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The map label for this gene is 75675052

Identifier: 75675052

GI number: 75675052

Start: 931699

End: 932121

Strand: Direct

Name: 75675052

Synonym: Nwi_0857

Alternate gene names: NA

Gene position: 931699-932121 (Clockwise)

Preceding gene: 75675051

Following gene: 75675053

Centisome position: 27.39

GC content: 64.3

Gene sequence:

>423_bases
TTGAGTTATCTGCTCGACACCAACGTCGTCTCCGAGCTGCGCAAGATCGGCGACGGAAAGGCCGATGCCAGGGTCGCCGC
ATGGGTCGACGCGGAGGAAGCCGAGAGCTTCTTCATCTCGGCCATCACCATCCTTGAACTGGAACGCGGCGTGCTCGCCA
TCCAGCGTCGCGACGCCGCGCAGGGCGCGCGCCTGCGCGCTTGGCTGGACAATCATGTCCGACCGGAATTTGCCGGGCGC
ATCCTGCCTGTCGACGACCAGATTGCGACGCGCTGCGCGCATCTGCATATTCCCGATCGGCGCAACGAGGTCGATGCGCT
GATTGCCGCGACAGCGCTTGTCCACGGCTTGACCGTGGCGACGCGCAACTTGCGGGATTTCGAGGGCACTGGCGTTGTCG
TGGTCAACCCTTGGGAGGGTTGA

Upstream 100 bases:

>100_bases
AGCGCCGCAGCTTGACGCAAGCGCTTGCCATGCCGGGCCTTTCGGACATCGATTTTGATCCGCCGCGTGTCGAAATCGCG
CCGCGCGGGGTCGATCTGTC

Downstream 100 bases:

>100_bases
CCCGGTTTCACACGTCGATGCGCTGCCGGCATGTGGGCTGGCCGCTGCCCCAGGACTTCTTCCTGCAAGCGGTCGCGGTG
GAGCGGGCCTTCTTCGACAC

Product: PilT protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 140; Mature: 139

Protein sequence:

>140_residues
MSYLLDTNVVSELRKIGDGKADARVAAWVDAEEAESFFISAITILELERGVLAIQRRDAAQGARLRAWLDNHVRPEFAGR
ILPVDDQIATRCAHLHIPDRRNEVDALIAATALVHGLTVATRNLRDFEGTGVVVVNPWEG

Sequences:

>Translated_140_residues
MSYLLDTNVVSELRKIGDGKADARVAAWVDAEEAESFFISAITILELERGVLAIQRRDAAQGARLRAWLDNHVRPEFAGR
ILPVDDQIATRCAHLHIPDRRNEVDALIAATALVHGLTVATRNLRDFEGTGVVVVNPWEG
>Mature_139_residues
SYLLDTNVVSELRKIGDGKADARVAAWVDAEEAESFFISAITILELERGVLAIQRRDAAQGARLRAWLDNHVRPEFAGRI
LPVDDQIATRCAHLHIPDRRNEVDALIAATALVHGLTVATRNLRDFEGTGVVVVNPWEG

Specific function: Involved in plasmid stability (Potential) [H]

COG id: COG1487

COG function: function code R; Predicted nucleic acid-binding protein, contains PIN domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002716 [H]

Pfam domain/function: PF01850 PIN [H]

EC number: NA

Molecular weight: Translated: 15437; Mature: 15305

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYLLDTNVVSELRKIGDGKADARVAAWVDAEEAESFFISAITILELERGVLAIQRRDAA
CCCEECHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCEEEEECCCCC
QGARLRAWLDNHVRPEFAGRILPVDDQIATRCAHLHIPDRRNEVDALIAATALVHGLTVA
CCCHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
TRNLRDFEGTGVVVVNPWEG
HCCCCCCCCCCEEEEECCCC
>Mature Secondary Structure 
SYLLDTNVVSELRKIGDGKADARVAAWVDAEEAESFFISAITILELERGVLAIQRRDAA
CCEECHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHHCCEEEEECCCCC
QGARLRAWLDNHVRPEFAGRILPVDDQIATRCAHLHIPDRRNEVDALIAATALVHGLTVA
CCCHHHHHHHCCCCHHHCCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHH
TRNLRDFEGTGVVVVNPWEG
HCCCCCCCCCCEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]