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The map label for this gene is gshB [H]

Identifier: 74318427

GI number: 74318427

Start: 2480897

End: 2481841

Strand: Direct

Name: gshB [H]

Synonym: Tbd_2409

Alternate gene names: 74318427

Gene position: 2480897-2481841 (Clockwise)

Preceding gene: 74318426

Following gene: 74318428

Centisome position: 85.26

GC content: 68.57

Gene sequence:

>945_bases
ATGAAGCTGCTGTTCGTCGTCGATCCGCTGGCGAGCCTCAAGCCGTACAAGGACAGTTCGGTCGCGATGATGCGCGCGGC
CTGTGCGCGCGGCCACGCCGTGTTCGCGGCCGAAGCACGCGCGCTGCTGGTGCGCGACGGAGTCGCGCGGTCGCGCGCCG
ACGCCGTCGAGACGCGCGGCGACGACGATTGGTATCGCGTGACCGAAACGCGCGAATTCGCGCTCACGGATTTCGATGCC
GTTGTGATGCGCGCCGACCCGCCGGTCGACGTCGACTACCTGCTCGCGACCCACCTGCTCGGCGTCGCCGAGACCAACGG
CGCGCGCGTGCTGAACCGGCCGCGCGCGCTGCGCGACTTCAACGAAAAACTCGCCATCCTCGAGTTTCCGCAATTTGTCG
CCCCGACGCTGGTTTCGGCCGACGCGACAGAAATCGCCCACTTCCTCGCCGCCCACGCCGACATCATCGTCAAGCCGCTC
ACCGAGATGGGCGGCAGCGGCGTCTTCCGCCTCGGCGTTTCCGACCCGAACCGCAACGCCATCCTCGAAACGCTCACCCG
ACGCGGCAGCCGGCCGATCATGGCGCAGCGTTATCTGCCGGCGATCAGCGAAGGCGACAAGCGCATCCTGCTGATCGACG
GCGAGGTGGTGCCGTGGGCCCTCGCGCGGATTCCGCTGACGGGCGAGACGCGCGGCAATCTCGCCGCGGGCGGCACGGCG
CGTGCCCAGCCGCTTTCGGAACGCGACCGCGAGATCGCCGAGACGATCGCGCCCTGGGTGCGCTCGCAAGGCATCTTCCT
CGCCGGCCTCGACGTGATCGGCGACTGCCTCACCGAAATCAACGTCACGAGCCCGACCGGCTTTCAGGAAATCACGGCGC
AGAGCGGCCACGACGTCGCGGACCAGTTCATCGCCGCGATCGAGCGTGCCACGCGTCCGGAATAA

Upstream 100 bases:

>100_bases
CCGACGCCAGCCCCAACCGCTTCTACGCCTACGGCGTCGTCGCGCGCCTGGCGATGCTCGCCGCCTCGATCGAACTCGAC
GAAGCCTTGAACGAGACGGC

Downstream 100 bases:

>100_bases
GGCCGGTGCGGTGACCAGCATCCTCGGCACCTGCCTGTGCGGCCAGATCGAATTCAGGGTATCCGGCCCGTTTCCCGGGC
TCTATCAATGTCATTGCTCC

Product: glutathione synthase

Products: NA

Alternate protein names: GSH synthetase; GSH-S; GSHase; Glutathione synthase [H]

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKLLFVVDPLASLKPYKDSSVAMMRAACARGHAVFAAEARALLVRDGVARSRADAVETRGDDDWYRVTETREFALTDFDA
VVMRADPPVDVDYLLATHLLGVAETNGARVLNRPRALRDFNEKLAILEFPQFVAPTLVSADATEIAHFLAAHADIIVKPL
TEMGGSGVFRLGVSDPNRNAILETLTRRGSRPIMAQRYLPAISEGDKRILLIDGEVVPWALARIPLTGETRGNLAAGGTA
RAQPLSERDREIAETIAPWVRSQGIFLAGLDVIGDCLTEINVTSPTGFQEITAQSGHDVADQFIAAIERATRPE

Sequences:

>Translated_314_residues
MKLLFVVDPLASLKPYKDSSVAMMRAACARGHAVFAAEARALLVRDGVARSRADAVETRGDDDWYRVTETREFALTDFDA
VVMRADPPVDVDYLLATHLLGVAETNGARVLNRPRALRDFNEKLAILEFPQFVAPTLVSADATEIAHFLAAHADIIVKPL
TEMGGSGVFRLGVSDPNRNAILETLTRRGSRPIMAQRYLPAISEGDKRILLIDGEVVPWALARIPLTGETRGNLAAGGTA
RAQPLSERDREIAETIAPWVRSQGIFLAGLDVIGDCLTEINVTSPTGFQEITAQSGHDVADQFIAAIERATRPE
>Mature_314_residues
MKLLFVVDPLASLKPYKDSSVAMMRAACARGHAVFAAEARALLVRDGVARSRADAVETRGDDDWYRVTETREFALTDFDA
VVMRADPPVDVDYLLATHLLGVAETNGARVLNRPRALRDFNEKLAILEFPQFVAPTLVSADATEIAHFLAAHADIIVKPL
TEMGGSGVFRLGVSDPNRNAILETLTRRGSRPIMAQRYLPAISEGDKRILLIDGEVVPWALARIPLTGETRGNLAAGGTA
RAQPLSERDREIAETIAPWVRSQGIFLAGLDVIGDCLTEINVTSPTGFQEITAQSGHDVADQFIAAIERATRPE

Specific function: Glutathione biosynthesis; second step. [C]

COG id: COG0189

COG function: function code HJ; Glutathione synthase/Ribosomal protein S6 modification enzyme (glutaminyl transferase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1789316, Length=309, Percent_Identity=44.336569579288, Blast_Score=272, Evalue=2e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR006284
- InterPro:   IPR004218
- InterPro:   IPR004215
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF02955 GSH-S_ATP; PF02951 GSH-S_N [H]

EC number: =6.3.2.3 [H]

Molecular weight: Translated: 34123; Mature: 34123

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLFVVDPLASLKPYKDSSVAMMRAACARGHAVFAAEARALLVRDGVARSRADAVETRG
CEEEEEECCHHHCCCCCCCHHHHHHHHHHCCCEEEHHHHHHHHHHCCCHHHHHHHHHCCC
DDDWYRVTETREFALTDFDAVVMRADPPVDVDYLLATHLLGVAETNGARVLNRPRALRDF
CCCEEEEECCCEEEEECCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHCCCHHHHHH
NEKLAILEFPQFVAPTLVSADATEIAHFLAAHADIIVKPLTEMGGSGVFRLGVSDPNRNA
HHCEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHH
ILETLTRRGSRPIMAQRYLPAISEGDKRILLIDGEVVPWALARIPLTGETRGNLAAGGTA
HHHHHHHCCCCCCHHHHHCCCCCCCCCEEEEECCCCCCHHHHCCCCCCCCCCCEECCCCC
RAQPLSERDREIAETIAPWVRSQGIFLAGLDVIGDCLTEINVTSPTGFQEITAQSGHDVA
CCCCHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHCCCCCCCCHHHHHHCCCCHHH
DQFIAAIERATRPE
HHHHHHHHHHCCCC
>Mature Secondary Structure
MKLLFVVDPLASLKPYKDSSVAMMRAACARGHAVFAAEARALLVRDGVARSRADAVETRG
CEEEEEECCHHHCCCCCCCHHHHHHHHHHCCCEEEHHHHHHHHHHCCCHHHHHHHHHCCC
DDDWYRVTETREFALTDFDAVVMRADPPVDVDYLLATHLLGVAETNGARVLNRPRALRDF
CCCEEEEECCCEEEEECCEEEEEECCCCCCHHHHHHHHHHHHCCCCCCHHHCCCHHHHHH
NEKLAILEFPQFVAPTLVSADATEIAHFLAAHADIIVKPLTEMGGSGVFRLGVSDPNRNA
HHCEEEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCHHH
ILETLTRRGSRPIMAQRYLPAISEGDKRILLIDGEVVPWALARIPLTGETRGNLAAGGTA
HHHHHHHCCCCCCHHHHHCCCCCCCCCEEEEECCCCCCHHHHCCCCCCCCCCCEECCCCC
RAQPLSERDREIAETIAPWVRSQGIFLAGLDVIGDCLTEINVTSPTGFQEITAQSGHDVA
CCCCHHHHHHHHHHHHHHHHHCCCEEEECHHHHHHHHHHCCCCCCCCHHHHHHCCCCHHH
DQFIAAIERATRPE
HHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]