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The map label for this gene is rfbD [H]

Identifier: 75674745

GI number: 75674745

Start: 612965

End: 613891

Strand: Direct

Name: rfbD [H]

Synonym: Nwi_0547

Alternate gene names: 75674745

Gene position: 612965-613891 (Clockwise)

Preceding gene: 75674744

Following gene: 75674750

Centisome position: 18.02

GC content: 64.4

Gene sequence:

>927_bases
ATGCGGATACTGTTGACGGGTAGTAGCGGTCAGGTCGGCGGAGCGCTGCGTCCGCTGCTGAAGGAAGGCGGCATGGTCAT
CGCGCCGTCGCGCAGCGCGTTCGACCTGTCGAATCCCGAAACGCTCGCCGGGGCGCTCGACCGATTCAAACCCGATCTCA
TCATCAATCCCGCGGCCTATACCGCGGTGGATCGCGCGGAAGACGAGCGTGAACTCGCTTTCCTCGTCAACGCCAAAGCA
CCCGCTGCGATCGCGGAATGGTCGGCACGGCATCGTGTGCCGTTGATTCATTTCTCCACCGACTACGTCTTCGATGGCTC
TGGCGACAAGCCGTGGCGTGAGGACAGTCCCACGGGTCCTCTCTCGGTCTATGGCGCCAGCAAGCTCGCCGGCGATCTCG
CCATCGAGGCGGCCGGCGGGCCGCATCTGATCGCGCGCACCTCGTGGGTCTATGCCGCGAGCGGAGCCAATTTCCTGCGC
ACGATCGCGCGGCTGGCCGGCGAACGCAAGGAGTTGCGCATTGTCGCCGATCAGACAGGCGCTCCGACCACGGCAAACAC
GATCGCCGATGCCGTGTCTCGCATCGTGCTCTCGAATGCATCGGATTTGAGCGCGTTGTTCGCGCGCAGCGGCGGCGTGG
TCAATCTCGCCTGTGCGGGCGAAACCAGTTGGCACGGCTTCGCCACCGCCATTGTCGGCGGATTGAAGTCGCGCGGTGCG
GGACTGTCTGTGGAGACGATCATCCCGATCGCGACGGCGGATTTTCCGACCAGGGCAAGGCGGCCTGGCAATTCCAGGCT
CGACCTGTCGCAACTCCGAGAACGTTTCGGATTAACTCCGCCAACGTGGCAGGACGCGCTGTCGACGGAACTTGATCGCC
TCGTCACCGGGCAGGACGAACAGGCGGTCCCCGCAATGCGAGCATGA

Upstream 100 bases:

>100_bases
GCGTGGAGACGCCGTCCTTGTCCGCCAAGGACGCGGACGCTCCGCTGCTGGCGGACGTAAAGAACTTTCCAATCCATGGG
CAGGTGTGATGGCAAGTCTG

Downstream 100 bases:

>100_bases
TCTCGAGACGACGCTGCGCTGTAGCGTTTTCGAGCGAAGCGGATACCGGTTCGCGTGAAGAAAACGCATCAAAATCCCAA
CGGCAGATCGTGCGCGACCG

Product: dTDP-4-dehydrorhamnose reductase

Products: NA

Alternate protein names: dTDP-4-keto-L-rhamnose reductase; dTDP-6-deoxy-L-lyxo-4-hexulose reductase; dTDP-6-deoxy-L-mannose dehydrogenase; dTDP-L-rhamnose synthase [H]

Number of amino acids: Translated: 308; Mature: 308

Protein sequence:

>308_residues
MRILLTGSSGQVGGALRPLLKEGGMVIAPSRSAFDLSNPETLAGALDRFKPDLIINPAAYTAVDRAEDERELAFLVNAKA
PAAIAEWSARHRVPLIHFSTDYVFDGSGDKPWREDSPTGPLSVYGASKLAGDLAIEAAGGPHLIARTSWVYAASGANFLR
TIARLAGERKELRIVADQTGAPTTANTIADAVSRIVLSNASDLSALFARSGGVVNLACAGETSWHGFATAIVGGLKSRGA
GLSVETIIPIATADFPTRARRPGNSRLDLSQLRERFGLTPPTWQDALSTELDRLVTGQDEQAVPAMRA

Sequences:

>Translated_308_residues
MRILLTGSSGQVGGALRPLLKEGGMVIAPSRSAFDLSNPETLAGALDRFKPDLIINPAAYTAVDRAEDERELAFLVNAKA
PAAIAEWSARHRVPLIHFSTDYVFDGSGDKPWREDSPTGPLSVYGASKLAGDLAIEAAGGPHLIARTSWVYAASGANFLR
TIARLAGERKELRIVADQTGAPTTANTIADAVSRIVLSNASDLSALFARSGGVVNLACAGETSWHGFATAIVGGLKSRGA
GLSVETIIPIATADFPTRARRPGNSRLDLSQLRERFGLTPPTWQDALSTELDRLVTGQDEQAVPAMRA
>Mature_308_residues
MRILLTGSSGQVGGALRPLLKEGGMVIAPSRSAFDLSNPETLAGALDRFKPDLIINPAAYTAVDRAEDERELAFLVNAKA
PAAIAEWSARHRVPLIHFSTDYVFDGSGDKPWREDSPTGPLSVYGASKLAGDLAIEAAGGPHLIARTSWVYAASGANFLR
TIARLAGERKELRIVADQTGAPTTANTIADAVSRIVLSNASDLSALFARSGGVVNLACAGETSWHGFATAIVGGLKSRGA
GLSVETIIPIATADFPTRARRPGNSRLDLSQLRERFGLTPPTWQDALSTELDRLVTGQDEQAVPAMRA

Specific function: Catalyzes the reduction of dTDP-6-deoxy-L-lyxo-4- hexulose to yield dTDP-L-rhamnose. RmlD uses NADH and NADPH nearly equally well [H]

COG id: COG1091

COG function: function code M; dTDP-4-dehydrorhamnose reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dTDP-4-dehydrorhamnose reductase family [H]

Homologues:

Organism=Escherichia coli, GI1788352, Length=303, Percent_Identity=41.9141914191419, Blast_Score=227, Evalue=8e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005913
- InterPro:   IPR016040 [H]

Pfam domain/function: PF04321 RmlD_sub_bind [H]

EC number: =1.1.1.133 [H]

Molecular weight: Translated: 32526; Mature: 32526

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILLTGSSGQVGGALRPLLKEGGMVIAPSRSAFDLSNPETLAGALDRFKPDLIINPAAY
CEEEEECCCCCCCHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCEEECCHHH
TAVDRAEDERELAFLVNAKAPAAIAEWSARHRVPLIHFSTDYVFDGSGDKPWREDSPTGP
HHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEEECCEEEECCCCCCCCCCCCCCC
LSVYGASKLAGDLAIEAAGGPHLIARTSWVYAASGANFLRTIARLAGERKELRIVADQTG
EEECCHHHHCCCEEEEECCCCEEEEEEEEEEEECCHHHHHHHHHHHCCCCEEEEEECCCC
APTTANTIADAVSRIVLSNASDLSALFARSGGVVNLACAGETSWHGFATAIVGGLKSRGA
CCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCC
GLSVETIIPIATADFPTRARRPGNSRLDLSQLRERFGLTPPTWQDALSTELDRLVTGQDE
CCEEEEEEEEEECCCCHHHCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCC
QAVPAMRA
CCCCCCCC
>Mature Secondary Structure
MRILLTGSSGQVGGALRPLLKEGGMVIAPSRSAFDLSNPETLAGALDRFKPDLIINPAAY
CEEEEECCCCCCCHHHHHHHHCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCEEECCHHH
TAVDRAEDERELAFLVNAKAPAAIAEWSARHRVPLIHFSTDYVFDGSGDKPWREDSPTGP
HHHHHCCCCCEEEEEEECCCCHHHHHHHHHCCCEEEEEECCEEEECCCCCCCCCCCCCCC
LSVYGASKLAGDLAIEAAGGPHLIARTSWVYAASGANFLRTIARLAGERKELRIVADQTG
EEECCHHHHCCCEEEEECCCCEEEEEEEEEEEECCHHHHHHHHHHHCCCCEEEEEECCCC
APTTANTIADAVSRIVLSNASDLSALFARSGGVVNLACAGETSWHGFATAIVGGLKSRGA
CCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCC
GLSVETIIPIATADFPTRARRPGNSRLDLSQLRERFGLTPPTWQDALSTELDRLVTGQDE
CCEEEEEEEEEECCCCHHHCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCC
QAVPAMRA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1710759; 11677609 [H]