATGGAGTTGATGCCGCGAATTACGCGCGCGCAGGTGATGGACGTGCTGTCGAGCCAGGCGA
ACCTGGCCGGCTACCGGGCCGTCATCGAGGCGGCCGAGGCGTTCGGCCGCGCCTTTCCAATGATGATGACGGCCGCCGGT
ACCGTGCCCGCTGCAAAGGTCTTCGTCATGGGGGTCGGCGTTGCCGGCCTTCAGGCGATCGCCACCGCGCGTCGCCTCGG
TGCCGCCGTCACCGCGACGGACGTGCGTCCCGCTACCAAGGAGCAGGTGGAAAGCCTCGGCGCCAAATTCCTTGCTGTCG
AGGACGAGGAGTTCAAGAACGCGCAGACCGCTGGCGGCTACGCCAAGGAAATGTCGAAAGAGTATCAGGCCAAGCAGGCG
GCGCTCACCGCTGAGCACATCAAGAAGCAGGACATCATCATCACCACGGCGCTGATTCCCGGACGGCCTGCGCCAAGGCT
CGTGACGGCGGAGATGGTGCGCTCGATGAAGCCGGGTTCGGTGCTGGTCGATCTCGCGGTCGAGCGAGGCGGTAACATCG
AGGGCGTCCAGGCGGGGAAGGTCGTCGATCTCAACGGCGTGAAGATCATCGGCTTTACCAATGTCGCCGGCCGCGTCGCG
GCCTCGGCGTCCAGCCTGTATGCCCGCAACCTGTTTTCCTTCATCGAAACGCTGGTCGACAAGACGACCAAGGCGCTGGC
GGTGAACTGGGACGACGAACTGGTCAAGGCTACCGCGCTGACCCGGGATGGCGCGGTCATTCATCCGAACTTCCAGCCGA
AAAGCGCATGA

Upstream 100 bases:

>100_bases
CCTGATTGTCAGTTCGTCGTTCCGGCCGTCCTGGATTTCCGCTCGCTAGCAATGTGGGTGGTGCTTTAGTCATGCGTGGC
CGTCGGCTGCGGGAGGCATC

Downstream 100 bases:

>100_bases
CGATCATGAGCCCAGCCGGTCAGCGGATTGTTCCGGACAGCACGACGCGCGCGTTGATTTGCGGATTTGCCGGTCACGCG
TCTCGCACGCCCGGAAACGA

Product: alanine dehydrogenase/PNT

Products: NA

Alternate protein names: Nicotinamide nucleotide transhydrogenase subunit alpha 1; Proton-translocating transhydrogenase component 1; Pyridine nucleotide transhydrogenase subunit alpha 1; dI [H]

Number of amino acids: Translated: 376; Mature: 376

Protein sequence:

>376_residues
MKIAIAKEIDESEPRVAASPDTVKKFKALGADVAIEPGAGTKSGVPDSDFTAAGALVSPEAVKDADIIIKVRRPEASELA
RYKRGALVIAIMDPYGNEAALKKIADAGVSAFAMELMPRITRAQVMDVLSSQANLAGYRAVIEAAEAFGRAFPMMMTAAG
TVPAAKVFVMGVGVAGLQAIATARRLGAAVTATDVRPATKEQVESLGAKFLAVEDEEFKNAQTAGGYAKEMSKEYQAKQA
ALTAEHIKKQDIIITTALIPGRPAPRLVTAEMVRSMKPGSVLVDLAVERGGNIEGVQAGKVVDLNGVKIIGFTNVAGRVA
ASASSLYARNLFSFIETLVDKTTKALAVNWDDELVKATALTRDGAVIHPNFQPKSA

Sequences:

>Translated_376_residues
MKIAIAKEIDESEPRVAASPDTVKKFKALGADVAIEPGAGTKSGVPDSDFTAAGALVSPEAVKDADIIIKVRRPEASELA
RYKRGALVIAIMDPYGNEAALKKIADAGVSAFAMELMPRITRAQVMDVLSSQANLAGYRAVIEAAEAFGRAFPMMMTAAG
TVPAAKVFVMGVGVAGLQAIATARRLGAAVTATDVRPATKEQVESLGAKFLAVEDEEFKNAQTAGGYAKEMSKEYQAKQA
ALTAEHIKKQDIIITTALIPGRPAPRLVTAEMVRSMKPGSVLVDLAVERGGNIEGVQAGKVVDLNGVKIIGFTNVAGRVA
ASASSLYARNLFSFIETLVDKTTKALAVNWDDELVKATALTRDGAVIHPNFQPKSA
>Mature_376_residues
MKIAIAKEIDESEPRVAASPDTVKKFKALGADVAIEPGAGTKSGVPDSDFTAAGALVSPEAVKDADIIIKVRRPEASELA
RYKRGALVIAIMDPYGNEAALKKIADAGVSAFAMELMPRITRAQVMDVLSSQANLAGYRAVIEAAEAFGRAFPMMMTAAG
TVPAAKVFVMGVGVAGLQAIATARRLGAAVTATDVRPATKEQVESLGAKFLAVEDEEFKNAQTAGGYAKEMSKEYQAKQA
ALTAEHIKKQDIIITTALIPGRPAPRLVTAEMVRSMKPGSVLVDLAVERGGNIEGVQAGKVVDLNGVKIIGFTNVAGRVA
ASASSLYARNLFSFIETLVDKTTKALAVNWDDELVKATALTRDGAVIHPNFQPKSA

