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The map label for this gene is dnaB [H]

Identifier: 74318119

GI number: 74318119

Start: 2191379

End: 2192770

Strand: Reverse

Name: dnaB [H]

Synonym: Tbd_2101

Alternate gene names: 74318119

Gene position: 2192770-2191379 (Counterclockwise)

Preceding gene: 74318120

Following gene: 74318118

Centisome position: 75.36

GC content: 66.52

Gene sequence:

>1392_bases
ATGGCAGCCATCCACTCCCTGACGTCGACGTCCGACCCCCAGCTCGCGCAGATGCGCCTGCCGCCGCATTCGCTCGAGGC
CGAGCAGGCCGTCCTGGGGGGGCTGCTGCTCGACAACGGCGCGTGGGACCGCATCGGCGATGTCGCCTCGGAGAGCGACT
TCTACCGTCAGGATCATCGCCAGATCCTGCGCGCGATCGTGCAGCAGATCGCCGAAAACCGGCCGGCGGACGCGGTGACC
GTGTCCGAAGCCCTGCAGTCGATGGGCCAGCTCGAAAGCGTCGGCGGCCTCGCCTACATCGTCGCACTGGCGAGCAACAC
GCCGTCGGCCGCCAACATCCGGCGCTACGCGGAGATCGTGCGTGAACGAGCGGTCATGCGGCGCTTGGCCGAGGTCGCGA
CCGGCATCGCGGACACGGCGTACGCGCCGGCCGGGCGCAGCGCTTCGCAATTGCTCGACGAAGCCGAAGCCAAGGTGTTC
GAGATCGCCGAATCGGGCAGCCGCGGACAGGCCGGGTTCACCGAAATCAAGCCTCTGCTCAAGGAAGTCGTCGAGCGCAT
CGACGAGCTCTATCACCGCGACAACGATTCTGGCATCACTGGCGTTCCGACCGGGTTCCACGATCTCGACCAGAAGACCT
CGGGCCTGCAGCCCGGCGATCTCGTCATCGTCGCGGGCCGGCCGTCGATGGGCAAAACCGCGTTCTCGATCAACATCGCC
GAGAACGTCGCGCTCGACACCGGGCTTCCGGTCGCGGTGTTCTCGATGGAAATGGGCGGCACCCAACTGGTGATGCGGAT
GATCGGATCGGTCGGCAGGCTCGACCAGCACCGCCTGCGCACGGGCAAGCTCGGCGAGGACGACTGGCAGCGCCTGACCT
ATGCGCTCGGCAAGCTCAACGAAGCGCCGGTCTACATCGACGAAAGTCCGGGCCTCAACGTGCTCGAACTGCGTGCCCGC
GCGCGGCGCCTGATGCGCCAGTGCGGCAAGCTCGGGCTCATCGTGATCGACTACCTGCAGCTCATGGCCGGCAGCGGCGG
CGGCGAGAACCGCGCCACTGAAATTTCCGAAATCTCGCGTGCGCTCAAGGGGCTGGCGAAGGAACTGCACGTACCGGTGA
TCGCGCTGTCGCAGCTGAACCGCGGCCTTGAGCAGCGCCCGAACAAGCGCCCGGTGATGTCCGACCTGCGGGAGTCGGGC
GCCATCGAGCAGGACGCCGACGTCATCCTGTTCATCTACCGCGACGAGGTCTACAACCCCGATACGCAGGACAAGGGTAC
GGCCGAAATCATCATCAGCAAGCAGCGTAACGGCCCGATCGGCACGGTGCGGCTGGCTTTCCTCGGCGAGTACACGCGCT
TCGAAACGCTGGCGCAGCCGGGCTCGTACTAA

Upstream 100 bases:

>100_bases
TTTTTGCGCCTTCCCGCGGCGGCTTTATTCCGGTTCATACACGCTTTATCCACAGTCTTATCCCTTGGTCGGCCCGGAGC
CGGAGACTAGAATCGGCCCC

Downstream 100 bases:

>100_bases
CCCTTCCGATGCGTCCGATCAAAGCGTCGATCTCGGCGGACGCGATGGCCCACAACCTTCGCGTCGCACGGCGTCACGCC
GGCGCCGCGCGGGTGTTCGC

Product: primary replicative DNA helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 463; Mature: 462

Protein sequence:

>463_residues
MAAIHSLTSTSDPQLAQMRLPPHSLEAEQAVLGGLLLDNGAWDRIGDVASESDFYRQDHRQILRAIVQQIAENRPADAVT
VSEALQSMGQLESVGGLAYIVALASNTPSAANIRRYAEIVRERAVMRRLAEVATGIADTAYAPAGRSASQLLDEAEAKVF
EIAESGSRGQAGFTEIKPLLKEVVERIDELYHRDNDSGITGVPTGFHDLDQKTSGLQPGDLVIVAGRPSMGKTAFSINIA
ENVALDTGLPVAVFSMEMGGTQLVMRMIGSVGRLDQHRLRTGKLGEDDWQRLTYALGKLNEAPVYIDESPGLNVLELRAR
ARRLMRQCGKLGLIVIDYLQLMAGSGGGENRATEISEISRALKGLAKELHVPVIALSQLNRGLEQRPNKRPVMSDLRESG
AIEQDADVILFIYRDEVYNPDTQDKGTAEIIISKQRNGPIGTVRLAFLGEYTRFETLAQPGSY

Sequences:

>Translated_463_residues
MAAIHSLTSTSDPQLAQMRLPPHSLEAEQAVLGGLLLDNGAWDRIGDVASESDFYRQDHRQILRAIVQQIAENRPADAVT
VSEALQSMGQLESVGGLAYIVALASNTPSAANIRRYAEIVRERAVMRRLAEVATGIADTAYAPAGRSASQLLDEAEAKVF
EIAESGSRGQAGFTEIKPLLKEVVERIDELYHRDNDSGITGVPTGFHDLDQKTSGLQPGDLVIVAGRPSMGKTAFSINIA
ENVALDTGLPVAVFSMEMGGTQLVMRMIGSVGRLDQHRLRTGKLGEDDWQRLTYALGKLNEAPVYIDESPGLNVLELRAR
ARRLMRQCGKLGLIVIDYLQLMAGSGGGENRATEISEISRALKGLAKELHVPVIALSQLNRGLEQRPNKRPVMSDLRESG
AIEQDADVILFIYRDEVYNPDTQDKGTAEIIISKQRNGPIGTVRLAFLGEYTRFETLAQPGSY
>Mature_462_residues
AAIHSLTSTSDPQLAQMRLPPHSLEAEQAVLGGLLLDNGAWDRIGDVASESDFYRQDHRQILRAIVQQIAENRPADAVTV
SEALQSMGQLESVGGLAYIVALASNTPSAANIRRYAEIVRERAVMRRLAEVATGIADTAYAPAGRSASQLLDEAEAKVFE
IAESGSRGQAGFTEIKPLLKEVVERIDELYHRDNDSGITGVPTGFHDLDQKTSGLQPGDLVIVAGRPSMGKTAFSINIAE
NVALDTGLPVAVFSMEMGGTQLVMRMIGSVGRLDQHRLRTGKLGEDDWQRLTYALGKLNEAPVYIDESPGLNVLELRARA
RRLMRQCGKLGLIVIDYLQLMAGSGGGENRATEISEISRALKGLAKELHVPVIALSQLNRGLEQRPNKRPVMSDLRESGA
IEQDADVILFIYRDEVYNPDTQDKGTAEIIISKQRNGPIGTVRLAFLGEYTRFETLAQPGSY

Specific function: Participates in initiation and elongation during chromosome replication; it exhibits DNA-dependent ATPase activity and contains distinct active sites for ATP binding, DNA binding, and interaction with dnaC protein, primase, and other prepriming proteins [

COG id: COG0305

COG function: function code L; Replicative DNA helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 SF4 helicase domain [H]

Homologues:

Organism=Escherichia coli, GI1790486, Length=450, Percent_Identity=54.4444444444444, Blast_Score=497, Evalue=1e-142,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR007692
- InterPro:   IPR007694
- InterPro:   IPR007693
- InterPro:   IPR016136 [H]

Pfam domain/function: PF00772 DnaB; PF03796 DnaB_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 50413; Mature: 50282

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAIHSLTSTSDPQLAQMRLPPHSLEAEQAVLGGLLLDNGAWDRIGDVASESDFYRQDHR
CCCCCCCCCCCCCCHHEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHH
QILRAIVQQIAENRPADAVTVSEALQSMGQLESVGGLAYIVALASNTPSAANIRRYAEIV
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
RERAVMRRLAEVATGIADTAYAPAGRSASQLLDEAEAKVFEIAESGSRGQAGFTEIKPLL
HHHHHHHHHHHHHHCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
KEVVERIDELYHRDNDSGITGVPTGFHDLDQKTSGLQPGDLVIVAGRPSMGKTAFSINIA
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEEEH
ENVALDTGLPVAVFSMEMGGTQLVMRMIGSVGRLDQHRLRTGKLGEDDWQRLTYALGKLN
HHHECCCCCCEEEEEECCCHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHCCC
EAPVYIDESPGLNVLELRARARRLMRQCGKLGLIVIDYLQLMAGSGGGENRATEISEISR
CCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHH
ALKGLAKELHVPVIALSQLNRGLEQRPNKRPVMSDLRESGAIEQDADVILFIYRDEVYNP
HHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCC
DTQDKGTAEIIISKQRNGPIGTVRLAFLGEYTRFETLAQPGSY
CCCCCCCEEEEEEECCCCCCCEEEHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
AAIHSLTSTSDPQLAQMRLPPHSLEAEQAVLGGLLLDNGAWDRIGDVASESDFYRQDHR
CCCCCCCCCCCCCHHEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHH
QILRAIVQQIAENRPADAVTVSEALQSMGQLESVGGLAYIVALASNTPSAANIRRYAEIV
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHH
RERAVMRRLAEVATGIADTAYAPAGRSASQLLDEAEAKVFEIAESGSRGQAGFTEIKPLL
HHHHHHHHHHHHHHCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
KEVVERIDELYHRDNDSGITGVPTGFHDLDQKTSGLQPGDLVIVAGRPSMGKTAFSINIA
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEEECCCCCCCEEEEEEEH
ENVALDTGLPVAVFSMEMGGTQLVMRMIGSVGRLDQHRLRTGKLGEDDWQRLTYALGKLN
HHHECCCCCCEEEEEECCCHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHCCC
EAPVYIDESPGLNVLELRARARRLMRQCGKLGLIVIDYLQLMAGSGGGENRATEISEISR
CCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHH
ALKGLAKELHVPVIALSQLNRGLEQRPNKRPVMSDLRESGAIEQDADVILFIYRDEVYNP
HHHHHHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCEEEEEEECCCCCC
DTQDKGTAEIIISKQRNGPIGTVRLAFLGEYTRFETLAQPGSY
CCCCCCCEEEEEEECCCCCCCEEEHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]