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The map label for this gene is priA [H]

Identifier: 74318799

GI number: 74318799

Start: 2861158

End: 2863317

Strand: Direct

Name: priA [H]

Synonym: Tbd_2781

Alternate gene names: 74318799

Gene position: 2861158-2863317 (Clockwise)

Preceding gene: 74318798

Following gene: 74318800

Centisome position: 98.33

GC content: 71.9

Gene sequence:

>2160_bases
ATGACTCCGATCGTCCAGGTTGGACTCGACACGCCGCTTGACCGTCTGTTCGATTACAGCGCGGGCGAAGCCGCGGCGGA
CCTCGCCCCCGGCCGCCTCGTCGAAGTACCCTTCGGCCGCACCCGGCAGGTCGGCGTGGTGCTCGGCAGCCTTGCTGAGA
CGGCCGTCGACGGGATCAAGCTGAGGACAATCCTTCGCGTCCTCGACGACCGCCCCGCGCTCGCGCCGGAAGTGCTGCGC
TTGGCACGGTTCTGCGCCGACTACTACCACTATCCGCTGGGGGCGGTCCTGCTCGCGACCCTTCCCCCCCGCCTGAAGAA
CGCGACCCCCTACCTCGCCGACACGCCGTGGCTCGTCGTCACCGCGCTTGGCCGCGCCGAGGGACCCGCGCCGAGGGCCC
GCGCGCAGCATGCCCTGCTCGAAGCCTTGCGCGAGGCGCCGCAGGCGCGTGCATACCTGCGCGCCATCAAGCAGGGACGC
CACGCGGCCGCGCTCGTCGCCGCGGGCTGGGCCGAATGGACCCGCCTGCCGCCCGAGGCCGAACCCCCGCCGGAAGGCCC
TCGCCCGGCACCGGCAACGGCCGAGCAGCAGGCCGCGCTCGATGTGTTGCTGCCGACGCTCGGCGAATTCGCCGTGCATC
TCGTGCACGGCGTGACCGGCAGCGGCAAGACCGAACTCTATCTGCGCGTCATCGACGCCGTGCTCGCCGCCGGTCGGCAG
GCGCTGGTACTGATTCCGGAAATCGCGCTGACGCCCCAGCTCGAAGGACACTTCCGCGCCCGCTTTCCGGGCCGGCGCTT
CGCGACCCTGCACAGCGGGCTTGCCGAGGCCGCGCGCACCGAAAACTGGCTCGCCGCAGCCGACTGCGACGTCCTGCTCG
GCACCCGCCTCGCCGTATTCGCGCCGCTGCCGCGGCTGGGCCTGATCGTCGTCGACGAAGAACACGACACCTCATTCAAG
CAGCAGGACGGCTTGCGCTATTCGGCGCGCGACGTCGCGATCGCCCGCGGCAAGCAGGCCGGCGTTCCGGTGCTGCTCGG
GTCGGCCACGCCATCGCTCGAAAGCTACGCGCAGGCCCGCAGCGGCCGCTACCGGCTGCTCGAACTCAGGCAGCGCGCGA
TCGCCGCCGCGCGCCTGCCCGAGATCGAGCTCGTCGACCTGCGTCACGTTCCGCAGGAAAACGGCCTGACCGCGCCGGCC
CTCGCGGCCCTGCACGCGACGCTCGCACGCGGCGAGCAGAGCCTGGTCTTCCTCAATCGCCGCGGCTATGCGCCCGCGCT
GTACTGTCCGAGCTGCGCCTGGGTCTCGCCCTGCCCGCACTGCTCGGCACGGCTGGTCCTGCACCGCGGCGCGCAGCGGC
TGAAATGCCATCACTGCGGCTTCGAGACACGGATCCCGAGCGCATGCCCGGACTGCGGCGAGCCCGACCTGAAGCCGCTC
GGGCAGGGCACTCAGCGCGTCGAGGAAACGCTCGCGACGCACCTGCCGGGTGCGCGCATCCGCCGCATCGACCGCGACAC
GATGCGCCCGCGCGCCTGGGCCGAGCTCGGCACGGCCGTGCACGGCGGCGAAGTCGACGTGCTCGTCGGCACGCAGATGC
TGGCCAAGGGCCACGACTTCCCGAACGTGACGCTGGCGCTGATACTCGACACCGACGGCGCGCTCTACAGCCCCGACTTT
CGGGCGACGGAACGGCTTTTCGCCCAACTCATGCAGGTCGCCGGGCGCGCGGGCCGCGCGGAAAAACCCGGCCGCGTGCT
GATCCAGACGGCGTTTCCCGACCACCCGCTGTTCCGTCACCTGCAGCGACACGACTACGCGGCCTACGCACGCGAACTGC
TCGCCGAACGCAAACAGCTCGAACTGCCCCCGTACACGCGGCAGATCCTCGTGCGCGCCGAAGCGACCACGATGGACGCC
GCGCTCGCCTTCCTGCGGCGCGCGGCGGCGCTCGCCCCCGATGCGCCCGGGGTCAGCGTGTTCGCTGCGACGCCCGCGCC
GATGGCCCGCGTTGCCAAGCTCGAACGCGCGCAACTTCTGATCCAGTCGCCCTCACGCGCGGCGCTGCAGGCCTTCGTGC
GGGACTGGCGGCCGCGTCTCGAAACGATCCGGCCGCGCCCCGCGCGCTGGTCGCTCGACGTCGACCCGCTCGTGTTTTGA

