Click here to switch to the map view.

The map label for this gene is nasD [H]

Identifier: 74318327

GI number: 74318327

Start: 2378968

End: 2381424

Strand: Reverse

Name: nasD [H]

Synonym: Tbd_2309

Alternate gene names: 74318327

Gene position: 2381424-2378968 (Counterclockwise)

Preceding gene: 74318328

Following gene: 74318326

Centisome position: 81.84

GC content: 65.65

Gene sequence:

>2457_bases
ATGGATATGAGTACGCCCATGGGGCAGGGCCCCAAGATGAAGCTCGTGATGGTCGGCAACGGCATGGCCGGCGTGCGCAC
GCTCGAAGAACTGCTGAAGCTTGCACCCGAGCTTTACGACATCACCGTATTCGGCGCCGAGCCGCATCCCAATTACAACC
GCATCCTGCTCTCGCCGGTGCTCGCCGGCGAGCAGACCGTCGATGACATCATCCTCAACGGCGCCGACTGGTACAGCGAG
AACGGCATCCGCCTCCACCTCGGGCAGAAGGTCGTCAGGATCGACCGCGCTGCGCGCGAAGTCGAGACTGCCGACGGCAC
GCGCGCGCGCTACGACCGGCTGCTGCTCGCGACCGGCAGCACGCCCTTCATCCTGCCGGTAGCCGGCGCCGATCTTCCCG
GCGTCGTGAGCTTTCGCGACATCGCCGACGTCGACGCGATGGTCGACGCCGCGGGCCGCTACAAGCATGCGGTCGTGATC
GGCGGAGGGCTCCTCGGGCTCGAGGCCGCCAACGGCCTCATGAAGCGCGGCATGGACGTGACCGTCGTGCACATCGGCCC
GTGGCTCATGGAGCGCCAGCTCGACGAACCCGCCGCGCGCCTGTTGCAGAGAAGCCTCGAAGAGAAAGGCATGAAGTTCC
TGCTGCAGAAACACACGGCTGAACTCGTCGCGGGCGCGTCCGGCCGCGTCACCGGGGTCCGCTTCAAGGACGGCGATGCG
ATTCCCGCCGACCTCGTCGTGATGGCGGTCGGCATCCGCCCCAACACCGAACTCGCCGAGTCCGCCGGCCTGCACTGCAA
TCGCGGCATCGTCGTCAACGACACGATGCAGACCTTCGACCCGCGCATCTACTCGATCGGCGAATGCGTCGCCCACCGCG
GCGTCGCCTACGGCCTGGTCGCGCCGCTGTTTGAACAGGCCAAGGTCTGCGCCAATCATCTCGCGCACTACGGCATCGGC
CGCTACCAGGGCTCGGCGACGTCGACCAAGCTCAAGGTCACCGGCGTCGACCTCTTCTCGGCCGGGGACTTCATGGGTGG
CGAGGGCACCGAGACGATCCTCTATTCCGACCCGATCGGCGGCGTGTACAAGAAACTCGTGATCCGCGACGACAAGCTCG
TCGGCGGCGTCATGGTCGGCGACACCGTCGACGGCGCGTGGTATTTCTCGCTGCTGCGCGACGGCAAGGACATCGGCGCG
CTGCGCGACCATCTGATGTTCGGCCAGGATCATTGCGGCAATCTCGGCCACCAGGGCATCAACCGCGCCGCCGCGATGAC
CGACGACATGGAAGTCTGCGGCTGCAATGGCATCTGCAAAGGCGACATCGTCAAGGCGATCCGCGAGAAGGGCTTGTTCA
CGCTCGAGGACGTGCGTAAGCACACCAAGGCGTCGTCCTCGTGCGGCTCGTGCACGGGCCTCGTCGAGCAGATCCTCGCG
GTCACCGCAGGCGGTGACTACTCGGCCGCGCCGAAAACCAAATCGATGTGCGGCTGCACCGATCACACCCACGAGGCCGT
TCGCGCGGCGATCCGTGAACACCGGCTGCTGACGATTCCGCAGGCGATGGCCTTCCTCGAATGGCGCACACCCAACGGCT
GCGCCTCGTGCCGGCCCGCGCTCAACTACTACCTGATCTCGACCTGGCCGGGCGAGGCCGAGGACGATCCGCAGTCGCGC
TTCATCAACGAGCGCGCCCACGCCAACATCCAGAAGGACGGCAGCTATTCGGTCATCCCGCGCATGTGGGGCGGGCAGAC
GACCCCGGCCGAATTGCGCCGCATCGCCGAAGTCGCCGAGAAGTACGCCGTGCCTTCTGTCAAGGTCACCGGCGGCCAGC
GCATCGACCTTCTGGGCGTCAAGAAGGAAGACCTGCCCAAGATCTGGGCCGACCTCGACATGCCGTCGGGCCACGCCTAC
GGAAAAGCGCTGCGCACCGTGAAGACCTGCGTTGGCTCGGAGTGGTGCCGCTTCGGCACTCAGGACTCGACGCAGATGGG
CATCGATCTCGAAAAGGTGTTCTACAAGATGTGGGCGCCGCACAAGGTCAAGCTCGCGGTCTCGGGCTGCCCGCGCAATT
GCGCCGAAGTCGCGATCAAGGACGTCGGCGTCATCGGCGTCGACTCGGGCTGGGAAATCTACGTCGGCGGCAACGGCGGC
ATCAAGACCGAGGTCGCGCAATTCCTCGTCAAGGTCAAGACGCCCGACGAAGTGCTGGAATATTCCGGCGCCTTCCTCCA
GCTCTATCGCGAAGAGGCGCGCTACCTCGACCGCACCGTGCATTACCTCGAACGCGTCGGCCTCGACTACGTCAAGAAGA
AAGTCGTCGACGACGCCGCGAGCAGGTGCGCCCTCTACGAGCGCCTGCTGTTCGCGCTCTCGGTCGAGCGCGACCCGTGG
CTCGAGCGCGCCCAGGAAGGCAAGCTGAAGCACGAATTCGAAACCATTTCCGCCTGA

Upstream 100 bases:

>100_bases
GCCGCGGCATGAGCGGCCGGCAGGTACGCGCGCGGCAACCGTGGATCCGGCGCAACGGCGTGCCGGTCCTCACTTTTGGC
TATTCGTTTGGAGTGGTGAG

Downstream 100 bases:

>100_bases
GCGCAAAAGGACAGACAGCATGACCCAGTGGACAGACGTTCTCGACCTCAACAACATCCCCCGGCTCGGCGCGCGCATCG
TGCGCCACGGCGCGATCGAC

Product: assimilatory nitrite reductase (NAD(P)H) large subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 818; Mature: 818

Protein sequence:

>818_residues
MDMSTPMGQGPKMKLVMVGNGMAGVRTLEELLKLAPELYDITVFGAEPHPNYNRILLSPVLAGEQTVDDIILNGADWYSE
NGIRLHLGQKVVRIDRAAREVETADGTRARYDRLLLATGSTPFILPVAGADLPGVVSFRDIADVDAMVDAAGRYKHAVVI
GGGLLGLEAANGLMKRGMDVTVVHIGPWLMERQLDEPAARLLQRSLEEKGMKFLLQKHTAELVAGASGRVTGVRFKDGDA
IPADLVVMAVGIRPNTELAESAGLHCNRGIVVNDTMQTFDPRIYSIGECVAHRGVAYGLVAPLFEQAKVCANHLAHYGIG
RYQGSATSTKLKVTGVDLFSAGDFMGGEGTETILYSDPIGGVYKKLVIRDDKLVGGVMVGDTVDGAWYFSLLRDGKDIGA
LRDHLMFGQDHCGNLGHQGINRAAAMTDDMEVCGCNGICKGDIVKAIREKGLFTLEDVRKHTKASSSCGSCTGLVEQILA
VTAGGDYSAAPKTKSMCGCTDHTHEAVRAAIREHRLLTIPQAMAFLEWRTPNGCASCRPALNYYLISTWPGEAEDDPQSR
FINERAHANIQKDGSYSVIPRMWGGQTTPAELRRIAEVAEKYAVPSVKVTGGQRIDLLGVKKEDLPKIWADLDMPSGHAY
GKALRTVKTCVGSEWCRFGTQDSTQMGIDLEKVFYKMWAPHKVKLAVSGCPRNCAEVAIKDVGVIGVDSGWEIYVGGNGG
IKTEVAQFLVKVKTPDEVLEYSGAFLQLYREEARYLDRTVHYLERVGLDYVKKKVVDDAASRCALYERLLFALSVERDPW
LERAQEGKLKHEFETISA

Sequences:

>Translated_818_residues
MDMSTPMGQGPKMKLVMVGNGMAGVRTLEELLKLAPELYDITVFGAEPHPNYNRILLSPVLAGEQTVDDIILNGADWYSE
NGIRLHLGQKVVRIDRAAREVETADGTRARYDRLLLATGSTPFILPVAGADLPGVVSFRDIADVDAMVDAAGRYKHAVVI
GGGLLGLEAANGLMKRGMDVTVVHIGPWLMERQLDEPAARLLQRSLEEKGMKFLLQKHTAELVAGASGRVTGVRFKDGDA
IPADLVVMAVGIRPNTELAESAGLHCNRGIVVNDTMQTFDPRIYSIGECVAHRGVAYGLVAPLFEQAKVCANHLAHYGIG
RYQGSATSTKLKVTGVDLFSAGDFMGGEGTETILYSDPIGGVYKKLVIRDDKLVGGVMVGDTVDGAWYFSLLRDGKDIGA
LRDHLMFGQDHCGNLGHQGINRAAAMTDDMEVCGCNGICKGDIVKAIREKGLFTLEDVRKHTKASSSCGSCTGLVEQILA
VTAGGDYSAAPKTKSMCGCTDHTHEAVRAAIREHRLLTIPQAMAFLEWRTPNGCASCRPALNYYLISTWPGEAEDDPQSR
FINERAHANIQKDGSYSVIPRMWGGQTTPAELRRIAEVAEKYAVPSVKVTGGQRIDLLGVKKEDLPKIWADLDMPSGHAY
GKALRTVKTCVGSEWCRFGTQDSTQMGIDLEKVFYKMWAPHKVKLAVSGCPRNCAEVAIKDVGVIGVDSGWEIYVGGNGG
IKTEVAQFLVKVKTPDEVLEYSGAFLQLYREEARYLDRTVHYLERVGLDYVKKKVVDDAASRCALYERLLFALSVERDPW
LERAQEGKLKHEFETISA
>Mature_818_residues
MDMSTPMGQGPKMKLVMVGNGMAGVRTLEELLKLAPELYDITVFGAEPHPNYNRILLSPVLAGEQTVDDIILNGADWYSE
NGIRLHLGQKVVRIDRAAREVETADGTRARYDRLLLATGSTPFILPVAGADLPGVVSFRDIADVDAMVDAAGRYKHAVVI
GGGLLGLEAANGLMKRGMDVTVVHIGPWLMERQLDEPAARLLQRSLEEKGMKFLLQKHTAELVAGASGRVTGVRFKDGDA
IPADLVVMAVGIRPNTELAESAGLHCNRGIVVNDTMQTFDPRIYSIGECVAHRGVAYGLVAPLFEQAKVCANHLAHYGIG
RYQGSATSTKLKVTGVDLFSAGDFMGGEGTETILYSDPIGGVYKKLVIRDDKLVGGVMVGDTVDGAWYFSLLRDGKDIGA
LRDHLMFGQDHCGNLGHQGINRAAAMTDDMEVCGCNGICKGDIVKAIREKGLFTLEDVRKHTKASSSCGSCTGLVEQILA
VTAGGDYSAAPKTKSMCGCTDHTHEAVRAAIREHRLLTIPQAMAFLEWRTPNGCASCRPALNYYLISTWPGEAEDDPQSR
FINERAHANIQKDGSYSVIPRMWGGQTTPAELRRIAEVAEKYAVPSVKVTGGQRIDLLGVKKEDLPKIWADLDMPSGHAY
GKALRTVKTCVGSEWCRFGTQDSTQMGIDLEKVFYKMWAPHKVKLAVSGCPRNCAEVAIKDVGVIGVDSGWEIYVGGNGG
IKTEVAQFLVKVKTPDEVLEYSGAFLQLYREEARYLDRTVHYLERVGLDYVKKKVVDDAASRCALYERLLFALSVERDPW
LERAQEGKLKHEFETISA

Specific function: Required for nitrite assimilation [H]

COG id: COG1251

COG function: function code C; NAD(P)H-nitrite reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitrite and sulfite reductase 4Fe-4S domain family [H]

Homologues:

Organism=Homo sapiens, GI65787454, Length=292, Percent_Identity=29.4520547945205, Blast_Score=110, Evalue=5e-24,
Organism=Homo sapiens, GI226437568, Length=292, Percent_Identity=29.4520547945205, Blast_Score=110, Evalue=5e-24,
Organism=Homo sapiens, GI21389617, Length=292, Percent_Identity=29.4520547945205, Blast_Score=110, Evalue=5e-24,
Organism=Homo sapiens, GI22202629, Length=207, Percent_Identity=30.9178743961353, Blast_Score=82, Evalue=3e-15,
Organism=Homo sapiens, GI4757732, Length=207, Percent_Identity=30.9178743961353, Blast_Score=82, Evalue=3e-15,
Organism=Escherichia coli, GI1789765, Length=782, Percent_Identity=36.0613810741688, Blast_Score=447, Evalue=1e-126,
Organism=Escherichia coli, GI1789065, Length=303, Percent_Identity=30.03300330033, Blast_Score=117, Evalue=4e-27,
Organism=Escherichia coli, GI1788892, Length=254, Percent_Identity=28.3464566929134, Blast_Score=113, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17559934, Length=432, Percent_Identity=25.9259259259259, Blast_Score=125, Evalue=1e-28,
Organism=Drosophila melanogaster, GI281359715, Length=321, Percent_Identity=24.6105919003115, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI281359713, Length=321, Percent_Identity=24.6105919003115, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24639250, Length=321, Percent_Identity=24.6105919003115, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI18543267, Length=321, Percent_Identity=24.6105919003115, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24639252, Length=321, Percent_Identity=24.6105919003115, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI24639257, Length=321, Percent_Identity=24.6105919003115, Blast_Score=105, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24585130, Length=321, Percent_Identity=23.0529595015576, Blast_Score=99, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21358499, Length=218, Percent_Identity=32.1100917431193, Blast_Score=75, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007419
- InterPro:   IPR013027
- InterPro:   IPR005117
- InterPro:   IPR012744
- InterPro:   IPR017121
- InterPro:   IPR006067
- InterPro:   IPR006066
- InterPro:   IPR001327 [H]

Pfam domain/function: PF04324 Fer2_BFD; PF01077 NIR_SIR; PF03460 NIR_SIR_ferr; PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.7.1.4 [H]

