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The map label for this gene is algU [H]

Identifier: 74318113

GI number: 74318113

Start: 2186163

End: 2186759

Strand: Reverse

Name: algU [H]

Synonym: Tbd_2095

Alternate gene names: 74318113

Gene position: 2186759-2186163 (Counterclockwise)

Preceding gene: 74318115

Following gene: 74318112

Centisome position: 75.15

GC content: 59.13

Gene sequence:

>597_bases
ATGTCGGACCGCGAACTGGATCAGATGTTGGTCGAACGCGCGCAGCGCGGCGACAAGCGTGCTTTCGAGTTGTTGGTGAC
GAAGTATCACCGCAAGCTCGGCCGCCTGTTGTCACGCATGGTGCGCGATGCCGCCGAGGTGGAAGATATTACGCAGGAAG
CATTTATCAAGGCTTATCGCGCTTTGCCCGGGTTCCGCGGCGAGAGCGCGTTCTACACCTGGCTGTATCGCATCGCCGTC
AATACCGCCAAGAATTATCTGGTGGCGCACAAGCGCCAGCCGGTATCCAGCGACGTGCTGGCGGAGGATGCGGAAAACTA
CGAAGATGGCGACTTGCTGCGCGACATCGCGACACCCGACGCGGAACTTCAGACCAAGCAGATCGCCAGCGCGGTCAATC
AGGCGGTCGACGCGCTGCCCGAGGAATTGAGGACGGCCATCACATTGCGAGAGATCGAGGGCATGAGCTACGAGGAAATC
GCACAGATGATGGAATGTCCGATCGGAACCGTGCGTTCGCGGATCTTCCGTGCACGCGAGGCCATTGCCGAAAAAATACG
CCCGATGCTGGGCACGAGTCAGGATAAAAGGTGGTAA

Upstream 100 bases:

>100_bases
AAAACCTTGTTTCGGGTGAACTTTTTGCCCGGTTTTCCCTCACACGTTCGGAAGCTGCGGTCACGTATACTTCCAACACA
AACAAGAAACGCAGGGAGAC

Downstream 100 bases:

>100_bases
CCATGTCAGCACTCCACAGGTCCGATGCCTCCTCTCACGCCAACCAGGACGACAACGACGAGTCGCTGTTGTCCGCGCTG
CTCGACGGCGAAAGCGGCCG

Product: RNA polymerase sigma factor RpoE

Products: NA

Alternate protein names: Sigma-30 [H]

Number of amino acids: Translated: 198; Mature: 197

Protein sequence:

>198_residues
MSDRELDQMLVERAQRGDKRAFELLVTKYHRKLGRLLSRMVRDAAEVEDITQEAFIKAYRALPGFRGESAFYTWLYRIAV
NTAKNYLVAHKRQPVSSDVLAEDAENYEDGDLLRDIATPDAELQTKQIASAVNQAVDALPEELRTAITLREIEGMSYEEI
AQMMECPIGTVRSRIFRAREAIAEKIRPMLGTSQDKRW

Sequences:

>Translated_198_residues
MSDRELDQMLVERAQRGDKRAFELLVTKYHRKLGRLLSRMVRDAAEVEDITQEAFIKAYRALPGFRGESAFYTWLYRIAV
NTAKNYLVAHKRQPVSSDVLAEDAENYEDGDLLRDIATPDAELQTKQIASAVNQAVDALPEELRTAITLREIEGMSYEEI
AQMMECPIGTVRSRIFRAREAIAEKIRPMLGTSQDKRW
>Mature_197_residues
SDRELDQMLVERAQRGDKRAFELLVTKYHRKLGRLLSRMVRDAAEVEDITQEAFIKAYRALPGFRGESAFYTWLYRIAVN
TAKNYLVAHKRQPVSSDVLAEDAENYEDGDLLRDIATPDAELQTKQIASAVNQAVDALPEELRTAITLREIEGMSYEEIA
QMMECPIGTVRSRIFRAREAIAEKIRPMLGTSQDKRW

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates genes such as algD, involved in alginate biosynthesis [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=190, Percent_Identity=58.9473684210526, Blast_Score=236, Evalue=1e-63,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 22711; Mature: 22580

Theoretical pI: Translated: 5.47; Mature: 5.47

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDRELDQMLVERAQRGDKRAFELLVTKYHRKLGRLLSRMVRDAAEVEDITQEAFIKAYR
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALPGFRGESAFYTWLYRIAVNTAKNYLVAHKRQPVSSDVLAEDAENYEDGDLLRDIATPD
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHCCCC
AELQTKQIASAVNQAVDALPEELRTAITLREIEGMSYEEIAQMMECPIGTVRSRIFRARE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHH
AIAEKIRPMLGTSQDKRW
HHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SDRELDQMLVERAQRGDKRAFELLVTKYHRKLGRLLSRMVRDAAEVEDITQEAFIKAYR
CHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALPGFRGESAFYTWLYRIAVNTAKNYLVAHKRQPVSSDVLAEDAENYEDGDLLRDIATPD
HCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHCCCC
AELQTKQIASAVNQAVDALPEELRTAITLREIEGMSYEEIAQMMECPIGTVRSRIFRARE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHH
AIAEKIRPMLGTSQDKRW
HHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432708; 8378309; 7961421; 10984043; 7737518 [H]