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The map label for this gene is sucB [H]

Identifier: 75674621

GI number: 75674621

Start: 476388

End: 477662

Strand: Direct

Name: sucB [H]

Synonym: Nwi_0423

Alternate gene names: 75674621

Gene position: 476388-477662 (Clockwise)

Preceding gene: 75674620

Following gene: 75674622

Centisome position: 14.0

GC content: 64.39

Gene sequence:

>1275_bases
ATGACTGAGATACGGGTTCCGACGCTCGGCGAATCGGTGACCGAGGCGACCATCGGAAAATGGTTCAAGAAACAAGGCGA
CGCCGTATCCGTCGACGAGCCGCTCGTCGAACTCGAAACCGACAAGGTAACCATCGAAGTGCCCGCGCCGTCCGCCGGCA
CGCTCGCCGAACTCGTCGCCAGGCAGGGCGAGACGGTGGCCGTCGGCGCGCTGCTCGGACAGATCACCGAAGGCGGCGCG
CCGGCAAAGCCAGCGGCGGCCAAGACGCAAGGGATCAGTGCCGAAAGCACGACCGGGCGGCCGGACCTTAAGTCCGACAC
CACGACACCGATCAACGCTGGCCCGGAAGAACCGCGGCCGAGAGCGGAAGCCGACGCGCCGCTAGCGCCCTCGGTGCGCA
AACTCTCCGCTGAGAGCGGCATCGACGCTTCCACCGTGTCGGGCTCCGGCAAGGATGGCCGCGTCACCAAGGGCGATATG
CTGGCGGCGATCGAGAAGGCTGCGTCGGCGCCGACCCCGGTCGACCAGCCGGCTGCCGCCGTGCAGGTGCGCGCGCCTTC
TCCGGCGGACGACGCCTCGCGCGAGGAGCGCGTGAAGATGACGCGGCTGCGCCAGACCATCGCGCGCCGCCTCAAGGATG
TGCAGAACACCGCCGCGATTCTGACGACCTTCAACGAAGTCGATATGAGCGGCGTCATGGCGCTTCGCGCGCATTACAAG
GACGTGTTCGAGAAGAAGCATGGCGTGAAGCTCGGCTTCATGGGCTTCTTCACCAAGGCCATCGTGCAGGCGTTGAGGGA
CATTCCGGCGGTCAATGCCGAAATCGACGGCAGCGACCTGATCTACAAGAACTACTATCACATCGGAATCGCCGTCGGCA
CCGACAAGGGACTGGTGGTGCCCGTGGTGCGCGACTGCGATCAAAAATCGATCGCCGAGATCGAGAAGAGCGTCGCCGAC
TACGGCCGCCGCGCCCGCGACGGTCAGCTCAAGATAGAGGAAATGCAGGGCGGCACCTTCACCATCACCAACGGCGGCAT
CTACGGCTCGCTGATGTCGACGCCGATCCTGAATGCGCCGCAGGCCGGCATTCTCGGCATGCACAAGATTCAGGAACGGC
CGGTGGCGATCGGCGGCAAGGTGGAAATCCGGCCGATGATGTATCTCGCTCTCTCTTATGATCACCGCGTCATCGACGGC
AAGGAGGCCGTCACCTTCCTTGTGCGCGTCAAGGAATCGCTCGAAGATCCGGCACGGCTGGTGCTGGATCTGTAA

Upstream 100 bases:

>100_bases
CGGACGGTCATGTCGGACTGAAACACGAGGCCAGCTTGTGGCGGATGCTTCCGAGTATCTTTTTTCGGGACAGGTCAGCC
ATGACGAAAGGAAGAGGACC

Downstream 100 bases:

>100_bases
TTCAAGCTAGAGCATGTTCGCTTCTCGTTTAATCAGAAGCGAGGCTCTGGATTATGTTTGGCGCGTTTTCTTCACGCGAA
CAGGTCATCCATCCTCGGGT

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MTEIRVPTLGESVTEATIGKWFKKQGDAVSVDEPLVELETDKVTIEVPAPSAGTLAELVARQGETVAVGALLGQITEGGA
PAKPAAAKTQGISAESTTGRPDLKSDTTTPINAGPEEPRPRAEADAPLAPSVRKLSAESGIDASTVSGSGKDGRVTKGDM
LAAIEKAASAPTPVDQPAAAVQVRAPSPADDASREERVKMTRLRQTIARRLKDVQNTAAILTTFNEVDMSGVMALRAHYK
DVFEKKHGVKLGFMGFFTKAIVQALRDIPAVNAEIDGSDLIYKNYYHIGIAVGTDKGLVVPVVRDCDQKSIAEIEKSVAD
YGRRARDGQLKIEEMQGGTFTITNGGIYGSLMSTPILNAPQAGILGMHKIQERPVAIGGKVEIRPMMYLALSYDHRVIDG
KEAVTFLVRVKESLEDPARLVLDL

Sequences:

>Translated_424_residues
MTEIRVPTLGESVTEATIGKWFKKQGDAVSVDEPLVELETDKVTIEVPAPSAGTLAELVARQGETVAVGALLGQITEGGA
PAKPAAAKTQGISAESTTGRPDLKSDTTTPINAGPEEPRPRAEADAPLAPSVRKLSAESGIDASTVSGSGKDGRVTKGDM
LAAIEKAASAPTPVDQPAAAVQVRAPSPADDASREERVKMTRLRQTIARRLKDVQNTAAILTTFNEVDMSGVMALRAHYK
DVFEKKHGVKLGFMGFFTKAIVQALRDIPAVNAEIDGSDLIYKNYYHIGIAVGTDKGLVVPVVRDCDQKSIAEIEKSVAD
YGRRARDGQLKIEEMQGGTFTITNGGIYGSLMSTPILNAPQAGILGMHKIQERPVAIGGKVEIRPMMYLALSYDHRVIDG
KEAVTFLVRVKESLEDPARLVLDL
>Mature_423_residues
TEIRVPTLGESVTEATIGKWFKKQGDAVSVDEPLVELETDKVTIEVPAPSAGTLAELVARQGETVAVGALLGQITEGGAP
AKPAAAKTQGISAESTTGRPDLKSDTTTPINAGPEEPRPRAEADAPLAPSVRKLSAESGIDASTVSGSGKDGRVTKGDML
AAIEKAASAPTPVDQPAAAVQVRAPSPADDASREERVKMTRLRQTIARRLKDVQNTAAILTTFNEVDMSGVMALRAHYKD
VFEKKHGVKLGFMGFFTKAIVQALRDIPAVNAEIDGSDLIYKNYYHIGIAVGTDKGLVVPVVRDCDQKSIAEIEKSVADY
GRRARDGQLKIEEMQGGTFTITNGGIYGSLMSTPILNAPQAGILGMHKIQERPVAIGGKVEIRPMMYLALSYDHRVIDGK
EAVTFLVRVKESLEDPARLVLDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and l

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=251, Percent_Identity=54.1832669322709, Blast_Score=292, Evalue=5e-79,
Organism=Homo sapiens, GI203098753, Length=461, Percent_Identity=29.5010845986985, Blast_Score=167, Evalue=1e-41,
Organism=Homo sapiens, GI203098816, Length=461, Percent_Identity=29.5010845986985, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI110671329, Length=444, Percent_Identity=28.3783783783784, Blast_Score=160, Evalue=2e-39,
Organism=Homo sapiens, GI31711992, Length=441, Percent_Identity=27.6643990929705, Blast_Score=153, Evalue=3e-37,
Organism=Homo sapiens, GI260898739, Length=167, Percent_Identity=37.7245508982036, Blast_Score=108, Evalue=1e-23,
Organism=Escherichia coli, GI1786946, Length=422, Percent_Identity=47.1563981042654, Blast_Score=389, Evalue=1e-109,
Organism=Escherichia coli, GI1786305, Length=432, Percent_Identity=33.3333333333333, Blast_Score=193, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI25146366, Length=423, Percent_Identity=41.371158392435, Blast_Score=311, Evalue=3e-85,
Organism=Caenorhabditis elegans, GI17537937, Length=442, Percent_Identity=26.4705882352941, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17560088, Length=453, Percent_Identity=29.3598233995585, Blast_Score=153, Evalue=1e-37,
Organism=Caenorhabditis elegans, GI17538894, Length=315, Percent_Identity=28.5714285714286, Blast_Score=120, Evalue=2e-27,
Organism=Saccharomyces cerevisiae, GI6320352, Length=427, Percent_Identity=43.0913348946136, Blast_Score=339, Evalue=4e-94,
Organism=Saccharomyces cerevisiae, GI6324258, Length=464, Percent_Identity=27.801724137931, Blast_Score=135, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24645909, Length=249, Percent_Identity=54.6184738955823, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI18859875, Length=425, Percent_Identity=30.3529411764706, Blast_Score=164, Evalue=1e-40,
Organism=Drosophila melanogaster, GI20129315, Length=233, Percent_Identity=30.9012875536481, Blast_Score=118, Evalue=8e-27,
Organism=Drosophila melanogaster, GI24582497, Length=233, Percent_Identity=30.4721030042918, Blast_Score=117, Evalue=2e-26,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 45176; Mature: 45045

Theoretical pI: Translated: 5.83; Mature: 5.83

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEIRVPTLGESVTEATIGKWFKKQGDAVSVDEPLVELETDKVTIEVPAPSAGTLAELVA
CCCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCEEEECCEEEEEECCCCCCHHHHHHH
RQGETVAVGALLGQITEGGAPAKPAAAKTQGISAESTTGRPDLKSDTTTPINAGPEEPRP
CCCCEEEHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RAEADAPLAPSVRKLSAESGIDASTVSGSGKDGRVTKGDMLAAIEKAASAPTPVDQPAAA
CCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCE
VQVRAPSPADDASREERVKMTRLRQTIARRLKDVQNTAAILTTFNEVDMSGVMALRAHYK
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
DVFEKKHGVKLGFMGFFTKAIVQALRDIPAVNAEIDGSDLIYKNYYHIGIAVGTDKGLVV
HHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEECCCCEEE
PVVRDCDQKSIAEIEKSVADYGRRARDGQLKIEEMQGGTFTITNGGIYGSLMSTPILNAP
HHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEECCCEEEHHHHCCCCCCC
QAGILGMHKIQERPVAIGGKVEIRPMMYLALSYDHRVIDGKEAVTFLVRVKESLEDPARL
CCCCCCHHHHHCCCEEECCEEEECEEEEEEEECCCEEECCHHHHHHHHHHHHHHCCCHHH
VLDL
EECC
>Mature Secondary Structure 
TEIRVPTLGESVTEATIGKWFKKQGDAVSVDEPLVELETDKVTIEVPAPSAGTLAELVA
CCCCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCEEEECCEEEEEECCCCCCHHHHHHH
RQGETVAVGALLGQITEGGAPAKPAAAKTQGISAESTTGRPDLKSDTTTPINAGPEEPRP
CCCCEEEHHHHHHHHHCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RAEADAPLAPSVRKLSAESGIDASTVSGSGKDGRVTKGDMLAAIEKAASAPTPVDQPAAA
CCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCE
VQVRAPSPADDASREERVKMTRLRQTIARRLKDVQNTAAILTTFNEVDMSGVMALRAHYK
EEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHH
DVFEKKHGVKLGFMGFFTKAIVQALRDIPAVNAEIDGSDLIYKNYYHIGIAVGTDKGLVV
HHHHHHCCCEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEEEECCCCEEE
PVVRDCDQKSIAEIEKSVADYGRRARDGQLKIEEMQGGTFTITNGGIYGSLMSTPILNAP
HHHHCCCHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEEEEECCCEEEHHHHCCCCCCC
QAGILGMHKIQERPVAIGGKVEIRPMMYLALSYDHRVIDGKEAVTFLVRVKESLEDPARL
CCCCCCHHHHHCCCEEECCEEEECEEEEEEEECCCEEECCHHHHHHHHHHHHHHCCCHHH
VLDL
EECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12874367 [H]