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The map label for this gene is holA [H]

Identifier: 74318461

GI number: 74318461

Start: 2512184

End: 2513218

Strand: Reverse

Name: holA [H]

Synonym: Tbd_2443

Alternate gene names: 74318461

Gene position: 2513218-2512184 (Counterclockwise)

Preceding gene: 74318462

Following gene: 74318460

Centisome position: 86.37

GC content: 70.05

Gene sequence:

>1035_bases
TTGAACGTCACGAGCGACCGTCTGCCGGAGCAGCTTGCGCGCGGCCTCGCCCCGCTCTACGTGCTGGTCGGCGACGAGCC
GCTGGCGGCGCAGGAAGCGGGCGACGCGATCCGCGCTGCCGCGCGAGCGGCCGGCTATAGCGAGCGCACGGTCCACACCG
CGCAGGGGCGCTACAACTGGCAGGGCATTTTCGCCGGCGCCGACAATCTTTCGCTGTTCGCCGAACGTCGGCTCACCGAA
ATCCGCATTCCCTCGGGCAAGCCCGGCGTCGACGGCGCCAAGGCGCTCGAGGCCTACGCGGCGAAGCTGCCGGCCGACAC
GCTGACGCTCGTCAGCCTGCCCGGGCTCGACAGGAAAACCCAGCAAAGCCGCTGGTTCACCGCGCTGGCCAAAAGCGCCG
TCGTCGTCGAGGCGCGCCCGGTCGAGCGCGCGCAATTGCCGGCGTGGATCGACCGCCGGCTCGCCCGCCAGGGATTGCAC
GCCGAACGCGCGGCGCTCGAATTCCTCGCCGACCAGGTCGAGGGAAATCTCCTGGCCGCGCAGCAGGAGATCGACAAGCT
CGCCCTGCTGTTGCCGCCCGGCGCCGTGACGCTTGCCGATGTCGAGCATGCCGTGGTCGACGTCAGTCGCCTCGAGGCGG
ACGCGCTGCAGGATGCGCTGTACGCCGGCGACGCCGCGCGCTTCGCGCAGGTGGCGAAGGATCTTCAGGACAGCGGCGAG
GCCGTGCCGGCCATCCTGTGGCAGGTGGCGTCGGCGGTGCAGTTGCTGCTCAGGCTAAAATTGGCCCAGTCGAACGGCGA
AAGCCTGCCCGCGCTGATGCGGACGCTGTGGGGACGCGACAAGCAGCGCGCTCCGCAGATCGAGCGCGCACTCAAACGCC
TGAGCATGACGCAGATCGAGGCCGCGCTGGGCGCGCTCGCAGGGGTCGACCGCCAGGCGAAAGGCCTGGACCGCATAGGC
GATCCGTGGGACAGCCTGCTGCGCGCTGCGATGACACTTGCCGGCGGTGAACGCCGCGAAAACAGGACACGATGA

Upstream 100 bases:

>100_bases
TGCTGGCCAAGGGCGCCGAGGAGCAGCTGCTGTACCGCGACATGGAAGACAGCGCGGCGCGGCGGATCCTGCGCCGGCTG
CAGGCGCTCGGCGGCGACAC

Downstream 100 bases:

>100_bases
TGGACGTGAAAAGCTACATGCAGACCCTCGGCAGGCAGGCGCGCGAGGCCTCGCGCGCGATCGCGCGCGCCGACACCGAG
CAGAAGAACCGCGCCTTGCT

Product: DNA polymerase III subunit delta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 344; Mature: 344

Protein sequence:

>344_residues
MNVTSDRLPEQLARGLAPLYVLVGDEPLAAQEAGDAIRAAARAAGYSERTVHTAQGRYNWQGIFAGADNLSLFAERRLTE
IRIPSGKPGVDGAKALEAYAAKLPADTLTLVSLPGLDRKTQQSRWFTALAKSAVVVEARPVERAQLPAWIDRRLARQGLH
AERAALEFLADQVEGNLLAAQQEIDKLALLLPPGAVTLADVEHAVVDVSRLEADALQDALYAGDAARFAQVAKDLQDSGE
AVPAILWQVASAVQLLLRLKLAQSNGESLPALMRTLWGRDKQRAPQIERALKRLSMTQIEAALGALAGVDRQAKGLDRIG
DPWDSLLRAAMTLAGGERRENRTR

Sequences:

>Translated_344_residues
MNVTSDRLPEQLARGLAPLYVLVGDEPLAAQEAGDAIRAAARAAGYSERTVHTAQGRYNWQGIFAGADNLSLFAERRLTE
IRIPSGKPGVDGAKALEAYAAKLPADTLTLVSLPGLDRKTQQSRWFTALAKSAVVVEARPVERAQLPAWIDRRLARQGLH
AERAALEFLADQVEGNLLAAQQEIDKLALLLPPGAVTLADVEHAVVDVSRLEADALQDALYAGDAARFAQVAKDLQDSGE
AVPAILWQVASAVQLLLRLKLAQSNGESLPALMRTLWGRDKQRAPQIERALKRLSMTQIEAALGALAGVDRQAKGLDRIG
DPWDSLLRAAMTLAGGERRENRTR
>Mature_344_residues
MNVTSDRLPEQLARGLAPLYVLVGDEPLAAQEAGDAIRAAARAAGYSERTVHTAQGRYNWQGIFAGADNLSLFAERRLTE
IRIPSGKPGVDGAKALEAYAAKLPADTLTLVSLPGLDRKTQQSRWFTALAKSAVVVEARPVERAQLPAWIDRRLARQGLH
AERAALEFLADQVEGNLLAAQQEIDKLALLLPPGAVTLADVEHAVVDVSRLEADALQDALYAGDAARFAQVAKDLQDSGE
AVPAILWQVASAVQLLLRLKLAQSNGESLPALMRTLWGRDKQRAPQIERALKRLSMTQIEAALGALAGVDRQAKGLDRIG
DPWDSLLRAAMTLAGGERRENRTR

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The delta subunit seems to interact with the gamma subunit to transfer the b

COG id: COG1466

COG function: function code L; DNA polymerase III, delta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786859, Length=307, Percent_Identity=33.8762214983713, Blast_Score=124, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008921
- InterPro:   IPR010372
- InterPro:   IPR005790 [H]

Pfam domain/function: PF06144 DNA_pol3_delta [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 37175; Mature: 37175

Theoretical pI: Translated: 7.77; Mature: 7.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNVTSDRLPEQLARGLAPLYVLVGDEPLAAQEAGDAIRAAARAAGYSERTVHTAQGRYNW
CCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCE
QGIFAGADNLSLFAERRLTEIRIPSGKPGVDGAKALEAYAAKLPADTLTLVSLPGLDRKT
EEEEECCCCHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHH
QQSRWFTALAKSAVVVEARPVERAQLPAWIDRRLARQGLHAERAALEFLADQVEGNLLAA
HHHHHHHHHHHHHEEEECCCCHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHH
QQEIDKLALLLPPGAVTLADVEHAVVDVSRLEADALQDALYAGDAARFAQVAKDLQDSGE
HHHHHHEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
AVPAILWQVASAVQLLLRLKLAQSNGESLPALMRTLWGRDKQRAPQIERALKRLSMTQIE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
AALGALAGVDRQAKGLDRIGDPWDSLLRAAMTLAGGERRENRTR
HHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNVTSDRLPEQLARGLAPLYVLVGDEPLAAQEAGDAIRAAARAAGYSERTVHTAQGRYNW
CCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCCCE
QGIFAGADNLSLFAERRLTEIRIPSGKPGVDGAKALEAYAAKLPADTLTLVSLPGLDRKT
EEEEECCCCHHHHHHHHHHEEECCCCCCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHH
QQSRWFTALAKSAVVVEARPVERAQLPAWIDRRLARQGLHAERAALEFLADQVEGNLLAA
HHHHHHHHHHHHHEEEECCCCHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHH
QQEIDKLALLLPPGAVTLADVEHAVVDVSRLEADALQDALYAGDAARFAQVAKDLQDSGE
HHHHHHEEEEECCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
AVPAILWQVASAVQLLLRLKLAQSNGESLPALMRTLWGRDKQRAPQIERALKRLSMTQIE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHH
AALGALAGVDRQAKGLDRIGDPWDSLLRAAMTLAGGERRENRTR
HHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8505303; 1400251; 8905232; 9278503; 3316191; 8505304; 1575709 [H]