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The map label for this gene is narH [H]

Identifier: 75674972

GI number: 75674972

Start: 864442

End: 865983

Strand: Direct

Name: narH [H]

Synonym: Nwi_0776

Alternate gene names: 75674972

Gene position: 864442-865983 (Clockwise)

Preceding gene: 75674970

Following gene: 75674973

Centisome position: 25.41

GC content: 61.09

Gene sequence:

>1542_bases
ATGGACATCCGAGCTCAAGTTTCGATGGTCTTTCACCTGGACAAGTGCATCGGTTGCCACACCTGCAGCATCGCGTGCAA
GAACATCTGGACCGATCGCAAGGGCACCGAGTACATGTACTGGAACAACGTGGAGACCAAGCCGGGTACGGGTTACCCGA
CACGTTGGGAAGACCAGACCAAGTATCGCGGCGGCTGGGTGGTTGACGGTCAAAGGCAGAAGAGCCTTCGGCTTCGGCTG
CAGGGCAAGTGGGGAACGCTGACGAACATCTTCTACAACCCCTATCTGCCGACGCTCGACGACTATTTCGAGCCGTGGAC
CTACGATTACCAGAACCTGATCACTGCGCCTCTGGCTGACGAGCAGCCGACCGCGCGGGCGATTTCGATGGTGACCGGCA
AGTACATGGACACGATTGAGGCGGGTCCGAACTGGGACGACGACCTCGGCGGTTCGCAGGTTTACGCCAACAACGACCCG
AACTTCGACGGCGCCTCCGACGAGGAAATGCGCCAGATCAACGAGATCAACAGCACGGTGTTCTTCTATCTGCCGCGCAT
CTGCAACCATTGCCTCAATCCGGGATGCGTTGCGGCCTGCCCGCAGGGCGCGCTCTACAAGCGTGGTGAGGACGGCGTCG
TGCTGGTGAGCCAGGAACGCTGCCGGGCCTGGCGCATGTGTGTCTCGGGCTGCCCCTACAAGAAGACCTACTTCAACTGG
TCGACCGGCAAGGCCGAGAAGTGCATCCTGTGCTATCCGCGTCTTGAGAGCGGCCAGCCGCCGGCGTGCTTCCACTCCTG
CGTGGGACGCATCCGCTATATCGGCCTCGTGCTTTACGATGCGGACGCGATCGAGGAGACGGCGAAGTCGCCGCAGGATC
AGCTGGTGATGGCCCAGCGCAACATCATCAAGGATCCGTTCGATCCGGAGATCATCGCCGCCGCGCGAGCGAACGGGATT
CCGGACTCGAAGATCGAAGCGGCGCAGAAATCTCCGGTATTTCAGTTCGTGAAGAAGTGGGGCATCGCGCTGCCGTTGCA
TCCGGAGTTCCGCACGTTGCCGATGCTGTTCTATGTGCCGCCGCTGGGGCCGGTTCTGGCCAAGGTGGAGAACGGCGTAT
ACGACAACGTTGCCAACGAAGCGCGGCTTGGACCGTTGATGAGTTCGCTCGAGCGATCACGTATTCCGCTTCGTTACATG
GCGAGTCTCCTCGCGGGCGGCAATGAGGAGATTATCCGCGACGTCTACAAGAAGCTGGTTGCGGTGCGCGTCTACATGCG
TTCGCGGAAGGTCAAGGACATTCCGGACGAGGAGGTGCAGCGGGCGCTGTCGGAAGGCAAGACCACCGCGGCGGAAGTCG
AGGCGATCTGGCGTCTGACATCGATGCCGACCTTCGAGGAGCGCTTCGTGGTTCCGCCGATGGAGCGTGAGACGGCGGTC
GACGCCCTGTTCCCGCAGCTTGATCCGGTCTCTCATAACTATCCGATCCGCAAAGGCGAGGTCGGCGTCGGCTTCCACAC
CGATCCGGCAAGAGGACCATAG

Upstream 100 bases:

>100_bases
TGCGCCTGATCCCATCGATGAGAATGGTGGCGCATAAGTGCGCGACGACACTCGAGAATGAAGTCATAAGAACGAGCCAT
AGGAACGAAGGAGTAGACAC

Downstream 100 bases:

>100_bases
ACCCCTTGCTGTATGACAAGACGCGAGACAGTTTTCGGATCATGCATCCGGACCTGTCTCGCGCAGGGTAGTCTGGAAGT
AGACTGTCCTTATGAACTAT

Product: nitrate reductase, beta subunit

Products: NA

Alternate protein names: Nitrate reductase A subunit beta; Quinol-nitrate oxidoreductase subunit beta [H]

Number of amino acids: Translated: 513; Mature: 513

Protein sequence:

>513_residues
MDIRAQVSMVFHLDKCIGCHTCSIACKNIWTDRKGTEYMYWNNVETKPGTGYPTRWEDQTKYRGGWVVDGQRQKSLRLRL
QGKWGTLTNIFYNPYLPTLDDYFEPWTYDYQNLITAPLADEQPTARAISMVTGKYMDTIEAGPNWDDDLGGSQVYANNDP
NFDGASDEEMRQINEINSTVFFYLPRICNHCLNPGCVAACPQGALYKRGEDGVVLVSQERCRAWRMCVSGCPYKKTYFNW
STGKAEKCILCYPRLESGQPPACFHSCVGRIRYIGLVLYDADAIEETAKSPQDQLVMAQRNIIKDPFDPEIIAAARANGI
PDSKIEAAQKSPVFQFVKKWGIALPLHPEFRTLPMLFYVPPLGPVLAKVENGVYDNVANEARLGPLMSSLERSRIPLRYM
ASLLAGGNEEIIRDVYKKLVAVRVYMRSRKVKDIPDEEVQRALSEGKTTAAEVEAIWRLTSMPTFEERFVVPPMERETAV
DALFPQLDPVSHNYPIRKGEVGVGFHTDPARGP

Sequences:

>Translated_513_residues
MDIRAQVSMVFHLDKCIGCHTCSIACKNIWTDRKGTEYMYWNNVETKPGTGYPTRWEDQTKYRGGWVVDGQRQKSLRLRL
QGKWGTLTNIFYNPYLPTLDDYFEPWTYDYQNLITAPLADEQPTARAISMVTGKYMDTIEAGPNWDDDLGGSQVYANNDP
NFDGASDEEMRQINEINSTVFFYLPRICNHCLNPGCVAACPQGALYKRGEDGVVLVSQERCRAWRMCVSGCPYKKTYFNW
STGKAEKCILCYPRLESGQPPACFHSCVGRIRYIGLVLYDADAIEETAKSPQDQLVMAQRNIIKDPFDPEIIAAARANGI
PDSKIEAAQKSPVFQFVKKWGIALPLHPEFRTLPMLFYVPPLGPVLAKVENGVYDNVANEARLGPLMSSLERSRIPLRYM
ASLLAGGNEEIIRDVYKKLVAVRVYMRSRKVKDIPDEEVQRALSEGKTTAAEVEAIWRLTSMPTFEERFVVPPMERETAV
DALFPQLDPVSHNYPIRKGEVGVGFHTDPARGP
>Mature_513_residues
MDIRAQVSMVFHLDKCIGCHTCSIACKNIWTDRKGTEYMYWNNVETKPGTGYPTRWEDQTKYRGGWVVDGQRQKSLRLRL
QGKWGTLTNIFYNPYLPTLDDYFEPWTYDYQNLITAPLADEQPTARAISMVTGKYMDTIEAGPNWDDDLGGSQVYANNDP
NFDGASDEEMRQINEINSTVFFYLPRICNHCLNPGCVAACPQGALYKRGEDGVVLVSQERCRAWRMCVSGCPYKKTYFNW
STGKAEKCILCYPRLESGQPPACFHSCVGRIRYIGLVLYDADAIEETAKSPQDQLVMAQRNIIKDPFDPEIIAAARANGI
PDSKIEAAQKSPVFQFVKKWGIALPLHPEFRTLPMLFYVPPLGPVLAKVENGVYDNVANEARLGPLMSSLERSRIPLRYM
ASLLAGGNEEIIRDVYKKLVAVRVYMRSRKVKDIPDEEVQRALSEGKTTAAEVEAIWRLTSMPTFEERFVVPPMERETAV
DALFPQLDPVSHNYPIRKGEVGVGFHTDPARGP

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons ar

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787478, Length=490, Percent_Identity=49.5918367346939, Blast_Score=506, Evalue=1e-144,
Organism=Escherichia coli, GI1787740, Length=484, Percent_Identity=48.9669421487603, Blast_Score=506, Evalue=1e-144,
Organism=Escherichia coli, GI1787122, Length=86, Percent_Identity=43.0232558139535, Blast_Score=80, Evalue=4e-16,
Organism=Escherichia coli, GI1787872, Length=96, Percent_Identity=40.625, Blast_Score=79, Evalue=5e-16,
Organism=Escherichia coli, GI1787749, Length=87, Percent_Identity=35.632183908046, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1790326, Length=139, Percent_Identity=29.4964028776978, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI226510944, Length=92, Percent_Identity=36.9565217391304, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1789370, Length=93, Percent_Identity=35.4838709677419, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI2367345, Length=88, Percent_Identity=31.8181818181818, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 58032; Mature: 58032

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIRAQVSMVFHLDKCIGCHTCSIACKNIWTDRKGTEYMYWNNVETKPGTGYPTRWEDQT
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KYRGGWVVDGQRQKSLRLRLQGKWGTLTNIFYNPYLPTLDDYFEPWTYDYQNLITAPLAD
CCCCCEEECCCCCCCEEEEEECCCCCCHHEEECCCCCCHHHHCCCCCCCHHHHEECCCCC
EQPTARAISMVTGKYMDTIEAGPNWDDDLGGSQVYANNDPNFDGASDEEMRQINEINSTV
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHH
FFYLPRICNHCLNPGCVAACPQGALYKRGEDGVVLVSQERCRAWRMCVSGCPYKKTYFNW
HHHHHHHHHHHCCCCCEEECCCCCCEECCCCCEEEEEHHHHHHHHHHHCCCCCCCEEEEC
STGKAEKCILCYPRLESGQPPACFHSCVGRIRYIGLVLYDADAIEETAKSPQDQLVMAQR
CCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHEEEEECCHHHHHHHCCCHHHHHHHHH
NIIKDPFDPEIIAAARANGIPDSKIEAAQKSPVFQFVKKWGIALPLHPEFRTLPMLFYVP
HHCCCCCCCHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEC
PLGPVLAKVENGVYDNVANEARLGPLMSSLERSRIPLRYMASLLAGGNEEIIRDVYKKLV
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHH
AVRVYMRSRKVKDIPDEEVQRALSEGKTTAAEVEAIWRLTSMPTFEERFVVPPMERETAV
HHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHH
DALFPQLDPVSHNYPIRKGEVGVGFHTDPARGP
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MDIRAQVSMVFHLDKCIGCHTCSIACKNIWTDRKGTEYMYWNNVETKPGTGYPTRWEDQT
CCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KYRGGWVVDGQRQKSLRLRLQGKWGTLTNIFYNPYLPTLDDYFEPWTYDYQNLITAPLAD
CCCCCEEECCCCCCCEEEEEECCCCCCHHEEECCCCCCHHHHCCCCCCCHHHHEECCCCC
EQPTARAISMVTGKYMDTIEAGPNWDDDLGGSQVYANNDPNFDGASDEEMRQINEINSTV
CCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHHHH
FFYLPRICNHCLNPGCVAACPQGALYKRGEDGVVLVSQERCRAWRMCVSGCPYKKTYFNW
HHHHHHHHHHHCCCCCEEECCCCCCEECCCCCEEEEEHHHHHHHHHHHCCCCCCCEEEEC
STGKAEKCILCYPRLESGQPPACFHSCVGRIRYIGLVLYDADAIEETAKSPQDQLVMAQR
CCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHEEEEECCHHHHHHHCCCHHHHHHHHH
NIIKDPFDPEIIAAARANGIPDSKIEAAQKSPVFQFVKKWGIALPLHPEFRTLPMLFYVP
HHCCCCCCCHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCCEEEEEC
PLGPVLAKVENGVYDNVANEARLGPLMSSLERSRIPLRYMASLLAGGNEEIIRDVYKKLV
CCHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHH
AVRVYMRSRKVKDIPDEEVQRALSEGKTTAAEVEAIWRLTSMPTFEERFVVPPMERETAV
HHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCHHHCCCCCCCCHHHHH
DALFPQLDPVSHNYPIRKGEVGVGFHTDPARGP
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2674654; 8905232; 9278503; 2832376; 3053688; 1321049; 8383531; 8388253; 8664273; 9516445; 12910261 [H]