Upstream 100 bases:

>100_bases
CCCCCAGTGTCAACCAAGGACCGGCATGAATGACCCCCAAACCGGGCAAACGCGTCGACCTTGCGTCCCGAGCCCCGACT
CTCAGCAAGGGGCGGACTGA

Downstream 100 bases:

>100_bases
GTCCGGCGCGGCGCGCGGTTTTGAGGCCTCGCGGGCGGCCCTTATAATCGCCGGCTTGCCATCCGAACGCCGAATTCGAC
GAGTTCAGGGCCCACACGCC

Product: replication restart DNA helicase PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 719; Mature: 718

Protein sequence:

>719_residues
MTPIVQVGLDTPLDRLFDYSAGEAAADLAPGRLVEVPFGRTRQVGVVLGSLAETAVDGIKLRTILRVLDDRPALAPEVLR
LARFCADYYHYPLGAVLLATLPPRLKNATPYLADTPWLVVTALGRAEGPAPRARAQHALLEALREAPQARAYLRAIKQGR
HAAALVAAGWAEWTRLPPEAEPPPEGPRPAPATAEQQAALDVLLPTLGEFAVHLVHGVTGSGKTELYLRVIDAVLAAGRQ
ALVLIPEIALTPQLEGHFRARFPGRRFATLHSGLAEAARTENWLAAADCDVLLGTRLAVFAPLPRLGLIVVDEEHDTSFK
QQDGLRYSARDVAIARGKQAGVPVLLGSATPSLESYAQARSGRYRLLELRQRAIAAARLPEIELVDLRHVPQENGLTAPA
LAALHATLARGEQSLVFLNRRGYAPALYCPSCAWVSPCPHCSARLVLHRGAQRLKCHHCGFETRIPSACPDCGEPDLKPL
GQGTQRVEETLATHLPGARIRRIDRDTMRPRAWAELGTAVHGGEVDVLVGTQMLAKGHDFPNVTLALILDTDGALYSPDF
RATERLFAQLMQVAGRAGRAEKPGRVLIQTAFPDHPLFRHLQRHDYAAYARELLAERKQLELPPYTRQILVRAEATTMDA
ALAFLRRAAALAPDAPGVSVFAATPAPMARVAKLERAQLLIQSPSRAALQAFVRDWRPRLETIRPRPARWSLDVDPLVF

Sequences:

>Translated_719_residues
MTPIVQVGLDTPLDRLFDYSAGEAAADLAPGRLVEVPFGRTRQVGVVLGSLAETAVDGIKLRTILRVLDDRPALAPEVLR
LARFCADYYHYPLGAVLLATLPPRLKNATPYLADTPWLVVTALGRAEGPAPRARAQHALLEALREAPQARAYLRAIKQGR
HAAALVAAGWAEWTRLPPEAEPPPEGPRPAPATAEQQAALDVLLPTLGEFAVHLVHGVTGSGKTELYLRVIDAVLAAGRQ
ALVLIPEIALTPQLEGHFRARFPGRRFATLHSGLAEAARTENWLAAADCDVLLGTRLAVFAPLPRLGLIVVDEEHDTSFK
QQDGLRYSARDVAIARGKQAGVPVLLGSATPSLESYAQARSGRYRLLELRQRAIAAARLPEIELVDLRHVPQENGLTAPA
LAALHATLARGEQSLVFLNRRGYAPALYCPSCAWVSPCPHCSARLVLHRGAQRLKCHHCGFETRIPSACPDCGEPDLKPL
GQGTQRVEETLATHLPGARIRRIDRDTMRPRAWAELGTAVHGGEVDVLVGTQMLAKGHDFPNVTLALILDTDGALYSPDF
RATERLFAQLMQVAGRAGRAEKPGRVLIQTAFPDHPLFRHLQRHDYAAYARELLAERKQLELPPYTRQILVRAEATTMDA
ALAFLRRAAALAPDAPGVSVFAATPAPMARVAKLERAQLLIQSPSRAALQAFVRDWRPRLETIRPRPARWSLDVDPLVF
>Mature_718_residues
TPIVQVGLDTPLDRLFDYSAGEAAADLAPGRLVEVPFGRTRQVGVVLGSLAETAVDGIKLRTILRVLDDRPALAPEVLRL
ARFCADYYHYPLGAVLLATLPPRLKNATPYLADTPWLVVTALGRAEGPAPRARAQHALLEALREAPQARAYLRAIKQGRH
AAALVAAGWAEWTRLPPEAEPPPEGPRPAPATAEQQAALDVLLPTLGEFAVHLVHGVTGSGKTELYLRVIDAVLAAGRQA
LVLIPEIALTPQLEGHFRARFPGRRFATLHSGLAEAARTENWLAAADCDVLLGTRLAVFAPLPRLGLIVVDEEHDTSFKQ
QDGLRYSARDVAIARGKQAGVPVLLGSATPSLESYAQARSGRYRLLELRQRAIAAARLPEIELVDLRHVPQENGLTAPAL
AALHATLARGEQSLVFLNRRGYAPALYCPSCAWVSPCPHCSARLVLHRGAQRLKCHHCGFETRIPSACPDCGEPDLKPLG
QGTQRVEETLATHLPGARIRRIDRDTMRPRAWAELGTAVHGGEVDVLVGTQMLAKGHDFPNVTLALILDTDGALYSPDFR
ATERLFAQLMQVAGRAGRAEKPGRVLIQTAFPDHPLFRHLQRHDYAAYARELLAERKQLELPPYTRQILVRAEATTMDAA
LAFLRRAAALAPDAPGVSVFAATPAPMARVAKLERAQLLIQSPSRAALQAFVRDWRPRLETIRPRPARWSLDVDPLVF

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=744, Percent_Identity=39.9193548387097, Blast_Score=484, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 78364; Mature: 78233

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPIVQVGLDTPLDRLFDYSAGEAAADLAPGRLVEVPFGRTRQVGVVLGSLAETAVDGIK
CCCEEEECCCCCHHHHHCCCCCCHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHH
LRTILRVLDDRPALAPEVLRLARFCADYYHYPLGAVLLATLPPRLKNATPYLADTPWLVV
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCEEE
TALGRAEGPAPRARAQHALLEALREAPQARAYLRAIKQGRHAAALVAAGWAEWTRLPPEA
EECCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHCHHHHHCCCCCC
EPPPEGPRPAPATAEQQAALDVLLPTLGEFAVHLVHGVTGSGKTELYLRVIDAVLAAGRQ
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCC
ALVLIPEIALTPQLEGHFRARFPGRRFATLHSGLAEAARTENWLAAADCDVLLGTRLAVF
EEEEECCEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCHHHHHCHHHHH
APLPRLGLIVVDEEHDTSFKQQDGLRYSARDVAIARGKQAGVPVLLGSATPSLESYAQAR
CCCCCCCEEEEECCCCCCHHHHCCCCCCHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHH
SGRYRLLELRQRAIAAARLPEIELVDLRHVPQENGLTAPALAALHATLARGEQSLVFLNR
CCCHHHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEC
RGYAPALYCPSCAWVSPCPHCSARLVLHRGAQRLKCHHCGFETRIPSACPDCGEPDLKPL
CCCCCEEECCCCCCCCCCCCHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
GQGTQRVEETLATHLPGARIRRIDRDTMRPRAWAELGTAVHGGEVDVLVGTQMLAKGHDF
CCHHHHHHHHHHHHCCCHHHHHCCHHHCCCHHHHHHCCCCCCCCEEEEECHHHHHCCCCC
PNVTLALILDTDGALYSPDFRATERLFAQLMQVAGRAGRAEKPGRVLIQTAFPDHPLFRH
CCEEEEEEEECCCCEECCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHH
LQRHDYAAYARELLAERKQLELPPYTRQILVRAEATTMDAALAFLRRAAALAPDAPGVSV
HHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCEE
FAATPAPMARVAKLERAQLLIQSPSRAALQAFVRDWRPRLETIRPRPARWSLDVDPLVF
EEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TPIVQVGLDTPLDRLFDYSAGEAAADLAPGRLVEVPFGRTRQVGVVLGSLAETAVDGIK
CCEEEECCCCCHHHHHCCCCCCHHHHCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHH
LRTILRVLDDRPALAPEVLRLARFCADYYHYPLGAVLLATLPPRLKNATPYLADTPWLVV
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCEEE
TALGRAEGPAPRARAQHALLEALREAPQARAYLRAIKQGRHAAALVAAGWAEWTRLPPEA
EECCCCCCCCCHHHHHHHHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHCHHHHHCCCCCC
EPPPEGPRPAPATAEQQAALDVLLPTLGEFAVHLVHGVTGSGKTELYLRVIDAVLAAGRQ
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCC
ALVLIPEIALTPQLEGHFRARFPGRRFATLHSGLAEAARTENWLAAADCDVLLGTRLAVF
EEEEECCEEECCCCCCCEEECCCCCHHHHHHHHHHHHHHCCCCEEECCCHHHHHCHHHHH
APLPRLGLIVVDEEHDTSFKQQDGLRYSARDVAIARGKQAGVPVLLGSATPSLESYAQAR
CCCCCCCEEEEECCCCCCHHHHCCCCCCHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHH
SGRYRLLELRQRAIAAARLPEIELVDLRHVPQENGLTAPALAALHATLARGEQSLVFLNR
CCCHHHHHHHHHHHHHHCCCCEEEEHHHCCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEC
RGYAPALYCPSCAWVSPCPHCSARLVLHRGAQRLKCHHCGFETRIPSACPDCGEPDLKPL
CCCCCEEECCCCCCCCCCCCHHHHHHHHHCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
GQGTQRVEETLATHLPGARIRRIDRDTMRPRAWAELGTAVHGGEVDVLVGTQMLAKGHDF
CCHHHHHHHHHHHHCCCHHHHHCCHHHCCCHHHHHHCCCCCCCCEEEEECHHHHHCCCCC
PNVTLALILDTDGALYSPDFRATERLFAQLMQVAGRAGRAEKPGRVLIQTAFPDHPLFRH
CCEEEEEEEECCCCEECCCHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCHHHHH
LQRHDYAAYARELLAERKQLELPPYTRQILVRAEATTMDAALAFLRRAAALAPDAPGVSV
HHHHHHHHHHHHHHHHHHHCCCCCHHHHEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCEE
FAATPAPMARVAKLERAQLLIQSPSRAALQAFVRDWRPRLETIRPRPARWSLDVDPLVF
EEECCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2162050; 2162049; 8346018; 9278503; 1662369 [H]