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The map label for this gene is rnfF [H]

Identifier: 74318429

GI number: 74318429

Start: 2482279

End: 2483367

Strand: Direct

Name: rnfF [H]

Synonym: Tbd_2411

Alternate gene names: 74318429

Gene position: 2482279-2483367 (Clockwise)

Preceding gene: 74318428

Following gene: 74318430

Centisome position: 85.31

GC content: 70.52

Gene sequence:

>1089_bases
ATGGCCGGAACCGGGCTTGAAACAGTGCGCGCAGTCCGGCTTTTTGATTGCGCCCAGGCAGGCATGAACATGAGCCCCTC
CCCGTATATCCGGCTCGCACGCCACGCCCTCTGGCTGCTTCTGGTGTGGGCGCTCGCCGCGTGCAGCCCGCCTCCTCCGC
TGCATCAGCAGCAGTCCTACGTCTTCGGAACGCTGGTCGACGTCACCGTTTACGGCGCTCCCGAGGCGCAGGCGCGCGAG
GCGACCGCAGCGGTTCTCGCACGCTTCGACGCGCTCCACCGCGAACTTCACGCCTGGCAGCCGAGCGCACTATCGCGACT
CAATGCCACGCTTGCGCGCGGCGAGTCCGCGGCCGTGACTCCGGAACTCGCCGCGATGCTGCACGACGCCCGCGAGCTGT
CGATCCGCTCGCAGCACCTCTTCAACCCGGCCATCGGCGGCCTGGTCGCGCTGTGGGGTTTCCACGCCGACGTTCCGCAG
CGGCGCGTGCCCGATCCGGCGGCGATCGCCGGCTGGGTCAAGGCCCGGCCGCGCATGTCGGACCTCGTCATCGCCAACAA
CACGGTCACCACCTCCAACCGCGCCGTGCAGCTCGATCTCGGCGGCTACGCGAAGGGCCGCGCTCTCGACGACGCGGTCG
CAATTCTGAAGGCGCACGGCATCGCCAACGCGCTCGTCAACATCGGCGGCAACGTGATCGCGCTCGGGGCCCACGGCGAG
CGGCCGTGGCGCGTGGGTATCCAGCACCCGCGCGAGCCGGGCACGCTCGCGACGCTCGACCTCCACGACGGCGAGGCGAT
CGGCACCTCGGGCGATTACCAGCGCTACTTCGAGATCGGCGGGCGCCGCTACAGCCACCTCATCGATCCGCGCACCGGCC
GGCCGGCGACCGGAATGCAGGCCGTCACCGTGCTCTTCGGCGGAGCGCGCGCGGGAACGCTTTCCGACGCGCTGTCGAAG
CCGCTCTTCATCGACGGCCCCGCGCACCTCGCCGAAAACGCGGCGCGGCTCGGCGTGCGCAACTACCTCGCCGTCGACGC
CGCCGGCAAAACCCACGTCTCACCTGACCTCGCTCCCCGCCTTCGCTAG

Upstream 100 bases:

>100_bases
CACATGGCGTCGAAAGCAGGCTGGGACGTGATCGCGCCGACGGGTGCTCAATACCGCGATGCTCCCGATCTCGACGGCCT
GATCGACATGCTTCACGCAC

Downstream 100 bases:

>100_bases
AATGCGGGTTTTGGGCTGACTTTCCGGCAATGATAGGCATCCTCATCGTCGCGCACGGCTCGCTCGCCGACAGTCTGGTC
GAGTGCGCGACCCATGTGCT

Product: putative thaimine biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 362; Mature: 361

Protein sequence:

>362_residues
MAGTGLETVRAVRLFDCAQAGMNMSPSPYIRLARHALWLLLVWALAACSPPPPLHQQQSYVFGTLVDVTVYGAPEAQARE
ATAAVLARFDALHRELHAWQPSALSRLNATLARGESAAVTPELAAMLHDARELSIRSQHLFNPAIGGLVALWGFHADVPQ
RRVPDPAAIAGWVKARPRMSDLVIANNTVTTSNRAVQLDLGGYAKGRALDDAVAILKAHGIANALVNIGGNVIALGAHGE
RPWRVGIQHPREPGTLATLDLHDGEAIGTSGDYQRYFEIGGRRYSHLIDPRTGRPATGMQAVTVLFGGARAGTLSDALSK
PLFIDGPAHLAENAARLGVRNYLAVDAAGKTHVSPDLAPRLR

Sequences:

>Translated_362_residues
MAGTGLETVRAVRLFDCAQAGMNMSPSPYIRLARHALWLLLVWALAACSPPPPLHQQQSYVFGTLVDVTVYGAPEAQARE
ATAAVLARFDALHRELHAWQPSALSRLNATLARGESAAVTPELAAMLHDARELSIRSQHLFNPAIGGLVALWGFHADVPQ
RRVPDPAAIAGWVKARPRMSDLVIANNTVTTSNRAVQLDLGGYAKGRALDDAVAILKAHGIANALVNIGGNVIALGAHGE
RPWRVGIQHPREPGTLATLDLHDGEAIGTSGDYQRYFEIGGRRYSHLIDPRTGRPATGMQAVTVLFGGARAGTLSDALSK
PLFIDGPAHLAENAARLGVRNYLAVDAAGKTHVSPDLAPRLR
>Mature_361_residues
AGTGLETVRAVRLFDCAQAGMNMSPSPYIRLARHALWLLLVWALAACSPPPPLHQQQSYVFGTLVDVTVYGAPEAQAREA
TAAVLARFDALHRELHAWQPSALSRLNATLARGESAAVTPELAAMLHDARELSIRSQHLFNPAIGGLVALWGFHADVPQR
RVPDPAAIAGWVKARPRMSDLVIANNTVTTSNRAVQLDLGGYAKGRALDDAVAILKAHGIANALVNIGGNVIALGAHGER
PWRVGIQHPREPGTLATLDLHDGEAIGTSGDYQRYFEIGGRRYSHLIDPRTGRPATGMQAVTVLFGGARAGTLSDALSKP
LFIDGPAHLAENAARLGVRNYLAVDAAGKTHVSPDLAPRLR

Specific function: Required for nitrogen fixation [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rseC family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=312, Percent_Identity=28.2051282051282, Blast_Score=108, Evalue=7e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007359
- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE; PF04246 RseC_MucC [H]

EC number: NA

Molecular weight: Translated: 38611; Mature: 38479

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGTGLETVRAVRLFDCAQAGMNMSPSPYIRLARHALWLLLVWALAACSPPPPLHQQQSY
CCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
VFGTLVDVTVYGAPEAQAREATAAVLARFDALHRELHAWQPSALSRLNATLARGESAAVT
HEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCC
PELAAMLHDARELSIRSQHLFNPAIGGLVALWGFHADVPQRRVPDPAAIAGWVKARPRMS
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCC
DLVIANNTVTTSNRAVQLDLGGYAKGRALDDAVAILKAHGIANALVNIGGNVIALGAHGE
CEEEECCEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
RPWRVGIQHPREPGTLATLDLHDGEAIGTSGDYQRYFEIGGRRYSHLIDPRTGRPATGMQ
CCEEECCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHCCCHHHHHCCCCCCCCCCCHH
AVTVLFGGARAGTLSDALSKPLFIDGPAHLAENAARLGVRNYLAVDAAGKTHVSPDLAPR
EEEEEECCCCCCCHHHHHCCCEEECCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCC
LR
CC
>Mature Secondary Structure 
AGTGLETVRAVRLFDCAQAGMNMSPSPYIRLARHALWLLLVWALAACSPPPPLHQQQSY
CCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
VFGTLVDVTVYGAPEAQAREATAAVLARFDALHRELHAWQPSALSRLNATLARGESAAVT
HEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCC
PELAAMLHDARELSIRSQHLFNPAIGGLVALWGFHADVPQRRVPDPAAIAGWVKARPRMS
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHCCCCCC
DLVIANNTVTTSNRAVQLDLGGYAKGRALDDAVAILKAHGIANALVNIGGNVIALGAHGE
CEEEECCEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCC
RPWRVGIQHPREPGTLATLDLHDGEAIGTSGDYQRYFEIGGRRYSHLIDPRTGRPATGMQ
CCEEECCCCCCCCCCEEEEECCCCCCCCCCCCHHHHHHHCCCHHHHHCCCCCCCCCCCHH
AVTVLFGGARAGTLSDALSKPLFIDGPAHLAENAARLGVRNYLAVDAAGKTHVSPDLAPR
EEEEEECCCCCCCHHHHHCCCEEECCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCCC
LR
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8264535; 2071578 [H]