Specific function: The transhydrogenation between NADH and NADP is coupled to respiration and ATP hydrolysis and functions as a proton pump across the membrane [H]

COG id: COG3288

COG function: function code C; NAD/NADP transhydrogenase alpha subunit

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AlaDH/PNT family [H]

Homologues:

Organism=Homo sapiens, GI122939155, Length=389, Percent_Identity=39.8457583547558, Blast_Score=252, Evalue=4e-67,
Organism=Homo sapiens, GI122939153, Length=389, Percent_Identity=39.8457583547558, Blast_Score=252, Evalue=4e-67,
Organism=Escherichia coli, GI1787887, Length=371, Percent_Identity=45.0134770889488, Blast_Score=301, Evalue=3e-83,
Organism=Caenorhabditis elegans, GI17550456, Length=366, Percent_Identity=48.0874316939891, Blast_Score=311, Evalue=3e-85,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007698
- InterPro:   IPR008142
- InterPro:   IPR008143
- InterPro:   IPR007886 [H]

Pfam domain/function: PF01262 AlaDh_PNT_C; PF05222 AlaDh_PNT_N [H]

EC number: =1.6.1.2 [H]

Molecular weight: Translated: 39480; Mature: 39480

Theoretical pI: Translated: 9.42; Mature: 9.42

Prosite motif: PS00837 ALADH_PNT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAIAKEIDESEPRVAASPDTVKKFKALGADVAIEPGAGTKSGVPDSDFTAAGALVSPE
CCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCEECCH
AVKDADIIIKVRRPEASELARYKRGALVIAIMDPYGNEAALKKIADAGVSAFAMELMPRI
HHCCCEEEEEECCCCHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHH
TRAQVMDVLSSQANLAGYRAVIEAAEAFGRAFPMMMTAAGTVPAAKVFVMGVGVAGLQAI
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHEECCHHHHHHH
ATARRLGAAVTATDVRPATKEQVESLGAKFLAVEDEEFKNAQTAGGYAKEMSKEYQAKQA
HHHHHHCCEEEECCCCCCHHHHHHHCCCEEEEECCHHHCCCCCCCHHHHHHHHHHHHHHH
ALTAEHIKKQDIIITTALIPGRPAPRLVTAEMVRSMKPGSVLVDLAVERGGNIEGVQAGK
HHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCC
VVDLNGVKIIGFTNVAGRVAASASSLYARNLFSFIETLVDKTTKALAVNWDDELVKATAL
EEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHE
TRDGAVIHPNFQPKSA
ECCCCEECCCCCCCCC
>Mature Secondary Structure
MKIAIAKEIDESEPRVAASPDTVKKFKALGADVAIEPGAGTKSGVPDSDFTAAGALVSPE
CCEEEECCCCCCCCCCCCCCHHHHHHHHCCCCEEECCCCCCCCCCCCCCCCCCCCEECCH
AVKDADIIIKVRRPEASELARYKRGALVIAIMDPYGNEAALKKIADAGVSAFAMELMPRI
HHCCCEEEEEECCCCHHHHHHHHCCEEEEEEECCCCCHHHHHHHHHCCHHHHHHHHHHHH
TRAQVMDVLSSQANLAGYRAVIEAAEAFGRAFPMMMTAAGTVPAAKVFVMGVGVAGLQAI
HHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHEECCHHHHHHH
ATARRLGAAVTATDVRPATKEQVESLGAKFLAVEDEEFKNAQTAGGYAKEMSKEYQAKQA
HHHHHHCCEEEECCCCCCHHHHHHHCCCEEEEECCHHHCCCCCCCHHHHHHHHHHHHHHH
ALTAEHIKKQDIIITTALIPGRPAPRLVTAEMVRSMKPGSVLVDLAVERGGNIEGVQAGK
HHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCC
VVDLNGVKIIGFTNVAGRVAASASSLYARNLFSFIETLVDKTTKALAVNWDDELVKATAL
EEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHE
TRDGAVIHPNFQPKSA
ECCCCEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8075801 [H]