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The map label for this gene is cls [H]

Identifier: 74318261

GI number: 74318261

Start: 2317918

End: 2319360

Strand: Direct

Name: cls [H]

Synonym: Tbd_2243

Alternate gene names: 74318261

Gene position: 2317918-2319360 (Clockwise)

Preceding gene: 74318260

Following gene: 74318262

Centisome position: 79.66

GC content: 66.81

Gene sequence:

>1443_bases
ATGCCGCATCCGAGCCCCTCACCCATCCCGCGACGCAGTCGCGGCGGGATCGCGGGCCTGACAAGAATCCTGATCGCTTG
TTGCGCGGCGCCGCTCTGCGCCTGCGCCACCCTGCCCGAGGCGCCGCGTCTCGCGGCGGGTCCAGTCGCCAGTCCGCCGG
TCAATTTCGAGTCCGCTCGCGGCCCGGTTTCGGCACGCCGCAACGCGGCGATCTTCGCCGAGCTCAAGCGCGACGGCGGC
GACAGCGCGATCCTGCTGCGCCACCTCGCGCTCGAGGAAGCGGTCAATCGCGACAGCCCGCTCGTCGTCGGCAACCGGCT
GACGTTGCTGCAGGACGGGCCGGCGACCTACCGCGCGATGTTCGCCGCGATCCGCAGCGCTCGCGATCACGTCAACCTGC
AGACCTATATTTTCGAGGCGGGGCAGACCGGCGAGACCTTCGCGCGCCTGCTGCTCGAGAAACAGGCTGCCGGCGTGCAG
GTCAACATCATCTACGACAGTGTCGGCTCGATCGACACGCCGCCCGCTTTCTTCCAGCGGCTGCGCGACGCCGGCATGCG
CGTGCTCGAATTCAATCCGGTCAACCCGGCCAAGCTCAAGAAGAAGAAATGGCGGCTCAACAACCGCGATCACCGGAAGC
TGCTCGTCGTCGACGGCAAGACGGCTTTTCTCGGCGGGATCAACATCAGCGAGACCTATTCGAGCGGCTCGCTGTCGCGC
CCGAGCAAGAAAAAAGCCAAGGACCGTGAATCGGAGGGATGGCGCGACACGCATCTGCAGGTCGACGGTCCTGCCGTCGC
CGAATTCCAGAAGCTGTTTCTCGACACCTGGGCGCGGCAGAAAGGCCCGCCGCTGCCCGCGCGCGCGTATTTCCCCAAGC
TCGCGCGCCAAGGCAACGAAATCGTGCGCGCGATCGCGAGCGAAGCCGACGAGGGCGACAGCCCGATCTATCTGACGCTG
CTCTCGGCGATCGAACACGCCGAGCGCAGCATCCGCCTGACCAACGCGTATTTCGTCCCCGACCCGCAATTGCGCCGGGC
GCTCGCCGCCGCGTCCCGGCGCGGCGTCGACGTGCAGATCATCCTGCCGAGCCGGACCGACTCGTGGGCCGTGTTCCACG
CCGGACGCTCGCACTACGACGAACTGCTCGACGCCGGCGTGAAGATCTACGAACGCCACGGTACCGTGCTGCACGCCAAG
ACCGCGTCGGTCGACGGCGTCTGGTCGACGATCGGTTCGACCAACCTCGACTGGCGCAGCTTTCTTCACAACGACGAGCT
CAACGCGGTCGTGCTCGGAACCGACTTCGCCCGCCAGCTCGACGCGATGTTCGTGCGCGACCGGGCGGCATCGGAAAAGA
TCGACCTCGAGAAATGGCGTCGTCGCGCGCTGCACATCCGCATGCAGGAATGGATCGCCCGGCGCTTCGAGTACTGGCTG
TAG

Upstream 100 bases:

>100_bases
CGCTGTCGTCGGGTCGCAGCTGGCTGGAACGGCTCAACGCGGCGGCCGCCGCCGAGGACAGCAGTCCACCGCCGTAATCG
CGTGAGGGCGGGGATACGCG

Downstream 100 bases:

>100_bases
ATCGCGAGCGCCTCGGAGAACGCGGGAACGGAACTTAATCGCCAACGCGGCGACGAATTCGAGTCGCGCCGAATTACAAG
CGGCCCCTTCCGGGCCAGAA

Product: cardiolipin synthetase 2

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 480; Mature: 479

Protein sequence:

>480_residues
MPHPSPSPIPRRSRGGIAGLTRILIACCAAPLCACATLPEAPRLAAGPVASPPVNFESARGPVSARRNAAIFAELKRDGG
DSAILLRHLALEEAVNRDSPLVVGNRLTLLQDGPATYRAMFAAIRSARDHVNLQTYIFEAGQTGETFARLLLEKQAAGVQ
VNIIYDSVGSIDTPPAFFQRLRDAGMRVLEFNPVNPAKLKKKKWRLNNRDHRKLLVVDGKTAFLGGINISETYSSGSLSR
PSKKKAKDRESEGWRDTHLQVDGPAVAEFQKLFLDTWARQKGPPLPARAYFPKLARQGNEIVRAIASEADEGDSPIYLTL
LSAIEHAERSIRLTNAYFVPDPQLRRALAAASRRGVDVQIILPSRTDSWAVFHAGRSHYDELLDAGVKIYERHGTVLHAK
TASVDGVWSTIGSTNLDWRSFLHNDELNAVVLGTDFARQLDAMFVRDRAASEKIDLEKWRRRALHIRMQEWIARRFEYWL

Sequences:

>Translated_480_residues
MPHPSPSPIPRRSRGGIAGLTRILIACCAAPLCACATLPEAPRLAAGPVASPPVNFESARGPVSARRNAAIFAELKRDGG
DSAILLRHLALEEAVNRDSPLVVGNRLTLLQDGPATYRAMFAAIRSARDHVNLQTYIFEAGQTGETFARLLLEKQAAGVQ
VNIIYDSVGSIDTPPAFFQRLRDAGMRVLEFNPVNPAKLKKKKWRLNNRDHRKLLVVDGKTAFLGGINISETYSSGSLSR
PSKKKAKDRESEGWRDTHLQVDGPAVAEFQKLFLDTWARQKGPPLPARAYFPKLARQGNEIVRAIASEADEGDSPIYLTL
LSAIEHAERSIRLTNAYFVPDPQLRRALAAASRRGVDVQIILPSRTDSWAVFHAGRSHYDELLDAGVKIYERHGTVLHAK
TASVDGVWSTIGSTNLDWRSFLHNDELNAVVLGTDFARQLDAMFVRDRAASEKIDLEKWRRRALHIRMQEWIARRFEYWL
>Mature_479_residues
PHPSPSPIPRRSRGGIAGLTRILIACCAAPLCACATLPEAPRLAAGPVASPPVNFESARGPVSARRNAAIFAELKRDGGD
SAILLRHLALEEAVNRDSPLVVGNRLTLLQDGPATYRAMFAAIRSARDHVNLQTYIFEAGQTGETFARLLLEKQAAGVQV
NIIYDSVGSIDTPPAFFQRLRDAGMRVLEFNPVNPAKLKKKKWRLNNRDHRKLLVVDGKTAFLGGINISETYSSGSLSRP
SKKKAKDRESEGWRDTHLQVDGPAVAEFQKLFLDTWARQKGPPLPARAYFPKLARQGNEIVRAIASEADEGDSPIYLTLL
SAIEHAERSIRLTNAYFVPDPQLRRALAAASRRGVDVQIILPSRTDSWAVFHAGRSHYDELLDAGVKIYERHGTVLHAKT
ASVDGVWSTIGSTNLDWRSFLHNDELNAVVLGTDFARQLDAMFVRDRAASEKIDLEKWRRRALHIRMQEWIARRFEYWL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=409, Percent_Identity=30.5623471882641, Blast_Score=164, Evalue=1e-41,
Organism=Escherichia coli, GI1787007, Length=343, Percent_Identity=28.5714285714286, Blast_Score=131, Evalue=8e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR015679
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 53387; Mature: 53255

Theoretical pI: Translated: 10.22; Mature: 10.22

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHPSPSPIPRRSRGGIAGLTRILIACCAAPLCACATLPEAPRLAAGPVASPPVNFESAR
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
GPVSARRNAAIFAELKRDGGDSAILLRHLALEEAVNRDSPLVVGNRLTLLQDGPATYRAM
CCCHHCCCHHEEEEEHHCCCCHHHHHHHHHHHHHHCCCCCEEECCEEEEEECCCHHHHHH
FAAIRSARDHVNLQTYIFEAGQTGETFARLLLEKQAAGVQVNIIYDSVGSIDTPPAFFQR
HHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCHHHHHH
LRDAGMRVLEFNPVNPAKLKKKKWRLNNRDHRKLLVVDGKTAFLGGINISETYSSGSLSR
HHHCCCEEEEECCCCHHHHHHHHHCCCCCCCCEEEEECCCEEEEECCCCCCCCCCCCCCC
PSKKKAKDRESEGWRDTHLQVDGPAVAEFQKLFLDTWARQKGPPLPARAYFPKLARQGNE
CCHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
IVRAIASEADEGDSPIYLTLLSAIEHAERSIRLTNAYFVPDPQLRRALAAASRRGVDVQI
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHCEEEEEEEECCCHHHHHHHHHHHCCCCEEEE
ILPSRTDSWAVFHAGRSHYDELLDAGVKIYERHGTVLHAKTASVDGVWSTIGSTNLDWRS
EECCCCCCEEEEECCHHHHHHHHHHHHHEEECCCCEEEEECCCCCHHHHHHCCCCCHHHH
FLHNDELNAVVLGTDFARQLDAMFVRDRAASEKIDLEKWRRRALHIRMQEWIARRFEYWL
HHCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
PHPSPSPIPRRSRGGIAGLTRILIACCAAPLCACATLPEAPRLAAGPVASPPVNFESAR
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCC
GPVSARRNAAIFAELKRDGGDSAILLRHLALEEAVNRDSPLVVGNRLTLLQDGPATYRAM
CCCHHCCCHHEEEEEHHCCCCHHHHHHHHHHHHHHCCCCCEEECCEEEEEECCCHHHHHH
FAAIRSARDHVNLQTYIFEAGQTGETFARLLLEKQAAGVQVNIIYDSVGSIDTPPAFFQR
HHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHCCEEEEEEEECCCCCCCCHHHHHH
LRDAGMRVLEFNPVNPAKLKKKKWRLNNRDHRKLLVVDGKTAFLGGINISETYSSGSLSR
HHHCCCEEEEECCCCHHHHHHHHHCCCCCCCCEEEEECCCEEEEECCCCCCCCCCCCCCC
PSKKKAKDRESEGWRDTHLQVDGPAVAEFQKLFLDTWARQKGPPLPARAYFPKLARQGNE
CCHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
IVRAIASEADEGDSPIYLTLLSAIEHAERSIRLTNAYFVPDPQLRRALAAASRRGVDVQI
HHHHHHHCCCCCCCCCHHHHHHHHHHHHHCEEEEEEEECCCHHHHHHHHHHHCCCCEEEE
ILPSRTDSWAVFHAGRSHYDELLDAGVKIYERHGTVLHAKTASVDGVWSTIGSTNLDWRS
EECCCCCCEEEEECCHHHHHHHHHHHHHEEECCCCEEEEECCCCCHHHHHHCCCCCHHHH
FLHNDELNAVVLGTDFARQLDAMFVRDRAASEKIDLEKWRRRALHIRMQEWIARRFEYWL
HHCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA