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The map label for this gene is dsbA [H]

Identifier: 74318802

GI number: 74318802

Start: 2865634

End: 2866284

Strand: Direct

Name: dsbA [H]

Synonym: Tbd_2784

Alternate gene names: 74318802

Gene position: 2865634-2866284 (Clockwise)

Preceding gene: 74318801

Following gene: 74318803

Centisome position: 98.48

GC content: 64.36

Gene sequence:

>651_bases
ATGTTTGCCCGCGCACTGACGCTGTTTGTCGCCGCCGTTTTCTCCGTGTCGAGCCTTGCCGCGTCGGGGGACGCGTTCGA
AGGCCACGACTACGCGCGGGTGAAGAATCCGCAAGCGGTCGCGACCGGCAAGAAGGTCGAAGTCCTCGAGTTCTTCTGGT
ACCGCTGTCCGCACTGTTTCCAGCTCGAGCCGAGTCTCGCCAAATGGATCAAGGCCCTGCCGAAGGACGCCCAGATCCGC
CGCGTTCCCGCGGTGTTCCGCGAGGACTGGATGCCGGGCGCCAAGCTCTACTACACGCTCGAGCAGATGGGCCTGCTCGA
GCGCCTGCACGCCAAGGTCTTCGACGCCTACCACCTGCAGAACCTCGATCTCAACGACCCCGCCGTGCTCGGCAAGTGGA
TCGCCAAACAGGGCGTCGACCGCAAGAAGTTCGAGAGCACCTACAAATCCTTCTCGACCCAGTCGAAGGCGACCCAGGGC
GCGCGCCTCGCGATGACCTACGGCATCACCGGCGTGCCGGCCTTCATCGTCGACGGCAAATACATGACGTCGGTCGGCAT
GACCGGCAGCGAGGCGCGGCTGTTCGAAGTACTCGACGAACTGATCGCCAAGGCACGGGCCGAGCGGGCCGGTAAGAAAG
CCGCGCCCTAA

Upstream 100 bases:

>100_bases
ACGCGCCGGCAGACGCCGAGCGGACGCGCGCCCTGCTCGCGGAGAACGATATTTCCGCGACGCTGGTCAAAGAACTTCCC
ACTCAACAGGAGACGCCTTG

Downstream 100 bases:

>100_bases
GCCCTTGCCCGCCGCGCCGCTCAAGGTTTTCATCACCGGCGCGAGCTCCGGTCTCGGCGCGGCGCTCGCCCGCCATTACG
GCGCACGCGGTGCGCAACTC

Product: thiol:disulfide interchange protein DsbA

Products: 3-oxo-D 4-steroid; protein with oxidized disulfide bonds [C]

Alternate protein names: NA

Number of amino acids: Translated: 216; Mature: 216

Protein sequence:

>216_residues
MFARALTLFVAAVFSVSSLAASGDAFEGHDYARVKNPQAVATGKKVEVLEFFWYRCPHCFQLEPSLAKWIKALPKDAQIR
RVPAVFREDWMPGAKLYYTLEQMGLLERLHAKVFDAYHLQNLDLNDPAVLGKWIAKQGVDRKKFESTYKSFSTQSKATQG
ARLAMTYGITGVPAFIVDGKYMTSVGMTGSEARLFEVLDELIAKARAERAGKKAAP

Sequences:

>Translated_216_residues
MFARALTLFVAAVFSVSSLAASGDAFEGHDYARVKNPQAVATGKKVEVLEFFWYRCPHCFQLEPSLAKWIKALPKDAQIR
RVPAVFREDWMPGAKLYYTLEQMGLLERLHAKVFDAYHLQNLDLNDPAVLGKWIAKQGVDRKKFESTYKSFSTQSKATQG
ARLAMTYGITGVPAFIVDGKYMTSVGMTGSEARLFEVLDELIAKARAERAGKKAAP
>Mature_216_residues
MFARALTLFVAAVFSVSSLAASGDAFEGHDYARVKNPQAVATGKKVEVLEFFWYRCPHCFQLEPSLAKWIKALPKDAQIR
RVPAVFREDWMPGAKLYYTLEQMGLLERLHAKVFDAYHLQNLDLNDPAVLGKWIAKQGVDRKKFESTYKSFSTQSKATQG
ARLAMTYGITGVPAFIVDGKYMTSVGMTGSEARLFEVLDELIAKARAERAGKKAAP

Specific function: Involved in disulfide-bond formation. Acts by transferring its disulfide bond to other proteins. Involved in the production of protease and alkaline phosphatase, motility, metal resistance, and multi-drug resistance [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thioredoxin family. DsbA subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: 140 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1348 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001853
- InterPro:   IPR023205
- InterPro:   IPR017936
- InterPro:   IPR012336
- InterPro:   IPR017937
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01323 DSBA [H]

EC number: NA

Molecular weight: Translated: 24075; Mature: 24075

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFARALTLFVAAVFSVSSLAASGDAFEGHDYARVKNPQAVATGKKVEVLEFFWYRCPHCF
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCHHHCCCHHHHHHHHHHHCCCCE
QLEPSLAKWIKALPKDAQIRRVPAVFREDWMPGAKLYYTLEQMGLLERLHAKVFDAYHLQ
EECHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
NLDLNDPAVLGKWIAKQGVDRKKFESTYKSFSTQSKATQGARLAMTYGITGVPAFIVDGK
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEECCC
YMTSVGMTGSEARLFEVLDELIAKARAERAGKKAAP
CHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MFARALTLFVAAVFSVSSLAASGDAFEGHDYARVKNPQAVATGKKVEVLEFFWYRCPHCF
CHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCCHHHCCCHHHHHHHHHHHCCCCE
QLEPSLAKWIKALPKDAQIRRVPAVFREDWMPGAKLYYTLEQMGLLERLHAKVFDAYHLQ
EECHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
NLDLNDPAVLGKWIAKQGVDRKKFESTYKSFSTQSKATQGARLAMTYGITGVPAFIVDGK
CCCCCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEECCC
YMTSVGMTGSEARLFEVLDELIAKARAERAGKKAAP
CHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 3-oxo-D 5-steroid; protein with reduced SH groups [C]

Specific reaction: 3-oxo-D 5-steroid = 3-oxo-D 4-steroid protein with reduced SH groups = protein with oxidized disulfide bonds [C]

General reaction: Isomerases; Intramolecular Oxidoreductases; Transposing S-S Bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10711598 [H]