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The map label for this gene is cysG

Identifier: 74318489

GI number: 74318489

Start: 2546872

End: 2548311

Strand: Reverse

Name: cysG

Synonym: Tbd_2471

Alternate gene names: 74318489

Gene position: 2548311-2546872 (Counterclockwise)

Preceding gene: 74318490

Following gene: 74318488

Centisome position: 87.58

GC content: 69.17

Gene sequence:

>1440_bases
ATGAACTATCTGCCGATTTTCCTCGACCTGCGCGACCGTCACTGTCTTGTCGTGGGCGGTTCCGAGACGGCCGCGCGCAA
GGCCGAATTGCTGCTGCGCGCCGGCGCGCACGTCGCCGTCGCCGCGCCGGCCCTGCACGCGGGCTTCGAGCAGTTGCCTG
ACCGGCAGCGGCTCACGCGTGTCGCCGACACCTTCAGCCCCGCGCTGCTCGACGGCAAGGATGCGGTCATCGTCGTCGAG
GACGATGCGGCGGCGGCGCAGATCGTCGCCGACGCTGCGCGCGCGCGTCACCTGCCCGTGAACGTCGCCGACAAGCCGGC
GCTGTGCAGCTTCATCCTGCCGTCGATCATCGACCGCTCGCCGATCATGGTCGCGGTCTCGAGCGGCGGCGAATCGCCGG
TGCTCGCCCGGCTGCTGCGCGCGCGGCTCGAGACGTTGATTCCTGCCGCCTACGGCCGGCTCTCGGCGCTCGCCTCGCGC
TACAAGGCGCGTGTGCGCGAAGCGATCCAGCCTGGGCAGCGCCGCGCCTTCTGGGAAAAGGTCTTCCTCTCGTCGGTCGC
CGAAATGGTGTTTTCCGGGCGCGACAGCGAGGCCGAAGCGCAGCTCGAGGCGATGATCAAGGACAGCGCCGCGCACGAGC
CGGCGCGCGGCGAGGTCTACCTCGTCGGCGCGGGTCCGGGCAACCCCGACCTGCTGACCTTCCGCGCGCTGCGCCTGATG
CAGCAGGCCGACGTCGTCGTCTACGACCGCCTCGTCTCGCAGCCCATCCTCGACATGTGCCGGCGCGACGCCGAACGCAT
CTACGTCGGCAAGGAACGCGACGACCATGCGGTGCCGCAGGAAGAAATCAACATGATGCTCGTGCGCCTCGCCAAGGAGG
GTAAGCGCACGCTGCGCCTCAAGGGTGGCGACCCGTTCATCTTCGGCCGCGGCGGCGAGGAGATCGAGACGCTGGTCGAG
CACGGCGTGGCATTCCAGGTCGTCCCCGGCATCACGGCGGCGGCGGGCGTCGCCTCGTATGCCGGCATTCCTCTGACCCA
TCGCGATTACGCGCAGTCGGTCGCCTTCGTCACCGGTCACCTGAAGGAAAACACCTTCAACATGAACTGGGAGGGCATCG
CGCGCCGCGACCAGACGATCGTCATCTACATGGGGCTGAAGGGCTTGCCGATGCTGTGCGAAGCCTTGATCAAGCACGGG
CTGACCGCCGACACGCCGGCGGCGATCGTCCAGCACGGCACGCTGCCGACGCAGCGTGTCATCACCGGCACGCTCGCGAC
GCTGCCGACGCTCGCCGTGGAGGCCGGCCTCAAGGCACCGACGCTGATCATCGTCGGCAACGTCGTCAAATTGCGCGAGA
AGCTCGCCTGGTACCGGCCGCAGGCCGCCGGCGAAGCGGCCGCCGCCACTCCGCTCGAAGCCCCCGACCACCTCGCCTGA

Upstream 100 bases:

>100_bases
CTCGACTTCGTCGAGATGAACCCCGGCGACATGCGCTTCATCTTCATTCCGCCGTATTCGGCCGATTCCGGCGACGCCGC
CGCTCCTGCCGAGTGAAGCG

Downstream 100 bases:

>100_bases
CCCGGCGATGCCGACCGATCCGCCGCGGCTCGAGAGCCGGCCGGAGATCGAAGCAGAGGTCGCCGCCATGCGCGGGAACG
GGCGGCACATCGCGCCGGTT

Product: uroporphyrinogen-III C-methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MNYLPIFLDLRDRHCLVVGGSETAARKAELLLRAGAHVAVAAPALHAGFEQLPDRQRLTRVADTFSPALLDGKDAVIVVE
DDAAAAQIVADAARARHLPVNVADKPALCSFILPSIIDRSPIMVAVSSGGESPVLARLLRARLETLIPAAYGRLSALASR
YKARVREAIQPGQRRAFWEKVFLSSVAEMVFSGRDSEAEAQLEAMIKDSAAHEPARGEVYLVGAGPGNPDLLTFRALRLM
QQADVVVYDRLVSQPILDMCRRDAERIYVGKERDDHAVPQEEINMMLVRLAKEGKRTLRLKGGDPFIFGRGGEEIETLVE
HGVAFQVVPGITAAAGVASYAGIPLTHRDYAQSVAFVTGHLKENTFNMNWEGIARRDQTIVIYMGLKGLPMLCEALIKHG
LTADTPAAIVQHGTLPTQRVITGTLATLPTLAVEAGLKAPTLIIVGNVVKLREKLAWYRPQAAGEAAAATPLEAPDHLA

Sequences:

>Translated_479_residues
MNYLPIFLDLRDRHCLVVGGSETAARKAELLLRAGAHVAVAAPALHAGFEQLPDRQRLTRVADTFSPALLDGKDAVIVVE
DDAAAAQIVADAARARHLPVNVADKPALCSFILPSIIDRSPIMVAVSSGGESPVLARLLRARLETLIPAAYGRLSALASR
YKARVREAIQPGQRRAFWEKVFLSSVAEMVFSGRDSEAEAQLEAMIKDSAAHEPARGEVYLVGAGPGNPDLLTFRALRLM
QQADVVVYDRLVSQPILDMCRRDAERIYVGKERDDHAVPQEEINMMLVRLAKEGKRTLRLKGGDPFIFGRGGEEIETLVE
HGVAFQVVPGITAAAGVASYAGIPLTHRDYAQSVAFVTGHLKENTFNMNWEGIARRDQTIVIYMGLKGLPMLCEALIKHG
LTADTPAAIVQHGTLPTQRVITGTLATLPTLAVEAGLKAPTLIIVGNVVKLREKLAWYRPQAAGEAAAATPLEAPDHLA
>Mature_479_residues
MNYLPIFLDLRDRHCLVVGGSETAARKAELLLRAGAHVAVAAPALHAGFEQLPDRQRLTRVADTFSPALLDGKDAVIVVE
DDAAAAQIVADAARARHLPVNVADKPALCSFILPSIIDRSPIMVAVSSGGESPVLARLLRARLETLIPAAYGRLSALASR
YKARVREAIQPGQRRAFWEKVFLSSVAEMVFSGRDSEAEAQLEAMIKDSAAHEPARGEVYLVGAGPGNPDLLTFRALRLM
QQADVVVYDRLVSQPILDMCRRDAERIYVGKERDDHAVPQEEINMMLVRLAKEGKRTLRLKGGDPFIFGRGGEEIETLVE
HGVAFQVVPGITAAAGVASYAGIPLTHRDYAQSVAFVTGHLKENTFNMNWEGIARRDQTIVIYMGLKGLPMLCEALIKHG
LTADTPAAIVQHGTLPTQRVITGTLATLPTLAVEAGLKAPTLIIVGNVVKLREKLAWYRPQAAGEAAAATPLEAPDHLA

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family

Homologues:

Organism=Escherichia coli, GI1789768, Length=460, Percent_Identity=52.6086956521739, Blast_Score=460, Evalue=1e-130,
Organism=Saccharomyces cerevisiae, GI6322922, Length=234, Percent_Identity=36.3247863247863, Blast_Score=142, Evalue=8e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSG_THIDA (Q3SG32)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_316229.1
- ProteinModelPortal:   Q3SG32
- SMR:   Q3SG32
- STRING:   Q3SG32
- GeneID:   3671790
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_2471
- NMPDR:   fig|292415.3.peg.2544
- eggNOG:   COG0007
- HOGENOM:   HBG730212
- OMA:   MCRRDAE
- PhylomeDB:   Q3SG32
- BioCyc:   TDEN292415:TBD_2471-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01646
- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- Gene3D:   G3DSA:3.40.1010.10
- Gene3D:   G3DSA:3.30.950.10
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.8.210
- TIGRFAMs:   TIGR01469
- TIGRFAMs:   TIGR01470

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase; SSF53790 Cor/por_Metransf

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4

Molecular weight: Translated: 51742; Mature: 51742

Theoretical pI: Translated: 7.40; Mature: 7.40

Prosite motif: PS00839 SUMT_1; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNYLPIFLDLRDRHCLVVGGSETAARKAELLLRAGAHVAVAAPALHAGFEQLPDRQRLTR
CCCEEEEEEECCCEEEEECCCHHHHHHHHHHHHCCCCEEEECHHHHHHHHHCCCHHHHHH
VADTFSPALLDGKDAVIVVEDDAAAAQIVADAARARHLPVNVADKPALCSFILPSIIDRS
HHHHCCCHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCC
PIMVAVSSGGESPVLARLLRARLETLIPAAYGRLSALASRYKARVREAIQPGQRRAFWEK
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
VFLSSVAEMVFSGRDSEAEAQLEAMIKDSAAHEPARGEVYLVGAGPGNPDLLTFRALRLM
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHH
QQADVVVYDRLVSQPILDMCRRDAERIYVGKERDDHAVPQEEINMMLVRLAKEGKRTLRL
HHCCHHHHHHHHHHHHHHHHHCCHHEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEE
KGGDPFIFGRGGEEIETLVEHGVAFQVVPGITAAAGVASYAGIPLTHRDYAQSVAFVTGH
ECCCEEEECCCCHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LKENTFNMNWEGIARRDQTIVIYMGLKGLPMLCEALIKHGLTADTPAAIVQHGTLPTQRV
HCCCCCCCCCHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHCCCCCHHHH
ITGTLATLPTLAVEAGLKAPTLIIVGNVVKLREKLAWYRPQAAGEAAAATPLEAPDHLA
HHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNYLPIFLDLRDRHCLVVGGSETAARKAELLLRAGAHVAVAAPALHAGFEQLPDRQRLTR
CCCEEEEEEECCCEEEEECCCHHHHHHHHHHHHCCCCEEEECHHHHHHHHHCCCHHHHHH
VADTFSPALLDGKDAVIVVEDDAAAAQIVADAARARHLPVNVADKPALCSFILPSIIDRS
HHHHCCCHHCCCCCEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCC
PIMVAVSSGGESPVLARLLRARLETLIPAAYGRLSALASRYKARVREAIQPGQRRAFWEK
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
VFLSSVAEMVFSGRDSEAEAQLEAMIKDSAAHEPARGEVYLVGAGPGNPDLLTFRALRLM
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHHHH
QQADVVVYDRLVSQPILDMCRRDAERIYVGKERDDHAVPQEEINMMLVRLAKEGKRTLRL
HHCCHHHHHHHHHHHHHHHHHCCHHEEEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEE
KGGDPFIFGRGGEEIETLVEHGVAFQVVPGITAAAGVASYAGIPLTHRDYAQSVAFVTGH
ECCCEEEECCCCHHHHHHHHCCCEEEECCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
LKENTFNMNWEGIARRDQTIVIYMGLKGLPMLCEALIKHGLTADTPAAIVQHGTLPTQRV
HCCCCCCCCCHHHCCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCHHHHHCCCCCHHHH
ITGTLATLPTLAVEAGLKAPTLIIVGNVVKLREKLAWYRPQAAGEAAAATPLEAPDHLA
HHHHHHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA