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The map label for this gene is cyaE [H]

Identifier: 74318339

GI number: 74318339

Start: 2396006

End: 2397391

Strand: Reverse

Name: cyaE [H]

Synonym: Tbd_2321

Alternate gene names: 74318339

Gene position: 2397391-2396006 (Counterclockwise)

Preceding gene: 74318340

Following gene: 74318338

Centisome position: 82.39

GC content: 73.74

Gene sequence:

>1386_bases
TTGAAGTCCTCCCTGTTCATTCTCTGGACGGCTGCGAGCGCCGGCATCGTCCACGCCGCCGCGCTCGAACCGCCTGCGCG
GCCGGGTAGCTTCTGGCTGCCACCGCACGCCGTGGCCGAGCCGGCGCCGGCCATCGCGGCGCCGGTCCGTTTCGACAGCC
TCGCGGCCTTGACCGATCACGCCTTGCGCGAGCGCGCCGAATCGCGCGCGGCGTGGCTCGCGGCGCAGGCCGAGGCGGCT
CGCCTCGACGCAGCGACAGCCGAACTGTGGCCCAGCGTGACGGGGCAATTCAACTTCACGCGCAGCCGTGGCCTGTCGAG
TTCGGGCGCGCCGGTCGCCACCCTGCATCGTTACGGGCCGAGTCTGAGCCTGAGCTACGTGCTGTTCGACTTCGGGGCGC
GCGCGGCAGGCGTCGACGCCCAGCGCTACCGGTTGATCGCGAGTCTGCTATCGGCCAACCGCGCGCTCCAGGACGTGATT
GCCGCGGTCGAGGCCGCGTATTACGGCGTGCAGGCGGCGGCAGCCCTGGTCGACGCCCAGGCGCAGCAGGAAGCCGCCCT
GCGCACGAGTCTCGAGGCCGTCGAGGTCAGACTCCAGGCCGGACTCGCGGCGCGCGCCGATCGGCTGCGCGCCGAGGCGG
CGCTGGTCGAGGCGCAACTCGCGCGCGAGGCCGCGGAACGCGACCAGGCAAACGCCATGGCGACGCTGAAGCAGGCCGCG
GGAATCGCGCAGACCCAGCCGCTGACGCTGGCCTGGGACGTCGCGCCGCCAGCCTTGCCGGAGGTCGACGCACTGCTTGC
CGACCTGCTCGCCGAGGCCGAGCGGCAGCGCCCCGATCTGCATGCGCTGCAGGCGACCGCGGCCAGCGCGCGGGCAGTGG
CCGAGCGCGCCCGCGCCGCGCGCTGGCCGAGCGTGTCGCTCGCGGCGAACAGCGGCCGTACGTTTTTTCTCGAGGACGAA
CGGGTGCCATCGACGACGTATAGCGTCGGCCTCAGCGTCGCGCTTCCCCTATTCGACGGCGGCCGGCTCGCCGCCGAAGC
GCGCGCGGCGGCGCGCGAGGCCGAGCGTCTGCAGGCCGAGGCCGAGGTGCAGCGCAGCCAGGTCGCGCTCGCGATCACGC
GCGCCTACCACGACGTGCGTCACGCCCAGTCGCGCCGCGCCGGCGTCGCGGTGCAGTTCGAGAGCGCTGCCGAGTCGGCA
CGCGCAGCCGAGGCGCGCTACCGCGCCGGGGTGGGCAGCCTGCTTGAATGGCTGACCGCACAGGCCGACCTCGCACGCGC
CCGCCAGGTGCGCGCCCAGTCCGACAGCGACTGGCTCGCCGCGTTTTCGGCCCTCAATCACGCGCTCGGACGTTTGCCGG
GCGCCTCACCGGGAACGCCGCCATGA

Upstream 100 bases:

>100_bases
AGCTGTAGGCGGCCATGCCCGTTGCGGCCCTGCCGAGGCGGCCGTGAGCGGGCACCCGGGGCGGTGGGGCGCCTGTGAAG
TCGTTTAGAATTCAAACACC

Downstream 100 bases:

>100_bases
AACTGCGCATTCTTGTGTCGATCGTCGTGCTGGCGCTGCTCGCTGCGGTCGTTTATCGCGTCCAGCAGCAAGGCGCGGGC
GAGCGCCGCGGCAGCGGCGA

Product: putative secretion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MKSSLFILWTAASAGIVHAAALEPPARPGSFWLPPHAVAEPAPAIAAPVRFDSLAALTDHALRERAESRAAWLAAQAEAA
RLDAATAELWPSVTGQFNFTRSRGLSSSGAPVATLHRYGPSLSLSYVLFDFGARAAGVDAQRYRLIASLLSANRALQDVI
AAVEAAYYGVQAAAALVDAQAQQEAALRTSLEAVEVRLQAGLAARADRLRAEAALVEAQLAREAAERDQANAMATLKQAA
GIAQTQPLTLAWDVAPPALPEVDALLADLLAEAERQRPDLHALQATAASARAVAERARAARWPSVSLAANSGRTFFLEDE
RVPSTTYSVGLSVALPLFDGGRLAAEARAAAREAERLQAEAEVQRSQVALAITRAYHDVRHAQSRRAGVAVQFESAAESA
RAAEARYRAGVGSLLEWLTAQADLARARQVRAQSDSDWLAAFSALNHALGRLPGASPGTPP

Sequences:

>Translated_461_residues
MKSSLFILWTAASAGIVHAAALEPPARPGSFWLPPHAVAEPAPAIAAPVRFDSLAALTDHALRERAESRAAWLAAQAEAA
RLDAATAELWPSVTGQFNFTRSRGLSSSGAPVATLHRYGPSLSLSYVLFDFGARAAGVDAQRYRLIASLLSANRALQDVI
AAVEAAYYGVQAAAALVDAQAQQEAALRTSLEAVEVRLQAGLAARADRLRAEAALVEAQLAREAAERDQANAMATLKQAA
GIAQTQPLTLAWDVAPPALPEVDALLADLLAEAERQRPDLHALQATAASARAVAERARAARWPSVSLAANSGRTFFLEDE
RVPSTTYSVGLSVALPLFDGGRLAAEARAAAREAERLQAEAEVQRSQVALAITRAYHDVRHAQSRRAGVAVQFESAAESA
RAAEARYRAGVGSLLEWLTAQADLARARQVRAQSDSDWLAAFSALNHALGRLPGASPGTPP
>Mature_461_residues
MKSSLFILWTAASAGIVHAAALEPPARPGSFWLPPHAVAEPAPAIAAPVRFDSLAALTDHALRERAESRAAWLAAQAEAA
RLDAATAELWPSVTGQFNFTRSRGLSSSGAPVATLHRYGPSLSLSYVLFDFGARAAGVDAQRYRLIASLLSANRALQDVI
AAVEAAYYGVQAAAALVDAQAQQEAALRTSLEAVEVRLQAGLAARADRLRAEAALVEAQLAREAAERDQANAMATLKQAA
GIAQTQPLTLAWDVAPPALPEVDALLADLLAEAERQRPDLHALQATAASARAVAERARAARWPSVSLAANSGRTFFLEDE
RVPSTTYSVGLSVALPLFDGGRLAAEARAAAREAERLQAEAEVQRSQVALAITRAYHDVRHAQSRRAGVAVQFESAAESA
RAAEARYRAGVGSLLEWLTAQADLARARQVRAQSDSDWLAAFSALNHALGRLPGASPGTPP

Specific function: CyaE is necessary for transport of calmodulin-sensitive adenylate cyclase-hemolysin (cyclolysin) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

None

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 48621; Mature: 48621

Theoretical pI: Translated: 6.98; Mature: 6.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
0.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSSLFILWTAASAGIVHAAALEPPARPGSFWLPPHAVAEPAPAIAAPVRFDSLAALTDH
CCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHH
ALRERAESRAAWLAAQAEAARLDAATAELWPSVTGQFNFTRSRGLSSSGAPVATLHRYGP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHCCCCCCCCCHHHHHHCCC
SLSLSYVLFDFGARAAGVDAQRYRLIASLLSANRALQDVIAAVEAAYYGVQAAAALVDAQ
CCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQQEAALRTSLEAVEVRLQAGLAARADRLRAEAALVEAQLAREAAERDQANAMATLKQAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GIAQTQPLTLAWDVAPPALPEVDALLADLLAEAERQRPDLHALQATAASARAVAERARAA
CCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RWPSVSLAANSGRTFFLEDERVPSTTYSVGLSVALPLFDGGRLAAEARAAAREAERLQAE
CCCCEEEEECCCCEEEEECCCCCCHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
AEVQRSQVALAITRAYHDVRHAQSRRAGVAVQFESAAESARAAEARYRAGVGSLLEWLTA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
QADLARARQVRAQSDSDWLAAFSALNHALGRLPGASPGTPP
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MKSSLFILWTAASAGIVHAAALEPPARPGSFWLPPHAVAEPAPAIAAPVRFDSLAALTDH
CCCCEEEEEECCCCCEEEEECCCCCCCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHHH
ALRERAESRAAWLAAQAEAARLDAATAELWPSVTGQFNFTRSRGLSSSGAPVATLHRYGP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHCCCCCCCCCHHHHHHCCC
SLSLSYVLFDFGARAAGVDAQRYRLIASLLSANRALQDVIAAVEAAYYGVQAAAALVDAQ
CCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQQEAALRTSLEAVEVRLQAGLAARADRLRAEAALVEAQLAREAAERDQANAMATLKQAA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
GIAQTQPLTLAWDVAPPALPEVDALLADLLAEAERQRPDLHALQATAASARAVAERARAA
CCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
RWPSVSLAANSGRTFFLEDERVPSTTYSVGLSVALPLFDGGRLAAEARAAAREAERLQAE
CCCCEEEEECCCCEEEEECCCCCCHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
AEVQRSQVALAITRAYHDVRHAQSRRAGVAVQFESAAESARAAEARYRAGVGSLLEWLTA
HHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHH
QADLARARQVRAQSDSDWLAAFSALNHALGRLPGASPGTPP
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2905265; 12910271 [H]