Molecular weight: Translated: 89180; Mature: 89180

Theoretical pI: Translated: 6.62; Mature: 6.62

Prosite motif: PS00365 NIR_SIR ; PS00132 CARBOXYPEPT_ZN_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDMSTPMGQGPKMKLVMVGNGMAGVRTLEELLKLAPELYDITVFGAEPHPNYNRILLSPV
CCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEHH
LAGEQTVDDIILNGADWYSENGIRLHLGQKVVRIDRAAREVETADGTRARYDRLLLATGS
HCCCHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCHHHHCEEEEEECC
TPFILPVAGADLPGVVSFRDIADVDAMVDAAGRYKHAVVIGGGLLGLEAANGLMKRGMDV
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCE
TVVHIGPWLMERQLDEPAARLLQRSLEEKGMKFLLQKHTAELVAGASGRVTGVRFKDGDA
EEEEECHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
IPADLVVMAVGIRPNTELAESAGLHCNRGIVVNDTMQTFDPRIYSIGECVAHRGVAYGLV
CCCCEEEEEECCCCCCHHHHHCCCCCCCCEEECCCHHHHCCHHHHHHHHHHHCCHHHHHH
APLFEQAKVCANHLAHYGIGRYQGSATSTKLKVTGVDLFSAGDFMGGEGTETILYSDPIG
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCEEEEECCCC
GVYKKLVIRDDKLVGGVMVGDTVDGAWYFSLLRDGKDIGALRDHLMFGQDHCGNLGHQGI
HHHHHHHCCCCCEECCEEECCCCCHHHHHHHHHCCCHHHHHHHHHHCCCHHCCCCCHHHH
NRAAAMTDDMEVCGCNGICKGDIVKAIREKGLFTLEDVRKHTKASSSCGSCTGLVEQILA
HHHHHCCCCCHHCCCCCCCHHHHHHHHHHCCCEEHHHHHHHHHCCCCCCHHHHHHHHHHH
VTAGGDYSAAPKTKSMCGCTDHTHEAVRAAIREHRLLTIPQAMAFLEWRTPNGCASCRPA
HCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEECHHHHHHEEECCCCCCCCCCCC
LNYYLISTWPGEAEDDPQSRFINERAHANIQKDGSYSVIPRMWGGQTTPAELRRIAEVAE
CEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHH
KYAVPSVKVTGGQRIDLLGVKKEDLPKIWADLDMPSGHAYGKALRTVKTCVGSEWCRFGT
HHCCCCEEECCCCEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHCCC
QDSTQMGIDLEKVFYKMWAPHKVKLAVSGCPRNCAEVAIKDVGVIGVDSGWEIYVGGNGG
CCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCEEEECCCCEEEECCCCC
IKTEVAQFLVKVKTPDEVLEYSGAFLQLYREEARYLDRTVHYLERVGLDYVKKKVVDDAA
CHHHHHHHHEECCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
SRCALYERLLFALSVERDPWLERAQEGKLKHEFETISA
HHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHCCC
>Mature Secondary Structure
MDMSTPMGQGPKMKLVMVGNGMAGVRTLEELLKLAPELYDITVFGAEPHPNYNRILLSPV
CCCCCCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEEEEEHH
LAGEQTVDDIILNGADWYSENGIRLHLGQKVVRIDRAAREVETADGTRARYDRLLLATGS
HCCCHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCHHHHCEEEEEECC
TPFILPVAGADLPGVVSFRDIADVDAMVDAAGRYKHAVVIGGGLLGLEAANGLMKRGMDV
CCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHCCCCE
TVVHIGPWLMERQLDEPAARLLQRSLEEKGMKFLLQKHTAELVAGASGRVTGVRFKDGDA
EEEEECHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
IPADLVVMAVGIRPNTELAESAGLHCNRGIVVNDTMQTFDPRIYSIGECVAHRGVAYGLV
CCCCEEEEEECCCCCCHHHHHCCCCCCCCEEECCCHHHHCCHHHHHHHHHHHCCHHHHHH
APLFEQAKVCANHLAHYGIGRYQGSATSTKLKVTGVDLFSAGDFMGGEGTETILYSDPIG
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCEEEEECCCC
GVYKKLVIRDDKLVGGVMVGDTVDGAWYFSLLRDGKDIGALRDHLMFGQDHCGNLGHQGI
HHHHHHHCCCCCEECCEEECCCCCHHHHHHHHHCCCHHHHHHHHHHCCCHHCCCCCHHHH
NRAAAMTDDMEVCGCNGICKGDIVKAIREKGLFTLEDVRKHTKASSSCGSCTGLVEQILA
HHHHHCCCCCHHCCCCCCCHHHHHHHHHHCCCEEHHHHHHHHHCCCCCCHHHHHHHHHHH
VTAGGDYSAAPKTKSMCGCTDHTHEAVRAAIREHRLLTIPQAMAFLEWRTPNGCASCRPA
HCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEECHHHHHHEEECCCCCCCCCCCC
LNYYLISTWPGEAEDDPQSRFINERAHANIQKDGSYSVIPRMWGGQTTPAELRRIAEVAE
CEEEEEEECCCCCCCCHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCHHHHHHHHHHHH
KYAVPSVKVTGGQRIDLLGVKKEDLPKIWADLDMPSGHAYGKALRTVKTCVGSEWCRFGT
HHCCCCEEECCCCEEEEECCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHHHCCC
QDSTQMGIDLEKVFYKMWAPHKVKLAVSGCPRNCAEVAIKDVGVIGVDSGWEIYVGGNGG
CCCCCCCCCHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCEEEECCCCEEEECCCCC
IKTEVAQFLVKVKTPDEVLEYSGAFLQLYREEARYLDRTVHYLERVGLDYVKKKVVDDAA
CHHHHHHHHEECCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
SRCALYERLLFALSVERDPWLERAQEGKLKHEFETISA
HHHHHHHHHHHHHCCCCCHHHHHHHCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7868621; 8969502; 9384377 [H]