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The map label for this gene is xthA [H]

Identifier: 74318453

GI number: 74318453

Start: 2505845

End: 2506612

Strand: Direct

Name: xthA [H]

Synonym: Tbd_2435

Alternate gene names: 74318453

Gene position: 2505845-2506612 (Clockwise)

Preceding gene: 74318452

Following gene: 74318464

Centisome position: 86.12

GC content: 63.67

Gene sequence:

>768_bases
ATGAAAATCGCCGCCTGGAACGTCAACTCGCTCAAAGTCCGCCTGCCGCAACTGCTGGATTTTCTCGCGACACGCCGGCC
CGACGTCGTCTGCCTGCAGGAAACCAAGGTCCCCGACGACGCCTTTCCGTCCGTCGAGCTGTCGGCGGCGGGGTATCGCG
CGGTCTTTTCCGGGCAGAAAACCTATAACGGCGTCGCGATCCTGAGTCGTTCACAAGCCACGGACGTGAGCGTCGGCATT
CCGGGCTTCGGCGACGAGCAGAAGCGCGTCATCGCGGCGACGGTTGAAGGCACACGCGTCGTCTGCGTCTATTGCCCGAA
CGGCCAGAGCCCCGATTCCGACAAGTATTTCTACAAGCTTGCCTGGTTCGATGCGCTCTCGCTGTGGCTCATGGAGGAGC
TTGCGCGCCACCCAAGGCTGGCCGTCCTCGGCGACTTCAACATCGCGCCTGAAGATCGCGACGTCCACGATCCCAAGGCG
TGGGAAGGTTCGGTGCACGTGTCACCGCGCGAGCGCGAGCGCTTCCAGGCGTTGCTGCAGCTCGGTCTCAAGGACAGCTT
CCGCCTGTTCGAACAGCCGGAGAAGAGTTTCTCGTGGTGGGACTACCGGATGATGGGGTTTCGCCGCAACCACGGCCTTC
GGATCGACCATATCCTGCTAACCGACGCGCTCGCCGCGAGCTGCACCTCGAGCACGATCGACCGCGACATGCGCAAACTC
GAGCGGCCGTCGGACCACGCGCCGGTGCTGGCGGAGATCGGCGTCTAA

Upstream 100 bases:

>100_bases
ACGCCAGCACCTGCACCCGGCGAATAGGCACCCCGGGGACAGCCGCTCGCCGTGCCGCGAGGCGGCGCGCGCTTGGCCAC
GCTGCGGTAAACTCCGCGCC

Downstream 100 bases:

>100_bases
TACTGCACGACCGCAGGGTCCAGGCTGCGCCATAAATACCAGGTCGCGACCGTCCGCCACGGCGCGTGGCGTTCGCCGTG
CGCGCGCAAGACCTTCGGCG

Product: exodeoxyribonuclease III

Products: NA

Alternate protein names: EXO III; Exonuclease III; AP endonuclease VI [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MKIAAWNVNSLKVRLPQLLDFLATRRPDVVCLQETKVPDDAFPSVELSAAGYRAVFSGQKTYNGVAILSRSQATDVSVGI
PGFGDEQKRVIAATVEGTRVVCVYCPNGQSPDSDKYFYKLAWFDALSLWLMEELARHPRLAVLGDFNIAPEDRDVHDPKA
WEGSVHVSPRERERFQALLQLGLKDSFRLFEQPEKSFSWWDYRMMGFRRNHGLRIDHILLTDALAASCTSSTIDRDMRKL
ERPSDHAPVLAEIGV

Sequences:

>Translated_255_residues
MKIAAWNVNSLKVRLPQLLDFLATRRPDVVCLQETKVPDDAFPSVELSAAGYRAVFSGQKTYNGVAILSRSQATDVSVGI
PGFGDEQKRVIAATVEGTRVVCVYCPNGQSPDSDKYFYKLAWFDALSLWLMEELARHPRLAVLGDFNIAPEDRDVHDPKA
WEGSVHVSPRERERFQALLQLGLKDSFRLFEQPEKSFSWWDYRMMGFRRNHGLRIDHILLTDALAASCTSSTIDRDMRKL
ERPSDHAPVLAEIGV
>Mature_255_residues
MKIAAWNVNSLKVRLPQLLDFLATRRPDVVCLQETKVPDDAFPSVELSAAGYRAVFSGQKTYNGVAILSRSQATDVSVGI
PGFGDEQKRVIAATVEGTRVVCVYCPNGQSPDSDKYFYKLAWFDALSLWLMEELARHPRLAVLGDFNIAPEDRDVHDPKA
WEGSVHVSPRERERFQALLQLGLKDSFRLFEQPEKSFSWWDYRMMGFRRNHGLRIDHILLTDALAASCTSSTIDRDMRKL
ERPSDHAPVLAEIGV

Specific function: Major apurinic-apyrimidinic endonuclease of E.coli. It removes the damaged DNA at cytosines and guanines by cleaving on the 3'-side of the AP site by a beta-elimination reaction. It exhibits 3'-5'-exonuclease, 3'-phosphomonoesterase, 3'-repair diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=260, Percent_Identity=33.0769230769231, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI18375503, Length=260, Percent_Identity=33.0769230769231, Blast_Score=120, Evalue=1e-27,
Organism=Homo sapiens, GI18375501, Length=260, Percent_Identity=33.0769230769231, Blast_Score=120, Evalue=1e-27,
Organism=Escherichia coli, GI1788046, Length=264, Percent_Identity=39.3939393939394, Blast_Score=173, Evalue=9e-45,
Organism=Caenorhabditis elegans, GI71989536, Length=261, Percent_Identity=28.3524904214559, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI221330655, Length=263, Percent_Identity=28.5171102661597, Blast_Score=88, Evalue=5e-18,
Organism=Drosophila melanogaster, GI17136678, Length=263, Percent_Identity=28.5171102661597, Blast_Score=87, Evalue=1e-17,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 28809; Mature: 28809

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00726 AP_NUCLEASE_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIAAWNVNSLKVRLPQLLDFLATRRPDVVCLQETKVPDDAFPSVELSAAGYRAVFSGQK
CEEEEECCCHHEEEHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEECCCCEEEEECCCC
TYNGVAILSRSQATDVSVGIPGFGDEQKRVIAATVEGTRVVCVYCPNGQSPDSDKYFYKL
CCCCEEEEECCCCCEEEECCCCCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCCEEEEE
AWFDALSLWLMEELARHPRLAVLGDFNIAPEDRDVHDPKAWEGSVHVSPRERERFQALLQ
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHH
LGLKDSFRLFEQPEKSFSWWDYRMMGFRRNHGLRIDHILLTDALAASCTSSTIDRDMRKL
HCCCHHHHHHHCCCCCCCCHHHEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHH
ERPSDHAPVLAEIGV
CCCCCCCCEEEECCC
>Mature Secondary Structure
MKIAAWNVNSLKVRLPQLLDFLATRRPDVVCLQETKVPDDAFPSVELSAAGYRAVFSGQK
CEEEEECCCHHEEEHHHHHHHHHCCCCCEEEEECCCCCCCCCCCEEECCCCEEEEECCCC
TYNGVAILSRSQATDVSVGIPGFGDEQKRVIAATVEGTRVVCVYCPNGQSPDSDKYFYKL
CCCCEEEEECCCCCEEEECCCCCCCCCCEEEEEEECCCEEEEEECCCCCCCCCCCEEEEE
AWFDALSLWLMEELARHPRLAVLGDFNIAPEDRDVHDPKAWEGSVHVSPRERERFQALLQ
HHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCEEECCHHHHHHHHHHH
LGLKDSFRLFEQPEKSFSWWDYRMMGFRRNHGLRIDHILLTDALAASCTSSTIDRDMRKL
HCCCHHHHHHHCCCCCCCCHHHEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHH
ERPSDHAPVLAEIGV
CCCCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3049539; 9097039; 9278503; 8948651; 7885481 [H]