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The map label for this gene is metB [H]

Identifier: 75674790

GI number: 75674790

Start: 670769

End: 671953

Strand: Direct

Name: metB [H]

Synonym: Nwi_0592

Alternate gene names: 75674790

Gene position: 670769-671953 (Clockwise)

Preceding gene: 75674789

Following gene: 75674801

Centisome position: 19.72

GC content: 62.62

Gene sequence:

>1185_bases
ATGTCCGAATCCATGAATCCGAGAACTGTCGCGGCCGCAAACGGCATCGCGGCGGAAGATACTTTCGGAGCGGTGACGCC
GCCGATCTGTCTGTCGAGCACTTTCACCTGGCCCGGATTCGGGCGCAAGGGTCCGCACGATTATACCCGCACCAGTAATC
CCACGCGGACGCTGCTGGCGGATACCCTCGCCAAGCTCGAGGGTGGGGCGGGCGCGGTGGTGGTGTCCTCGGGCATGGCG
GCGATCGATCTGGTGCTGTCGCAACTCTCGAGCGACGATATCGTGATCGTGCCGCATGATTGTTACGGCGGCACTCACCG
TCTGCTTCTGAACCGCCGTGACCGGGGTCACTTCAAGGTGCGCTTTGTTGATCAGAACGATCAGGCGGCGCTGGCGTCCG
CCTTGAAGGAAAAGCCCAGGCTCGTCCTGATCGAGACCCCGAGCAATCCCCTGATGCGCATCGTCGATATCCGGGCGATT
GTCGCTCAGGCGAGAACGGTCGGCGCCAAGGTTGCCGTTGACAATACCTTTCTGTCGCCCGCGTTGCAGCGGCCGATCGC
GCTGGGCGCCGATTTCGTCATTCATTCAACCACGAAGTATCTCAACGGCCATTCGGACATCGTCGGCGGCGCCGTGATCG
CAGCCGATCCCGCTGACGTTGAGGCGTTGGAGACCTGGGCCAATATCGTTGGCACGACGGGAGCGCCGTTCGATTCCTTC
CTGGCGCTTCGAGGCATCCGCACGCTGTTTCCCCGCGTGGAGTGCCAGCAACGCAATGCGCAAGCAGTTGCCGAGTTTCT
CCTTTCTCATCCTCTTGTCGCCGCCTGTCACTATCCGGGGCTGCCGTCGCATCCGGGGCATGCCGTCGCCAAAGCCCAGC
AATCCGGCTTTGGCGCGATGCTCAGTTTCGAACTGAAAGGAGGCGAGGTGGAAGCTGGCCGCTTCGTCGAGGCGGTGCGG
CTTTTCTGTTTCGCGGAATCGCTCGGCGGCACCGAGAGCCTGGTGGCTCACCCGGCCACGATGACCCATGCCGGAATGTC
GGACGAGGCGCGCAAGGCCGCCGGGATCACCAGCGGTCTTATCCGGCTTTCGATCGGACTGGAGGATGCCGCTGATCTGA
TCGCCGATCTTGATCAGGCTCTTGCCGCGATCGATCGATCGGTTCGATCAAAGGCGAGCGCCTGA

Upstream 100 bases:

>100_bases
TCGACGGAAGTGCTTTAAATAGGCCGAGACCGGCCGGCCGTGATATCCTGAACGGAATCGTCGCGGGTCCTCGCATTTTC
ATCGAAAGTCCACGAACGTT

Downstream 100 bases:

>100_bases
ATCGCGACGTCCGTCCTCAGGACAAACACGCAAGAAAAACGCGGAGCCGGTTGGCTCCGCGGCAGATCACTCGGACGCGA
GCGGCGATATCGCATTTTCG

Product: O-succinylhomoserine (thiol)-lyase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 394; Mature: 393

Protein sequence:

>394_residues
MSESMNPRTVAAANGIAAEDTFGAVTPPICLSSTFTWPGFGRKGPHDYTRTSNPTRTLLADTLAKLEGGAGAVVVSSGMA
AIDLVLSQLSSDDIVIVPHDCYGGTHRLLLNRRDRGHFKVRFVDQNDQAALASALKEKPRLVLIETPSNPLMRIVDIRAI
VAQARTVGAKVAVDNTFLSPALQRPIALGADFVIHSTTKYLNGHSDIVGGAVIAADPADVEALETWANIVGTTGAPFDSF
LALRGIRTLFPRVECQQRNAQAVAEFLLSHPLVAACHYPGLPSHPGHAVAKAQQSGFGAMLSFELKGGEVEAGRFVEAVR
LFCFAESLGGTESLVAHPATMTHAGMSDEARKAAGITSGLIRLSIGLEDAADLIADLDQALAAIDRSVRSKASA

Sequences:

>Translated_394_residues
MSESMNPRTVAAANGIAAEDTFGAVTPPICLSSTFTWPGFGRKGPHDYTRTSNPTRTLLADTLAKLEGGAGAVVVSSGMA
AIDLVLSQLSSDDIVIVPHDCYGGTHRLLLNRRDRGHFKVRFVDQNDQAALASALKEKPRLVLIETPSNPLMRIVDIRAI
VAQARTVGAKVAVDNTFLSPALQRPIALGADFVIHSTTKYLNGHSDIVGGAVIAADPADVEALETWANIVGTTGAPFDSF
LALRGIRTLFPRVECQQRNAQAVAEFLLSHPLVAACHYPGLPSHPGHAVAKAQQSGFGAMLSFELKGGEVEAGRFVEAVR
LFCFAESLGGTESLVAHPATMTHAGMSDEARKAAGITSGLIRLSIGLEDAADLIADLDQALAAIDRSVRSKASA
>Mature_393_residues
SESMNPRTVAAANGIAAEDTFGAVTPPICLSSTFTWPGFGRKGPHDYTRTSNPTRTLLADTLAKLEGGAGAVVVSSGMAA
IDLVLSQLSSDDIVIVPHDCYGGTHRLLLNRRDRGHFKVRFVDQNDQAALASALKEKPRLVLIETPSNPLMRIVDIRAIV
AQARTVGAKVAVDNTFLSPALQRPIALGADFVIHSTTKYLNGHSDIVGGAVIAADPADVEALETWANIVGTTGAPFDSFL
ALRGIRTLFPRVECQQRNAQAVAEFLLSHPLVAACHYPGLPSHPGHAVAKAQQSGFGAMLSFELKGGEVEAGRFVEAVRL
FCFAESLGGTESLVAHPATMTHAGMSDEARKAAGITSGLIRLSIGLEDAADLIADLDQALAAIDRSVRSKASA

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=361, Percent_Identity=42.6592797783933, Blast_Score=260, Evalue=2e-69,
Organism=Homo sapiens, GI299473759, Length=361, Percent_Identity=39.8891966759003, Blast_Score=226, Evalue=3e-59,
Organism=Homo sapiens, GI25453487, Length=360, Percent_Identity=37.5, Blast_Score=206, Evalue=3e-53,
Organism=Escherichia coli, GI1790375, Length=379, Percent_Identity=55.9366754617414, Blast_Score=433, Evalue=1e-123,
Organism=Escherichia coli, GI1789383, Length=390, Percent_Identity=31.2820512820513, Blast_Score=148, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI17538055, Length=363, Percent_Identity=45.1790633608815, Blast_Score=286, Evalue=1e-77,
Organism=Caenorhabditis elegans, GI17560056, Length=360, Percent_Identity=43.3333333333333, Blast_Score=275, Evalue=2e-74,
Organism=Caenorhabditis elegans, GI71980708, Length=371, Percent_Identity=34.5013477088949, Blast_Score=214, Evalue=6e-56,
Organism=Saccharomyces cerevisiae, GI6319307, Length=372, Percent_Identity=39.7849462365591, Blast_Score=259, Evalue=4e-70,
Organism=Saccharomyces cerevisiae, GI6321254, Length=408, Percent_Identity=34.3137254901961, Blast_Score=193, Evalue=5e-50,
Organism=Saccharomyces cerevisiae, GI6323333, Length=393, Percent_Identity=34.0966921119593, Blast_Score=151, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6321904, Length=394, Percent_Identity=29.9492385786802, Blast_Score=133, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6321170, Length=315, Percent_Identity=29.5238095238095, Blast_Score=112, Evalue=1e-25,
Organism=Drosophila melanogaster, GI19922554, Length=360, Percent_Identity=43.3333333333333, Blast_Score=298, Evalue=5e-81,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR011821
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 41492; Mature: 41360

Theoretical pI: Translated: 6.65; Mature: 6.65

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESMNPRTVAAANGIAAEDTFGAVTPPICLSSTFTWPGFGRKGPHDYTRTSNPTRTLLA
CCCCCCCCEEEECCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
DTLAKLEGGAGAVVVSSGMAAIDLVLSQLSSDDIVIVPHDCYGGTHRLLLNRRDRGHFKV
HHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCCCCEEEE
RFVDQNDQAALASALKEKPRLVLIETPSNPLMRIVDIRAIVAQARTVGAKVAVDNTFLSP
EEECCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEECCHHHCH
ALQRPIALGADFVIHSTTKYLNGHSDIVGGAVIAADPADVEALETWANIVGTTGAPFDSF
HHHCCHHHCCCEEEECHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCHHHH
LALRGIRTLFPRVECQQRNAQAVAEFLLSHPLVAACHYPGLPSHPGHAVAKAQQSGFGAM
HHHHHHHHHHCCHHHHHCCHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHCCCCEE
LSFELKGGEVEAGRFVEAVRLFCFAESLGGTESLVAHPATMTHAGMSDEARKAAGITSGL
EEEEECCCEECHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHCCCCCHHHHHHHCHHHCE
IRLSIGLEDAADLIADLDQALAAIDRSVRSKASA
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SESMNPRTVAAANGIAAEDTFGAVTPPICLSSTFTWPGFGRKGPHDYTRTSNPTRTLLA
CCCCCCCEEEECCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHH
DTLAKLEGGAGAVVVSSGMAAIDLVLSQLSSDDIVIVPHDCYGGTHRLLLNRRDRGHFKV
HHHHHHCCCCCEEEEECCHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEECCCCCEEEE
RFVDQNDQAALASALKEKPRLVLIETPSNPLMRIVDIRAIVAQARTVGAKVAVDNTFLSP
EEECCCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEECCHHHCH
ALQRPIALGADFVIHSTTKYLNGHSDIVGGAVIAADPADVEALETWANIVGTTGAPFDSF
HHHCCHHHCCCEEEECHHHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHCCCCCCHHHH
LALRGIRTLFPRVECQQRNAQAVAEFLLSHPLVAACHYPGLPSHPGHAVAKAQQSGFGAM
HHHHHHHHHHCCHHHHHCCHHHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHCCCCEE
LSFELKGGEVEAGRFVEAVRLFCFAESLGGTESLVAHPATMTHAGMSDEARKAAGITSGL
EEEEECCCEECHHHHHHHHHHHHHHHHCCCCHHHHCCCCHHHCCCCCHHHHHHHCHHHCE
IRLSIGLEDAADLIADLDQALAAIDRSVRSKASA
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6361020; 8346018; 9278503; 3307782; 9843488 [H]