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The map label for this gene is purC1 [H]

Identifier: 162139848

GI number: 162139848

Start: 1437734

End: 1438501

Strand: Direct

Name: purC1 [H]

Synonym: Nwi_1304

Alternate gene names: 162139848

Gene position: 1437734-1438501 (Clockwise)

Preceding gene: 75675494

Following gene: 75675497

Centisome position: 42.26

GC content: 60.16

Gene sequence:

>768_bases
ATGAGCCGTCGGCGTCGCATATACGAAGGCAAGGCCAAGGTGCTTTACGAAGGACCGGAGCCGGGCACCCTGATTCAACA
CTTCAAGGACGACGCGACCGCGTTCAATGCCAAGAAGCATCAGGTGATCGAGGGCAAGGGTGTCCTCAACAACAGGATTT
CGGAGTACCTGTTCCAGCACCTCAACGACATCGGGGTGCCGACGCATTTCATCCGTCGTCTCAACATGCGCGAGCAGTTG
ATCCGCGAGGTCGAGATCGTGCCGCTGGAAGTCGTGGTCCGCAACGTCGCGGCGGGGTCGCTGTCGCAGCGACTCGGCAT
CGAGGAGGGCACCCAGCTTCCGCGCTCCATCATCGAATTCTACTACAAGAACGACCAGCTCAACGACCCCATGGTGTCGG
AAGAGCACGTCACGGCGTTCGGCTGGGCGACGCCGCAGGAAATCGACGACATCATGGCGCTGGCGATCCGCGTCAACGAC
TTCCTGACCGGGCTGTTTCTCGGCATCGGCATCCGGCTTGTCGATTTCAAGATGGAATGCGGCCGGCTGTTCGAGAATGA
GATGATGCGAATCGTCGTCGCCGATGAAATATCGCCGGACTCGTGCCGGCTGTGGGACATCAAGTCGAACGAGAAGCTCG
ACAAGGATGTTTTCCGTCGCGATCTCGGCGGGCTTCTGGAAGCCTACACCGAAGTTGCCAAGCGGCTCGGCATCCTCATG
GAGAACGAGCGGCCGATCGGCGCCGGCCCGGTGCTGGTGAAGGGATAA

Upstream 100 bases:

>100_bases
GGTCCGGAACTCCTGGCCTTCGCGGCAGCTCCGCCACGGTCACATCAAGCGCTCGGAGCACACCAACTTCATGGATTTCA
ACAACACACGGTACATCCCA

Downstream 100 bases:

>100_bases
TCGTCCCTCTCCCTTGCGGGAGAGGGTGACGGGCAGCACAGGCAGGTTACGTTTCAAGATGAAGGCACGCGTCACTGTTA
CGTTGAAAGCCGGCGTTCTC

Product: phosphoribosylaminoimidazole-succinocarboxamide synthase

Products: NA

Alternate protein names: SAICAR synthetase A [H]

Number of amino acids: Translated: 255; Mature: 254

Protein sequence:

>255_residues
MSRRRRIYEGKAKVLYEGPEPGTLIQHFKDDATAFNAKKHQVIEGKGVLNNRISEYLFQHLNDIGVPTHFIRRLNMREQL
IREVEIVPLEVVVRNVAAGSLSQRLGIEEGTQLPRSIIEFYYKNDQLNDPMVSEEHVTAFGWATPQEIDDIMALAIRVND
FLTGLFLGIGIRLVDFKMECGRLFENEMMRIVVADEISPDSCRLWDIKSNEKLDKDVFRRDLGGLLEAYTEVAKRLGILM
ENERPIGAGPVLVKG

Sequences:

