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The map label for this gene is phbI [H]

Identifier: 74318432

GI number: 74318432

Start: 2484060

End: 2485784

Strand: Direct

Name: phbI [H]

Synonym: Tbd_2414

Alternate gene names: 74318432

Gene position: 2484060-2485784 (Clockwise)

Preceding gene: 74318431

Following gene: 74318437

Centisome position: 85.37

GC content: 66.84

Gene sequence:

>1725_bases
ATGAGCTTCTCCATGCACGGCATCGGCGTCTCGGGCGGCATCGCGATCGGCTATGCCCATCTCGCATCGAGCGCGCGCGT
CGAAGTGCCGCAGTACATGCTCGACCACAAGTACATCAAGGACGAGCTGGCACGCTTCGACGAGGCGATCCTCGCCACCC
GCGCAGAACTCGAAACGCTCCGCAGCCACATCCCGGCGAACGCCCCGGCCGAGTTGTCGGCGTTTCTCGACATGCACCTG
ATGTTCCTCGGCGACTCGATGATTGCCGAGCAGCCCAAGCGGCTGATCCGTGAAACGCAATGCAACGCCGAGTGGGCGCT
TGCGCAGCAGATGGAAGCGCTGGTCGCGCGCTTCGACGAGATCGACGACCCCTACCTCAGGAGCCGGCAGGAAGACGTCG
TGCAGGTGGTGCAGCGCGTGCTGAAGGCGCTGATGGGCCATCCGAGCCATTTGCCGCTCGACGTCGACTTCGACAGCGAG
CGCATCCTGGTCGCGCACGAATTGTCGCCGGCCGACATGGTGATCTTCAAGAACGTGCACTTCGCGGCCTTCGTCACCGA
CCTCGGCGGCACCACCTCGCATACCGCGATTCTCGCCCGCAGCATGGGCATGCCGTCGGTGATGGCGCTGCACAATGCGC
GCGGGCTGATCCGCGACCACGATCTCCTGATCGTCGACGGTCGCGAGGGCGTCGTCATCGTCAATCCCGACGAGTCGGTG
CTCGCCGAATACCGCTTGCGCCAGAACCAGTGGCGCATCGACACCGACAAGCTCAAGCGGCTGAAGACCAGCAAGTCGGC
GACGCTCGACGGTACGCCGGTCGAGCTGATGGCGAACATCGAGCTGCTTTCCGACATCGATGCGGTCAAGGCCGCGGGCG
CGCATGGGATCGGCCTGTTCCGCAGCGAATTCCTGTTCCTCAACCGCAGCGACCTGCCGACCGAGGAGGAGCAATACGAG
TCCTACAAGGCCGTCGCCGAAGCGCTCGACGGCAAGCCGGTGACGATACGCACGCTCGACCTGGGCGCGGACAAGCAGGC
GCCGTGGGGGCACACGGTCGCCGACAACCCTGCGCTCGGCCTGCGCGCGATCCGTCTGTGCCTCGCCGAGCCGGGGCTCT
TCCACACCCAGTTGCGCGCGATCCTGCGGGCCTCGGCACACGGCCGTGTCCGCATGCTGATTCCGATGCTGGCGAACTTC
GTCGAGTTGCGGCAGACGCTGCAGCGCATCGACGAGGCCAAGGACTCGCTACGCCGCGACGGACTGGCCTTCGACGAAGG
CATCCCGGTCGGCGGCATGGTCGAGGTCCCGGCGGCGGCGCTCGCCGCGTCGTTCTTCGCCGACCAGCTCGATTTCCTGT
CGATCGGCACCAACGACCTGATCCAGTACACGCTCGCGATCGACCGCGCCGACGACAGCGTCGCCCATCTCTACGACCCC
TTGCACCCCGCCGTGCTGGGCCTGATCCAGCAGACGATCCGCGCCGGCGCCAAGGCGGGCAAGCCGGTCGCGGTGTGCGG
GGAGATGGCGGGCGATCCCTTGCTGACGCGGCTGCTGCTGGGTCTCGGCCTGCGCACGTTCTCGATGCATCCGGCGAGCC
TGCTGCAGGTCAAGCAGCAGATCTTGCGCTCGCACCTCGCCGAGCTCCCGGCGCTGACCCAGCGGCTGCTGAAGAACACC
GATCCCGACCGCACGCTGACGCTGCTCGGCCGCCTCAACGGCTGA

Upstream 100 bases:

>100_bases
CCAAGGGCAGCACGATCACCGTCGAGGCCGAAGGCGACGACGAGCAGGACGCGGTCGACGCGCTGGCGAAACTGATTTCG
GACGGATTCGGGGAAGAGCG

Downstream 100 bases:

>100_bases
GCCGCGGGCCGCTAGGCCGCGAGGTCGCCGGCGATGCGCACGAGCTCGGCCTTGTCCTGACGCAGCTGATTGATCTCGTC
CTTGATCTGCGCCATGCGGC

Product: phosphoenolpyruvate--protein phosphotransferase

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I; Protein I [H]

Number of amino acids: Translated: 574; Mature: 573

Protein sequence:

>574_residues
MSFSMHGIGVSGGIAIGYAHLASSARVEVPQYMLDHKYIKDELARFDEAILATRAELETLRSHIPANAPAELSAFLDMHL
MFLGDSMIAEQPKRLIRETQCNAEWALAQQMEALVARFDEIDDPYLRSRQEDVVQVVQRVLKALMGHPSHLPLDVDFDSE
RILVAHELSPADMVIFKNVHFAAFVTDLGGTTSHTAILARSMGMPSVMALHNARGLIRDHDLLIVDGREGVVIVNPDESV
LAEYRLRQNQWRIDTDKLKRLKTSKSATLDGTPVELMANIELLSDIDAVKAAGAHGIGLFRSEFLFLNRSDLPTEEEQYE
SYKAVAEALDGKPVTIRTLDLGADKQAPWGHTVADNPALGLRAIRLCLAEPGLFHTQLRAILRASAHGRVRMLIPMLANF
VELRQTLQRIDEAKDSLRRDGLAFDEGIPVGGMVEVPAAALAASFFADQLDFLSIGTNDLIQYTLAIDRADDSVAHLYDP
LHPAVLGLIQQTIRAGAKAGKPVAVCGEMAGDPLLTRLLLGLGLRTFSMHPASLLQVKQQILRSHLAELPALTQRLLKNT
DPDRTLTLLGRLNG

Sequences:

>Translated_574_residues
MSFSMHGIGVSGGIAIGYAHLASSARVEVPQYMLDHKYIKDELARFDEAILATRAELETLRSHIPANAPAELSAFLDMHL
MFLGDSMIAEQPKRLIRETQCNAEWALAQQMEALVARFDEIDDPYLRSRQEDVVQVVQRVLKALMGHPSHLPLDVDFDSE
RILVAHELSPADMVIFKNVHFAAFVTDLGGTTSHTAILARSMGMPSVMALHNARGLIRDHDLLIVDGREGVVIVNPDESV
LAEYRLRQNQWRIDTDKLKRLKTSKSATLDGTPVELMANIELLSDIDAVKAAGAHGIGLFRSEFLFLNRSDLPTEEEQYE
SYKAVAEALDGKPVTIRTLDLGADKQAPWGHTVADNPALGLRAIRLCLAEPGLFHTQLRAILRASAHGRVRMLIPMLANF
VELRQTLQRIDEAKDSLRRDGLAFDEGIPVGGMVEVPAAALAASFFADQLDFLSIGTNDLIQYTLAIDRADDSVAHLYDP
LHPAVLGLIQQTIRAGAKAGKPVAVCGEMAGDPLLTRLLLGLGLRTFSMHPASLLQVKQQILRSHLAELPALTQRLLKNT
DPDRTLTLLGRLNG
>Mature_573_residues
SFSMHGIGVSGGIAIGYAHLASSARVEVPQYMLDHKYIKDELARFDEAILATRAELETLRSHIPANAPAELSAFLDMHLM
FLGDSMIAEQPKRLIRETQCNAEWALAQQMEALVARFDEIDDPYLRSRQEDVVQVVQRVLKALMGHPSHLPLDVDFDSER
ILVAHELSPADMVIFKNVHFAAFVTDLGGTTSHTAILARSMGMPSVMALHNARGLIRDHDLLIVDGREGVVIVNPDESVL
AEYRLRQNQWRIDTDKLKRLKTSKSATLDGTPVELMANIELLSDIDAVKAAGAHGIGLFRSEFLFLNRSDLPTEEEQYES
YKAVAEALDGKPVTIRTLDLGADKQAPWGHTVADNPALGLRAIRLCLAEPGLFHTQLRAILRASAHGRVRMLIPMLANFV
ELRQTLQRIDEAKDSLRRDGLAFDEGIPVGGMVEVPAAALAASFFADQLDFLSIGTNDLIQYTLAIDRADDSVAHLYDPL
HPAVLGLIQQTIRAGAKAGKPVAVCGEMAGDPLLTRLLLGLGLRTFSMHPASLLQVKQQILRSHLAELPALTQRLLKNTD
PDRTLTLLGRLNG

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=570, Percent_Identity=38.0701754385965, Blast_Score=385, Evalue=1e-108,
Organism=Escherichia coli, GI1789193, Length=536, Percent_Identity=34.3283582089552, Blast_Score=261, Evalue=7e-71,
Organism=Escherichia coli, GI48994992, Length=444, Percent_Identity=33.3333333333333, Blast_Score=248, Evalue=6e-67,
Organism=Escherichia coli, GI1788726, Length=474, Percent_Identity=33.3333333333333, Blast_Score=247, Evalue=1e-66,
Organism=Escherichia coli, GI1787994, Length=405, Percent_Identity=26.1728395061728, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI226510935, Length=166, Percent_Identity=27.1084337349398, Blast_Score=69, Evalue=8e-13,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 63103; Mature: 62971

Theoretical pI: Translated: 5.99; Mature: 5.99

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSFSMHGIGVSGGIAIGYAHLASSARVEVPQYMLDHKYIKDELARFDEAILATRAELETL
CCCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSHIPANAPAELSAFLDMHLMFLGDSMIAEQPKRLIRETQCNAEWALAQQMEALVARFDE
HHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHC
IDDPYLRSRQEDVVQVVQRVLKALMGHPSHLPLDVDFDSERILVAHELSPADMVIFKNVH
CCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEEEEECCCCCCEEEEECCH
FAAFVTDLGGTTSHTAILARSMGMPSVMALHNARGLIRDHDLLIVDGREGVVIVNPDESV
HHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHCCCCEECCCEEEEECCCCEEEECCCHHH
LAEYRLRQNQWRIDTDKLKRLKTSKSATLDGTPVELMANIELLSDIDAVKAAGAHGIGLF
HHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHH
RSEFLFLNRSDLPTEEEQYESYKAVAEALDGKPVTIRTLDLGADKQAPWGHTVADNPALG
HHHEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCHH
LRAIRLCLAEPGLFHTQLRAILRASAHGRVRMLIPMLANFVELRQTLQRIDEAKDSLRRD
HHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLAFDEGIPVGGMVEVPAAALAASFFADQLDFLSIGTNDLIQYTLAIDRADDSVAHLYDP
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEEECCCCHHHHHHCC
LHPAVLGLIQQTIRAGAKAGKPVAVCGEMAGDPLLTRLLLGLGLRTFSMHPASLLQVKQQ
HHHHHHHHHHHHHHHCCCCCCCCEEHHHHCCCHHHHHHHHHCCCHHHCCCHHHHHHHHHH
ILRSHLAELPALTQRLLKNTDPDRTLTLLGRLNG
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC
>Mature Secondary Structure 
SFSMHGIGVSGGIAIGYAHLASSARVEVPQYMLDHKYIKDELARFDEAILATRAELETL
CCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RSHIPANAPAELSAFLDMHLMFLGDSMIAEQPKRLIRETQCNAEWALAQQMEALVARFDE
HHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHC
IDDPYLRSRQEDVVQVVQRVLKALMGHPSHLPLDVDFDSERILVAHELSPADMVIFKNVH
CCCHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEECCCCCEEEEEECCCCCCEEEEECCH
FAAFVTDLGGTTSHTAILARSMGMPSVMALHNARGLIRDHDLLIVDGREGVVIVNPDESV
HHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHCCCCEECCCEEEEECCCCEEEECCCHHH
LAEYRLRQNQWRIDTDKLKRLKTSKSATLDGTPVELMANIELLSDIDAVKAAGAHGIGLF
HHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHH
RSEFLFLNRSDLPTEEEQYESYKAVAEALDGKPVTIRTLDLGADKQAPWGHTVADNPALG
HHHEEEEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCHH
LRAIRLCLAEPGLFHTQLRAILRASAHGRVRMLIPMLANFVELRQTLQRIDEAKDSLRRD
HHHHHHHHCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GLAFDEGIPVGGMVEVPAAALAASFFADQLDFLSIGTNDLIQYTLAIDRADDSVAHLYDP
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHEEEEEEEECCCCHHHHHHCC
LHPAVLGLIQQTIRAGAKAGKPVAVCGEMAGDPLLTRLLLGLGLRTFSMHPASLLQVKQQ
HHHHHHHHHHHHHHHCCCCCCCCEEHHHHCCCHHHHHHHHHCCCHHHCCCHHHHHHHHHH
ILRSHLAELPALTQRLLKNTDPDRTLTLLGRLNG
HHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1653223 [H]