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The map label for this gene is trmB

Identifier: 74318143

GI number: 74318143

Start: 2213172

End: 2213855

Strand: Reverse

Name: trmB

Synonym: Tbd_2125

Alternate gene names: 74318143

Gene position: 2213855-2213172 (Counterclockwise)

Preceding gene: 74318144

Following gene: 74318142

Centisome position: 76.08

GC content: 68.86

Gene sequence:

>684_bases
ATGACAGCAGACGGCGGCGCTCGCCCGCGCATCCGCTCGTACGTGCTGCGGGCGGGTCGCGTCGGCTCGGGGCAGGCCCG
CGCGCTCGTCGAACTCGGCCCGCGCTTCGTACTGCCCTACCAGCCCGAGCTGCTCGATCTGGACGCCGTGTTCGCTCGCG
TCGCGCCCCGCGTTCTGGAGATCGGCTTCGGCATGGGCGAAGGCTTGGCGGAAACGGCCGCGTCCCATCCGGAAATCGAC
TATATCGGCGTCGAGGTGCACACGCCCGGCGTCGGCGCCTTGCTCAAGCAGCTTGGCGAGCGCGAACTCGGCAACGTTCG
CGTGATCCAGCACGACGCCGTCGAGGTGCTGACCGCGATGCTCGCGCCCGCGACGCTCGCCGGCATCCATATCTTCTTTC
CCGATCCCTGGCACAAGAAGCGCCATCACAAGCGCCGCCTGATCCAGGCCCCCCTGGTCGAGTTGCTCGCGAGCCGCCTC
GCGCCGGGGGGCTACATCCATCTTGCAACCGACTGGCAGGACTACGCCGAGCAGATGCTCGTCGTGCTCGAGGCGGAGGC
CCAGCTGGAAAATACCGCCGCCGCTTACGTGCCTCGTCCCGACACCCGCCCGCTCACCAAATTCGAGCAGCGCGGGATCC
GGCTCGGCCACGGCGTCTGGGACCTGGTTTTCCGCAGGCGCTGA

Upstream 100 bases:

>100_bases
GTCGCGAGGCCTTCCTCGCCGGCCGGATGCCGAAAAAGATCTATCGCGCCAGCCCGAGCTCGCCGACCAGCGGCCTCATC
AGCGGCAGCAAGTGAGCAGC

Downstream 100 bases:

>100_bases
AGCACGACATGTCATGTGGCTATGATGCATGACATGGACCTCCTCGAACTGCTGAAGGCGGCGCTCGCGCTGTTCGCGAT
CGTCGATCCCGTCGGTGTCA

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 227; Mature: 226

Protein sequence:

>227_residues
MTADGGARPRIRSYVLRAGRVGSGQARALVELGPRFVLPYQPELLDLDAVFARVAPRVLEIGFGMGEGLAETAASHPEID
YIGVEVHTPGVGALLKQLGERELGNVRVIQHDAVEVLTAMLAPATLAGIHIFFPDPWHKKRHHKRRLIQAPLVELLASRL
APGGYIHLATDWQDYAEQMLVVLEAEAQLENTAAAYVPRPDTRPLTKFEQRGIRLGHGVWDLVFRRR

Sequences:

>Translated_227_residues
MTADGGARPRIRSYVLRAGRVGSGQARALVELGPRFVLPYQPELLDLDAVFARVAPRVLEIGFGMGEGLAETAASHPEID
YIGVEVHTPGVGALLKQLGERELGNVRVIQHDAVEVLTAMLAPATLAGIHIFFPDPWHKKRHHKRRLIQAPLVELLASRL
APGGYIHLATDWQDYAEQMLVVLEAEAQLENTAAAYVPRPDTRPLTKFEQRGIRLGHGVWDLVFRRR
>Mature_226_residues
TADGGARPRIRSYVLRAGRVGSGQARALVELGPRFVLPYQPELLDLDAVFARVAPRVLEIGFGMGEGLAETAASHPEIDY
IGVEVHTPGVGALLKQLGERELGNVRVIQHDAVEVLTAMLAPATLAGIHIFFPDPWHKKRHHKRRLIQAPLVELLASRLA
PGGYIHLATDWQDYAEQMLVVLEAEAQLENTAAAYVPRPDTRPLTKFEQRGIRLGHGVWDLVFRRR

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=227, Percent_Identity=51.9823788546256, Blast_Score=237, Evalue=4e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_THIDA (Q3SH11)

Other databases:

- EMBL:   CP000116
- RefSeq:   YP_315883.1
- ProteinModelPortal:   Q3SH11
- SMR:   Q3SH11
- STRING:   Q3SH11
- GeneID:   3672897
- GenomeReviews:   CP000116_GR
- KEGG:   tbd:Tbd_2125
- NMPDR:   fig|292415.3.peg.2321
- eggNOG:   COG0220
- HOGENOM:   HBG322782
- OMA:   YAEWIDE
- PhylomeDB:   Q3SH11
- BioCyc:   TDEN292415:TBD_2125-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 25135; Mature: 25004

Theoretical pI: Translated: 8.86; Mature: 8.86

Prosite motif: NA

Important sites: BINDING 60-60 BINDING 85-85 BINDING 112-112 BINDING 135-135 BINDING 139-139 BINDING 171-171

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTADGGARPRIRSYVLRAGRVGSGQARALVELGPRFVLPYQPELLDLDAVFARVAPRVLE
CCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHH
IGFGMGEGLAETAASHPEIDYIGVEVHTPGVGALLKQLGERELGNVRVIQHDAVEVLTAM
HCCCCCCCHHHHHCCCCCCEEEEEEEECCCHHHHHHHHCCCCCCCEEEEHHHHHHHHHHH
LAPATLAGIHIFFPDPWHKKRHHKRRLIQAPLVELLASRLAPGGYIHLATDWQDYAEQML
HHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHH
VVLEAEAQLENTAAAYVPRPDTRPLTKFEQRGIRLGHGVWDLVFRRR
HHHHHHHHHCCCEEEECCCCCCCCHHHHHHCCCCCCCHHHHHHHCCC
>Mature Secondary Structure 
TADGGARPRIRSYVLRAGRVGSGQARALVELGPRFVLPYQPELLDLDAVFARVAPRVLE
CCCCCCCHHHHHHHHHCCCCCCCHHHHHHHCCCEEECCCCCCCCCHHHHHHHHHHHHHH
IGFGMGEGLAETAASHPEIDYIGVEVHTPGVGALLKQLGERELGNVRVIQHDAVEVLTAM
HCCCCCCCHHHHHCCCCCCEEEEEEEECCCHHHHHHHHCCCCCCCEEEEHHHHHHHHHHH
LAPATLAGIHIFFPDPWHKKRHHKRRLIQAPLVELLASRLAPGGYIHLATDWQDYAEQML
HHHHHHHEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHH
VVLEAEAQLENTAAAYVPRPDTRPLTKFEQRGIRLGHGVWDLVFRRR
HHHHHHHHHCCCEEEECCCCCCCCHHHHHHCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA