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The map label for this gene is rfbB [C]

Identifier: 74318717

GI number: 74318717

Start: 2781180

End: 2782190

Strand: Direct

Name: rfbB [C]

Synonym: Tbd_2699

Alternate gene names: 74318717

Gene position: 2781180-2782190 (Clockwise)

Preceding gene: 74318715

Following gene: 74318718

Centisome position: 95.58

GC content: 61.52

Gene sequence:

>1011_bases
ATGAAAATATTGGTTACTGGAGCCGCCGGTTTTATCGGCATGCACGTCGCGCAAATCCTCTTGCAGCGCGGCGACGAAGT
CGTCGGCATCGACAGCCTCAACGACTACTACGACCCCGCCTTGAAGCTCGCGCGGCTCGAACAGTTGAAGCCGCACCCGA
ACTTCCGCTTCGTCAGGGACGACATCTCCGACCGCATGGTGATGGAGGACCTGTTCGAGAAAGGCCACTTCGACGCCGTC
ATCAACCTCGCCGCGCAGGCCGGCGTGCGCTACTCGCTGAAGAATCCGCACGCCTACGTGCAATCGAACCTGGTGGGCTT
CGCCAACCTGCTCGAAGGCTGCCGCCACCATGGCGTCAAGCACTTCGTCTACGCGAGTTCATCGAGCGTCTACGGCGCCA
ATACAAAAATTCCGTTCTCGACCCACGACCCGGTCAACCATCCGGTCAGCCTCTACGCTGCGAGCAAGAAAGCCAACGAG
CTGATGGCGCACACCTACTCGCATCTCTACGGCCTGCCGACCACGGGGCTGCGCTACTTCACGGTCTACGGCCCCTGGGG
CCGGCCCGACATGTCACCGTGGCTCTTCACCTCGGCGATTCTCGAAGGCCGCAGCATCGACGTCTTCAACCACGGCGACA
TGATGCGCGACTTCACCTACATCGACGACATCGCCGACGGCACGGTCAAGGTCCTCGACCGCATCCCGCAGCCCGACCCG
AACTTCGATCACGCCAACCCCGACCCCGCGTCGAGCCACGCGCCCTACCGCGTCTACAACATCGGCAACCACACTCCGGT
GCAGTTGATGGATTTCATCGGCACGATCGAGAAGGCGCTCGGGCAGGAAGCGCGGAAGAATTTCCTGCCGATGCAGGACG
GTGACGTGAAGATGACCTACGCCGACGTCGACGATCTGATTCGCGATACCGGATTCAAGCCGGCGACGACGCTGGAGTAC
GGGATCGGCAAGTGGGTCGAGTGGTACCGGGGGTATAAGAACAGGTCGTGA

Upstream 100 bases:

>100_bases
CTTGTCGCAGGTTCCGGCGGCTTCAGTACCGGCCGCTCACTTCGCTCACTCAGCTTGCGCAAAGCGCAGGTCAGCCTTCT
CTGAAACCGGCGGACTCAAC

Downstream 100 bases:

>100_bases
GGAGACTCTGGCGAGCTGCCGGGTACCGACCTGTCGGAATTTTTTCGGCGCAACGCCGCGAAGAAGCGGGCTCGACAGGA
AACGCTTCGCTCACGCTCCG

Product: nucleoside-diphosphate-sugar epimerase

Products: dTDP-4-dehydro-6-deoxy-D-glucose; H2O [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MKILVTGAAGFIGMHVAQILLQRGDEVVGIDSLNDYYDPALKLARLEQLKPHPNFRFVRDDISDRMVMEDLFEKGHFDAV
INLAAQAGVRYSLKNPHAYVQSNLVGFANLLEGCRHHGVKHFVYASSSSVYGANTKIPFSTHDPVNHPVSLYAASKKANE
LMAHTYSHLYGLPTTGLRYFTVYGPWGRPDMSPWLFTSAILEGRSIDVFNHGDMMRDFTYIDDIADGTVKVLDRIPQPDP
NFDHANPDPASSHAPYRVYNIGNHTPVQLMDFIGTIEKALGQEARKNFLPMQDGDVKMTYADVDDLIRDTGFKPATTLEY
GIGKWVEWYRGYKNRS

Sequences:

>Translated_336_residues
MKILVTGAAGFIGMHVAQILLQRGDEVVGIDSLNDYYDPALKLARLEQLKPHPNFRFVRDDISDRMVMEDLFEKGHFDAV
INLAAQAGVRYSLKNPHAYVQSNLVGFANLLEGCRHHGVKHFVYASSSSVYGANTKIPFSTHDPVNHPVSLYAASKKANE
LMAHTYSHLYGLPTTGLRYFTVYGPWGRPDMSPWLFTSAILEGRSIDVFNHGDMMRDFTYIDDIADGTVKVLDRIPQPDP
NFDHANPDPASSHAPYRVYNIGNHTPVQLMDFIGTIEKALGQEARKNFLPMQDGDVKMTYADVDDLIRDTGFKPATTLEY
GIGKWVEWYRGYKNRS
>Mature_336_residues
MKILVTGAAGFIGMHVAQILLQRGDEVVGIDSLNDYYDPALKLARLEQLKPHPNFRFVRDDISDRMVMEDLFEKGHFDAV
INLAAQAGVRYSLKNPHAYVQSNLVGFANLLEGCRHHGVKHFVYASSSSVYGANTKIPFSTHDPVNHPVSLYAASKKANE
LMAHTYSHLYGLPTTGLRYFTVYGPWGRPDMSPWLFTSAILEGRSIDVFNHGDMMRDFTYIDDIADGTVKVLDRIPQPDP
NFDHANPDPASSHAPYRVYNIGNHTPVQLMDFIGTIEKALGQEARKNFLPMQDGDVKMTYADVDDLIRDTGFKPATTLEY
GIGKWVEWYRGYKNRS

Specific function: DTDP-L-RHAMNOSE BIOSYNTHESIS WITHIN THE O ANTIGEN BIOSYNTHESIS PATHWAY OF LIPOPOLYSACCHARIDE BIOSYNTHESIS. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sugar epimerase family. dTDP-glucose dehydratase subfamily [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=341, Percent_Identity=27.5659824046921, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI56237023, Length=340, Percent_Identity=27.9411764705882, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI56118217, Length=340, Percent_Identity=27.9411764705882, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI189083684, Length=340, Percent_Identity=27.9411764705882, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI42516563, Length=339, Percent_Identity=24.4837758112094, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI193211614, Length=292, Percent_Identity=23.6301369863014, Blast_Score=69, Evalue=6e-12,
Organism=Homo sapiens, GI8393516, Length=292, Percent_Identity=23.6301369863014, Blast_Score=69, Evalue=6e-12,
Organism=Escherichia coli, GI1788353, Length=356, Percent_Identity=29.4943820224719, Blast_Score=129, Evalue=4e-31,
Organism=Escherichia coli, GI1786974, Length=338, Percent_Identity=26.9230769230769, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI48994969, Length=351, Percent_Identity=28.7749287749288, Blast_Score=120, Evalue=9e-29,
Organism=Escherichia coli, GI1788365, Length=351, Percent_Identity=19.9430199430199, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI1788366, Length=177, Percent_Identity=29.3785310734463, Blast_Score=62, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI71982035, Length=352, Percent_Identity=27.5568181818182, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71982038, Length=354, Percent_Identity=27.683615819209, Blast_Score=117, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI17568069, Length=341, Percent_Identity=25.2199413489736, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI115532424, Length=342, Percent_Identity=25.1461988304094, Blast_Score=86, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17539532, Length=344, Percent_Identity=24.4186046511628, Blast_Score=85, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6319493, Length=353, Percent_Identity=26.628895184136, Blast_Score=92, Evalue=1e-19,
Organism=Drosophila melanogaster, GI19923002, Length=347, Percent_Identity=27.9538904899135, Blast_Score=125, Evalue=4e-29,
Organism=Drosophila melanogaster, GI21356223, Length=345, Percent_Identity=23.768115942029, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040
- InterPro:   IPR008089 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: 4.2.1.46 [C]

Molecular weight: Translated: 37803; Mature: 37803

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVTGAAGFIGMHVAQILLQRGDEVVGIDSLNDYYDPALKLARLEQLKPHPNFRFVRD
CEEEEECCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEHH
DISDRMVMEDLFEKGHFDAVINLAAQAGVRYSLKNPHAYVQSNLVGFANLLEGCRHHGVK
CHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCE
HFVYASSSSVYGANTKIPFSTHDPVNHPVSLYAASKKANELMAHTYSHLYGLPTTGLRYF
EEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEE
TVYGPWGRPDMSPWLFTSAILEGRSIDVFNHGDMMRDFTYIDDIADGTVKVLDRIPQPDP
EEECCCCCCCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHCCCCCC
NFDHANPDPASSHAPYRVYNIGNHTPVQLMDFIGTIEKALGQEARKNFLPMQDGDVKMTY
CCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
ADVDDLIRDTGFKPATTLEYGIGKWVEWYRGYKNRS
CCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKILVTGAAGFIGMHVAQILLQRGDEVVGIDSLNDYYDPALKLARLEQLKPHPNFRFVRD
CEEEEECCHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHHCCCCCCCEEEHH
DISDRMVMEDLFEKGHFDAVINLAAQAGVRYSLKNPHAYVQSNLVGFANLLEGCRHHGVK
CHHHHHHHHHHHHCCCHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCE
HFVYASSSSVYGANTKIPFSTHDPVNHPVSLYAASKKANELMAHTYSHLYGLPTTGLRYF
EEEEECCCCCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEE
TVYGPWGRPDMSPWLFTSAILEGRSIDVFNHGDMMRDFTYIDDIADGTVKVLDRIPQPDP
EEECCCCCCCCCHHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCHHHHHHHHCCCCCC
NFDHANPDPASSHAPYRVYNIGNHTPVQLMDFIGTIEKALGQEARKNFLPMQDGDVKMTY
CCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
ADVDDLIRDTGFKPATTLEYGIGKWVEWYRGYKNRS
CCHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NAD+ [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.019 {NAD+}} 0.034 {dTDPglucose}} [C]

Substrates: dTDPglucose [C]

Specific reaction: dTDPglucose --> dTDP-4-dehydro-6-deoxy-D-glucose + H2O [C]

General reaction: Elimination (of H2O C-O bond cleavage [C]

Inhibitor: TDP; TTP [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7682279 [H]