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The map label for this gene is lig [H]

Identifier: 75674660

GI number: 75674660

Start: 518793

End: 520577

Strand: Direct

Name: lig [H]

Synonym: Nwi_0462

Alternate gene names: 75674660

Gene position: 518793-520577 (Clockwise)

Preceding gene: 75674659

Following gene: 75674661

Centisome position: 15.25

GC content: 65.55

Gene sequence:

>1785_bases
GTGAATCGCTTCGCCGAACTTCTCGACCGTCTCGCCTATGAGCCCGGCCGCAACAACAAGCTGCGGCTGATGACGGCCTA
TTTTCGCGAGACGCCGGACCCGGATCGGGGCTACGCACTGTCCGCGCTCACCGGCGCGTTGTCGTTCAAGTACGCCAAGT
CAGGTCTGGTGCGCGACCTGATCGCCGCGCGCGCCGATCCGGTGCTGTTCGCGTTTTCCTATGATTATGTCGGCGATCTT
TCCGAGACGGTGGCGCTCATGTGGCCACAACAGCACCCTCCCGACGCCTCGTCCGTTCTCTCTGCCGCTCGCGGGAGCGG
GACTGGGAAGGCAACGGGCACAGGTCACGTTCGTCATTGGCCTTCTCCGACTCTTACCGACGTCGTCACCACGCTGCGCA
CGCTCGGCAAGGCCGAACTGCCGGCGCAACTTGCGCGCTGGCTCGACCAACTCGACGAGACCGGACGCTGGGCGCTCTTG
AAACTCGTCACCGGCGCGATGCGGATCGGGGTCTCCGCGCGGCTCGCCAAGACCGCCGTGGCCGCGCTTGGGAGCAGGGA
TGCGCATGATGTCGAACTGATCTGGCCGGGCCTCGCGCCGCCTTATCCCGATCTGTTCGCCTGGCTTGAGGGCCGCGGCG
ACAAGCCGGTCAATCGCGCTCCCGCGCCGTTCCGGCCGGTGATGCTGGCGCACGCGATCGAAGATGCTGACTTTGCGCGC
CTGGATGCCACCGATTTCACCGCCGAGTGGAAGTGGGACGGCATCCGGGTGCAGGCGGTGAGGGGCCGCGATGAGCACGG
CAATGTCGAGACGCGGCTTTATTCGCGCACCGGCGAGGACATCACGCACAGTTTCCCGGATCTGCTGCCCTCGTTGCGGC
GCGAAGGTTTTGCCGAAGGATGGCTCAACGACTCGCGGGAACCGGTCCGCGGTTTTCGTCCGCGGGCTTTCGCCATCGAT
GGCGAACTGCTGGTGGTGCGCGATGGACGGGTGCAGTCGTTCAACGTCCTGCAACAGAGGCTGAACCGCAAGGCGGTGAC
GCCCAGGCTGATGAAGGAGTTCCCCGTTCGCCTGCGCGCCTACGATCTGCTCGCCGACGGCGACAATGATCTGCGCGAGT
TGCCGTTTGAAGACCGGCGGCGGCGGCTTGTTGATTTCATCGCCGCGCTTGACGACCCGCGTATCGACCTTTCGCCGCAG
GTCTCCTTCGCCACATGGAGCGATCTCGCGGCGATCCGCGCCGATCCGGCCGCGGCGGGCGCTGACGCCGAGGCTGTCGA
AGGCATCATGTTGAAGCGCCGGGACGCGCCTTATATGCCGGGGCGCCCCAAGGGTCTGTGGTGGAAATGGAAGCGTGATC
CGCATGTCATCGACGCGGTGCTGATGTATGCGCAGCGCGGCCACGGCAGGCGCTCGTCCTATTACTCCGACTACACCTTC
GGAGTCTGGACCGCGGGAGAGGAGGGCGAGCGGCTGGTGCCTGTCGGCAAGGCCTATTTCGGCTTCACCGACGAGGAGCT
GCTGCGGATCGACCGTTTCGTCCGCCGCAACACCACCGAGAGTTTCGGCCCGGTGCGCCATGTCCTGCATGAGCCCGATC
AGGGGTTGGTGCTGGAAATCGCCTTTGAGGGCTTGCAGCGTTCGGCCCGGCACAAGTCGGGGGTGGCGATGCGTTTTCCG
CGGATCAACCGATTGCGCTGGGACAAGCCACCGCGCGAGGCGGATCGGCTGGAGACGCTGGAGCGGATGCTGGCTTCCGA
AGCCACCAGATCAAACGGCCGTTGA

Upstream 100 bases:

>100_bases
GGGCGCAAGGTCTCGCCGCGCGCCCGCTGGCATTGGTCGGCTATGGCGATGAGGACGGCGAGCAGGATGCGGCGGCTTTC
TCCGGCGGAGAGGACGGCTC

Downstream 100 bases:

>100_bases
CCGCAGAACGGTGGTTCCCCATCTGCCCCACACTGATTATGTTGCGCGGGTCCTTGTGCCTGCAGGAGAGCTTTGATGCC
GAACGACGACACCCGGAATT

Product: ATP-dependent DNA ligase

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [ATP] [H]

Number of amino acids: Translated: 594; Mature: 594

Protein sequence:

>594_residues
MNRFAELLDRLAYEPGRNNKLRLMTAYFRETPDPDRGYALSALTGALSFKYAKSGLVRDLIAARADPVLFAFSYDYVGDL
SETVALMWPQQHPPDASSVLSAARGSGTGKATGTGHVRHWPSPTLTDVVTTLRTLGKAELPAQLARWLDQLDETGRWALL
KLVTGAMRIGVSARLAKTAVAALGSRDAHDVELIWPGLAPPYPDLFAWLEGRGDKPVNRAPAPFRPVMLAHAIEDADFAR
LDATDFTAEWKWDGIRVQAVRGRDEHGNVETRLYSRTGEDITHSFPDLLPSLRREGFAEGWLNDSREPVRGFRPRAFAID
GELLVVRDGRVQSFNVLQQRLNRKAVTPRLMKEFPVRLRAYDLLADGDNDLRELPFEDRRRRLVDFIAALDDPRIDLSPQ
VSFATWSDLAAIRADPAAAGADAEAVEGIMLKRRDAPYMPGRPKGLWWKWKRDPHVIDAVLMYAQRGHGRRSSYYSDYTF
GVWTAGEEGERLVPVGKAYFGFTDEELLRIDRFVRRNTTESFGPVRHVLHEPDQGLVLEIAFEGLQRSARHKSGVAMRFP
RINRLRWDKPPREADRLETLERMLASEATRSNGR

Sequences:

>Translated_594_residues
MNRFAELLDRLAYEPGRNNKLRLMTAYFRETPDPDRGYALSALTGALSFKYAKSGLVRDLIAARADPVLFAFSYDYVGDL
SETVALMWPQQHPPDASSVLSAARGSGTGKATGTGHVRHWPSPTLTDVVTTLRTLGKAELPAQLARWLDQLDETGRWALL
KLVTGAMRIGVSARLAKTAVAALGSRDAHDVELIWPGLAPPYPDLFAWLEGRGDKPVNRAPAPFRPVMLAHAIEDADFAR
LDATDFTAEWKWDGIRVQAVRGRDEHGNVETRLYSRTGEDITHSFPDLLPSLRREGFAEGWLNDSREPVRGFRPRAFAID
GELLVVRDGRVQSFNVLQQRLNRKAVTPRLMKEFPVRLRAYDLLADGDNDLRELPFEDRRRRLVDFIAALDDPRIDLSPQ
VSFATWSDLAAIRADPAAAGADAEAVEGIMLKRRDAPYMPGRPKGLWWKWKRDPHVIDAVLMYAQRGHGRRSSYYSDYTF
GVWTAGEEGERLVPVGKAYFGFTDEELLRIDRFVRRNTTESFGPVRHVLHEPDQGLVLEIAFEGLQRSARHKSGVAMRFP
RINRLRWDKPPREADRLETLERMLASEATRSNGR
>Mature_594_residues
MNRFAELLDRLAYEPGRNNKLRLMTAYFRETPDPDRGYALSALTGALSFKYAKSGLVRDLIAARADPVLFAFSYDYVGDL
SETVALMWPQQHPPDASSVLSAARGSGTGKATGTGHVRHWPSPTLTDVVTTLRTLGKAELPAQLARWLDQLDETGRWALL
KLVTGAMRIGVSARLAKTAVAALGSRDAHDVELIWPGLAPPYPDLFAWLEGRGDKPVNRAPAPFRPVMLAHAIEDADFAR
LDATDFTAEWKWDGIRVQAVRGRDEHGNVETRLYSRTGEDITHSFPDLLPSLRREGFAEGWLNDSREPVRGFRPRAFAID
GELLVVRDGRVQSFNVLQQRLNRKAVTPRLMKEFPVRLRAYDLLADGDNDLRELPFEDRRRRLVDFIAALDDPRIDLSPQ
VSFATWSDLAAIRADPAAAGADAEAVEGIMLKRRDAPYMPGRPKGLWWKWKRDPHVIDAVLMYAQRGHGRRSSYYSDYTF
GVWTAGEEGERLVPVGKAYFGFTDEELLRIDRFVRRNTTESFGPVRHVLHEPDQGLVLEIAFEGLQRSARHKSGVAMRFP
RINRLRWDKPPREADRLETLERMLASEATRSNGR

Specific function: DNA ligase that seals nicks in double-stranded DNA during DNA replication, DNA recombination and DNA repair [H]

COG id: COG1793

COG function: function code L; ATP-dependent DNA ligase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent DNA ligase family [H]

Homologues:

Organism=Homo sapiens, GI4557719, Length=383, Percent_Identity=27.4151436031332, Blast_Score=78, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71985269, Length=401, Percent_Identity=27.930174563591, Blast_Score=96, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022865
- InterPro:   IPR000977
- InterPro:   IPR012309
- InterPro:   IPR012310
- InterPro:   IPR016059
- InterPro:   IPR012308
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF04679 DNA_ligase_A_C; PF01068 DNA_ligase_A_M; PF04675 DNA_ligase_A_N [H]

EC number: =6.5.1.1 [H]

Molecular weight: Translated: 67001; Mature: 67001

Theoretical pI: Translated: 9.52; Mature: 9.52

Prosite motif: PS00024 HEMOPEXIN ; PS00697 DNA_LIGASE_A1 ; PS50160 DNA_LIGASE_A3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRFAELLDRLAYEPGRNNKLRLMTAYFRETPDPDRGYALSALTGALSFKYAKSGLVRDL
CCHHHHHHHHHHCCCCCCCCEEEEEEHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHH
IAARADPVLFAFSYDYVGDLSETVALMWPQQHPPDASSVLSAARGSGTGKATGTGHVRHW
HHHCCCCEEEEEECHHHCCHHHHHHEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEC
PSPTLTDVVTTLRTLGKAELPAQLARWLDQLDETGRWALLKLVTGAMRIGVSARLAKTAV
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHH
AALGSRDAHDVELIWPGLAPPYPDLFAWLEGRGDKPVNRAPAPFRPVMLAHAIEDADFAR
HHHCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCHHE
LDATDFTAEWKWDGIRVQAVRGRDEHGNVETRLYSRTGEDITHSFPDLLPSLRREGFAEG
ECCCCCCEEEEECCEEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCHHH
WLNDSREPVRGFRPRAFAIDGELLVVRDGRVQSFNVLQQRLNRKAVTPRLMKEFPVRLRA
CCCCCCCCHHCCCCCEEEECCEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHCCCHHEE
YDLLADGDNDLRELPFEDRRRRLVDFIAALDDPRIDLSPQVSFATWSDLAAIRADPAAAG
EEEECCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
ADAEAVEGIMLKRRDAPYMPGRPKGLWWKWKRDPHVIDAVLMYAQRGHGRRSSYYSDYTF
CCHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCEE
GVWTAGEEGERLVPVGKAYFGFTDEELLRIDRFVRRNTTESFGPVRHVLHEPDQGLVLEI
EEEECCCCCCEEEECCHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEHH
AFEGLQRSARHKSGVAMRFPRINRLRWDKPPREADRLETLERMLASEATRSNGR
HHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNRFAELLDRLAYEPGRNNKLRLMTAYFRETPDPDRGYALSALTGALSFKYAKSGLVRDL
CCHHHHHHHHHHCCCCCCCCEEEEEEHHHCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHH
IAARADPVLFAFSYDYVGDLSETVALMWPQQHPPDASSVLSAARGSGTGKATGTGHVRHW
HHHCCCCEEEEEECHHHCCHHHHHHEECCCCCCCCHHHHHHHHCCCCCCCCCCCCCEEEC
PSPTLTDVVTTLRTLGKAELPAQLARWLDQLDETGRWALLKLVTGAMRIGVSARLAKTAV
CCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCHHHHHHHHHH
AALGSRDAHDVELIWPGLAPPYPDLFAWLEGRGDKPVNRAPAPFRPVMLAHAIEDADFAR
HHHCCCCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHCCCCHHE
LDATDFTAEWKWDGIRVQAVRGRDEHGNVETRLYSRTGEDITHSFPDLLPSLRREGFAEG
ECCCCCCEEEEECCEEEEEECCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCHHH
WLNDSREPVRGFRPRAFAIDGELLVVRDGRVQSFNVLQQRLNRKAVTPRLMKEFPVRLRA
CCCCCCCCHHCCCCCEEEECCEEEEEECCCCHHHHHHHHHHHHHHCCHHHHHHCCCHHEE
YDLLADGDNDLRELPFEDRRRRLVDFIAALDDPRIDLSPQVSFATWSDLAAIRADPAAAG
EEEECCCCCHHHHCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
ADAEAVEGIMLKRRDAPYMPGRPKGLWWKWKRDPHVIDAVLMYAQRGHGRRSSYYSDYTF
CCHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCEE
GVWTAGEEGERLVPVGKAYFGFTDEELLRIDRFVRRNTTESFGPVRHVLHEPDQGLVLEI
EEEECCCCCCEEEECCHHHCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCEEEHH
AFEGLQRSARHKSGVAMRFPRINRLRWDKPPREADRLETLERMLASEATRSNGR
HHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA