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The map label for this gene is thiG

Identifier: 74318144

GI number: 74318144

Start: 2213861

End: 2214649

Strand: Reverse

Name: thiG

Synonym: Tbd_2126

Alternate gene names: 74318144

Gene position: 2214649-2213861 (Counterclockwise)

Preceding gene: 74318145

Following gene: 74318143

Centisome position: 76.11

GC content: 64.89

Gene sequence:

>789_bases
ATGCAACCTCTCATCATCGCCGGACGATCCTATGCCTCGCGCCTGCTGGTCGGCACCGGGAAATACCAGGACTTCGAGCA
GACCCGGCTTGCGGTCGAAGCGTCAGGGGCCGAGATCGTCACGGTCGCCATCCGCCGCACCAACATCGGCCAGGACGCCG
GTCAGCCCAGCCTGCTCGACGCGCTGCCGCCGTCTAAATACACCTACCTGCCGAATACAGCCGGCTGTTATACGGCAGAG
GACGCGATCCGCACGCTGCGTCTGGCGCGCGAACTGCTCGATGGCCATGCCCTGGTCAAGCTCGAGGTGCTGGGCGACGC
CGAGTCGCTGTACCCCAACGTTGCCGAGACGATCAAGGCGGCCGAAGTGCTCGTCAAGGAGGGTTTCCAGGTCATGGTTT
ACACCTCGGATGATCCGATCGCCGCCAAGCAGCTCGAAGACATCGGCTGCGTCGCGGTGATGCCGCTCGCGAGCCTGATC
GGTTCGGGCATGGGCATCCTCAATCCGTGGAATCTGCAGATCATCATCGACCGCCTCACGGTCCCCGTGATCGTGGACGC
GGGCGTCGGTACCGCGAGCGACGCAACGATCGCGATGGAACTCGGCTGCGACGCCGTGCTCATGAACACGGCGATCGCAG
GGGCCAGCGATCCGGTGCTGATGGCGTCGGCGATGAAAAAGGCCGTCGAAGCCGGTCGCGAGGCCTTCCTCGCCGGCCGG
ATGCCGAAAAAGATCTATCGCGCCAGCCCGAGCTCGCCGACCAGCGGCCTCATCAGCGGCAGCAAGTGA

Upstream 100 bases:

>100_bases
ACACTGCGCTTGCCGACGGCGACCGCCTCGAGATCGTCGTCGCGGTGGGTGGCGGCTGAACAGATCCTCTCCAGCGTTTT
TCTGCACCGACTCCGAAATC

Downstream 100 bases:

>100_bases
GCAGCATGACAGCAGACGGCGGCGCTCGCCCGCGCATCCGCTCGTACGTGCTGCGGGCGGGTCGCGTCGGCTCGGGGCAG
GCCCGCGCGCTCGTCGAACT

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 262; Mature: 262

Protein sequence:

>262_residues
MQPLIIAGRSYASRLLVGTGKYQDFEQTRLAVEASGAEIVTVAIRRTNIGQDAGQPSLLDALPPSKYTYLPNTAGCYTAE
DAIRTLRLARELLDGHALVKLEVLGDAESLYPNVAETIKAAEVLVKEGFQVMVYTSDDPIAAKQLEDIGCVAVMPLASLI
GSGMGILNPWNLQIIIDRLTVPVIVDAGVGTASDATIAMELGCDAVLMNTAIAGASDPVLMASAMKKAVEAGREAFLAGR
MPKKIYRASPSSPTSGLISGSK

Sequences:

>Translated_262_residues
MQPLIIAGRSYASRLLVGTGKYQDFEQTRLAVEASGAEIVTVAIRRTNIGQDAGQPSLLDALPPSKYTYLPNTAGCYTAE
DAIRTLRLARELLDGHALVKLEVLGDAESLYPNVAETIKAAEVLVKEGFQVMVYTSDDPIAAKQLEDIGCVAVMPLASLI
GSGMGILNPWNLQIIIDRLTVPVIVDAGVGTASDATIAMELGCDAVLMNTAIAGASDPVLMASAMKKAVEAGREAFLAGR
MPKKIYRASPSSPTSGLISGSK
>Mature_262_residues
MQPLIIAGRSYASRLLVGTGKYQDFEQTRLAVEASGAEIVTVAIRRTNIGQDAGQPSLLDALPPSKYTYLPNTAGCYTAE
DAIRTLRLARELLDGHALVKLEVLGDAESLYPNVAETIKAAEVLVKEGFQVMVYTSDDPIAAKQLEDIGCVAVMPLASLI
GSGMGILNPWNLQIIIDRLTVPVIVDAGVGTASDATIAMELGCDAVLMNTAIAGASDPVLMASAMKKAVEAGREAFLAGR
MPKKIYRASPSSPTSGLISGSK

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=258, Percent_Identity=46.1240310077519, Blast_Score=201, Evalue=3e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_THIDA (Q3SH10)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315884.1
- ProteinModelPortal:   Q3SH10
- SMR:   Q3SH10
- STRING:   Q3SH10
- GeneID:   3672898
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_2126
- NMPDR:   fig|292415.3.peg.2322
- eggNOG:   COG2022
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- PhylomeDB:   Q3SH10
- ProtClustDB:   PRK00208
- BioCyc:   TDEN292415:TBD_2126-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 27550; Mature: 27550

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: ACT_SITE 100-100 BINDING 161-161

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPLIIAGRSYASRLLVGTGKYQDFEQTRLAVEASGAEIVTVAIRRTNIGQDAGQPSLLD
CCCEEEECCHHHHEEEEECCCCCCHHHHEEEEEECCCEEEEEEEEECCCCCCCCCCCHHH
ALPPSKYTYLPNTAGCYTAEDAIRTLRLARELLDGHALVKLEVLGDAESLYPNVAETIKA
CCCCCCEEECCCCCCCEEHHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHCCHHHHHHHH
AEVLVKEGFQVMVYTSDDPIAAKQLEDIGCVAVMPLASLIGSGMGILNPWNLQIIIDRLT
HHHHHHCCCEEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECC
VPVIVDAGVGTASDATIAMELGCDAVLMNTAIAGASDPVLMASAMKKAVEAGREAFLAGR
EEEEEECCCCCCCCCEEEEECCCCEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCC
MPKKIYRASPSSPTSGLISGSK
CCHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MQPLIIAGRSYASRLLVGTGKYQDFEQTRLAVEASGAEIVTVAIRRTNIGQDAGQPSLLD
CCCEEEECCHHHHEEEEECCCCCCHHHHEEEEEECCCEEEEEEEEECCCCCCCCCCCHHH
ALPPSKYTYLPNTAGCYTAEDAIRTLRLARELLDGHALVKLEVLGDAESLYPNVAETIKA
CCCCCCEEECCCCCCCEEHHHHHHHHHHHHHHHCCCEEEEEEEECCHHHHCCHHHHHHHH
AEVLVKEGFQVMVYTSDDPIAAKQLEDIGCVAVMPLASLIGSGMGILNPWNLQIIIDRLT
HHHHHHCCCEEEEEECCCCHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCCEEEEEEECC
VPVIVDAGVGTASDATIAMELGCDAVLMNTAIAGASDPVLMASAMKKAVEAGREAFLAGR
EEEEEECCCCCCCCCEEEEECCCCEEEHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCC
MPKKIYRASPSSPTSGLISGSK
CCHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA