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The map label for this gene is nasD [H]

Identifier: 75674916

GI number: 75674916

Start: 804319

End: 806781

Strand: Direct

Name: nasD [H]

Synonym: Nwi_0719

Alternate gene names: 75674916

Gene position: 804319-806781 (Clockwise)

Preceding gene: 75674914

Following gene: 75674917

Centisome position: 23.64

GC content: 62.73

Gene sequence:

>2463_bases
ATGAACAAGAAAGAGAAGCTCGTCATCGTCGGCGCGGGCATGGCCTCCGGCCGCATGCTCGAGCATCTGTTCGAGGCTGA
CCCTGAACGTTTCGATGTTACGCTTTTCGGCGCGGAGCCGCGCGGAAATTACAATCGCATCATGCTGTCGCCGGTGCTGG
CGGGCGAGAAGTCGTTCGATCAGATCGTCACGCATGACGCGGCCTGGTACGACGCCCATCAGGTCGAATGCCGCTTCGGC
GAGACGGTGACGCGCATCGATCGCGCCGCGAAGGTGGTGCACTCGGCGGGAAGCGAAGCTCCTTACGACAAGCTTGTGAT
CGCGACCGGATCCGCTCCCTTCATGATCCCCGTCGCCGGGCGGGACTTGCCGGGCGTCATGGCGTTTCGGGACTATGACG
ATGTTCAGGCGATGGTCGCCGCCGCCGGGAAAAAAGACGCCAAGGCTGTCGTCATCGGCGGCGGCCTTCTCGGACTGGAG
GCCGCGGCGGCGCTCAGGCTGCATGGCATGGAGGTGGTGGTTCTGCATCTGATGGGCCACCTGATGGAGCGTCAGCTGGA
TGCGGCGGCGGGCTATCTCCTGCAGAAGGAGCTGGAGAGCCGCGGCATCAAGATTCATTGCAAGGCGCAGACCAGTGCGA
TCCTCGGACGCGGGCGCACCGAAGCGGTTCTGCTCGATGACGGCACCCTCTATCCCGCCGATATCGTGGTGATGGCGGTT
GGCATTCGTCCCGAAGCGCGGATCGCGACCGATGCGGGTCTCCATGTCGAGCGCGGCATCGTCGTGGACGACCGGATGCT
GACGTCCGATCCGGACATTCTCGCCCTCGGAGAATGCTGCGAGCACAACAACATCTGCTACGGCCTCGTCGCGCCGCTCT
ATGATATGGCCAAGGTCGCGGCGAAGACCTTGACAGGAGTTGAAGCGACGTTCCGCAGCGTCGACACAGCGACCCAGTTG
AAGGTGACCGGCGTGAGCCTTTACTCGGCGGGCGACTTCGCCGACGCGCCTGACCGCGAAGATATCGTGCTGCGGAATTC
CGGCGCGGGAATCTACAAGCGGCTCGTGCTGAAGGACAACCGCATTGTCGGCGTGGTCCTTTACGGCGATACCACGGACG
GCGCCTGGTACTTCGATATGCTGCGCAAGGGGACGGATGTCTCGGAGTTGCGCGAGACGCTGATCTTCGGTCAATCCCGT
CAGGGAAACGCGTCCCTCGACCCGATGGCGGCGGTCGCGGCCTTGCCGGATGATGCGGAGATCTGCGGCTGCAACGGCGT
TTGCAAGGGCGCGATCGTCTCGGCCATCATCGCCAGGGATCTGACATCGCTCGACGACGTACGTGCCCACACCAAGGCGT
CCGCCTCCTGTGGGTCCTGCACGGGTCTCGTCGAGCAGCTCATGACACTGACGCTCGGGGACAAGTACAATCCCGACGCC
GTCCAGCCGGTGTGTGGCTGCACCACGCTCGGACATGGCGATGTCCGGCGGCTGATCAAGGCGAAGCGTCTAAAGACCAT
TCCCGCCGTGATGCAGGAACTGGAATGGAAGACCTCATGCGGCTGCGCGAAGTGCCGGCCGGCGCTCAACTACTATCTCG
TCTGCGACTGGCCGGACGAATATGCCGACGACTACCAGTCGCGTTTCGTTAACGAGCGTGTTCATGCCAACATTCAAAAG
GACGGCACCTATTCGGTCGTGCCGCGCATGTGGGGCGGCGTCACCAGCGCGAAGGAATTGCGCGCCATCGCCGACGTGGT
CGATAAATTTCACATTCCCTCCGTGAAGGTCACGGGCGGCCAGCGTATCGACATGCTGGGCGTCAGGAAGGAAGACCTGC
CTGCGGTATGGGCCGATCTCGGCAGGGCCGGCTTCATCTCGGGTCATGCCTATGCCAAGGGCCTGCGCACGGTGAAGACC
TGCGTCGGGTCGGACTGGTGCCGCTTCGGCACGCAGGATTCGACCGGGCTCGGCATTCGTATCGAGAAATTCATGTGGGG
TGCGTGGACGCCGGCCAAGGTCAAGATGGCGGTCTCGGGTTGTCCGCGCAACTGCGCCGAGGCGACCTGCAAGGACGTCG
GCGTGATCTGCGTCGACTCCGGTTATGAGATCCATTTCGCCGGCGCGGCGGGCCTCGATATCAGGGGCACGGAAGTTCTG
GGACTTGTCAAAACGGAAGATGACGCGCTGGAACATATCGTGGCGCTCGTCCAGATGTACCGCGAGCAGGCCCACTATCT
TGAGCGCATCTACAAATGGGCGAAGCGCGTCGGCCTCGACGAGATCCGCCGCCAGATTTTGGACGATCCGCAGAAGCGCA
AGGCTTATTTCGATCGCTTCGCCTTCAGCCGCAAGTTCGCGCAGGTCGATCCTTGGTCTGAACGCGTGTCCGGCAAGGAC
AAGCACGAATTCCGGCCGATGGCGACCATCGGTTTTCCGGTGGCGGCGGAGGAAGCATTATGA

