Click here to switch to the map view.

The map label for this gene is ascD [H]

Identifier: 74318581

GI number: 74318581

Start: 2637542

End: 2638579

Strand: Direct

Name: ascD [H]

Synonym: Tbd_2563

Alternate gene names: 74318581

Gene position: 2637542-2638579 (Clockwise)

Preceding gene: 74318579

Following gene: 74318587

Centisome position: 90.64

GC content: 64.64

Gene sequence:

>1038_bases
ATGTCGCATCAAGTCACGATTCAGCCCAGCGGACACCGGTTCACCGTCGAGGACGACGAAACGATACTCGAAGCCGCGCT
GCGGGAAGGCTTCTCGCTGCCCTACGGCTGCCGCAACGGGGCGTGCGGCTCGTGCAAGGGCACGGTCCTGCAGGGCGAGC
TCGATTATGGCGTCTATCAGCCCAATGCGCTCAAGGACGAGGAGAAGGCGCGCGGACGCGCGCTCTTCTGCCGGGCGAAG
CCGCTGTCCGACATGGCGATCGAAGTCCGCGAGATCGGTGCGAGCAAGGACATCATCGTCAAGACGATGCCGTGCCGCGT
CGAAAAACTCGAGAAGCTCGCCGACGACGTCATGCGCGTGCGCATCAAATTGCCGGCCAACGAGCGCCTGCAATTCCTCG
CCGGGCAGTACATCGATTTCCAGCTCAAGGATGGACGGACGCGCAGCTATTCGCTCGCCAACGCCCCGCATGACGATGCC
CTGCTCGAACTGCACATCCGTCACGTCCCCGGCGGTCTGTTCACCGATCAGGTTTTCACCACGCTCAAGGAACGCGACAT
CCTGCGGCTCAAGGGCCCGCTAGGCAGCTTCTTCATTCGCGAGGATTCCGACAAGCCGATGATTTTCGTCGCCGGTGGCA
CCGGCTTCGCGCCGATCAAGAGCATGCTCGAGCACGCGTTCGCCACGCATACCGACCGCGAGCTCGTGCTTTACTGGGGC
GCGCGTGCGCGCAAGGACCTCTACCTCGCCGAGTTGCCGCAGCAGTGGCTCGCCGAACGGCCCAATTTCAGCTTCATTCC
GGTCCTGTCGCAGGCCGAGCCGGACGACGCCTGGCAAGGCCGCACGGGCTTCGTGCACGAGGCCGTTCTCGCCGATTTCG
CCGACCTTTCCGGCTATGAGGTGTACACCAGCGGCGCCCCGGCGATGGTCGACAGTGCGCGCGAGAGTTTCGTGAATACG
CGCGGGCTGCCCGAGGACGCGTTCTTCGCCGACGCCTTCGTCTACGCCGCGGACACCCTCGCGGCGACGTCCGCGTAA

Upstream 100 bases:

>100_bases
TTACCTGTCGTCGCCGCGTGAGACCCTGGCTCGGCAAGGGTGAAAACCGTGCTGCGCCGGGCGATAATTGCTGGCTGTTC
CCGCATTCTAAGCTCACGCC

Downstream 100 bases:

>100_bases
GGACTACCGCGCCTTACTGCTGCCGCGGGGCCTTACAACGCGCCCTTGGCGCCGGCCTGCATGATCTCGATCAGGCCTTC
GCGCACGGCCTTGATGCGCG

Product: CDP-6-deoxy-delta-3,4-glucoseen reductase

Products: NAD(P); FMNH2; 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: CDP-6-deoxy-delta-3,4-glucoseen reductase; E3 [H]

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MSHQVTIQPSGHRFTVEDDETILEAALREGFSLPYGCRNGACGSCKGTVLQGELDYGVYQPNALKDEEKARGRALFCRAK
PLSDMAIEVREIGASKDIIVKTMPCRVEKLEKLADDVMRVRIKLPANERLQFLAGQYIDFQLKDGRTRSYSLANAPHDDA
LLELHIRHVPGGLFTDQVFTTLKERDILRLKGPLGSFFIREDSDKPMIFVAGGTGFAPIKSMLEHAFATHTDRELVLYWG
ARARKDLYLAELPQQWLAERPNFSFIPVLSQAEPDDAWQGRTGFVHEAVLADFADLSGYEVYTSGAPAMVDSARESFVNT
RGLPEDAFFADAFVYAADTLAATSA

Sequences:

>Translated_345_residues
MSHQVTIQPSGHRFTVEDDETILEAALREGFSLPYGCRNGACGSCKGTVLQGELDYGVYQPNALKDEEKARGRALFCRAK
PLSDMAIEVREIGASKDIIVKTMPCRVEKLEKLADDVMRVRIKLPANERLQFLAGQYIDFQLKDGRTRSYSLANAPHDDA
LLELHIRHVPGGLFTDQVFTTLKERDILRLKGPLGSFFIREDSDKPMIFVAGGTGFAPIKSMLEHAFATHTDRELVLYWG
ARARKDLYLAELPQQWLAERPNFSFIPVLSQAEPDDAWQGRTGFVHEAVLADFADLSGYEVYTSGAPAMVDSARESFVNT
RGLPEDAFFADAFVYAADTLAATSA
>Mature_344_residues
SHQVTIQPSGHRFTVEDDETILEAALREGFSLPYGCRNGACGSCKGTVLQGELDYGVYQPNALKDEEKARGRALFCRAKP
LSDMAIEVREIGASKDIIVKTMPCRVEKLEKLADDVMRVRIKLPANERLQFLAGQYIDFQLKDGRTRSYSLANAPHDDAL
LELHIRHVPGGLFTDQVFTTLKERDILRLKGPLGSFFIREDSDKPMIFVAGGTGFAPIKSMLEHAFATHTDRELVLYWGA
RARKDLYLAELPQQWLAERPNFSFIPVLSQAEPDDAWQGRTGFVHEAVLADFADLSGYEVYTSGAPAMVDSARESFVNTR
GLPEDAFFADAFVYAADTLAATSA

Specific function: Participates in the conversion of CDP-6-deoxy-D-glycero- L-threo-4-hexulose to 3,6-dideoxy-D-glycero-D-glycero-4-hexulose together with CDP-6-deoxy-D-glycero-L-threo-4-hexulose-3-dehydrase (E1) in two consecutive steps. The detailed mechanism of E3 is not

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367314, Length=237, Percent_Identity=35.0210970464135, Blast_Score=145, Evalue=4e-36,
Organism=Escherichia coli, GI1787658, Length=101, Percent_Identity=36.6336633663366, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.5.1.29 [C]

Molecular weight: Translated: 38331; Mature: 38200

Theoretical pI: Translated: 5.15; Mature: 5.15

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHQVTIQPSGHRFTVEDDETILEAALREGFSLPYGCRNGACGSCKGTVLQGELDYGVYQ
CCCEEEEECCCCEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEC
PNALKDEEKARGRALFCRAKPLSDMAIEVREIGASKDIIVKTMPCRVEKLEKLADDVMRV
CCCCCCHHHHCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHEE
RIKLPANERLQFLAGQYIDFQLKDGRTRSYSLANAPHDDALLELHIRHVPGGLFTDQVFT
EEECCCCHHHHHHHCCEEEEEECCCCCCEEEECCCCCCCCEEEEEEEECCCCCHHHHHHH
TLKERDILRLKGPLGSFFIREDSDKPMIFVAGGTGFAPIKSMLEHAFATHTDRELVLYWG
HHHHCCEEEEECCCCCEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEC
ARARKDLYLAELPQQWLAERPNFSFIPVLSQAEPDDAWQGRTGFVHEAVLADFADLSGYE
CCCCCCHHHHHCCHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCEE
VYTSGAPAMVDSARESFVNTRGLPEDAFFADAFVYAADTLAATSA
EEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SHQVTIQPSGHRFTVEDDETILEAALREGFSLPYGCRNGACGSCKGTVLQGELDYGVYQ
CCEEEEECCCCEEEECCCHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCCEEC
PNALKDEEKARGRALFCRAKPLSDMAIEVREIGASKDIIVKTMPCRVEKLEKLADDVMRV
CCCCCCHHHHCCCEEEEECCCCCHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHEE
RIKLPANERLQFLAGQYIDFQLKDGRTRSYSLANAPHDDALLELHIRHVPGGLFTDQVFT
EEECCCCHHHHHHHCCEEEEEECCCCCCEEEECCCCCCCCEEEEEEEECCCCCHHHHHHH
TLKERDILRLKGPLGSFFIREDSDKPMIFVAGGTGFAPIKSMLEHAFATHTDRELVLYWG
HHHHCCEEEEECCCCCEEEEECCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEEEEC
ARARKDLYLAELPQQWLAERPNFSFIPVLSQAEPDDAWQGRTGFVHEAVLADFADLSGYE
CCCCCCHHHHHCCHHHHHCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCCCEE
VYTSGAPAMVDSARESFVNTRGLPEDAFFADAFVYAADTLAATSA
EEECCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD(P)H2; FMN [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; FMN; 2 2-octaprenylphenol; O2 [C]

Specific reaction: NAD(P)H2 + FMN = NAD(P) + FMNH2 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10931327; 11586360; 12142430 [H]