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The map label for this gene is fixJ [H]

Identifier: 75674440

GI number: 75674440

Start: 269923

End: 270573

Strand: Direct

Name: fixJ [H]

Synonym: Nwi_0241

Alternate gene names: 75674440

Gene position: 269923-270573 (Clockwise)

Preceding gene: 75674439

Following gene: 75674444

Centisome position: 7.93

GC content: 60.22

Gene sequence:

>651_bases
ATGGGAAAACTTCTGTCCTTTGTTTCGGATGTCTCGATTTTCATCCTCGACGATGATCCCTCGGTCCGTGAAACGCTGGT
CCTCCTCTTGAAGATCGAGGGTTATGCGGCGCAAGGCTTCGGCGACGGTTCATCGTTCCTTGAAGCGGTTCGCAACGCGT
CTCCTGCCTGTGTGATTCTCGACCTCAATCTTCCCGGAGAATCTGGATTTGATGTCCTGCGCAGGCTCGCCGACGAACGG
GTGGCCGCGCCGATCCTCGTGATCTCGGGCGCCGCCGACATTTCAATGGCCGTCGCGGCTATGAAGTACGGCGCGCTCGA
TTTCCTGGAGAAGCCGTTTTCCTCCGATGTCATCATTGAACGGGTGCGTGCCGCGGTGAAGGTATGGAGAGAAGCCCGAG
GGAGCGAGAGGGACGGCTTCGCCAACTTTCCCGGCCGCCATCTGCTGACCTGCCGTGAGCGCGAGGTTCTGTCTCAAGTC
GCCAGGGGCGCCTCGAACAAGGAGGCAGGGAGAAGACTTGGCATCAGCCCGCGTACCGTCGAGGTGCACCGCGCGCGAAT
CATGGACAAGCTCGGGGCCAAGAATACGGCCGATCTGATTCGGATCGTGTTCTCCGAGGGGAGCGCATGGCGGGGGCTTT
CCGCTTCATAG

Upstream 100 bases:

>100_bases
TCCAACTAGCATCGTGATCAGTAGCGTTTTCGAGCGAAGTGGAATCCGGTTCGCGTGAAGAAAACGCGTCAAAACTAAAG
ACTCTTTAGTGAGGAAGCGG

Downstream 100 bases:

>100_bases
AGATTCCTCCCGCTTCTGATGGAATCAGAAGCGAGGCCCTTCGTTCGGCTTCTCGCGGTTTCATCAAGCGATGGGCCGCC
GAGCAGACGGACAGGGACCC

Product: LuxR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MGKLLSFVSDVSIFILDDDPSVRETLVLLLKIEGYAAQGFGDGSSFLEAVRNASPACVILDLNLPGESGFDVLRRLADER
VAAPILVISGAADISMAVAAMKYGALDFLEKPFSSDVIIERVRAAVKVWREARGSERDGFANFPGRHLLTCREREVLSQV
ARGASNKEAGRRLGISPRTVEVHRARIMDKLGAKNTADLIRIVFSEGSAWRGLSAS

Sequences:

>Translated_216_residues
MGKLLSFVSDVSIFILDDDPSVRETLVLLLKIEGYAAQGFGDGSSFLEAVRNASPACVILDLNLPGESGFDVLRRLADER
VAAPILVISGAADISMAVAAMKYGALDFLEKPFSSDVIIERVRAAVKVWREARGSERDGFANFPGRHLLTCREREVLSQV
ARGASNKEAGRRLGISPRTVEVHRARIMDKLGAKNTADLIRIVFSEGSAWRGLSAS
>Mature_215_residues
GKLLSFVSDVSIFILDDDPSVRETLVLLLKIEGYAAQGFGDGSSFLEAVRNASPACVILDLNLPGESGFDVLRRLADERV
AAPILVISGAADISMAVAAMKYGALDFLEKPFSSDVIIERVRAAVKVWREARGSERDGFANFPGRHLLTCREREVLSQVA
RGASNKEAGRRLGISPRTVEVHRARIMDKLGAKNTADLIRIVFSEGSAWRGLSAS

Specific function: FixJ, when activated by fixL, induces the expression of both nifA, required for activation of classical nif and fix genes, and fixK, required for fixN activation [H]

COG id: COG4566

COG function: function code T; Response regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1788521, Length=199, Percent_Identity=31.1557788944724, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1790299, Length=121, Percent_Identity=28.9256198347107, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1790102, Length=197, Percent_Identity=30.4568527918782, Blast_Score=72, Evalue=2e-14,
Organism=Escherichia coli, GI1790860, Length=116, Percent_Identity=31.0344827586207, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI1788550, Length=141, Percent_Identity=24.822695035461, Blast_Score=68, Evalue=5e-13,
Organism=Escherichia coli, GI1786747, Length=208, Percent_Identity=25.4807692307692, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1790552, Length=117, Percent_Identity=28.2051282051282, Blast_Score=66, Evalue=2e-12,
Organism=Escherichia coli, GI1788712, Length=202, Percent_Identity=25.2475247524752, Blast_Score=63, Evalue=1e-11,
Organism=Escherichia coli, GI1789809, Length=119, Percent_Identity=26.0504201680672, Blast_Score=62, Evalue=2e-11,
Organism=Escherichia coli, GI1787229, Length=137, Percent_Identity=27.7372262773723, Blast_Score=62, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 23403; Mature: 23272

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKLLSFVSDVSIFILDDDPSVRETLVLLLKIEGYAAQGFGDGSSFLEAVRNASPACVIL
CCHHHHHHCCCEEEEECCCCCHHHHHEEHHEECCEEECCCCCHHHHHHHHHCCCCEEEEE
DLNLPGESGFDVLRRLADERVAAPILVISGAADISMAVAAMKYGALDFLEKPFSSDVIIE
EECCCCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
RVRAAVKVWREARGSERDGFANFPGRHLLTCREREVLSQVARGASNKEAGRRLGISPRTV
HHHHHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCHHCCCCCCCCCHH
EVHRARIMDKLGAKNTADLIRIVFSEGSAWRGLSAS
HHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
GKLLSFVSDVSIFILDDDPSVRETLVLLLKIEGYAAQGFGDGSSFLEAVRNASPACVIL
CHHHHHHCCCEEEEECCCCCHHHHHEEHHEECCEEECCCCCHHHHHHHHHCCCCEEEEE
DLNLPGESGFDVLRRLADERVAAPILVISGAADISMAVAAMKYGALDFLEKPFSSDVIIE
EECCCCCCCHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHHH
RVRAAVKVWREARGSERDGFANFPGRHLLTCREREVLSQVARGASNKEAGRRLGISPRTV
HHHHHHHHHHHHCCCCCCCCCCCCCCCEEHHHHHHHHHHHHCCCCCCHHCCCCCCCCCHH
EVHRARIMDKLGAKNTADLIRIVFSEGSAWRGLSAS
HHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2842062; 11481432; 2663474; 1857213; 10647182 [H]