Upstream 100 bases:

>100_bases
CGGCGTTCTGATAGCGGCGTGCCGTATTTTCAGGTCTGGCACAGGTCTTGCGTAATCGTCCGTGACGGCTCTCGCTATCT
GAGGACGGAGCAAGATCGAG

Downstream 100 bases:

>100_bases
GCTGGATTGCGATTGGCGGGACGGAGGATATTCCGCTTCGCGGCGCGCGCTGCGTGGCGACGCCTCTAGGGCGGATTGCG
GTATTCCGCACTGTCGAGAA

Product: BFD-like (2Fe-2S)-binding region

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 820; Mature: 820

Protein sequence:

>820_residues
MNKKEKLVIVGAGMASGRMLEHLFEADPERFDVTLFGAEPRGNYNRIMLSPVLAGEKSFDQIVTHDAAWYDAHQVECRFG
ETVTRIDRAAKVVHSAGSEAPYDKLVIATGSAPFMIPVAGRDLPGVMAFRDYDDVQAMVAAAGKKDAKAVVIGGGLLGLE
AAAALRLHGMEVVVLHLMGHLMERQLDAAAGYLLQKELESRGIKIHCKAQTSAILGRGRTEAVLLDDGTLYPADIVVMAV
GIRPEARIATDAGLHVERGIVVDDRMLTSDPDILALGECCEHNNICYGLVAPLYDMAKVAAKTLTGVEATFRSVDTATQL
KVTGVSLYSAGDFADAPDREDIVLRNSGAGIYKRLVLKDNRIVGVVLYGDTTDGAWYFDMLRKGTDVSELRETLIFGQSR
QGNASLDPMAAVAALPDDAEICGCNGVCKGAIVSAIIARDLTSLDDVRAHTKASASCGSCTGLVEQLMTLTLGDKYNPDA
VQPVCGCTTLGHGDVRRLIKAKRLKTIPAVMQELEWKTSCGCAKCRPALNYYLVCDWPDEYADDYQSRFVNERVHANIQK
DGTYSVVPRMWGGVTSAKELRAIADVVDKFHIPSVKVTGGQRIDMLGVRKEDLPAVWADLGRAGFISGHAYAKGLRTVKT
CVGSDWCRFGTQDSTGLGIRIEKFMWGAWTPAKVKMAVSGCPRNCAEATCKDVGVICVDSGYEIHFAGAAGLDIRGTEVL
GLVKTEDDALEHIVALVQMYREQAHYLERIYKWAKRVGLDEIRRQILDDPQKRKAYFDRFAFSRKFAQVDPWSERVSGKD
KHEFRPMATIGFPVAAEEAL

Sequences:

>Translated_820_residues
MNKKEKLVIVGAGMASGRMLEHLFEADPERFDVTLFGAEPRGNYNRIMLSPVLAGEKSFDQIVTHDAAWYDAHQVECRFG
ETVTRIDRAAKVVHSAGSEAPYDKLVIATGSAPFMIPVAGRDLPGVMAFRDYDDVQAMVAAAGKKDAKAVVIGGGLLGLE
AAAALRLHGMEVVVLHLMGHLMERQLDAAAGYLLQKELESRGIKIHCKAQTSAILGRGRTEAVLLDDGTLYPADIVVMAV
GIRPEARIATDAGLHVERGIVVDDRMLTSDPDILALGECCEHNNICYGLVAPLYDMAKVAAKTLTGVEATFRSVDTATQL
KVTGVSLYSAGDFADAPDREDIVLRNSGAGIYKRLVLKDNRIVGVVLYGDTTDGAWYFDMLRKGTDVSELRETLIFGQSR
QGNASLDPMAAVAALPDDAEICGCNGVCKGAIVSAIIARDLTSLDDVRAHTKASASCGSCTGLVEQLMTLTLGDKYNPDA
VQPVCGCTTLGHGDVRRLIKAKRLKTIPAVMQELEWKTSCGCAKCRPALNYYLVCDWPDEYADDYQSRFVNERVHANIQK
DGTYSVVPRMWGGVTSAKELRAIADVVDKFHIPSVKVTGGQRIDMLGVRKEDLPAVWADLGRAGFISGHAYAKGLRTVKT
CVGSDWCRFGTQDSTGLGIRIEKFMWGAWTPAKVKMAVSGCPRNCAEATCKDVGVICVDSGYEIHFAGAAGLDIRGTEVL
GLVKTEDDALEHIVALVQMYREQAHYLERIYKWAKRVGLDEIRRQILDDPQKRKAYFDRFAFSRKFAQVDPWSERVSGKD
KHEFRPMATIGFPVAAEEAL
>Mature_820_residues
MNKKEKLVIVGAGMASGRMLEHLFEADPERFDVTLFGAEPRGNYNRIMLSPVLAGEKSFDQIVTHDAAWYDAHQVECRFG
ETVTRIDRAAKVVHSAGSEAPYDKLVIATGSAPFMIPVAGRDLPGVMAFRDYDDVQAMVAAAGKKDAKAVVIGGGLLGLE
AAAALRLHGMEVVVLHLMGHLMERQLDAAAGYLLQKELESRGIKIHCKAQTSAILGRGRTEAVLLDDGTLYPADIVVMAV
GIRPEARIATDAGLHVERGIVVDDRMLTSDPDILALGECCEHNNICYGLVAPLYDMAKVAAKTLTGVEATFRSVDTATQL
KVTGVSLYSAGDFADAPDREDIVLRNSGAGIYKRLVLKDNRIVGVVLYGDTTDGAWYFDMLRKGTDVSELRETLIFGQSR
QGNASLDPMAAVAALPDDAEICGCNGVCKGAIVSAIIARDLTSLDDVRAHTKASASCGSCTGLVEQLMTLTLGDKYNPDA
VQPVCGCTTLGHGDVRRLIKAKRLKTIPAVMQELEWKTSCGCAKCRPALNYYLVCDWPDEYADDYQSRFVNERVHANIQK
DGTYSVVPRMWGGVTSAKELRAIADVVDKFHIPSVKVTGGQRIDMLGVRKEDLPAVWADLGRAGFISGHAYAKGLRTVKT
CVGSDWCRFGTQDSTGLGIRIEKFMWGAWTPAKVKMAVSGCPRNCAEATCKDVGVICVDSGYEIHFAGAAGLDIRGTEVL
GLVKTEDDALEHIVALVQMYREQAHYLERIYKWAKRVGLDEIRRQILDDPQKRKAYFDRFAFSRKFAQVDPWSERVSGKD
KHEFRPMATIGFPVAAEEAL

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI21389617, Length=284, Percent_Identity=27.4647887323944, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI65787454, Length=284, Percent_Identity=27.4647887323944, Blast_Score=89, Evalue=1e-17,
Organism=Homo sapiens, GI226437568, Length=284, Percent_Identity=27.4647887323944, Blast_Score=89, Evalue=2e-17,
Organism=Escherichia coli, GI1789765, Length=808, Percent_Identity=35.1485148514851, Blast_Score=437, Evalue=1e-123,
Organism=Escherichia coli, GI1789065, Length=329, Percent_Identity=30.3951367781155, Blast_Score=131, Evalue=2e-31,
Organism=Escherichia coli, GI1788892, Length=280, Percent_Identity=27.5, Blast_Score=82, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17559934, Length=415, Percent_Identity=22.1686746987952, Blast_Score=87, Evalue=4e-17,
Organism=Drosophila melanogaster, GI281359715, Length=240, Percent_Identity=25.8333333333333, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI281359713, Length=240, Percent_Identity=25.8333333333333, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24639250, Length=240, Percent_Identity=25.8333333333333, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI18543267, Length=240, Percent_Identity=25.8333333333333, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24639252, Length=240, Percent_Identity=25.8333333333333, Blast_Score=85, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24639257, Length=240, Percent_Identity=25.8333333333333, Blast_Score=84, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 89381; Mature: 89381

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS00365 NIR_SIR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKKEKLVIVGAGMASGRMLEHLFEADPERFDVTLFGAEPRGNYNRIMLSPVLAGEKSFD
CCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEEECCCCCHHH
QIVTHDAAWYDAHQVECRFGETVTRIDRAAKVVHSAGSEAPYDKLVIATGSAPFMIPVAG
HHHHCCCHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEECC
RDLPGVMAFRDYDDVQAMVAAAGKKDAKAVVIGGGLLGLEAAAALRLHGMEVVVLHLMGH
CCCCCEEEECCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHCCHHHHHHHHHHH
LMERQLDAAAGYLLQKELESRGIKIHCKAQTSAILGRGRTEAVLLDDGTLYPADIVVMAV
HHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHCCCCCCEEEEECCCCCCCEEEEEEE
GIRPEARIATDAGLHVERGIVVDDRMLTSDPDILALGECCEHNNICYGLVAPLYDMAKVA
CCCCCCCEECCCCCEEECCEEEECCEECCCCCEEEECHHHCCCCEEEHHHHHHHHHHHHH
AKTLTGVEATFRSVDTATQLKVTGVSLYSAGDFADAPDREDIVLRNSGAGIYKRLVLKDN
HHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCCCCEEEECCCCCHHHHEEEECC
RIVGVVLYGDTTDGAWYFDMLRKGTDVSELRETLIFGQSRQGNASLDPMAAVAALPDDAE
CEEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCC
ICGCNGVCKGAIVSAIIARDLTSLDDVRAHTKASASCGSCTGLVEQLMTLTLGDKYNPDA
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCC
VQPVCGCTTLGHGDVRRLIKAKRLKTIPAVMQELEWKTSCGCAKCRPALNYYLVCDWPDE
CCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCEEEEECCCHH
YADDYQSRFVNERVHANIQKDGTYSVVPRMWGGVTSAKELRAIADVVDKFHIPSVKVTGG
HHHHHHHHHHHHHHHCCCCCCCCEEECHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECCC
QRIDMLGVRKEDLPAVWADLGRAGFISGHAYAKGLRTVKTCVGSDWCRFGTQDSTGLGIR
CEEEEECCCCHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCEE
IEKFMWGAWTPAKVKMAVSGCPRNCAEATCKDVGVICVDSGYEIHFAGAAGLDIRGTEVL
EEEEECCCCCCCEEEEEECCCCCHHHHHHHHCCCEEEECCCCEEEEECCCCCCCCCCEEE
GLVKTEDDALEHIVALVQMYREQAHYLERIYKWAKRVGLDEIRRQILDDPQKRKAYFDRF
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHH
AFSRKFAQVDPWSERVSGKDKHEFRPMATIGFPVAAEEAL
HHHHHHCCCCCHHHHCCCCCCHHCCCEEEECCCCCHHCCC
>Mature Secondary Structure
MNKKEKLVIVGAGMASGRMLEHLFEADPERFDVTLFGAEPRGNYNRIMLSPVLAGEKSFD
CCCCCEEEEEECCCCCHHHHHHHHHCCCCCEEEEEEECCCCCCCCEEEEEEECCCCCHHH
QIVTHDAAWYDAHQVECRFGETVTRIDRAAKVVHSAGSEAPYDKLVIATGSAPFMIPVAG
HHHHCCCHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEEEEECC
RDLPGVMAFRDYDDVQAMVAAAGKKDAKAVVIGGGLLGLEAAAALRLHGMEVVVLHLMGH
CCCCCEEEECCHHHHHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHCCHHHHHHHHHHH
LMERQLDAAAGYLLQKELESRGIKIHCKAQTSAILGRGRTEAVLLDDGTLYPADIVVMAV
HHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHCCCCCCEEEEECCCCCCCEEEEEEE
GIRPEARIATDAGLHVERGIVVDDRMLTSDPDILALGECCEHNNICYGLVAPLYDMAKVA
CCCCCCCEECCCCCEEECCEEEECCEECCCCCEEEECHHHCCCCEEEHHHHHHHHHHHHH
AKTLTGVEATFRSVDTATQLKVTGVSLYSAGDFADAPDREDIVLRNSGAGIYKRLVLKDN
HHHHHHHHHHHHHCCCCCEEEEEEEEEEECCCCCCCCCCCCEEEECCCCCHHHHEEEECC
RIVGVVLYGDTTDGAWYFDMLRKGTDVSELRETLIFGQSRQGNASLDPMAAVAALPDDAE
CEEEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHCCCCCCC
ICGCNGVCKGAIVSAIIARDLTSLDDVRAHTKASASCGSCTGLVEQLMTLTLGDKYNPDA
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCC
VQPVCGCTTLGHGDVRRLIKAKRLKTIPAVMQELEWKTSCGCAKCRPALNYYLVCDWPDE
CCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCEEEEECCCHH
YADDYQSRFVNERVHANIQKDGTYSVVPRMWGGVTSAKELRAIADVVDKFHIPSVKVTGG
HHHHHHHHHHHHHHHCCCCCCCCEEECHHHHCCCCCHHHHHHHHHHHHHHCCCEEEECCC
QRIDMLGVRKEDLPAVWADLGRAGFISGHAYAKGLRTVKTCVGSDWCRFGTQDSTGLGIR
CEEEEECCCCHHHHHHHHHCCCCCEECCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCEE
IEKFMWGAWTPAKVKMAVSGCPRNCAEATCKDVGVICVDSGYEIHFAGAAGLDIRGTEVL
EEEEECCCCCCCEEEEEECCCCCHHHHHHHHCCCEEEECCCCEEEEECCCCCCCCCCEEE
GLVKTEDDALEHIVALVQMYREQAHYLERIYKWAKRVGLDEIRRQILDDPQKRKAYFDRF
EEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHHHH
AFSRKFAQVDPWSERVSGKDKHEFRPMATIGFPVAAEEAL
HHHHHHCCCCCHHHHCCCCCCHHCCCEEEECCCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]