>Translated_255_residues
MSRRRRIYEGKAKVLYEGPEPGTLIQHFKDDATAFNAKKHQVIEGKGVLNNRISEYLFQHLNDIGVPTHFIRRLNMREQL
IREVEIVPLEVVVRNVAAGSLSQRLGIEEGTQLPRSIIEFYYKNDQLNDPMVSEEHVTAFGWATPQEIDDIMALAIRVND
FLTGLFLGIGIRLVDFKMECGRLFENEMMRIVVADEISPDSCRLWDIKSNEKLDKDVFRRDLGGLLEAYTEVAKRLGILM
ENERPIGAGPVLVKG
>Mature_254_residues
SRRRRIYEGKAKVLYEGPEPGTLIQHFKDDATAFNAKKHQVIEGKGVLNNRISEYLFQHLNDIGVPTHFIRRLNMREQLI
REVEIVPLEVVVRNVAAGSLSQRLGIEEGTQLPRSIIEFYYKNDQLNDPMVSEEHVTAFGWATPQEIDDIMALAIRVNDF
LTGLFLGIGIRLVDFKMECGRLFENEMMRIVVADEISPDSCRLWDIKSNEKLDKDVFRRDLGGLLEAYTEVAKRLGILME
NERPIGAGPVLVKG

Specific function: De novo purine biosynthesis; seventh step. [C]

COG id: COG0152

COG function: function code F; Phosphoribosylaminoimidazolesuccinocarboxamide (SAICAR) synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the SAICAR synthetase family [H]

Homologues:

Organism=Homo sapiens, GI119220557, Length=256, Percent_Identity=32.8125, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI5453539, Length=256, Percent_Identity=32.8125, Blast_Score=114, Evalue=1e-25,
Organism=Homo sapiens, GI119220559, Length=263, Percent_Identity=31.5589353612167, Blast_Score=103, Evalue=1e-22,
Organism=Escherichia coli, GI1788820, Length=241, Percent_Identity=41.49377593361, Blast_Score=204, Evalue=6e-54,
Organism=Caenorhabditis elegans, GI17531275, Length=243, Percent_Identity=30.4526748971193, Blast_Score=94, Evalue=6e-20,
Organism=Drosophila melanogaster, GI18860083, Length=238, Percent_Identity=30.2521008403361, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24583917, Length=207, Percent_Identity=26.0869565217391, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: 1680 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 13,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013816
- InterPro:   IPR001636
- InterPro:   IPR018236 [H]

Pfam domain/function: PF01259 SAICAR_synt [H]

EC number: =6.3.2.6 [H]

Molecular weight: Translated: 29097; Mature: 28966

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS01057 SAICAR_SYNTHETASE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRRRIYEGKAKVLYEGPEPGTLIQHFKDDATAFNAKKHQVIEGKGVLNNRISEYLFQH
CCCCCCHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCHHEEECCCCHHHHHHHHHHHHH
LNDIGVPTHFIRRLNMREQLIREVEIVPLEVVVRNVAAGSLSQRLGIEEGTQLPRSIIEF
HHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
YYKNDQLNDPMVSEEHVTAFGWATPQEIDDIMALAIRVNDFLTGLFLGIGIRLVDFKMEC
HHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEEHHHHH
GRLFENEMMRIVVADEISPDSCRLWDIKSNEKLDKDVFRRDLGGLLEAYTEVAKRLGILM
HHHHHHHHEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEE
ENERPIGAGPVLVKG
CCCCCCCCCCEEECC
>Mature Secondary Structure 
SRRRRIYEGKAKVLYEGPEPGTLIQHFKDDATAFNAKKHQVIEGKGVLNNRISEYLFQH
CCCCCHHCCCCEEEEECCCCCHHHHHHHHHHHHHCCCHHEEECCCCHHHHHHHHHHHHH
LNDIGVPTHFIRRLNMREQLIREVEIVPLEVVVRNVAAGSLSQRLGIEEGTQLPRSIIEF
HHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
YYKNDQLNDPMVSEEHVTAFGWATPQEIDDIMALAIRVNDFLTGLFLGIGIRLVDFKMEC
HHCCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHCHHEEEHHHHH
GRLFENEMMRIVVADEISPDSCRLWDIKSNEKLDKDVFRRDLGGLLEAYTEVAKRLGILM
HHHHHHHHEEEEEECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCEE
ENERPIGAGPVLVKG
CCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12597275 [H]